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ATP8A2 (NM_016529) Human Tagged ORF Clone
CAT＃: RC221445
- TrueORF®
ATP8A2 (Myc-DDK-tagged)-Human ATPase, aminophospholipid transporter, class I, type 8A, member 2 (ATP8A2)
ORF Plasmid: tGFP
"NM_016529" in other vectors (5)
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CNY 11,880.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ATP; ATPIB; CAMRQ4; IB; ML-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC221445 representing NM_016529
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGAACGGCGCAGGCCTGGACAAAGCTCTTAAGATGTCCCTGCCGCGGAGGTCGAGGATCCGCTCGT CCGTGGGACCTGTTCGTTCTTCTTTGGGCTATAAGAAGGCAGAGGATGAGATGTCCCGGGCCACGTCTGT TGGAGACCAGCTGGAGGCACCCGCCCGCACCATTTACCTCAACCAACCGCATCTCAACAAATTCCGCGAC AACCAGATCAGTACGGCCAAGTACAGCGTGTTGACATTTCTACCTCGATTCTTGTATGAGCAGATTAGAA GAGCTGCTAATGCCTTCTTTCTCTTCATTGCCTTATTACAGCAAATTCCAGATGTATCTCCAACAGGAAG ATATACCACCCTGGTGCCATTGATCATTATTTTAACAATTGCAGGCATCAAAGAGATTGTAGAAGATTTT AAGCGACACAAGGCAGACAATGCAGTTAACAAAAAGAAAACAATAGTGTTAAGAAATGGTATGTGGCATA CCATTATGTGGAAAGAGGTGGCAGTGGGAGACATTGTGAAGGTCGTCAATGGGCAGTATCTTCCAGCAGA TGTGGTCCTGCTGTCATCCAGTGAACCTCAGGCAATGTGTTATGTTGAAACAGCTAATCTGGATGGGGAG ACGAACCTTAAAATACGTCAGGGTTTGAGTCACACTGCTGACATGCAAACACGTGAAGTTCTGATGAAGT TATCTGGAACTATAGAGTGTGAAGGGCCCAACCGCCACCTCTATGACTTCACTGGAAACTTGAACTTAGA TGGGAAAAGCCTTGTTGCCCTTGGGCCTGACCAGATCTTATTAAGAGGTACACAGCTTAGAAATACTCAG TGGGTCTTTGGCATAGTTGTTTATACTGGACACGACACCAAACTCATGCAGAATTCAACCAAAGCGCCTC TCAAGAGATCAAATGTTGAGAAGGTGACTAACGTGCAGATCCTGGTGTTGTTTGGCATCCTCTTGGTCAT GGCCTTGGTGAGCTCGGCGGGGGCCCTGTACTGGAACAGGTCTCATGGTGAAAAGAACTGGTACATCAAG AAGATGGACACCACCTCAGATAATTTTGGATACAACCTACTGACGTTCATCATCTTATACAACAATCTTA TTCCCATCAGTCTGTTGGTGACTCTTGAGGTTGTGAAGTATACTCAAGCCCTTTTCATAAACTGGGACAC AGATATGTATTATATAGGAAATGACACTCCTGCCATGGCCAGGACATCAAACCTTAATGAAGAGCTTGGG CAGGTGAAATATCTCTTTTCTGACAAGACTGGAACGCTTACATGCAATATCATGAACTTTAAGAAGTGCA GCATTGCCGGAGTAACCTATGGTCACTTCCCAGAATTGGCAAGAGAGCCGTCTTCAGATGACTTCTGTCG GATGCCTCCTCCCTGTAGTGATTCCTGTGACTTTGATGACCCCAGGCTGTTGAAGAACATTGAGGATCGC CATCCCACAGCCCCTTGCATTCAGGAGTTCCTCACCCTTCTGGCCGTGTGCCACACGGTTGTTCCTGAGA AGGATGGAGATAACATCATCTACCAGGCCTCTTCCCCAGATGAAGCTGCTTTGGTGAAAGGAGCTAAAAA GCTGGGCTTTGTCTTCACAGCCAGAACACCATTCTCAGTCATCATAGAAGCGATGGGACAGGAACAAACA TTCGGAATCCTTAATGTCCTGGAATTTTCTAGTGACAGAAAAAGAATGTCTGTAATTGTTCGAACTCCTT CAGGACGACTTCGGCTTTACTGTAAAGGGGCTGATAATGTGATTTTTGAGAGACTTTCAAAAGACTCAAA ATATATGGAGGAAACATTATGCCATCTGGAATACTTTGCCACGGAAGGCTTGCGGACTCTCTGTGTGGCT