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P2RX7 (NM_002562) Human Tagged ORF Clone
CAT＃: RC222143
P2RX7 (Myc-DDK-tagged)-Human purinergic receptor P2X, ligand-gated ion channel, 7 (P2RX7), transcript variant 1
ORF Plasmid: tGFP
"NM_002562" in other vectors (6)
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CNY 4,432.00
CNY 5,890.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | P2X7 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC222143 representing NM_002562
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGCCTGCTGCAGCTGCAGTGATGTTTTCCAGTATGAGACGAACAAAGTCACTCGGATCCAGAGCA TGAATTATGGCACCATTAAGTGGTTCTTCCACGTGATCATCTTTTCCTACGTTTGCTTTGCTCTGGTGAG TGACAAGCTGTACCAGCGGAAAGAGCCTGTCATCAGTTCTGTGCACACCAAGGTGAAGGGGATAGCAGAG GTGAAAGAGGAGATCGTGGAGAATGGAGTGAAGAAGTTGGTGCACAGTGTCTTTGACACCGCAGACTACA CCTTCCCTTTGCAGGGGAACTCTTTCTTCGTGATGACAAACTTTCTCAAAACAGAAGGCCAAGAGCAGCG GTTGTGTCCCGAGTATCCCACCCGCAGGACGCTCTGTTCCTCTGACCGAGGTTGTAAAAAGGGATGGATG GACCCGCAGAGCAAAGGAATTCAGACCGGAAGGTGTGTAGTGTATGAAGGGAACCAGAAGACCTGTGAAG TCTCTGCCTGGTGCCCCATCGAGGCAGTGGAAGAGGCCCCCCGGCCTGCTCTCTTGAACAGTGCCGAAAA CTTCACTGTGCTCATCAAGAACAATATCGACTTCCCCGGCCACAACTACACCACGAGAAACATCCTGCCA GGTTTAAACATCACTTGTACCTTCCACAAGACTCAGAATCCACAGTGTCCCATTTTCCGACTAGGAGACA TCTTCCGAGAAACAGGCGATAATTTTTCAGATGTGGCAATTCAGGGCGGAATAATGGGCATTGAGATCTA CTGGGACTGCAACCTAGACCGTTGGTTCCATCACTGCCGTCCCAAATACAGTTTCCGTCGCCTTGACGAC AAGACCACCAACGTGTCCTTGTACCCTGGCTACAACTTCAGATACGCCAAGTACTACAAGGAAAACAATG TTGAGAAACGGACTCTGATAAAAGTCTTCGGGATCCGTTTTGACATCCTGGTTTTTGGCACCGGAGGAAA ATTTGACATTATCCAGCTGGTTGTGTACATCGGCTCAACCCTCTCCTACTTCGGTCTGGCCGCTGTGTTC ATCGACTTCCTCATCGACACTTACTCCAGTAACTGCTGTCGCTCCCATATTTATCCCTGGTGCAAGTGCT GTCAGCCCTGTGTGGTCAACGAATACTACTACAGGAAGAAGTGCGAGTCCATTGTGGAGCCAAAGCCGAC ATTAAAGTATGTGTCCTTTGTGGATGAATCCCACATTAGGATGGTGAACCAGCAGCTACTAGGGAGAAGT CTGCAAGATGTCAAGGGCCAAGAAGTCCCAAGACCTGCGATGGACTTCACAGATTTGTCCAGGCTGCCCC TGGCCCTCCATGACACACCCCCGATTCCTGGACAACCAGAGGAGATACAGCTGCTTAGAAAGGAGGCGAC TCCTAGATCCAGGGATAGCCCCGTCTGGTGCCAGTGTGGAAGCTGCCTCCCATCTCAACTCCCTGAGAGC CACAGGTGCCTGGAGGCGCTGTGCTGCCGGAAAAAGCCGGGGGCCTGCATCACCACCTCAGAGCTGTTCA GGAAGCTGGTCCTGTCCAGACACGTCCTGCAGTTCCTCCTGCTCTACCAGGAGCCCTTGCTGGCGCTGGA TGTGGATTCCACCAACAGCCGGCTGCGGCACTGTGCCTACAGGTGCTACGCCACCTGGCGCTTCGGCTCC CAGGACATGGCTGACTTTGCCATCCTGCCCAGCTGCTGCCGCTGGAGGATCCGGAAAGAGTTTCCGAAGA GTGAAGGGCAGTACAGTGGCTTCAAGAGTCCTTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC222143 representing NM_002562
