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AGO3 (NM_024852) Human Tagged ORF Clone
CAT＃: RC222233
AGO3 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 2C, 3 (EIF2C3), transcript variant 1
ORF Plasmid: tGFP
"NM_024852" in other vectors (6)
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CNY 9,448.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | EIF2C3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC222233 representing NM_024852
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAATCGGCTCCGCAGGACCCGCTGGGGCCCAGCCCCTACTCATGGTGCCCAGAAGACCTGGCTATG GCACCATGGGCAAACCCATTAAACTGCTGGCTAACTGTTTTCAAGTTGAAATCCCAAAGATTGATGTCTA CCTCTATGAGGTAGATATTAAACCAGACAAGTGTCCTAGGAGAGTGAACAGGGAGGTGGTTGACTCAATG GTTCAGCATTTTAAAGTAACTATATTTGGAGACCGTAGACCAGTTTATGATGGAAAAAGAAGTCTTTACA CCGCCAATCCACTTCCTGTGGCAACTACAGGGGTAGATTTAGACGTTACTTTACCTGGGGAAGGTGGAAA AGATCGACCTTTCAAGGTGTCAATCAAATTTGTCTCTCGGGTGAGTTGGCACCTACTGCATGAAGTACTG ACAGGACGGACCTTGCCTGAGCCACTGGAATTAGACAAGCCAATCAGCACTAACCCTGTCCATGCCGTTG ATGTGGTGCTACGACATCTGCCCTCCATGAAATACACACCTGTGGGGCGTTCATTTTTCTCCGCTCCAGA AGGATATGACCACCCTCTGGGAGGGGGCAGGGAAGTGTGGTTTGGATTCCATCAGTCTGTTCGGCCTGCC ATGTGGAAAATGATGCTTAATATCGATGTTTCTGCCACTGCCTTCTACAAAGCACAACCTGTAATTCAGT TCATGTGTGAAGTTCTTGATATTCATAATATTGATGAGCAACCAAGACCTCTGACTGATTCTCATCGGGT AAAATTCACCAAAGAGATAAAAGGTTTGAAGGTTGAAGTGACTCATTGTGGAACAATGAGACGGAAATAC CGTGTTTGTAATGTAACAAGGAGGCCTGCCAGTCATCAAACCTTTCCTTTACAGTTAGAAAACGGCCAAA CTGTGGAGAGAACAGTAGCGCAGTATTTCAGAGAAAAGTATACTCTTCAGCTGAAGTACCCGCACCTTCC CTGTCTGCAAGTCGGGCAGGAACAGAAACACACCTACCTGCCACTAGAAGTCTGTAATATTGTGGCAGGG CAACGATGTATCAAGAAGCTAACAGACAATCAGACTTCCACTATGATCAAGGCAACAGCAAGATCTGCAC CAGATAGACAAGAGGAAATTAGCAGATTGGTAAGAAGTGCAAATTATGAAACAGATCCATTTGTTCAGGA GTTTCAATTTAAAGTTCGGGATGAAATGGCTCATGTAACTGGACGCGTACTTCCAGCACCTATGCTCCAG TATGGAGGACGGAATCGGACAGTAGCAACACCGAGCCATGGAGTATGGGACATGCGAGGGAAACAATTCC ACACAGGAGTTGAAATCAAAATGTGGGCTATCGCTTGTTTTGCCACACAGAGGCAGTGCAGAGAAGAAAT ATTGAAGGGTTTCACAGACCAGCTGCGTAAGATTTCTAAGGATGCAGGGATGCCCATCCAGGGCCAGCCA TGCTTCTGCAAATATGCACAGGGGGCAGACAGCGTAGAGCCCATGTTCCGGCATCTCAAGAACACATATT CTGGCCTACAGCTTATTATCGTCATCCTGCCGGGGAAGACACCAGTGTATGCGGAAGTGAAACGTGTAGG AGACACACTTTTGGGTATGGCTACACAATGTGTTCAAGTCAAGAATGTAATAAAAACATCTCCTCAAACT CTGTCAAACTTGTGCCTAAAGATAAATGTTAAACTCGGAGGGATCAATAATATTCTTGTACCTCATCAAA GACCTTCTGTGTTCCAGCAACCAGTGATCTTTTTGGGAGCCGATGTCACTCATCCACCTGCTGGTGATGG AAAGAAGCCTTCTATTGCTGCTGTTGTAGGTAGTATGGATGCACACCCAAGCAGATACTGTGCCACAGTA AGAGTTCAGAGACCCCGACAGGAGATCATCCAGGACTTGGCCTCCATGGTCCGGGAACTTCTTATTCAAT TTTATAAGTCAACTCGGTTCAAGCCTACTCGTATCATCTTTTATCGGGATGGTGTTTCAGAGGGGCAGTT TAGGCAGGTATTATATTATGAACTACTAGCAATTCGAGAAGCCTGCATCAGTTTGGAGAAAGACTATCAA CCTGGAATAACCTACATTGTAGTTCAGAAGAGACATCACACTCGATTATTTTGTGCTGATAGGACAGAAA GGGTTGGAAGAAGTGGCAATATCCCAGCTGGAACAACAGTTGATACAGACATTACACACCCATATGAGTT CGATTTTTACCTCTGTAGCCATGCTGGAATACAGGGTACCAGTCGTCCTTCACACTATCATGTTTTATGG GATGATAACTGCTTTACTGCAGATGAACTTCAGCTGCTAACTTACCAGCTCTGCCACACTTACGTACGCT GTACACGATCTGTTTCTATACCTGCACCAGCGTATTATGCTCACCTGGTAGCATTTAGAGCCAGATATCA TCTTGTGGACAAAGAACATGACAGTGCTGAAGGAAGTCACGTTTCAGGACAAAGCAATGGGCGAGATCCA CAAGCTCTTGCCAAGGCTGTACAGATTCACCAAGATACCTTACGCACAATGTACTTCGCT AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC222233 representing NM_024852
