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NFX1 (NM_147134) Human Tagged ORF Clone
CAT＃: RC221810
- TrueORF®
NFX1 (Myc-DDK-tagged)-Human nuclear transcription factor, X-box binding 1 (NFX1), transcript variant 3
ORF Plasmid: tGFP
"NM_147134" in other vectors (4)
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CNY 8,740.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | NFX2; TEG-42; Tex42 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC221810 representing NM_147134
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGGCGCCTCCTGTCTCAGGTACTTTTAAATTCAATACAGATGCTGCTGAATTCATTCCTCAGG AGAAAAAAAATTCTGGTCTAAATTGTGGGACTCAAAGGAGACTAGACTCTAATAGGATTGGTAGAAGAAA TTACAGTTCACCACCTCCCTGTCACCTTTCCAGGCAGGTCCCTTATGATGAAATCTCTGCTGTTCATCAG CATAGTTATCATCCGTCAGGAAGCAAACCTAAGAGTCAGCAGACGTCTTTCCAGTCCTCTCCTTGTAATA AATCGCCCAAGAGCCATGGCCTTCAGAATCAACCTTGGCAGAAATTGAGGAATGAGAAGCACCATATCAG AGTCAAGAAAGCACAGAGTCTTGCTGAGCAGACCTCAGATACAGCTGGATTAGAGAGCTCGACCAGATCA GAGAGTGGGACAGACCTCAGAGAGCATAGTCCTTCTGAGAGTGAGAAGGAAGTTGTGGGTGCAGATCCCA GGGGAGCAAAACCCAAAAAAGCAACACAGTTTGTATACAGCTATGGTAGAGGACCAAAAGTCAAGGGGAA ACTCAAATGTGAATGGAGTAACCGAACAACTCCAAAACCGGAGGATGCTGGACCCGAAAGTACCAAACCT GTGGGGGTTTTCCACCCTGACTCTTCAGAGGCATCCTCTAGAAAAGGAGTATTGGATGGGTATGGAGCCA GACGAAATGAGCAGAGAAGATACCCACAGAAAAGGCCTCCCTGGGAAGTGGAGGGGGCCAGGCCACGACC AGGCAGAAATCCACCAAAACAGGAGGGCCACCGACATACAAACGCAGGACACAGAAACAACATGGGCCCC ATTCCAAAGGATGACCTCAATGAAAGACCAGCAAAATCTACCTGTGACAGTGAGAACTTGGCAGTCATCA ACAAGTCTTCCAGGAGGGTTGACCAAGAGAAATGCACTGTACGGAGGCAGGATCCTCAAGTAGTATCTCC TTTCTCCCGAGGCAAACAGAACCATGTGCTAAAGAATGTGGAAACGCACACAGGTTCTCTAATTGAACAA CTAACAACAGAAAAATACGAGTGCATGGTGTGCTGTGAATTGGTTCGTGTCACGGCCCCAGTGTGGAGTT GTCAGAGCTGTTACCATGTGTTTCATTTGAACTGCATAAAGAAATGGGCAAGGTCTCCAGCATCTCAAGC AGATGGCCAGAGTGGTTGGAGGTGCCCTGCCTGTCAGAATGTTTCTGCACATGTTCCTAATACCTACACT TGTTTCTGTGGCAAGGTAAAGAATCCTGAGTGGAGCAGAAATGAAATTCCACATAGCTGTGGTGAGGTTT GTAGAAAGAAACAGCCTGGCCAGGACTGCCCACATTCCTGTAACCTTCTCTGCCATCCAGGACCCTGCCC ACCCTGCCCTGCCTTTATGACAAAAACATGTGAATGTGGACGAACCAGGCACACAGTTCGCTGTGGTCAG GCTGTCTCAGTCCACTGTTCTAACCCATGTGAGAATATTTTGAACTGTGGTCAGCACCAGTGTGCTGAGC TGTGCCATGGGGGTCAGTGCCAGCCTTGCCAGATCATTTTGAACCAGGTATGCTATTGCGGCAGCACCTC CCGAGATGTGTTATGTGGAACCGATGTAGGAAAGTCTGATGGATTTGGGGATTTCAGCTGTTTAAAGATA TGTGGCAAGGACTTGAAATGCGGTAACCATACATGTTCGCAAGTGTGCCACCCTCAGCCCTGCCAGCAAT GCCCACGGCTCCCCCAGCTGGTGCGCTGTTGCCCCTGTGGCCAAACTCCTCTCAGCCAATTGCTAGAACT TGGAAGTAGTAGTCGGAAAACATGCATGGACCCTGTGCCTTCATGTGGAAAAGTGTGCGGCAAGCCTCTG CCTTGTGGTTCCTTAGATTTCATTCATACCTGTGAAAAGCTCTGCCATGAAGGAGACTGTGGACCATGCT CTCGCACATCAGTTATTTCCTGCAGATGCTCTTTCAGAACAAAGGAGCTTCCATGTACCAGTCTCAAAAG TGAAGATGCTACATTTATGTGTGACAAGCGGTGTAACAAGAAACGGTTGTGTGGACGGCATAAATGTAAT GAGATATGCTGTGTGGATAAGGAGCACAAGTGTCCTTTGATTTGTGGGAGGAAACTCCGTTGTGGCCTTC ATAGGTGTGAAGAACCTTGTCATCGTGGAAACTGCCAGACATGCTGGCAAGCCAGTTTTGATGAATTAAC CTGCCATTGTGGTGCATCAGTGATTTACCCTCCAGTTCCCTGTGGTACTAGGCCCCCTGAATGTACCCAA ACCTGCGCTAGAGTCCATGAGTGTGACCATCCAGTATATCATTCTTGTCATAGTGAGGAGAAGTGTCCCC CTTGCACTTTCCTAACTCAGAAGTGGTGCATGGGCAAGCATGAGCAGTCCCACTACTGGGCGTCTACCCA GAAGAAAAGAAGTCATTATATGAAAAAGATACCTGCACACGCATGTTTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC221810 representing NM_147134