TATGCTGATCTCTCTGAGAATGAGTATGAGGAGTGGCTGAAAGTCTATCAGGAAGCCAGCACCATATTGA AGGACAGAGCTCAACGGTTGGAAGAGTGTTACGAGATCATTGAGAAGAATTTGCTGCTACTTGGAGCCAC AGCCATAGAAGATCGCCTTCAAGCAGGAGTTCCAGAAACCATCGCAACACTGTTGAAGGCAGAAATTAAA ATATGGGTGTTGACAGGAGACAAACAAGAAACTGCGATTAATATAGGGTATTCCTGCCGATTGGTATCGC AGAATATGGCCCTTATCCTATTGAAGGAGGACTCTTTGGATGCCACAAGGGCAGCCATTACTCAGCACTG CACTGACCTTGGGAATTTGCTGGGCAAGGAAAATGACGTGGCCCTGATCATCGATGGCCACACCCTGAAG TACGCGCTCTCCTTCGAAGTCCGGAGGAGTTTCCTGGATTTGGCACTCTCGTGCAAAGCGGTCATATGCT GCAGAGTGTCTCCTCTGCAGAAGTCTGAGATAGTGGATGTGGTGAAGAAGCGGGTGAAGGCCATCACCCT CGCCATCGGAGACGGCGCCAACGATGTCGGGATGATCCAGACAGCCCACGTGGGTGTGGGAATCAGTGGG AATGAAGGCATGCAGGCCACCAACAACTCGGATTACGCCATCGCACAGTTTTCCTACTTAGAGAAGCTTC TGTTGGTTCATGGAGCCTGGAGCTACAACCGGGTGACCAAGTGCATCTTGTACTGCTTCTATAAGAACGT GGTCCTGTATATTATTGAGCTTTGGTTCGCCTTTGTTAATGGATTTTCTGGGCAGATTTTATTTGAACGT TGGTGCATCGGCCTGTACAATGTGATTTTCACCGCTTTGCCGCCCTTCACTCTGGGAATCTTTGAGAGGT CTTGCACTCAGGAGAGCATGCTCAGGTTTCCCCAGCTCTACAAAATCACCCAGAATGGCGAAGGCTTCAA CACAAAGGTTTTCTGGGGTCACTGCATCAACGCCTTGGTCCACTCCCTCATCCTCTTCTGGTTTCCCATG AAAGCTCTGGAGCATGATACTGTGTTGACAAGTGGTCATGCTACCGACTATTTATTTGTTGGAAATATTG TTTACACATATGTTGTTGTTACTGTTTGTCTGAAAGCTGGTTTGGAGACCACAGCTTGGACTAAATTCAG TCATCTGGCTGTCTGGGGAAGCATGCTGACCTGGCTGGTGTTTTTTGGCATCTACTCGACCATCTGGCCC ACCATTCCCATTGCTCCAGATATGAGAGGACAGGCAACTATGGTCCTGAGCTCCGCACACTTCTGGTTGG GATTATTTCTGGTTCCTACTGCCTGTTTGATTGAAGATGTGGCATGGAGAGCAGCCAAGCACACCTGCAA AAAGACATTGCTGGAGGAGGTGCAGGAGCTGGAAACCAAGTCTCGAGTCCTGGGAAAAGCGGTGCTGCGG GATAGCAATGGAAAGAGGCTGAACGAGCGCGACCGCCTGATCAAGAGGCTGGGCCGGAAGACGCCCCCGA CGCTGTTCCGGGGCAGCTCCCTGCAGCAGGGCGTCCCGCATGGGTATGCTTTTTCTCAAGAAGAACACGG AGCTGTTAGTCAGGAAGAAGTCATCCGTGCTTATGACACCACCAAAAAGAAATCCAGGAAGAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC221445 representing NM_016529
Red=Cloning site Green=Tags(s) MLNGAGLDKALKMSLPRRSRIRSSVGPVRSSLGYKKAEDEMSRATSVGDQLEAPARTIYLNQPHLNKFRD NQISTAKYSVLTFLPRFLYEQIRRAANAFFLFIALLQQIPDVSPTGRYTTLVPLIIILTIAGIKEIVEDF KRHKADNAVNKKKTIVLRNGMWHTIMWKEVAVGDIVKVVNGQYLPADVVLLSSSEPQAMCYVETANLDGE TNLKIRQGLSHTADMQTREVLMKLSGTIECEGPNRHLYDFTGNLNLDGKSLVALGPDQILLRGTQLRNTQ WVFGIVVYTGHDTKLMQNSTKAPLKRSNVEKVTNVQILVLFGILLVMALVSSAGALYWNRSHGEKNWYIK KMDTTSDNFGYNLLTFIILYNNLIPISLLVTLEVVKYTQALFINWDTDMYYIGNDTPAMARTSNLNEELG QVKYLFSDKTGTLTCNIMNFKKCSIAGVTYGHFPELAREPSSDDFCRMPPPCSDSCDFDDPRLLKNIEDR HPTAPCIQEFLTLLAVCHTVVPEKDGDNIIYQASSPDEAALVKGAKKLGFVFTARTPFSVIIEAMGQEQT FGILNVLEFSSDRKRMSVIVRTPSGRLRLYCKGADNVIFERLSKDSKYMEETLCHLEYFATEGLRTLCVA YADLSENEYEEWLKVYQEASTILKDRAQRLEECYEIIEKNLLLLGATAIEDRLQAGVPETIATLLKAEIK