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PDE11A (NM_016953) Human Tagged ORF Clone
CAT＃: RC220304
PDE11A (Myc-DDK-tagged)-Human phosphodiesterase 11A (PDE11A), transcript variant 4
ORF Plasmid: tGFP
"NM_016953" in other vectors (6)
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CNY 6,840.00
CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PPNAD2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC220304 representing NM_016953
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCCTCCCGCCTGGACTTTGGGGAGGTGGAAACTTTCCTGGACAGGCACCCAGAGTTGTTTGAAG ATTACTTGATGCGGAAGGGGAAGCAGGAGATGGTTGAAAAGTGGCTGCAGAGGCACAGTCAGGGTCAGGG GGCTTTAGGTCCAAGGCCCTCTTTGGCTGGTACCAGCAGCTTGGCTCACAGCACCTGCAGAGGTGGCAGC AGCGTTGGTGGTGGCACTGGACCAAATGGCTCTGCCCACAGCCAGCCCCTTCCCGGTGGCGGGGACTGTG GTGGGGTTCCCTTGAGTCCCAGCTGGGCCGGTGGCAGCAGGGGCGATGGGAACCTGCAGCGGAGAGCTTC TCAGAAAGAGCTAAGGAAGAGTTTTGCCCGCTCCAAGGCCATCCACGTGAACAGGACCTACGATGAACAG GTGACCTCCCGGGCTCAGGAACCCCTGAGTAGTGTACGACGGAGGGCACTTCTCCGGAAGGCAAGCTCCC TGCCCCCCACCACAGCCCATATTCTCAGTGCGCTGCTGGAATCGAGAGTGAATCTGCCTCAGTATCCCCC TACAGCCATCGACTACAAGTGCCATCTGAAAAAGCATAATGAGCGTCAGTTCTTTCTGGAATTGGTCAAA GATATCTCCAATGACCTTGACCTCACCAGCCTGAGCTACAAGATTCTCATCTTTGTCTGCCTTATGGTGG ATGCTGACCGCTGCTCTCTTTTCCTGGTGGAAGGGGCAGCTGCTGGCAAGAAGACCTTGGTCTCCAAATT CTTTGATGTGCATGCAGGAACCCCTCTGCTGCCTTGCAGCAGCACAGAGAACTCAAATGAGGTGCAGGTC CCCTGGGGCAAAGGTATCATTGGCTATGTCGGGGAGCATGGAGAAACGGTCAACATTCCTGATGCCTACC AGGATCGACGATTCAATGATGAAATCGACAAGCTAACTGGATACAAGACAAAATCATTATTGTGCATGCC TATCCGAAGCAGTGATGGTGAGATTATTGGTGTGGCCCAAGCGATAAATAAGATTCCTGAAGGAGCTCCA TTTACTGAAGATGATGAAAAAGTTATGCAGATGTATCTTCCATTTTGTGGAATCGCCATATCTAACGCTC AGCTCTTTGCTGCCTCAAGGAAAGAATATGAAAGAAGCAGAGCTTTGCTAGAGGTGGTTAATGACCTCTT TGAAGAACAGACTGACCTGGAGAAAATTGTCAAGAAAATAATGCATCGGGCCCAAACTCTGCTGAAATGT GAACGCTGTTCTGTTTTACTCCTAGAGGACATCGAATCACCAGTGGTGAAATTTACCAAATCCTTTGAAT TGATGTCCCCAAAGTGCAGTGCTGATGCTGAGAACAGTTTCAAAGAAAGCATGGAGAAATCATCATACTC CGACTGGCTAATAAATAACAGCATTGCTGAGCTGGTTGCTTCAACAGGCCTTCCAGTGAACATCAGTGAT GCCTACCAGGATCCGCGCTTTGATGCAGAGGCAGACCAGATATCTGGTTTTCACATAAGATCTGTTCTTT GTGTCCCTATTTGGAATAGCAACCACCAAATAATTGGAGTGGCTCAAGTGTTAAACAGACTTGATGGGAA ACCTTTTGATGATGCAGATCAACGACTTTTTGAGGCTTTTGTCATCTTTTGTGGACTTGGCATCAACAAC ACAATTATGTATGATCAAGTGAAGAAGTCCTGGGCCAAGCAGTCTGTGGCTCTTGATGTGCTATCATACC ATGCAACATGTTCAAAAGCTGAAGTTGACAAGTTTAAGGCAGCCAACATCCCTCTGGTGTCAGAACTTGC CATCGATGACATTCATTTTGATGACTTTTCTCTCGACGTTGATGCCATGATCACAGCTGCTCTCCGGATG TTCATGGAGCTGGGGATGGTACAGAAATTTAAAATTGACTATGAGACACTGTGTAGGTGGCTTTTGACAG TGAGGAAAAACTATCGGATGGTTCTATACCACAACTGGAGACATGCCTTCAACGTGTGTCAGCTGATGTT CGCGATGTTAACCACTGCTGGGTTTCAAGACATTCTGACCGAGGTGGAAATTTTAGCGGTGATTGTGGGA TGCCTGTGTCATGACCTCGACCACAGGGGAACCAACAATGCCTTCCAAGCTAAGAGTGGCTCTGCCCTGG CCCAACTCTATGGAACCTCTGCTACCTTGGAGCATCACCATTTCAACCACGCCGTGATGATCCTTCAAAG TGAGGGTCACAATATCTTTGCTAACCTGTCCTCCAAGGAATATAGTGACCTTATGCAGCTTTTGAAGCAG TCAATATTGGCAACAGACCTCACGCTGTACTTTGAGAGGAGAACTGAATTCTTTGAACTTGTCAGTAAAG GAGAATACGATTGGAACATCAAAAACCATCGTGATATATTTCGATCAATGTTAATGACAGCCTGTGACCT TGGAGCCGTGACCAAACCGTGGGAGATCTCCAGACAGGTGGCAGAACTTGTAACCAGTGAGTTCTTCGAA CAAGGAGATCGGGAGAGATTAGAGCTCAAACTCACTCCTTCAGCAATTTTTGATCGGAACCGGAAGGATG AACTGCCTCGGTTGCAACTGGAGTGGATTGATAGCATCTGCATGCCTTTGTATCAGGCACTGGTGAAGGT CAACGTGAAACTGAAGCCGATGCTAGATTCAGTAGCTACAAACAGAAGTAAGTGGGAAGAGCTACACCAA AAACGACTGCTGGCCTCAACTGCCTCATCCTCCCCTGCCAGTGTTATGGTAGCCAAGGAAGACAGGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC220304 representing NM_016953
