FCHSD2 (NM_014824) Human Tagged Lenti ORF Clone
CAT#: RC221241L1
- LentiORF®
Lenti ORF clone of Human FCH and double SH3 domains 2 (FCHSD2), Myc-DDK-tagged
"NM_014824" in other vectors (7)
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CNY 9,600.00
CNY 600.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | NWK; NWK1; SH3MD3 |
Vector | pLenti-C-Myc-DDK |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC221241).
|
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_014824 |
ORF Size | 2052 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_014824.1, NP_055639.1 |
RefSeq Size | 4341 bp |
RefSeq ORF | 2223 bp |
Locus ID | 9873 |
UniProt ID | O94868 |
Domains | SH3 |
MW | 77.6 kDa |
Gene Summary | Adapter protein that plays a role in endocytosis via clathrin-coated pits. Contributes to the internalization of cell surface receptors, such as integrin ITGB1 and transferrin receptor (PubMed:29887380). Promotes endocytosis of EGFR in cancer cells, and thereby contributes to the down-regulation of EGFR signaling (PubMed:30249660). Recruited to clathrin-coated pits during a mid-to-late stage of assembly, where it is required for normal progress from U-shaped intermediate stage pits to terminal, omega-shaped pits (PubMed:29887380). Binds to membranes enriched in phosphatidylinositol 3,4-bisphosphate or phosphatidylinositol 3,4,5-trisphosphate (PubMed:29887380). When bound to membranes, promotes actin polymerization via its interaction with WAS and/or WASL which leads to the activation of the Arp2/3 complex. Does not promote actin polymerisation in the absence of membranes (PubMed:29887380).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC221241 | FCHSD2 (Myc-DDK-tagged)-Human FCH and double SH3 domains 2 (FCHSD2) |
CNY 7,512.00 |
|
RC221241L2 | Lenti ORF clone of Human FCH and double SH3 domains 2 (FCHSD2), mGFP tagged |
CNY 9,912.00 |
|
RC221241L3 | Lenti ORF clone of Human FCH and double SH3 domains 2 (FCHSD2), Myc-DDK-tagged |
CNY 9,600.00 |
|
RC221241L4 | Lenti ORF clone of Human FCH and double SH3 domains 2 (FCHSD2), mGFP tagged |
CNY 9,600.00 |
|
RG221241 | FCHSD2 (tGFP-tagged) - Human FCH and double SH3 domains 2 (FCHSD2) |
CNY 9,112.00 |
|
SC108752 | FCHSD2 (untagged)-Human FCH and double SH3 domains 2 (FCHSD2) |
CNY 7,528.00 |
|
SC327995 | FCHSD2 (untagged)-Human FCH and double SH3 domains 2 (FCHSD2) |
CNY 14,160.00 |