Red=Cloning site Green=Tags(s) MAEAPPVSGTFKFNTDAAEFIPQEKKNSGLNCGTQRRLDSNRIGRRNYSSPPPCHLSRQVPYDEISAVHQ HSYHPSGSKPKSQQTSFQSSPCNKSPKSHGLQNQPWQKLRNEKHHIRVKKAQSLAEQTSDTAGLESSTRS ESGTDLREHSPSESEKEVVGADPRGAKPKKATQFVYSYGRGPKVKGKLKCEWSNRTTPKPEDAGPESTKP VGVFHPDSSEASSRKGVLDGYGARRNEQRRYPQKRPPWEVEGARPRPGRNPPKQEGHRHTNAGHRNNMGP IPKDDLNERPAKSTCDSENLAVINKSSRRVDQEKCTVRRQDPQVVSPFSRGKQNHVLKNVETHTGSLIEQ LTTEKYECMVCCELVRVTAPVWSCQSCYHVFHLNCIKKWARSPASQADGQSGWRCPACQNVSAHVPNTYT CFCGKVKNPEWSRNEIPHSCGEVCRKKQPGQDCPHSCNLLCHPGPCPPCPAFMTKTCECGRTRHTVRCGQ AVSVHCSNPCENILNCGQHQCAELCHGGQCQPCQIILNQVCYCGSTSRDVLCGTDVGKSDGFGDFSCLKI CGKDLKCGNHTCSQVCHPQPCQQCPRLPQLVRCCPCGQTPLSQLLELGSSSRKTCMDPVPSCGKVCGKPL PCGSLDFIHTCEKLCHEGDCGPCSRTSVISCRCSFRTKELPCTSLKSEDATFMCDKRCNKKRLCGRHKCN EICCVDKEHKCPLICGRKLRCGLHRCEEPCHRGNCQTCWQASFDELTCHCGASVIYPPVPCGTRPPECTQ TCARVHECDHPVYHSCHSEEKCPPCTFLTQKWCMGKHEQSHYWASTQKKRSHYMKKIPAHACL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_147134 |
| ORF Size | 2499 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_147134.4 |
| RefSeq Size | 3638 bp |
| RefSeq ORF | 2502 bp |
| Locus ID | 4799 |
| UniProt ID | Q12986 |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 92.7 kDa |
| Gene Summary | MHC class II gene expression is controlled primarily at the transcriptional level by transcription factors that bind to the X and Y boxes, two highly conserved elements in the proximal promoter of MHC class II genes. The protein encoded by this gene is a transcriptional repressor capable of binding to the conserved X box motif of HLA-DRA and other MHC class II genes in vitro. The protein may play a role in regulating the duration of an inflammatory response by limiting the period in which class II MHC molecules are induced by IFN-gamma. Three alternative splice variants, each of which encodes a different isoform, have been identified. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221810L3 | Lenti-ORF clone of NFX1 (Myc-DDK-tagged)-Human nuclear transcription factor, X-box binding 1 (NFX1), transcript variant 3 |
CNY 10,640.00 |
|
| RC221810L4 | Lenti-ORF clone of NFX1 (mGFP-tagged)-Human nuclear transcription factor, X-box binding 1 (NFX1), transcript variant 3 |
CNY 10,640.00 |
|
| RG221810 | NFX1 (tGFP-tagged) - Human nuclear transcription factor, X-box binding 1 (NFX1), transcript variant 3 |
CNY 9,600.00 |
|
| SC314793 | NFX1 (untagged)-Human nuclear transcription factor, X-box binding 1 (NFX1), transcript variant 3 |
CNY 16,060.00 |
