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MYO1B (NM_012223) Human Tagged ORF Clone
CAT＃: RC221689
- TrueORF®
MYO1B (Myc-DDK-tagged)-Human myosin IB (MYO1B), transcript variant 2
ORF Plasmid: tGFP
"NM_012223" in other vectors (4)
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CNY 7,504.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MMI-alpha; MMIa; MYH-1c; myr1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC221689 representing NM_012223
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAAAATGGAGGTGAAAACCTCACTTCTGGACAATATGATTGGAGTTGGGGATATGGTTCTTTTAG AACCTCTCAATGAGGAGACCTTCATCAACAACCTCAAGAAGCGCTTTGACCACAGTGAAATATACACATA CATTGGAAGTGTGGTTATATCTGTTAACCCATACCGGTCTTTACCCATTTATTCACCAGAGAAAGTGGAA GAATACAGGAACAGAAATTTTTATGAACTGAGCCCTCACATCTTTGCCCTTTCGGATGAAGCATACAGAT CCCTACGAGATCAAGATAAGGACCAATGTATTCTCATTACTGGGGAAAGTGGAGCAGGAAAAACAGAGGC CAGTAAGCTTGTCATGTCCTATGTGGCAGCTGTTTGTGGAAAAGGAGCAGAAGTTAATCAAGTTAAAGAA CAGCTTTTACAGTCCAACCCGGTCCTGGAAGCTTTTGGAAATGCCAAAACTGTAAGGAATGACAACTCCT CTAGATTTGGCAAATATATGGATATTGAATTTGACTTTAAAGGCGATCCACTAGGAGGAGTAATAAGTAA CTATCTTTTAGAGAAATCTCGGGTTGTTAAACAGCCAAGAGGTGAAAGAAACTTCCATGTGTTCTATCAG CTGCTCTCTGGTGCCTCTGAAGAGCTCCTCAATAAACTTAAGCTTGAGAGGGATTTCAGCAGGTATAACT ACCTGAGTCTGGATTCGGCCAAAGTGAATGGAGTGGATGATGCAGCAAATTTTAGAACCGTGCGGAATGC CATGCAGATTGTGGGCTTTATGGATCATGAAGCTGAGTCTGTCTTGGCGGTGGTGGCAGCAGTGTTGAAA CTGGGGAACATTGAGTTCAAGCCCGAATCTCGAGTGAATGGTCTAGATGAAAGCAAAATCAAAGATAAAA ATGAGTTAAAAGAAATTTGTGAATTGACCGGCATTGATCAATCAGTTCTAGAACGAGCATTCAGTTTCCG AACAGTTGAGGCCAAACAGGAGAAAGTTTCAACTACACTGAATGTGGCTCAGGCTTATTATGCCCGTGAT GCTCTGGCTAAAAACCTCTACAGCAGGTTGTTTTCATGGTTGGTAAATCGAATCAATGAAAGCATTAAGG CACAAACAAAAGTGAGAAAGAAGGTCATGGGTGTTCTGGACATTTATGGCTTTGAGATTTTCGAGGACAA CAGCTTTGAGCAGTTCATTATTAATTATTGTAACGAAAAGCTGCAACAAATCTTCATTGAACTTACTCTT AAAGAAGAGCAGGAGGAGTATATACGGGAGGATATAGAATGGACTCACATTGACTACTTCAATAATGCTA TCATTTGTGACCTAATAGAAAATAACACAAATGGAATCCTGGCCATGCTGGATGAAGAGTGCCTCAGACC TGGCACAGTCACTGATGAGACCTTCTTAGAAAAGCTGAACCAAGTATGTGCCACCCACCAGCATTTTGAG AGCAGGATGAGCAAGTGCTCTCGGTTCCTCAATGACACGTCTCTGCCTCACAGCTGCTTCAGGATCCAGC ATTATGCTGGAAAGGTGCTGTACCAGGTGGAAGGATTCGTTGACAAAAACAATGACCTTCTCTATCGAGA CCTGTCCCAAGCCATGTGGAAGGCCAGCCATGCCCTCATCAAGTCTTTGTTCCCCGAAGGGAATCCCGCC AAGATCAACCTGAAAAGGCCTCCTACAGCAGGCTCACAGTTCAAGGCATCCGTGGCCACTCTGATGAAAA ACCTACAGACCAAGAACCCAAACTATATTAGGTGTATCAAACCGAATGATAAAAAAGCAGCACACATCTT CAACGAGGCTCTAGTGTGTCATCAGATCAGGTACCTGGGGCTTTTGGAGAACGTCCGAGTGCGGAGGGCA GGCTACGCCTTCAGGCAGGCCTATGAACCTTGCCTAGAAAGATACAAAATGCTTTGTAAACAAACATGGC CTCATTGGAAAGGACCAGCCAGGTCTGGTGTGGAGGTCCTATTTAATGAATTAGAAATTCCCGTGGAAGA ATACTCCTTTGGTAGATCAAAGATATTCATCCGAAACCCAAGAACATTATTCAAATTAGAAGACCTGAGG AAGCAACGCCTGGAGGACTTGGCCACTCTCATTCAGAAGATATATCGGGGGTGGAAATGCCGCACACACT TCCTGCTAATGAAAAAAAGCCAAATTGTGATTGCCGCCTGGTACAGGAGATATGCGCAACAAAAGAGGTA CCAGCAGACAAAGAGTTCCGCCTTAGTAATTCAGTCTTATATCCGGGGTTGGAAGGCTCGAAAAATTCTG CGGGAACTGAAGCATCAAAAGCGCTGTAAGGAAGCAGTCACGACCATTGCTGCATATTGGCATGGGACCC AGGTACGTAGAGAATACAGGAAATTCTTCAGAGCCAATGCTGGAAAGAAAATCTATGAGTTTACGCTTCA