Liver Carboxylesterase 1 (CES1) (NM_001025195) Human Tagged ORF Clone
CAT#: RC220445
- TrueORF®
CES1 (Myc-DDK-tagged)-Human carboxylesterase 1 (CES1), transcript variant 1
ORF Plasmid: tGFP
"NM_001025195" in other vectors (4)
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CNY 6,344.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | ACAT; CE-1; CEH; CES2; hCE-1; HMSE; HMSE1; PCE-1; REH; SES1; TGH |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC220445 representing NM_001025195
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGCTCCGTGCCTTTATCCTGGCCACTCTCTCTGCTTCCGCGGCTTGGGCAGGGCATCCGTCCTCGC CACCTGTGGTGGACACCGTGCATGGCAAAGTGCTGGGGAAGTTCGTCAGCTTAGAAGGATTTGCACAGCC TGTGGCCATTTTCCTGGGAATCCCTTTTGCCAAGCCGCCTCTTGGACCCCTGAGGTTTACTCCACCGCAG CCTGCAGAACCATGGAGCTTTGTGAAGAATGCCACCTCGTACCCTCCTATGTGCACCCAAGATCCCAAGG CGGGGCAGTTACTCTCAGAGCTATTTACAAACCGAAAGGAGAACATTCCTCTCAAGCTTTCTGAAGACTG TCTTTACCTCAATATTTACACTCCTGCTGACTTGACCAAGAAAAACAGGCTGCCGGTGATGGTGTGGATC CACGGAGGGGGGCTGATGGTGGGTGCGGCATCAACCTATGATGGGCTGGCCCTTGCTGCCCATGAAAACG TGGTGGTGGTGACCATTCAATATCGCCTGGGCATCTGGGGATTCTTCAGCACAGGGGATGAACACAGCCG GGGGAACTGGGGTCACCTGGACCAGGTGGCTGCCCTGCGCTGGGTCCAGGACAACATTGCCAGCTTTGGA GGGAACCCAGGCTCTGTGACCATCTTTGGAGAGTCAGCGGGAGGAGAAAGTGTCTCTGTTCTTGTTTTGT CTCCATTGGCCAAGAACCTCTTCCACCGGGCCATTTCTGAGAGTGGCGTGGCCCTCACTTCTGTTCTGGT GAAGAAAGGTGATGTCAAGCCCTTGGCTGAGCAAATTGCTATCACTGCTGGGTGCAAAACCACCACCTCT GCTGTCATGGTTCACTGCCTGCGACAGAAGACGGAAGAGGAGCTCTTGGAGACGACATTGAAAATGAAAT TCTTATCTCTGGACTTACAGGGAGACCCCAGAGAGAGTCAACCCCTTCTGGGCACTGTGATTGATGGGAT GCTGCTGCTGAAAACACCTGAAGAGCTTCAAGCTGAAAGGAATTTCCACACTGTCCCCTACATGGTCGGA ATTAACAAGCAGGAGTTTGGCTGGTTGATTCCAATGCAGTTGATGAGCTATCCACTCTCCGAAGGGCAAC TGGACCAGAAGACAGCCATGTCACTCCTGTGGAAGTCCTATCCCCTTGTTTGCATTGCTAAGGAACTGAT TCCAGAAGCCACTGAGAAATACTTAGGAGGAACAGACGACACTGTCAAAAAGAAAGACCTGTTCCTGGAC TTGATAGCAGATGTGATGTTTGGTGTCCCATCTGTGATTGTGGCCCGGAACCACAGAGATGCTGGAGCAC CCACCTACATGTATGAGTTTCAGTACCGTCCAAGCTTCTCATCAGACATGAAACCCAAGACGGTGATAGG AGACCACGGGGATGAGCTCTTCTCCGTCTTTGGGGCCCCATTTTTAAAAGAGGGTGCCTCAGAAGAGGAG ATCAGACTTAGCAAGATGGTGATGAAATTCTGGGCCAACTTTGCTCGCAATGGAAACCCCAATGGGGAAG GGCTGCCCCACTGGCCAGAGTACAACCAGAAGGAAGGGTATCTGCAGATTGGTGCCAACACCCAGGCGGC CCAGAAGCTGAAGGACAAAGAAGTAGCTTTCTGGACCAACCTCTTTGCCAAGAAGGCAGTGGAGAAGCCA CCCCAGACAGAACACATAGAGCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC220445 representing NM_001025195
Red=Cloning site Green=Tags(s) MWLRAFILATLSASAAWAGHPSSPPVVDTVHGKVLGKFVSLEGFAQPVAIFLGIPFAKPPLGPLRFTPPQ PAEPWSFVKNATSYPPMCTQDPKAGQLLSELFTNRKENIPLKLSEDCLYLNIYTPADLTKKNRLPVMVWI HGGGLMVGAASTYDGLALAAHENVVVVTIQYRLGIWGFFSTGDEHSRGNWGHLDQVAALRWVQDNIASFG GNPGSVTIFGESAGGESVSVLVLSPLAKNLFHRAISESGVALTSVLVKKGDVKPLAEQIAITAGCKTTTS AVMVHCLRQKTEEELLETTLKMKFLSLDLQGDPRESQPLLGTVIDGMLLLKTPEELQAERNFHTVPYMVG INKQEFGWLIPMQLMSYPLSEGQLDQKTAMSLLWKSYPLVCIAKELIPEATEKYLGGTDDTVKKKDLFLD LIADVMFGVPSVIVARNHRDAGAPTYMYEFQYRPSFSSDMKPKTVIGDHGDELFSVFGAPFLKEGASEEE IRLSKMVMKFWANFARNGNPNGEGLPHWPEYNQKEGYLQIGANTQAAQKLKDKEVAFWTNLFAKKAVEKP PQTEHIEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001025195 |
ORF Size | 1704 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001025195.2 |
RefSeq Size | 2027 bp |
RefSeq ORF | 1707 bp |
Locus ID | 1066 |
UniProt ID | P23141 |
Protein Families | Druggable Genome |
Protein Pathways | Drug metabolism - other enzymes |
MW | 62.59 kDa |
Gene Summary | This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This enzyme is the major liver enzyme and functions in liver drug clearance. Mutations of this gene cause carboxylesterase 1 deficiency. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jun 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC220445L3 | Lenti-ORF clone of CES1 (Myc-DDK-tagged)-Human carboxylesterase 1 (CES1), transcript variant 1 |
CNY 5,890.00 |
|
RC220445L4 | Lenti-ORF clone of CES1 (mGFP-tagged)-Human carboxylesterase 1 (CES1), transcript variant 1 |
CNY 5,890.00 |
|
RG220445 | CES1 (tGFP-tagged) - Human carboxylesterase 1 (CES1), transcript variant 1 |
CNY 7,944.00 |
|
SC302361 | CES1 (untagged)-Human carboxylesterase 1 (CES1), transcript variant 1 |
CNY 6,352.00 |
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