IWVLTGDKQETAINIGYSCRLVSQNMALILLKEDSLDATRAAITQHCTDLGNLLGKENDVALIIDGHTLK YALSFEVRRSFLDLALSCKAVICCRVSPLQKSEIVDVVKKRVKAITLAIGDGANDVGMIQTAHVGVGISG NEGMQATNNSDYAIAQFSYLEKLLLVHGAWSYNRVTKCILYCFYKNVVLYIIELWFAFVNGFSGQILFER WCIGLYNVIFTALPPFTLGIFERSCTQESMLRFPQLYKITQNGEGFNTKVFWGHCINALVHSLILFWFPM KALEHDTVLTSGHATDYLFVGNIVYTYVVVTVCLKAGLETTAWTKFSHLAVWGSMLTWLVFFGIYSTIWP TIPIAPDMRGQATMVLSSAHFWLGLFLVPTACLIEDVAWRAAKHTCKKTLLEEVQELETKSRVLGKAVLR DSNGKRLNERDRLIKRLGRKTPPTLFRGSSLQQGVPHGYAFSQEEHGAVSQEEVIRAYDTTKKKSRKK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016529 |
| ORF Size | 3564 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_016529.6 |
| RefSeq Size | 5006 bp |
| RefSeq ORF | 3567 bp |
| Locus ID | 51761 |
| UniProt ID | Q9NTI2 |
| Protein Families | Transmembrane |
| MW | 133.4 kDa |
| Gene Summary | The protein encoded by this gene is a member of the P4 ATPase family of proteins, which are thought to be involved in a process called lipid flipping, whereby phospholipids are translocated inwards from the exoplasmic leaflet to the cytosolic leaflet of the cell membrane, which aids in generating and maintaining asymmetry in membrane lipids. This protein is predicted to contain an E1 E2 ATPase, a haloacid dehalogenase-like hydrolase (HAD) domain, and multiple transmembrane domains. Associations between this protein and cell cycle control protein 50A are important for translocation of phosphatidylserine across membranes. Mutations in this gene have been associated with a syndrome (CAMRQ4) characterized by cerebellar ataxia and cognitive disabilities. In addition, a translocation breakpoint within this gene was observed in an individual with neurological dysfunction. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221445L3 | Lenti-ORF clone of ATP8A2 (Myc-DDK-tagged)-Human ATPase, aminophospholipid transporter, class I, type 8A, member 2 (ATP8A2) |
CNY 13,780.00 |
|
| RC221445L4 | Lenti-ORF clone of ATP8A2 (mGFP-tagged)-Human ATPase, aminophospholipid transporter, class I, type 8A, member 2 (ATP8A2) |
CNY 13,780.00 |
|
| RG221445 | ATP8A2 (tGFP-tagged) - Human ATPase, aminophospholipid transporter, class I, type 8A, member 2 (ATP8A2) |
CNY 13,110.00 |
|
| SC114195 | ATP8A2 (untagged)-Human ATPase, aminophospholipid transporter, class I, type 8A, member 2 (ATP8A2) |
CNY 4,576.00 |
|
| SC318540 | ATP8A2 (untagged)-Human ATPase, aminophospholipid transporter, class I, type 8A, member 2 (ATP8A2) |
CNY 22,800.00 |