GAGAATTGTGCAAAAATACTTCTTGGAAATGAAAAATAAGATGCCTTCCTTATCTCCAATAGACAAGAAT TGGCCCTCAAGACCTTACTTATTCTTGGATTCTACTCACAAGGAGCTAAAAAGGATTTTCCACTTGTGGA GGTGTAAAAAATACAGGGACCAATTCACAGACCAGCAGAAACTTATTTATGAAGAGAAACTAGAAGCCAG TGAACTCTTCAAAGACAAGAAGGCTTTATACCCATCTAGTGTTGGGCAACCATTCCAAGGGGCTTACCTG GAAATCAACAAGAACCCCAAGTATAAGAAACTCAAAGATGCCATTGAAGAAAAGATCATCATTGCTGAAG TCGTGAACAAAATTAACCGTGCTAATGGGAAGAGTACATCTCGGATTTTCCTCTTAACAAACAATAATCT CCTTCTTGCTGACCAAAAGTCTGGACAAATCAAGTCAGAGGTTCCATTGGTGGATGTGACCAAGGTATCA ATGAGCTCACAAAATGATGGCTTCTTCGCCGTCCACCTCAAAGAGGGCTCAGAAGCAGCTAGTAAAGGAG ACTTTCTCTTCAGCAGTGATCACCTGATTGAAATGGCCACCAAGCTCTATCGCACAACTCTCAGCCAAAC CAAACAGAAGCTCAATATTGAGATTTCCGATGAGTTCCTGGTACAGTTCAGACAGGACAAAGTATGTGTG AAGTTTATTCAGGGAAACCAGAAAAATGGGAGTGTCCCAACATGTAAACGAAAAAACAACCGTCTCCTTG AAGTTGCTGTCCCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC221689 representing NM_012223
Red=Cloning site Green=Tags(s) MAKMEVKTSLLDNMIGVGDMVLLEPLNEETFINNLKKRFDHSEIYTYIGSVVISVNPYRSLPIYSPEKVE EYRNRNFYELSPHIFALSDEAYRSLRDQDKDQCILITGESGAGKTEASKLVMSYVAAVCGKGAEVNQVKE QLLQSNPVLEAFGNAKTVRNDNSSRFGKYMDIEFDFKGDPLGGVISNYLLEKSRVVKQPRGERNFHVFYQ LLSGASEELLNKLKLERDFSRYNYLSLDSAKVNGVDDAANFRTVRNAMQIVGFMDHEAESVLAVVAAVLK LGNIEFKPESRVNGLDESKIKDKNELKEICELTGIDQSVLERAFSFRTVEAKQEKVSTTLNVAQAYYARD ALAKNLYSRLFSWLVNRINESIKAQTKVRKKVMGVLDIYGFEIFEDNSFEQFIINYCNEKLQQIFIELTL KEEQEEYIREDIEWTHIDYFNNAIICDLIENNTNGILAMLDEECLRPGTVTDETFLEKLNQVCATHQHFE SRMSKCSRFLNDTSLPHSCFRIQHYAGKVLYQVEGFVDKNNDLLYRDLSQAMWKASHALIKSLFPEGNPA KINLKRPPTAGSQFKASVATLMKNLQTKNPNYIRCIKPNDKKAAHIFNEALVCHQIRYLGLLENVRVRRA GYAFRQAYEPCLERYKMLCKQTWPHWKGPARSGVEVLFNELEIPVEEYSFGRSKIFIRNPRTLFKLEDLR KQRLEDLATLIQKIYRGWKCRTHFLLMKKSQIVIAAWYRRYAQQKRYQQTKSSALVIQSYIRGWKARKIL RELKHQKRCKEAVTTIAAYWHGTQVRREYRKFFRANAGKKIYEFTLQRIVQKYFLEMKNKMPSLSPIDKN WPSRPYLFLDSTHKELKRIFHLWRCKKYRDQFTDQQKLIYEEKLEASELFKDKKALYPSSVGQPFQGAYL EINKNPKYKKLKDAIEEKIIIAEVVNKINRANGKSTSRIFLLTNNNLLLADQKSGQIKSEVPLVDVTKVS MSSQNDGFFAVHLKEGSEAASKGDFLFSSDHLIEMATKLYRTTLSQTKQKLNIEISDEFLVQFRQDKVCV KFIQGNQKNGSVPTCKRKNNRLLEVAVP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012223 |
| ORF Size | 3234 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_012223.5 |
| RefSeq Size | 4825 bp |
| RefSeq ORF | 3237 bp |
| Locus ID | 4430 |
| UniProt ID | O43795 |
| Domains | myosin_head |
| MW | 124.8 kDa |
| Gene Summary | Motor protein that may participate in process critical to neuronal development and function such as cell migration, neurite outgrowth and vesicular transport.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221689L3 | Lenti ORF clone of Human myosin IB (MYO1B), transcript variant 2, Myc-DDK-tagged |
CNY 12,640.00 |
|
| RC221689L4 | Lenti ORF clone of Human myosin IB (MYO1B), transcript variant 2, mGFP tagged |
CNY 12,640.00 |
|
| RG221689 | MYO1B (tGFP-tagged) - Human myosin IB (MYO1B), transcript variant 2 |
CNY 11,780.00 |
|
| SC303943 | MYO1B (untagged)-Human myosin IB (MYO1B), transcript variant 2 |
CNY 7,512.00 |
