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Tryptophan Hydroxylase (TPH1) (NM_004179) Human Tagged ORF Clone
CAT＃: RC221254
TPH1 (Myc-DDK-tagged)-Human tryptophan hydroxylase 1 (TPH1)
ORF Plasmid: tGFP
"NM_004179" in other vectors (6)
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CNY 5,488.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | TPRH; TRPH |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC221254 representing NM_004179
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTGAAGACAATAAGGAGAACAAAGACCATTCCTTAGAAAGGGGAAGAGCAAGTCTCATTTTTTCCT TAAAGAATGAAGTTGGAGGACTTATAAAAGCCCTGAAAATCTTTCAGGAGAAGCATGTGAATCTGTTACA TATCGAGTCCCGAAAATCAAAAAGAAGAAACTCAGAATTTGAGATTTTTGTTGACTGTGACATCAACAGA GAACAATTGAATGATATTTTTCATCTGCTGAAGTCTCATACCAATGTTCTCTCTGTGAATCTACCAGATA ATTTTACTTTGAAGGAAGATGGTATGGAAACTGTTCCTTGGTTTCCAAAGAAGATTTCTGACCTGGACCA TTGTGCCAACAGAGTTCTGATGTATGGATCTGAACTAGATGCAGACCATCCTGGCTTCAAAGACAATGTC TACCGTAAACGTCGAAAGTATTTTGCGGACTTGGCTATGAACTATAAACATGGAGACCCCATTCCAAAGG TTGAATTCACTGAAGAGGAGATTAAGACCTGGGGAACCGTATTCCAAGAGCTCAACAAACTCTACCCAAC CCATGCTTGCAGAGAGTATCTCAAAAACTTACCTTTGCTTTCTAAATATTGTGGATATCGGGAGGATAAT ATCCCACAATTGGAAGATGTCTCCAACTTTTTAAAAGAGCGTACAGGTTTTTCCATCCGTCCTGTGGCTG GTTACTTATCACCAAGAGATTTCTTATCAGGTTTAGCCTTTCGAGTTTTTCACTGCACTCAATATGTGAG ACACAGTTCAGATCCCTTCTATACCCCAGAGCCAGATACCTGCCATGAACTCTTAGGTCATGTCCCGCTT TTGGCTGAACCTAGTTTTGCCCAATTCTCCCAAGAAATTGGCTTGGCTTCTCTTGGCGCTTCAGAGGAGG CTGTTCAAAAACTGGCAACGTGCTACTTTTTCACTGTGGAGTTTGGTCTATGTAAACAAGATGGACAGCT AAGAGTCTTTGGTGCTGGCTTACTTTCTTCTATCAGTGAACTCAAACATGCACTTTCTGGACATGCCAAA GTAAAGCCCTTTGATCCCAAGATTACCTGCAAACAGGAATGTCTTATCACAACTTTTCAAGATGTCTACT TTGTATCTGAAAGTTTTGAAGATGCAAAGGAGAAGATGAGAGAATTTACCAAAACAATTAAGCGTCCATT TGGAGTGAAGTATAATCCATATACACGGAGTATTCAGATCCTGAAAGACACCAAGAGCATAACCAGTGCC ATGAATGAGCTGCAGCATGATCTCGATGTTGTCAGTGATGCCCTTGCTAAGGTCAGCAGGAAGCCGAGTA TC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC221254 representing NM_004179
Red=Cloning site Green=Tags(s) MIEDNKENKDHSLERGRASLIFSLKNEVGGLIKALKIFQEKHVNLLHIESRKSKRRNSEFEIFVDCDINR EQLNDIFHLLKSHTNVLSVNLPDNFTLKEDGMETVPWFPKKISDLDHCANRVLMYGSELDADHPGFKDNV YRKRRKYFADLAMNYKHGDPIPKVEFTEEEIKTWGTVFQELNKLYPTHACREYLKNLPLLSKYCGYREDN IPQLEDVSNFLKERTGFSIRPVAGYLSPRDFLSGLAFRVFHCTQYVRHSSDPFYTPEPDTCHELLGHVPL LAEPSFAQFSQEIGLASLGASEEAVQKLATCYFFTVEFGLCKQDGQLRVFGAGLLSSISELKHALSGHAK VKPFDPKITCKQECLITTFQDVYFVSESFEDAKEKMREFTKTIKRPFGVKYNPYTRSIQILKDTKSITSA MNELQHDLDVVSDALAKVSRKPSI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004179 |
| ORF Size | 1332 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004179.3 |
| RefSeq Size | 1335 bp |
| RefSeq ORF | 1335 bp |
| Locus ID | 7166 |
| UniProt ID | P17752 |
| Domains | biopterin_H, ACT |
| Protein Families | Druggable Genome |
| Protein Pathways | Metabolic pathways, Tryptophan metabolism |
| MW | 50.8 kDa |
| Gene Summary | This gene encodes a member of the aromatic amino acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. Mutations in this gene have been associated with an elevated risk for a variety of diseases and disorders, including schizophrenia, somatic anxiety, anger-related traits, bipolar disorder, suicidal behavior, addictions, and others.[provided by RefSeq, Apr 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221254L1 | Lenti ORF clone of Human tryptophan hydroxylase 1 (TPH1), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC221254L2 | Lenti ORF clone of Human tryptophan hydroxylase 1 (TPH1), mGFP tagged |
CNY 5,890.00 |
|
| RC221254L3 | Lenti ORF clone of Human tryptophan hydroxylase 1 (TPH1), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC221254L4 | Lenti ORF clone of Human tryptophan hydroxylase 1 (TPH1), mGFP tagged |
CNY 7,888.00 |
|
| RG221254 | TPH1 (tGFP-tagged) - Human tryptophan hydroxylase 1 (TPH1) |
CNY 7,088.00 |
|
| SC108521 | TPH1 (untagged)-Human tryptophan hydroxylase 1 (TPH1) |
CNY 5,488.00 |
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