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hnRNP L (HNRNPL) (NM_001533) Human Tagged ORF Clone
CAT＃: RC219240
HNRNPL (Myc-DDK-tagged)-Human heterogeneous nuclear ribonucleoprotein L (HNRNPL), transcript variant 1
ORF Plasmid: tGFP
"NM_001533" in other vectors (6)
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CNY 6,576.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | hnRNP-L; HNRPL; P/OKcl.14 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC219240 representing NM_001533
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGCGGAGGCTGCTGCCCCGGGCGGAGAAGCGGCGTCGGCGGCTGGAGCAGAGGCAGCAGCCGGACG AGCAGCGGAGGCGGTCGGGAGCGATGGTGAAGATGGCGGCGGCGGGCGGCGGAGGCGGCGGTGGCCGCTA CTACGGCGGCGGCAGTGAGGGCGGCCGGGCCCCTAAGCGGCTCAAGACTGACAACGCCGGCGACCAGCAC GGAGGCGGCGGCGGTGGCGGTGGAGGAGCCGGGGCGGCGGGCGGCGGCGGCGGTGGGGAGAACTACGATG ACCCGCACAAAACCCCTGCCTCCCCAGTTGTCCACATCAGGGGCCTGATTGACGGTGTGGTGGAAGCAGA CCTTGTGGAGGCCTTGCAGGAGTTTGGACCCATCAGCTATGTGGTGGTAATGCCTAAAAAGAGACAAGCA CTGGTGGAGTTTGAAGATGTGTTGGGGGCTTGCAACGCAGTGAACTACGCAGCCGACAACCAAATATACA TTGCTGGTCACCCAGCTTTTGTCAACTACTCTACCAGCCAGAAGATCTCCCGCCCTGGGGACTCGGATGA CTCCCGGAGCGTGAACAGTGTGCTTCTCTTTACCATCCTGAACCCCATTTATTCGATCACCACGGATGTT CTTTACACTATCTGTAATCCTTGTGGCCCTGTCCAGAGAATTGTCATTTTCAGGAAGAATGGAGTTCAGG CGATGGTGGAATTTGACTCAGTTCAAAGTGCCCAGCGGGCCAAGGCCTCTCTCAATGGGGCTGATATCTA TTCTGGCTGTTGCACTCTGAAGATCGAATACGCAAAGCCTACACGCTTGAATGTGTTCAAGAATGATCAG GATACTTGGGACTACACAAACCCCAATCTCAGTGGACAAGGTGACCCTGGCAGCAACCCCAACAAACGCC AGAGGCAGCCCCCTCTCCTGGGAGATCACCCCGCAGAATATGGAGGGCCCCACGGTGGGTACCACAGCCA TTACCATGATGAGGGCTACGGGCCCCCCCCACCTCACTACGAAGGGAGAAGGATGGGTCCACCAGTGGGG GGTCACCGTCGGGGCCCAAGTCGCTACGGCCCCCAGTATGGGCACCCCCCACCCCCTCCCCCACCACCCG AGTATGGCCCTCACGCCGACAGCCCTGTGCTCATGGTCTATGGCTTGGATCAATCTAAGATGAACTGTGA CCGAGTCTTCAATGTCTTCTGCTTATATGGCAATGTGGAGAAGGTGAAATTCATGAAAAGCAAGCCGGGG GCCGCCATGGTGGAGATGGCTGATGGCTACGCTGTAGACCGGGCCATTACCCACCTCAACAACAACTTCA TGTTTGGGCAGAAGCTGAATGTCTGTGTCTCCAAGCAGCCAGCCATCATGCCTGGTCAGTCATACGGGTT GGAAGACGGGTCTTGCAGTTACAAAGACTTCAGTGAATCCCGGAACAATCGGTTCTCCACCCCAGAGCAG GCAGCCAAGAACCGCATCCAGCACCCCAGCAACGTGCTGCACTTCTTCAACGCCCCGCTGGAGGTGACCG AGGAGAACTTCTTTGAGATCTGCGATGAGCTGGGAGTGAAGCGGCCATCTTCTGTGAAAGTATTCTCAGG CAAAAGTGAGCGCAGCTCCTCTGGACTGCTGGAGTGGGAATCCAAGAGCGATGCCCTGGAGACTCTGGGC TTCCTGAACCATTACCAGATGAAAAACCCAAATGGTCCATACCCTTACACTCTGAAGTTGTGTTTCTCCA CTGCTCAGCACGCCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC219240 representing NM_001533
