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FBXL18 (NM_024963) Human Tagged ORF Clone
CAT＃: RC220401
FBXL18 (Myc-DDK-tagged)-Human F-box and leucine-rich repeat protein 18 (FBXL18)
ORF Plasmid: tGFP
"NM_024963" in other vectors (4)
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CNY 5,256.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Fbl18 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC220401 representing NM_024963
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGCTCCGGAGAGGACATATCCAATGATGATGATGACATGCACCCTGCAGCAGCCGGGATGGCAG ACGGGGTCCACCTCCTAGGGTTCTCTGATGAGATCCTCCTTCACATCCTGAGTCACGTCCCCAGCACAGA TCTGATTCTGAACGTCCGGCGTACCTGTCGGAAGCTTGCAGCCCTGTGCCTTGACAAGAGCCTCATCCAC ACCGTGTTGCTGCAAAAGGACTATCAGGCGAGCGAGGACAAAGTGAGGCAGCTGGTGAAGGAGATCGGCC GGGAGATCCAGCAGCTGAGCATGGCTGGCTGCTACTGGCTGCCTGGCTCCACCGTGGAACACGTGGCCCG CTGCCGCAGCCTGGTGAAGGTGAACCTCTCGGGCTGCCACCTCACTTCCCTGCGCCTCTCCAAGATGCTC TCGGCCCTGCAGCACCTGCGCTCGCTGGCCATCGACGTGAGCCCCGGCTTCGACGCCAGCCAGCTGAGCA GCGAGTGCAAGGCCACCCTGAGCCGCGTGCGGGAGCTCAAGCAGACGCTGTTCACTCCCTCCTACGGCGT GGTGCCCTGCTGCACCAGCCTAGAGAAGCTGCTGCTCTACTTCGAGATTCTGGACCGCACGCGCGAGGGC GCCATCCTCTCGGGCCAGCTTATGGTGGGCCAGAGCAACGTGCCGCACTACCAGAACCTGCGGGTCTTCT ATGCGCGCCTGGCCCCCGGCTACATCAACCAGGAGGTGGTGCGGCTCTACCTGGCTGTGCTTAGCGACCG CACTCCTCAGAACCTCCACGCCTTCCTCATCTCCGTCCCTGGCAGCTTCGCGGAGAGCGGCGCCACCAAG AACCTCCTGGACTCCATGGCGCGCAATGTCGTGCTGGATGCCCTGCAGCTGCCCAAGTCCTGGCTGAACG GCTCTTCCCTCCTGCAGCACATGAAATTCAACAACCCGTTCTACTTCAGTTTCAGCCGCTGTACCCTGTC AGGCGGCCATCTGATCCAGCAGGTCATCAACGGCGGGAAGGACCTGCGGAGCCTGGCCAGCTTGAACCTC AGCGGCTGCGTCCACTGCCTGTCCCCAGACTCGCTGCTCCGCAAGGCGGAGGACGACATCGACAGCAGCA TCCTGGAGACTCTGGTGGCGTCCTGCTGCAACCTGCGCCACCTGAACCTCTCGGCCGCCCACCACCACAG CTCGGAGGGCCTGGGCCGCCACCTCTGCCAGCTCCTGGCCCGGCTGCGTCACCTGCGCTCCCTCTCCCTG CCTGTCTGCTCTGTCGCTGACTCCGCGCCGCGCGCCGACCGCGCGCCCGCCCAGCCGGCCATGCACGCAG TGCCGCGCGGCTTTGGCAAGAAAGTGCGTGTGGGCGTGCAGTCCTGTCCCAGCCCCTTCTCGGGCCAGGC GTGCCCCCAGCCCTCCTCCGTGTTCTGGTCTCTGCTGAAGAACCTGCCCTTCCTGGAACACCTCGAGCTG ATTGGGTCCAACTTCTCCTCCGCCATGCCCCGCAACGAGCCCGCCATCCGCAACTCGCTCCCACCCTGCA GCCGCGCACAGAGTGTCGGGGACTCGGAGGTGGCCGCCATCGGCCAGCTGGCCTTCCTGCGGCACCTGAC GCTCGCACAGCTGCCCAGCGTCCTTACGGGCTCCGGGCTGGTCAATATCGGCCTGCAGTGCCAGCAGTTG CGGTCCCTGTCGCTGGCCAACCTGGGCATGATGGGGAAGGTGGTGTACATGCCCGCGCTCTCAGACATGT TGAAGCACTGCAAGCGGCTGAGGGACCTCAGGCTGGAGCAGCCCTACTTCAGCGCCAACGCCCAGTTCTT CCAGGCGCTGAGCCAGTGCCCCTCGCTGCAGCGCCTGTGCCTGGTCTCTCGCAGCGGCACCCTCCAGCCC GATGCCGTGCTGGCCTTCATGGCTCGCTGCCTGCAGGTTGTCATGTGCCACCTGTTCACCGGGGAGTCCC TCGCCACCTGCAAGAGCCTGCAGCAGTCGCTTCTCCGCAGCTTCCAGGCCGAGCGGCCCGCGTTAAACGT CGTCATCTTCCCTCTGCTCCACGAGGGCCTGACCGACGTCATCCGGGACGTCCCCCTGGTGCACCTGGAT GAGATCACCTTATTTAAGAGCAGAGTGGCCGAGGAACCGCCGAACCTGTGGTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC220401 representing NM_024963
