MPG (NM_002434) Human Tagged ORF Clone

CAT#: RC219602

  • TrueORF®

MPG (Myc-DDK-tagged)-Human N-methylpurine-DNA glycosylase (MPG), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_002434" in other vectors (4)

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CNY 3,990.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal Anti-MPG Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms AAG; ADPG; anpg; APNG; CRA36.1; MDG; Mid1; PIG11; PIG16
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC219602 representing NM_002434
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTCACCCCCGCTTTGCAGATGAAGAAACCAAAGCAGTTTTGCCGACGGATGGGGCAAAAGAAGCAGC
GACCAGCTAGAGCAGGGCAGCCACACAGCTCGTCCGACGCAGCCCAGGCACCTGCAGAGCAGCCACACAG
CTCGTCCGATGCAGCCCAGGCACCTTGCCCCAGGGAGCGCTGCTTGGGACCGCCCACCACTCCGGGCCCA
TACCGCAGCATCTATTTCTCAAGCCCAAAGGGCCACCTTACCCGACTGGGGTTGGAGTTCTTCGACCAGC
CGGCAGTCCCCCTGGCCCGGGCATTTCTGGGACAGGTCCTAGTCCGGCGACTTCCTAATGGCACAGAACT
CCGAGGCCGCATCGTGGAGACCGAGGCATACCTGGGGCCAGAGGATGAAGCCGCCCACTCAAGGGGTGGC
CGGCAGACCCCCCGCAACCGAGGCATGTTCATGAAGCCGGGGACCCTGTACGTGTACATCATTTACGGCA
TGTACTTCTGCATGAACATCTCCAGCCAGGGGGACGGGGCTTGCGTCTTGCTGCGAGCACTGGAGCCCCT
GGAAGGTCTGGAGACCATGCGTCAGCTTCGCAGCACCCTCCGGAAAGGCACCGCCAGCCGTGTCCTCAAG
GACCGCGAGCTCTGCAGTGGCCCCTCCAAGCTGTGCCAGGCCCTGGCCATCAACAAGAGCTTTGACCAGA
GGGACCTGGCACAGGATGAAGCTGTATGGCTGGAGCGTGGTCCCCTGGAGCCCAGTGAGCCGGCTGTAGT
GGCAGCAGCCCGGGTGGGCGTCGGCCATGCAGGGGAGTGGGCCCGGAAACCCCTCCGCTTCTATGTCCGG
GGCAGCCCCTGGGTCAGTGTGGTCGACAGAGTGGCTGAGCAGGACACACAGGCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC219602 representing NM_002434
Red=Cloning site Green=Tags(s)

MVTPALQMKKPKQFCRRMGQKKQRPARAGQPHSSSDAAQAPAEQPHSSSDAAQAPCPRERCLGPPTTPGP
YRSIYFSSPKGHLTRLGLEFFDQPAVPLARAFLGQVLVRRLPNGTELRGRIVETEAYLGPEDEAAHSRGG
RQTPRNRGMFMKPGTLYVYIIYGMYFCMNISSQGDGACVLLRALEPLEGLETMRQLRSTLRKGTASRVLK
DRELCSGPSKLCQALAINKSFDQRDLAQDEAVWLERGPLEPSEPAVVAAARVGVGHAGEWARKPLRFYVR
GSPWVSVVDRVAEQDTQA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_002434
ORF Size 894 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002434.4
RefSeq Size 1289 bp
RefSeq ORF 897 bp
Locus ID 4350
UniProt ID P29372
Protein Families Druggable Genome, Transmembrane
Protein Pathways Base excision repair
MW 32.9 kDa
Gene Summary Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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