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UBR1 (NM_174916) Human Tagged ORF Clone
CAT＃: RC219454
UBR1 (Myc-DDK-tagged)-Human ubiquitin protein ligase E3 component n-recognin 1 (UBR1)
ORF Plasmid: tGFP
"NM_174916" in other vectors (4)
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CNY 17,632.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | JBS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC219454 representing NM_174916
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGACGAGGAGGCTGGAGGTACTGAGAGGATGGAAATCAGCGCGGAGTTACCCCAGACCCCTCAGC GTCTGGCATCTTGGTGGGATCAGCAAGTTGATTTTTATACTGCTTTCTTGCATCATTTGGCACAATTGGT GCCAGAAATTTACTTTGCTGAAATGGACCCAGACTTGGAAAAGCAGGAGGAAAGTGTACAAATGTCAATA TTCACTCCACTGGAATGGTACTTATTTGGAGAAGATCCAGATATTTGCTTAGAGAAATTGAAGCACAGTG GAGCATTTCAGCTTTGTGGGAGGGTTTTCAAAAGTGGAGAGACAACCTATTCTTGCAGGGATTGTGCAAT TGATCCAACATGTGTACTCTGTATGGACTGCTTCCAGGACAGTGTTCATAAAAATCATCGTTACAAGATG CATACTTCTACTGGAGGAGGGTTCTGTGACTGTGGAGACACAGAGGCATGGAAAACTGGCCCTTTTTGTG TAAATCATGAACCTGGAAGAGCAGGTACTATAAAAGAGAATTCACGCTGTCCGTTGAATGAAGAGGTAAT TGTCCAAGCCAGGAAAATATTTCCTTCAGTGATAAAATATGTCGTAGAAATGACTATATGGGAAGAGGAA AAAGAACTGCCTCCTGAACTCCAGATAAGGGAGAAAAATGAAAGATACTATTGTGTCCTTTTCAATGATG AACACCATTCATATGACCACGTCATATACAGCCTACAAAGAGCTCTTGACTGTGAGCTCGCAGAGGCCCA GTTGCATACCACTGCCATTGACAAAGAGGGTCGTCGGGCTGTTAAAGCGGGAGCTTATGCTGCTTGCCAG GAAGCAAAGGAAGATATAAAGAGTCATTCAGAAAATGTCTCTCAACATCCACTTCATGTAGAAGTATTAC ACTCAGAGATTATGGCTCATCAGAAATTTGCTTTGCGTCTTGGTTCCTGGATGAACAAAATTATGAGCTA TTCAAGTGACTTTAGGCAGATCTTTTGCCAAGCATGCCTTAGAGAAGAACCTGACTCGGAGAATCCCTGT CTCATAAGCAGGTTAATGCTTTGGGATGCAAAGCTTTATAAAGGTGCCCGTAAGATCCTTCATGAATTGA TCTTCAGCAGTTTTTTTATGGAGATGGAATACAAAAAACTCTTTGCTATGGAATTTGTGAAGTATTATAA ACAACTGCAGAAAGAATATATCAGTGATGATCATGACAGAAGTATCTCTATAACTGCACTTTCAGTTCAG ATGTTTACTGTTCCTACTCTGGCTCGACATCTTATTGAAGAGCAGAATGTTATCTCTGTCATTACTGAAA CTCTGCTAGAAGTTTTACCTGAGTACTTGGACAGGAACAATAAATTCAACTTCCAGGGTTATAGCCAGGA CAAATTGGGAAGAGTATATGCAGTAATATGTGACCTAAAGTATATCCTGATCAGCAAACCCACAATATGG ACAGAAAGATTAAGAATGCAGTTCCTTGAAGGTTTTCGATCTTTTTTGAAGATTCTTACCTGTATGCAGG GAATGGAAGAAATCCGAAGACAGGTTGGGCAACACATTGAAGTGGATCCTGATTGGGAGGCTGCCATTGC TATACAGATGCAATTGAAGAATATTTTACTCATGTTCCAAGAGTGGTGTGCTTGTGATGAAGAACTCTTA CTTGTGGCTTATAAAGAATGTCACAAAGCTGTGATGAGGTGCAGTACCAGTTTCATATCTAGTAGCAAGA