Red=Cloning site Green=Tags(s) MPACCSCSDVFQYETNKVTRIQSMNYGTIKWFFHVIIFSYVCFALVSDKLYQRKEPVISSVHTKVKGIAE VKEEIVENGVKKLVHSVFDTADYTFPLQGNSFFVMTNFLKTEGQEQRLCPEYPTRRTLCSSDRGCKKGWM DPQSKGIQTGRCVVYEGNQKTCEVSAWCPIEAVEEAPRPALLNSAENFTVLIKNNIDFPGHNYTTRNILP GLNITCTFHKTQNPQCPIFRLGDIFRETGDNFSDVAIQGGIMGIEIYWDCNLDRWFHHCRPKYSFRRLDD KTTNVSLYPGYNFRYAKYYKENNVEKRTLIKVFGIRFDILVFGTGGKFDIIQLVVYIGSTLSYFGLAAVF IDFLIDTYSSNCCRSHIYPWCKCCQPCVVNEYYYRKKCESIVEPKPTLKYVSFVDESHIRMVNQQLLGRS LQDVKGQEVPRPAMDFTDLSRLPLALHDTPPIPGQPEEIQLLRKEATPRSRDSPVWCQCGSCLPSQLPES HRCLEALCCRKKPGACITTSELFRKLVLSRHVLQFLLLYQEPLLALDVDSTNSRLRHCAYRCYATWRFGS QDMADFAILPSCCRWRIRKEFPKSEGQYSGFKSPY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002562 |
| ORF Size | 1785 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002562.6 |
| RefSeq Size | 3155 bp |
| RefSeq ORF | 1788 bp |
| Locus ID | 5027 |
| UniProt ID | Q99572 |
| Domains | P2X_receptor |
| Protein Families | Druggable Genome, Ion Channels: ATP Receptors, Transmembrane |
| Protein Pathways | Calcium signaling pathway, Neuroactive ligand-receptor interaction |
| MW | 68.4 kDa |
| Gene Summary | The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression. Multiple alternatively spliced variants have been identified, most of which fit nonsense-mediated decay (NMD) criteria. [provided by RefSeq, Jul 2010] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
EMP2 induces cytostasis and apoptosis via the TGFβ/SMAD/SP1 axis and recruitment of P2RX7 in urinary bladder urothelial carcinoma
,null,
Cellular Oncology
,PubMed ID 34339014
[P2RX7]
|
|
Streptococcus pneumoniae disrupts pulmonary immune defence via elastase release following pneumolysin-dependent neutrophil lysis
,Domon, H;Oda, M;Maekawa, T;Nagai, K;Takeda, W;Terao, Y;,
Sci Rep
,PubMed ID 27892542
[P2RX7]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222143L1 | Lenti ORF clone of Human purinergic receptor P2X, ligand-gated ion channel, 7 (P2RX7), transcript variant 1, Myc-DDK-tagged |
CNY 6,832.00 |
|
| RC222143L2 | Lenti ORF clone of Human purinergic receptor P2X, ligand-gated ion channel, 7 (P2RX7), transcript variant 1, mGFP tagged |
CNY 7,790.00 |
|
| RC222143L3 | Lenti ORF clone of Human purinergic receptor P2X, ligand-gated ion channel, 7 (P2RX7), transcript variant 1, Myc-DDK-tagged |
CNY 6,832.00 |
|
| RC222143L4 | Lenti ORF clone of Human purinergic receptor P2X, ligand-gated ion channel, 7 (P2RX7), transcript variant 1, mGFP tagged |
CNY 6,832.00 |
|
| RG222143 | P2RX7 (tGFP-tagged) - Human purinergic receptor P2X, ligand-gated ion channel, 7 (P2RX7), transcript variant 1 |
CNY 6,032.00 |
|
| SC310230 | P2RX7 (untagged)-Human purinergic receptor P2X, ligand-gated ion channel, 7 (P2RX7), transcript variant 1 |
CNY 4,440.00 |