Red=Cloning site Green=Tags(s) MSRRLLPRAEKRRRRLEQRQQPDEQRRRSGAMVKMAAAGGGGGGGRYYGGGSEGGRAPKRLKTDNAGDQH GGGGGGGGGAGAAGGGGGGENYDDPHKTPASPVVHIRGLIDGVVEADLVEALQEFGPISYVVVMPKKRQA LVEFEDVLGACNAVNYAADNQIYIAGHPAFVNYSTSQKISRPGDSDDSRSVNSVLLFTILNPIYSITTDV LYTICNPCGPVQRIVIFRKNGVQAMVEFDSVQSAQRAKASLNGADIYSGCCTLKIEYAKPTRLNVFKNDQ DTWDYTNPNLSGQGDPGSNPNKRQRQPPLLGDHPAEYGGPHGGYHSHYHDEGYGPPPPHYEGRRMGPPVG GHRRGPSRYGPQYGHPPPPPPPPEYGPHADSPVLMVYGLDQSKMNCDRVFNVFCLYGNVEKVKFMKSKPG AAMVEMADGYAVDRAITHLNNNFMFGQKLNVCVSKQPAIMPGQSYGLEDGSCSYKDFSESRNNRFSTPEQ AAKNRIQHPSNVLHFFNAPLEVTEENFFEICDELGVKRPSSVKVFSGKSERSSSGLLEWESKSDALETLG FLNHYQMKNPNGPYPYTLKLCFSTAQHAS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001533 |
| ORF Size | 1767 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001533.2, NP_001524.2 |
| RefSeq Size | 2129 bp |
| RefSeq ORF | 1770 bp |
| Locus ID | 3191 |
| UniProt ID | P14866 |
| Domains | RRM |
| MW | 64 kDa |
| Gene Summary | Heterogeneous nuclear RNAs (hnRNAs) which include mRNA precursors and mature mRNAs are associated with specific proteins to form heterogenous ribonucleoprotein (hnRNP) complexes. Heterogeneous nuclear ribonucleoprotein L is among the proteins that are stably associated with hnRNP complexes and along with other hnRNP proteins is likely to play a major role in the formation, packaging, processing, and function of mRNA. Heterogeneous nuclear ribonucleoprotein L is present in the nucleoplasm as part of the HNRP complex. HNRP proteins have also been identified outside of the nucleoplasm. Exchange of hnRNP for mRNA-binding proteins accompanies transport of mRNA from the nucleus to the cytoplasm. Since HNRP proteins have been shown to shuttle between the nucleus and the cytoplasm, it is possible that they also have cytoplasmic functions. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219240L1 | Lenti ORF clone of Human heterogeneous nuclear ribonucleoprotein L (HNRNPL), transcript variant 1, Myc-DDK-tagged |
CNY 8,976.00 |
|
| RC219240L2 | Lenti ORF clone of Human heterogeneous nuclear ribonucleoprotein L (HNRNPL), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RC219240L3 | Lenti ORF clone of Human heterogeneous nuclear ribonucleoprotein L (HNRNPL), transcript variant 1, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC219240L4 | Lenti ORF clone of Human heterogeneous nuclear ribonucleoprotein L (HNRNPL), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RG219240 | HNRNPL (tGFP-tagged) - Human heterogeneous nuclear ribonucleoprotein L (HNRNPL), transcript variant 1 |
CNY 8,176.00 |
|
| SC314118 | HNRNPL (untagged)-Human heterogeneous nuclear ribonucleoprotein L (HNRNPL), transcript variant 1 |
CNY 6,592.00 |
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