CAGTAGTACAATCGTGTGGACATAGTTTGGAAACAAAGTCCTACAGAGTATCTGAGGATCTTGTAAGCAT ACATCTGCCACTCTCTAGGACCCTTGCTGGTCTTCATGTACGTTTAAGCAGGCTGGGTGCTGTTTCAAGA CTGCATGAATTTGTGTCTTTTGAGGACTTTCAAGTAGAGGTACTAGTGGAATATCCTTTACGTTGTCTGG TGTTGGTTGCCCAGGTTGTTGCTGAGATGTGGCGAAGAAATGGACTGTCTCTTATTAGCCAGGTGTTTTA TTACCAAGATGTTAAGTGCAGAGAAGAAATGTATGATAAAGATATCATCATGCTTCAGATTGGTGCATCT TTAATGGATCCCAATAAGTTCTTGTTACTGGTACTTCAGAGGTATGAACTTGCCGAGGCTTTTAACAAGA CCATATCTACAAAAGACCAGGATTTGATTAAACAATATAATACACTAATAGAAGAAATGCTTCAGGTCCT CATCTATATTGTGGGTGAGCGTTATGTACCTGGAGTGGGAAATGTGACCAAAGAAGAGGTCACAATGAGA GAAATCATTCACTTGCTTTGCATTGAACCCATGCCACACAGTGCCATTGCCAAAAATTTACCTGAGAATG AAAATAATGAAACTGGCTTAGAGAATGTCATAAACAAAGTGGCCACATTTAAGAAACCAGGTGTATCAGG CCATGGAGTTTATGAACTAAAAGATGAATCACTGAAAGACTTCAATATGTACTTTTATCATTACTCCAAA ACCCAGCATAGCAAGGCTGAACATATGCAGAAGAAAAGGAGAAAACAAGAAAACAAAGATGAAGCATTGC CGCCACCACCACCTCCTGAATTCTGCCCTGCTTTCAGCAAAGTGATTAACCTTCTCAACTGTGATATCAT GATGTACATTCTCAGGACCGTATTTGAGCGGGCAATAGACACAGATTCTAACTTGTGGACCGAAGGGATG CTCCAAATGGCTTTTCATATTCTGGCATTGGGTTTACTAGAAGAGAAGCAACAGCTTCAAAAAGCTCCTG AAGAAGAAGTAACATTTGACTTTTATCATAAGGCTTCAAGATTGGGAAGTTCAGCCATGAATATACAAAT GCTTTTGGAAAAACTCAAAGGAATTCCCCAGTTAGAAGGCCAGAAGGACATGATAACGTGGATACTTCAG ATGTTTGACACAGTGAAGCGATTAAGAGAAAAATCTTGTTTAATTGTAGCAACCACATCAGGATCGGAAT CTATTAAGAATGATGAGATTACTCATGATAAAGAAAAAGCAGAACGAAAAAGAAAAGCTGAAGCTGCTAG GCTACATCGCCAGAAGATCATGGCTCAGATGTCTGCCTTACAGAAAAACTTCATTGAAACTCATAAACTC ATGTATGACAATACATCAGAAATGCCTGGGAAAGAAGATTCCATTATGGAGGAAGAGAGCACCCCAGCAG TCAGTGACTACTCTAGAATTGCTTTGGGTCCTAAACGGGGTCCATCTGTTACTGAAAAGGAGGTGCTGAC GTGCATCCTTTGCCAAGAAGAACAGGAGGTGAAAATAGAAAATAATGCCATGGTATTATCGGCCTGTGTC CAGAAATCTACTGCCTTAACCCAGCACAGGGGAAAACCCATAGAACTCTCAGGAGAAGCCCTAGACCCAC TTTTCATGGATCCAGACTTGGCATATGGAACTTATACAGGAAGCTGTGGTCATGTAATGCACGCAGTGTG CTGGCAGAAGTATTTTGAAGCTGTACAGCTGAGCTCTCAGCAGCGCATTCATGTTGACCTTTTTGACTTG GAAAGTGGAGAATATCTTTGCCCTCTTTGCAAATCTCTGTGCAATACTGTGATCCCCATTATTCCTTTGC AACCTCAAAAGATAAACAGTGAGAATGCAGATGCTCTTGCTCAACTTTTGACCCTGGCACGGTGGATACA GACTGTTCTGGCCAGAATATCAGGTTATAATATAAGACATGCTAAAGGAGAAAACCCAATTCCTATTTTC TTTAATCAAGGAATGGGAGATTCTACTTTGGAGTTCCATTCCATCCTGAGTTTTGGCGTTGAGTCTTCGA TTAAATATTCAAATAGCATCAAGGAAATGGTTATTCTCTTTGCCACAACAATTTATAGAATTGGATTGAA AGTGCCACCTGATGAAAGGGATCCTCGAGTCCCCATGCTGACCTGGAGCACCTGCGCTTTCACTATCCAG