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SPO11 (NM_012444) Human Tagged ORF Clone
CAT＃: RC221227
SPO11 (Myc-DDK-tagged)-Human SPO11 meiotic protein covalently bound to DSB homolog (S. cerevisiae) (SPO11), transcript variant 1
ORF Plasmid: tGFP
"NM_012444" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CT35; SPATA43; TOPOVIA; TOPVIA |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC221227 representing NM_012444
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTTTGCACCTATGGGGCCCGAGGCCTCGTTCTTCGACGTTTTGGACCGACACAGGGAGTCCCTGC TGGCTGCCCTGAGGAGAGGTGGCAGGGAGCCCCCAACTGGGGGAAGCCGCCTGGCCTCCAGTTCTGAGGT TCTTGCATCTATAGAAAATATTATCCAAGACATAATCACAAGCTTGGCAAGAAATGAAGCACCTGCATTC ACGATAGACAACAGATCAAGCTGGGAAAACATAAAGTTTGAAGATTCTGTGGGTCTTCAGATGGTATCCC ATTGCACCACCAGAAAGATCAAAAGTGATTCACCAAAATCAGCTCAAAAATTTTCTCTAATCCTTAAAAT ATTGTCCATGATTTATAAATTAGTACAGAGCAACACTTATGCAACCAAAAGGGACATATATTACACTGAC AGTCAACTCTTTGGTAACCAGACTGTCGTCGACAATATTATCAATGACATTTCTTGCATGTTAAAAGTGT CAAGGAGGAGTCTACATATATTATCTACATCAAAAGGTTTAATTGCTGGCAACTTAAGATACATCGAGGA AGATGGCACCAAAGTGAATTGTACCTGTGGTGCAACGGCTGTTGCTGTGCCATCGAATATTCAAGGAATT CGGAATTTAGTTACAGATGCAAAGTTTGTATTAATTGTAGAAAAAGATGCAACATTTCAGCGGCTCCTAG ATGACAACTTTTGCAACAAATTGTCTCCTTGCATCATGATTACGGGAAAGGGAGTTCCTGATCTAAACAC AAGACTTTTAGTCAAGAAACTGTGGGATACATTTCATGTTCCTGTTTTCACTCTTGTAGATGCTGATCCA CATGGCATAGAAATAATGTGCATCTATAAGTATGGATCTATGTCTATGTCTTTTGAAGCTCATCATCTCA CAGTTCCAGCTATTAGATGGCTTGGTCTTCTCCCTTCTGATCTTAAAAGATTAAATGTACCTAAAGATAG TTTGATTCCACTGACAAAAAGGGACCAAATGAAACTTGACAGTATCCTGAGGAGACCTTATGTTACCTGC CAACCATTTTGGAGAAAAGAAATGGAAATAATGGCAGACTCTAAAATGAAGGCAGAAATTCAAGCTTTGA CTTTCCTATCATCAGATTATCTTTCCAGAGTGTACTTACCTAACAAATTAAAATTTGGAGGATGGATA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC221227 representing NM_012444
Red=Cloning site Green=Tags(s) MAFAPMGPEASFFDVLDRHRESLLAALRRGGREPPTGGSRLASSSEVLASIENIIQDIITSLARNEAPAF TIDNRSSWENIKFEDSVGLQMVSHCTTRKIKSDSPKSAQKFSLILKILSMIYKLVQSNTYATKRDIYYTD SQLFGNQTVVDNIINDISCMLKVSRRSLHILSTSKGLIAGNLRYIEEDGTKVNCTCGATAVAVPSNIQGI RNLVTDAKFVLIVEKDATFQRLLDDNFCNKLSPCIMITGKGVPDLNTRLLVKKLWDTFHVPVFTLVDADP HGIEIMCIYKYGSMSMSFEAHHLTVPAIRWLGLLPSDLKRLNVPKDSLIPLTKRDQMKLDSILRRPYVTC QPFWRKEMEIMADSKMKAEIQALTFLSSDYLSRVYLPNKLKFGGWI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012444 |
| ORF Size | 1188 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_012444.3 |
| RefSeq Size | 1826 bp |
| RefSeq ORF | 1191 bp |
| Locus ID | 23626 |
| UniProt ID | Q9Y5K1 |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 44.4 kDa |
| Gene Summary | Meiotic recombination and chromosome segregation require the formation of double-strand breaks (DSBs) in paired chromosome homologs. During meiosis in yeast, a meiotic recombination protein is covalently-linked to the 5' end of DSBs and is essential for the formation of DSBs. The protein encoded by this gene is similar in sequence and conserved features to the yeast meiotic recombination protein. The encoded protein belongs to the TOP6A protein family. Several transcript variants encoding different isoforms have been found for this gene, but the full-length nature of only two of them have been described. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Centromeres are dismantled by foundational meiotic proteins Spo11 and Rec8
,Hou, H;Kyriacou, E;Thadani, R;Klutstein, M;Chapman, JH;Cooper, JP;,
Nature
,PubMed ID 33658710
[SPO11]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221227L1 | Lenti ORF clone of Human SPO11 meiotic protein covalently bound to DSB homolog (S. cerevisiae) (SPO11), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC221227L2 | Lenti ORF clone of Human SPO11 meiotic protein covalently bound to DSB homolog (S. cerevisiae) (SPO11), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC221227L3 | Lenti ORF clone of Human SPO11 meiotic protein covalently bound to DSB homolog (S. cerevisiae) (SPO11), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC221227L4 | Lenti ORF clone of Human SPO11 meiotic protein covalently bound to DSB homolog (S. cerevisiae) (SPO11), transcript variant 1, mGFP tagged |
CNY 6,056.00 |
|
| RG221227 | SPO11 (tGFP-tagged) - Human SPO11 meiotic protein covalently bound to DSB homolog (S. cerevisiae) (SPO11), transcript variant 1 |
CNY 4,370.00 |
|
| SC303983 | SPO11 (untagged)-Human SPO11 meiotic protein covalently bound to DSB homolog (S. cerevisiae) (SPO11), transcript variant 1 |
CNY 3,656.00 |