Red=Cloning site Green=Tags(s) MAASRLDFGEVETFLDRHPELFEDYLMRKGKQEMVEKWLQRHSQGQGALGPRPSLAGTSSLAHSTCRGGS SVGGGTGPNGSAHSQPLPGGGDCGGVPLSPSWAGGSRGDGNLQRRASQKELRKSFARSKAIHVNRTYDEQ VTSRAQEPLSSVRRRALLRKASSLPPTTAHILSALLESRVNLPQYPPTAIDYKCHLKKHNERQFFLELVK DISNDLDLTSLSYKILIFVCLMVDADRCSLFLVEGAAAGKKTLVSKFFDVHAGTPLLPCSSTENSNEVQV PWGKGIIGYVGEHGETVNIPDAYQDRRFNDEIDKLTGYKTKSLLCMPIRSSDGEIIGVAQAINKIPEGAP FTEDDEKVMQMYLPFCGIAISNAQLFAASRKEYERSRALLEVVNDLFEEQTDLEKIVKKIMHRAQTLLKC ERCSVLLLEDIESPVVKFTKSFELMSPKCSADAENSFKESMEKSSYSDWLINNSIAELVASTGLPVNISD AYQDPRFDAEADQISGFHIRSVLCVPIWNSNHQIIGVAQVLNRLDGKPFDDADQRLFEAFVIFCGLGINN TIMYDQVKKSWAKQSVALDVLSYHATCSKAEVDKFKAANIPLVSELAIDDIHFDDFSLDVDAMITAALRM FMELGMVQKFKIDYETLCRWLLTVRKNYRMVLYHNWRHAFNVCQLMFAMLTTAGFQDILTEVEILAVIVG CLCHDLDHRGTNNAFQAKSGSALAQLYGTSATLEHHHFNHAVMILQSEGHNIFANLSSKEYSDLMQLLKQ SILATDLTLYFERRTEFFELVSKGEYDWNIKNHRDIFRSMLMTACDLGAVTKPWEISRQVAELVTSEFFE QGDRERLELKLTPSAIFDRNRKDELPRLQLEWIDSICMPLYQALVKVNVKLKPMLDSVATNRSKWEELHQ KRLLASTASSSPASVMVAKEDRN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016953 |
| ORF Size | 2802 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_016953.4 |
| RefSeq Size | 9305 bp |
| RefSeq ORF | 2802 bp |
| Locus ID | 50940 |
| UniProt ID | Q9HCR9 |
| Domains | PDEase, GAF, HDc |
| Protein Families | Druggable Genome |
| Protein Pathways | Progesterone-mediated oocyte maturation, Purine metabolism |
| MW | 104.6 kDa |
| Gene Summary | The 3',5'-cyclic nucleotides cAMP and cGMP function as second messengers in a wide variety of signal transduction pathways. 3',5'-cyclic nucleotide phosphodiesterases (PDEs) catalyze the hydrolysis of cAMP and cGMP to the corresponding 5'-monophosphates and provide a mechanism to downregulate cAMP and cGMP signaling. This gene encodes a member of the PDE protein superfamily. Mutations in this gene are a cause of Cushing disease and adrenocortical hyperplasia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220304L1 | Lenti ORF clone of Human phosphodiesterase 11A (PDE11A), transcript variant 4, Myc-DDK-tagged |
CNY 9,240.00 |
|
| RC220304L2 | Lenti ORF clone of Human phosphodiesterase 11A (PDE11A), transcript variant 4, mGFP tagged |
CNY 9,030.00 |
|
| RC220304L3 | Lenti ORF clone of Human phosphodiesterase 11A (PDE11A), transcript variant 4, Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC220304L4 | Lenti ORF clone of Human phosphodiesterase 11A (PDE11A), transcript variant 4, mGFP tagged |
CNY 9,030.00 |
|
| RG220304 | PDE11A (tGFP-tagged) - Human phosphodiesterase 11A (PDE11A), transcript variant 4 |
CNY 7,890.00 |
|
| SC125346 | PDE11A (untagged)-Human phosphodiesterase 11A (PDE11A), transcript variant 4 |
CNY 6,848.00 |