GCAATTGAAAATCTATTGGGAGATGAAGGAAAACCTCTGTTTGGAGCACTTCAAAATAGGCAGCATAATG GTCTGAAAGCATTAATGCAGTTTGCAGTTGCACAGAGGATTACCTGTCCTCAGGTCCTGATACAGAAACA TCTGGTTCGTCTTCTATCAGTTGTTCTTCCTAACATAAAATCAGAAGATACACCATGCCTTCTGTCTATA GATCTGTTTCATGTTTTGGTGGGTGCTGTGTTAGCATTCCCATCCTTGTATTGGGATGACCCTGTTGATC TGCAGCCTTCTTCAGTTAGTTCTTCCTATAACCACCTTTATCTCTTCCATTTGATCACCATGGCACACAT GCTTCAGATACTACTTACAGTAGACACAGGCCTACCCCTTGCTCAGGTTCAAGAAGACAGTGAAGAGGCT CATTCCGCATCTTCTTTCTTTGCAGAAATTTCTCAATATACAAGTGGCTCCATTGGGTGTGATATTCCTG GCTGGTATTTGTGGGTCTCACTGAAGAATGGCATCACCCCTTATCTTCGCTGTGCTGCATTGTTTTTCCA CTATTTACTTGGGGTAACTCCGCCTGAGGAACTGCATACCAATTCTGCAGAAGGAGAGTACAGTGCACTC TGTAGCTATCTATCTTTACCTACAAATTTGTTCCTGCTCTTCCAGGAATATTGGGATACTGTAAGGCCCT TGCTCCAGAGGTGGTGTGCAGATCCTGCCTTACTAAACTGTTTGAAGCAAAAAAACACCGTGGTCAGGTA CCCTAGAAAAAGAAATAGTTTGATAGAGCTTCCTGATGACTATAGCTGCCTCCTGAATCAAGCTTCTCAT TTCAGGTGCCCACGGTCTGCAGATGATGAGCGAAAGCATCCTGTCCTCTGCCTTTTCTGTGGGGCTATAC TATGTTCTCAGAACATTTGCTGCCAGGAAATTGTGAACGGGGAAGAGGTTGGAGCTTGCATTTTTCACGC ACTTCACTGTGGAGCCGGAGTCTGCATTTTCCTAAAAATCAGAGAATGCCGAGTGGTCCTGGTTGAAGGT AAAGCCAGAGGCTGTGCCTATCCAGCTCCTTACTTGGATGAATATGGAGAAACAGACCCTGGCCTGAAGA GGGGCAACCCCCTTCATTTATCTCGTGAGCGGTATCGGAAGCTCCATTTGGTCTGGCAACAACACTGCAT TATAGAAGAGATTGCTAGGAGCCAAGAGACTAATCAGATGTTATTTGGATTCAACTGGCAGTTACTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC219454 representing NM_174916
Red=Cloning site Green=Tags(s) MADEEAGGTERMEISAELPQTPQRLASWWDQQVDFYTAFLHHLAQLVPEIYFAEMDPDLEKQEESVQMSI FTPLEWYLFGEDPDICLEKLKHSGAFQLCGRVFKSGETTYSCRDCAIDPTCVLCMDCFQDSVHKNHRYKM HTSTGGGFCDCGDTEAWKTGPFCVNHEPGRAGTIKENSRCPLNEEVIVQARKIFPSVIKYVVEMTIWEEE KELPPELQIREKNERYYCVLFNDEHHSYDHVIYSLQRALDCELAEAQLHTTAIDKEGRRAVKAGAYAACQ EAKEDIKSHSENVSQHPLHVEVLHSEIMAHQKFALRLGSWMNKIMSYSSDFRQIFCQACLREEPDSENPC LISRLMLWDAKLYKGARKILHELIFSSFFMEMEYKKLFAMEFVKYYKQLQKEYISDDHDRSISITALSVQ MFTVPTLARHLIEEQNVISVITETLLEVLPEYLDRNNKFNFQGYSQDKLGRVYAVICDLKYILISKPTIW TERLRMQFLEGFRSFLKILTCMQGMEEIRRQVGQHIEVDPDWEAAIAIQMQLKNILLMFQEWCACDEELL LVAYKECHKAVMRCSTSFISSSKTVVQSCGHSLETKSYRVSEDLVSIHLPLSRTLAGLHVRLSRLGAVSR LHEFVSFEDFQVEVLVEYPLRCLVLVAQVVAEMWRRNGLSLISQVFYYQDVKCREEMYDKDIIMLQIGAS LMDPNKFLLLVLQRYELAEAFNKTISTKDQDLIKQYNTLIEEMLQVLIYIVGERYVPGVGNVTKEEVTMR EIIHLLCIEPMPHSAIAKNLPENENNETGLENVINKVATFKKPGVSGHGVYELKDESLKDFNMYFYHYSK TQHSKAEHMQKKRRKQENKDEALPPPPPPEFCPAFSKVINLLNCDIMMYILRTVFERAIDTDSNLWTEGM