Red=Cloning site Green=Tags(s) MASSGEDISNDDDDMHPAAAGMADGVHLLGFSDEILLHILSHVPSTDLILNVRRTCRKLAALCLDKSLIH TVLLQKDYQASEDKVRQLVKEIGREIQQLSMAGCYWLPGSTVEHVARCRSLVKVNLSGCHLTSLRLSKML SALQHLRSLAIDVSPGFDASQLSSECKATLSRVRELKQTLFTPSYGVVPCCTSLEKLLLYFEILDRTREG AILSGQLMVGQSNVPHYQNLRVFYARLAPGYINQEVVRLYLAVLSDRTPQNLHAFLISVPGSFAESGATK NLLDSMARNVVLDALQLPKSWLNGSSLLQHMKFNNPFYFSFSRCTLSGGHLIQQVINGGKDLRSLASLNL SGCVHCLSPDSLLRKAEDDIDSSILETLVASCCNLRHLNLSAAHHHSSEGLGRHLCQLLARLRHLRSLSL PVCSVADSAPRADRAPAQPAMHAVPRGFGKKVRVGVQSCPSPFSGQACPQPSSVFWSLLKNLPFLEHLEL IGSNFSSAMPRNEPAIRNSLPPCSRAQSVGDSEVAAIGQLAFLRHLTLAQLPSVLTGSGLVNIGLQCQQL RSLSLANLGMMGKVVYMPALSDMLKHCKRLRDLRLEQPYFSANAQFFQALSQCPSLQRLCLVSRSGTLQP DAVLAFMARCLQVVMCHLFTGESLATCKSLQQSLLRSFQAERPALNVVIFPLLHEGLTDVIRDVPLVHLD EITLFKSRVAEEPPNLWW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_024963 |
| ORF Size | 2154 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_024963.6 |
| RefSeq Size | 8253 bp |
| RefSeq ORF | 2157 bp |
| Locus ID | 80028 |
| Domains | F-box |
| Protein Families | Druggable Genome |
| MW | 78.7 kDa |
| Gene Summary | The protein encoded by this gene is a member of a family of proteins that contain an approximately 40-amino acid F-box motif. This motif is important for interaction with SKP1 and for targeting some proteins for degradation. The encoded protein has been shown to control the cellular level of FBXL7, a protein that induces mitotic arrest, by targeting it for polyubiquitylation and proteasomal degradation. Members of the F-box protein family, such as FBXL18, are characterized by an approximately 40-amino acid F-box motif. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains. [provided by RefSeq, Mar 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220401L3 | Lenti ORF clone of Human F-box and leucine-rich repeat protein 18 (FBXL18), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC220401L4 | Lenti ORF clone of Human F-box and leucine-rich repeat protein 18 (FBXL18), mGFP tagged |
CNY 7,700.00 |
|
| RG220401 | FBXL18 (tGFP-tagged) - Human F-box and leucine-rich repeat protein 18 (FBXL18) |
CNY 6,370.00 |
|
| SC318199 | FBXL18 (untagged)-Human F-box and leucine-rich repeat protein 18 (FBXL18) |
CNY 5,792.00 |