Red=Cloning site Green=Tags(s) MEIGSAGPAGAQPLLMVPRRPGYGTMGKPIKLLANCFQVEIPKIDVYLYEVDIKPDKCPRRVNREVVDSM VQHFKVTIFGDRRPVYDGKRSLYTANPLPVATTGVDLDVTLPGEGGKDRPFKVSIKFVSRVSWHLLHEVL TGRTLPEPLELDKPISTNPVHAVDVVLRHLPSMKYTPVGRSFFSAPEGYDHPLGGGREVWFGFHQSVRPA MWKMMLNIDVSATAFYKAQPVIQFMCEVLDIHNIDEQPRPLTDSHRVKFTKEIKGLKVEVTHCGTMRRKY RVCNVTRRPASHQTFPLQLENGQTVERTVAQYFREKYTLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAG QRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVRSANYETDPFVQEFQFKVRDEMAHVTGRVLPAPMLQ YGGRNRTVATPSHGVWDMRGKQFHTGVEIKMWAIACFATQRQCREEILKGFTDQLRKISKDAGMPIQGQP CFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVIKTSPQT LSNLCLKINVKLGGINNILVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATV RVQRPRQEIIQDLASMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFRQVLYYELLAIREACISLEKDYQ PGITYIVVQKRHHTRLFCADRTERVGRSGNIPAGTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYHVLW DDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHVSGQSNGRDP QALAKAVQIHQDTLRTMYFA SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_024852 |
| ORF Size | 2580 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_024852.4 |
| RefSeq Size | 3578 bp |
| RefSeq ORF | 2583 bp |
| Locus ID | 192669 |
| UniProt ID | Q9H9G7 |
| Domains | PAZ, Piwi |
| Protein Families | Druggable Genome |
| MW | 97.2 kDa |
| Gene Summary | This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, contains a PAZ domain and a PIWI domain, and may play a role in short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a tandem cluster of closely related family members including argonaute 4 and eukaryotic translation initiation factor 2C, 1. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222233L1 | Lenti ORF clone of Human eukaryotic translation initiation factor 2C, 3 (EIF2C3), transcript variant 1, Myc-DDK-tagged |
CNY 11,848.00 |
|
| RC222233L2 | Lenti ORF clone of Human eukaryotic translation initiation factor 2C, 3 (EIF2C3), transcript variant 1, mGFP tagged |
CNY 9,030.00 |
|
| RC222233L3 | Lenti ORF clone of Human eukaryotic translation initiation factor 2C, 3 (EIF2C3), transcript variant 1, Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC222233L4 | Lenti ORF clone of Human eukaryotic translation initiation factor 2C, 3 (EIF2C3), transcript variant 1, mGFP tagged |
CNY 9,030.00 |
|
| RG222233 | AGO3 (tGFP-tagged) - Human eukaryotic translation initiation factor 2C, 3 (EIF2C3), transcript variant 1 |
CNY 11,048.00 |
|
| SC310079 | AGO3 (untagged)-Human eukaryotic translation initiation factor 2C, 3 (EIF2C3), transcript variant 1 |
CNY 9,456.00 |