LQMAFHILALGLLEEKQQLQKAPEEEVTFDFYHKASRLGSSAMNIQMLLEKLKGIPQLEGQKDMITWILQ MFDTVKRLREKSCLIVATTSGSESIKNDEITHDKEKAERKRKAEAARLHRQKIMAQMSALQKNFIETHKL MYDNTSEMPGKEDSIMEEESTPAVSDYSRIALGPKRGPSVTEKEVLTCILCQEEQEVKIENNAMVLSACV QKSTALTQHRGKPIELSGEALDPLFMDPDLAYGTYTGSCGHVMHAVCWQKYFEAVQLSSQQRIHVDLFDL ESGEYLCPLCKSLCNTVIPIIPLQPQKINSENADALAQLLTLARWIQTVLARISGYNIRHAKGENPIPIF FNQGMGDSTLEFHSILSFGVESSIKYSNSIKEMVILFATTIYRIGLKVPPDERDPRVPMLTWSTCAFTIQ AIENLLGDEGKPLFGALQNRQHNGLKALMQFAVAQRITCPQVLIQKHLVRLLSVVLPNIKSEDTPCLLSI DLFHVLVGAVLAFPSLYWDDPVDLQPSSVSSSYNHLYLFHLITMAHMLQILLTVDTGLPLAQVQEDSEEA HSASSFFAEISQYTSGSIGCDIPGWYLWVSLKNGITPYLRCAALFFHYLLGVTPPEELHTNSAEGEYSAL CSYLSLPTNLFLLFQEYWDTVRPLLQRWCADPALLNCLKQKNTVVRYPRKRNSLIELPDDYSCLLNQASH FRCPRSADDERKHPVLCLFCGAILCSQNICCQEIVNGEEVGACIFHALHCGAGVCIFLKIRECRVVLVEG KARGCAYPAPYLDEYGETDPGLKRGNPLHLSRERYRKLHLVWQQHCIIEEIARSQETNQMLFGFNWQLL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_174916 |
| ORF Size | 5247 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_174916.3 |
| RefSeq Size | 7758 bp |
| RefSeq ORF | 5250 bp |
| Locus ID | 197131 |
| UniProt ID | Q8IWV7 |
| Protein Families | Druggable Genome |
| MW | 200 kDa |
| Gene Summary | The N-end rule pathway is one proteolytic pathway of the ubiquitin system. The recognition component of this pathway, encoded by this gene, binds to a destabilizing N-terminal residue of a substrate protein and participates in the formation of a substrate-linked multiubiquitin chain. This leads to the eventual degradation of the substrate protein. The protein described in this record has a RING-type zinc finger and a UBR-type zinc finger. Mutations in this gene have been associated with Johanson-Blizzard syndrome. [provided by RefSeq, Jul 2008] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219454L1 | Lenti ORF clone of Human ubiquitin protein ligase E3 component n-recognin 1 (UBR1), Myc-DDK-tagged |
CNY 19,380.00 |
|
| RC219454L3 | Lenti ORF clone of Human ubiquitin protein ligase E3 component n-recognin 1 (UBR1), Myc-DDK-tagged |
CNY 19,380.00 |
|
| RG219454 | UBR1 (tGFP-tagged) - Human ubiquitin protein ligase E3 component n-recognin 1 (UBR1) |
CNY 19,190.00 |
|
| SC309224 | UBR1 (untagged)-Human ubiquitin protein ligase E3 component n-recognin 1 (UBR1) |
CNY 26,790.00 |
