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CD272 (BTLA) (NM_001085357) Human Tagged ORF Clone
CAT＃: RC220628
BTLA (Myc-DDK-tagged)-Human B and T lymphocyte associated (BTLA), transcript variant 2
ORF Plasmid: tGFP
"NM_001085357" in other vectors (6)
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CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BTLA1; CD272 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC220628 representing NM_001085357
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGACATTGCCTGCCATGCTTGGAACTGGGAAATTATTTTGGGTCTTCTTCTTAATCCCATATCTGG ACATCTGGAACATCCATGGGAAAGAATCATGTGATGTACAGCTTTATATAAAGAGACAATCTGAACACTC CATCTTAGCAGGAGATCCCTTTGAACTAGAATGCCCTGTGAAATACTGTGCTAACAGGCCTCATGTGACT TGGTGCAAGCTCAATGGAACAACATGTGTAAAACTTGAAGATAGACAAACAAGTTGGAAGGAAGAGAAGA ACATTTCATTTTTCATTCTACATTTTGAACCAGTGCTTCCTAATGACAATGGGTCATACCGCTGTTCTGC AAATTTTCAGTCTAATCTCATTGAAAGCCACTCAACAACTCTTTATGTGACAGGAAAGCAAAATGAACTC TCTGACACAGCAGGAAGGGAAATTAACCTGGTTGATGCTCACCTTAAGAGTGAGCAAACAGAAGCAAGCA CCAGGCAAAATTCCCAAGTACTGCTATCAGAAACTGGAATTTATGATAATGACCCTGACCTTTGTTTCAG GATGCAGGAAGGGTCTGAAGTTTATTCTAATCCATGCCTGGAAGAAAACAAACCAGGCATTGTTTATGCT TCCCTGAACCATTCTGTCATTGGACTGAACTCAAGACTGGCAAGAAATGTAAAAGAAGCACCAACAGAAT ATGCATCCATATGTGTGAGGAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC220628 representing NM_001085357
Red=Cloning site Green=Tags(s) MKTLPAMLGTGKLFWVFFLIPYLDIWNIHGKESCDVQLYIKRQSEHSILAGDPFELECPVKYCANRPHVT WCKLNGTTCVKLEDRQTSWKEEKNISFFILHFEPVLPNDNGSYRCSANFQSNLIESHSTTLYVTGKQNEL SDTAGREINLVDAHLKSEQTEASTRQNSQVLLSETGIYDNDPDLCFRMQEGSEVYSNPCLEENKPGIVYA SLNHSVIGLNSRLARNVKEAPTEYASICVRS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001085357 |
| ORF Size | 723 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001085357.1, NP_001078826.1 |
| RefSeq Size | 3072 bp |
| RefSeq ORF | 726 bp |
| Locus ID | 151888 |
| UniProt ID | Q7Z6A9 |
| Protein Families | Transmembrane |
| MW | 27.1 kDa |
| Gene Summary | This gene encodes a member of the immunoglobulin superfamily. The encoded protein contains a single immunoglobulin (Ig) domain and is a receptor that relays inhibitory signals to suppress the immune response. Alternative splicing results in multiple transcript variants. Polymorphisms in this gene have been associated with an increased risk of rheumatoid arthritis. [provided by RefSeq, Aug 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220628L1 | Lenti ORF clone of Human B and T lymphocyte associated (BTLA), transcript variant 2, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC220628L2 | Lenti ORF clone of Human B and T lymphocyte associated (BTLA), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC220628L3 | Lenti ORF clone of Human B and T lymphocyte associated (BTLA), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC220628L4 | Lenti ORF clone of Human B and T lymphocyte associated (BTLA), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG220628 | BTLA (tGFP-tagged) - Human B and T lymphocyte associated (BTLA), transcript variant 2 |
CNY 4,370.00 |
|
| SC316167 | BTLA (untagged)-Human B and T lymphocyte associated (BTLA), transcript variant 2 |
CNY 3,990.00 |
