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Choline Acetyltransferase (CHAT) (NM_020549) Human Tagged ORF Clone
CAT＃: RC222531
CHAT (Myc-DDK-tagged)-Human choline O-acetyltransferase (CHAT), transcript variant M
ORF Plasmid: tGFP
"NM_020549" in other vectors (4)
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CNY 5,480.00
CNY 7,890.00
Product images
CNY 300.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CHOACTASE; CMS1A; CMS1A2; CMS6 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC222531 representing NM_020549
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCTGAGGACAGCGAAGAAGAGGGGGCTTGGGGGAGGGGGGAAATGGAAGAGAGAGGAGGGAGGAG GTACAAGAGGAAGGAGAGAAGTGCGGCCAGCTTGCTTTCTCCAGTCGGGTGGCCGCGGGGACCCGGGCGA CGTCGGAGGCCCTGCCGGGAACCCAGGCTGCAGCCCCCACCCCCGCGCTGCGACACGCCCCCCACCCCTT CCGGCTCACACCCCCGCCCACACTCCTGAGTGGTGCGGTGCAGCGTCGGCCGAGGCAGCAGAGCCGAGGA GAGCAGGTCCACACCTCTGCATCCCTGCACCAGGACTCACCAAGACGCCCATCCTGGAAAAGGTCCCCCG TAAGATGGCAGCAAAAACTCCCAGCAGTGAGGAGTCTGGGCTGCCCAAACTGCCCGTGCCCCCGCTGCAG CAGACCCTGGCCACGTACCTGCAGTGCATGCGACACTTGGTGTCTGAGGAGCAGTTCAGGAAGAGCCAGG CCATTGTGCAGCAGTTTGGGGCCCCTGGTGGCCTCGGCGAGACCCTGCAGCAGAAACTCCTGGAGCGGCA GGAGAAGACAGCCAACTGGGTGTCTGAGTACTGGCTGAATGACATGTATCTCAACAACCGCCTGGCCCTG CCTGTCAACTCCAGCCCTGCCGTGATCTTTGCTCGGCAGCACTTCCCTGGCACCGATGACCAGCTGAGGT TTGCAGCCAGCCTCATCTCTGGTGTACTCAGCTACAAGGCCCTGCTGGACAGCCACTCCATTCCCACTGA CTGTGCCAAAGGCCAGCTGTCAGGGCAGCCCCTTTGCATGAAGCAATACTATGGGCTCTTCTCCTCCTAC CGGCTCCCCGGCCATACCCAGGACACGCTGGTGGCTCAGAACAGCAGCATCATGCCGGAGCCTGAGCACG TCATCGTAGCCTGCTGCAATCAGTTCTTTGTCTTGGATGTTGTCATTAATTTCCGCCGTCTCAGTGAGGG GGATCTGTTCACTCAGTTGAGAAAGATAGTCAAAATGGCTTCCAACGAGGACGAGCGTTTGCCTCCAATT GGCCTGCTGACGTCTGACGGGAGGAGCGAGTGGGCCGAGGCCAGGACGGTCCTCGTGAAAGACTCCACCA ACCGGGACTCGCTGGACATGATTGAGCGCTGCATCTGCCTTGTATGCCTGGACGCGCCAGGAGGCGTGGA GCTCAGCGACACCCACAGGGCACTCCAGCTCCTTCACGGCGGAGGCTACAGCAAGAACGGGGCCAATCGC TGGTACGACAAGTCCCTGCAGTTTGTGGTGGGCCGAGACGGCACCTGCGGTGTGGTGTGCGAACACTCCC CATTCGATGGCATCGTCCTGGTGCAGTGCACTGAGCATCTGCTCAAGCACATGACGCAGAGCAGCAGGAA GCTGATCCGAGCAGACTCCGTCAGCGAGCTCCCCGCCCCCCGGAGGCTGCGGTGGAAATGCTCCCCGGAA ATTCAAGGCCACTTAGCCTCCTCGGCAGAAAAACTTCAACGAATAGTAAAGAACCTTGACTTCATTGTCT ATAAGTTTGACAACTATGGGAAAACATTCATTAAGAAGCAGAAATGCAGCCCTGATGCCTTCATCCAGGT GGCCCTCCAGCTGGCCTTCTACAGGCTCCATCGAAGACTGGTGCCCACCTACGAGAGCGCGTCCATCCGC CGATTCCAGGAGGGACGCGTGGACAACATCAGATCGGCCACTCCAGAGGCACTGGCTTTTGTGAGAGCCG TGACTGACCACAAGGCTGCTGTGCCAGCTTCTGAGAAGCTTCTGCTCCTGAAGGATGCCATCCGTGCCCA GACTGCATACACAGTCATGGCCATAACAGGGATGGCCATTGACAACCACCTGCTGGCACTGCGGGAGCTG GCCCGGGCCATGTGCAAGGAGCTGCCCGAGATGTTCATGGATGAAACCTACCTGATGAGCAACCGGTTTG TCCTCTCCACTAGCCAGGTGCCCACAACCACGGAGATGTTCTGCTGCTATGGTCCTGTGGTCCCAAATGG GTATGGTGCCTGCTACAACCCCCAGCCAGAGACCATCCTTTTCTGCATCTCTAGCTTTCACAGCTGCAAA GAGACTTCTTCTAGCAAGTTTGCAAAAGCTGTGGAAGAAAGCCTCATTGACATGAGAGACCTCTGCAGTC TGCTGCCGCCTACTGAGAGCAAGCCATTGGCAACAAAGGAAAAAGCCACGAGGCCCAGCCAGGGACACCA ACCT AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC222531 representing NM_020549
Red=Cloning site Green=Tags(s) MGLRTAKKRGLGGGGKWKREEGGGTRGRREVRPACFLQSGGRGDPGDVGGPAGNPGCSPHPRAATRPPPL PAHTPAHTPEWCGAASAEAAEPRRAGPHLCIPAPGLTKTPILEKVPRKMAAKTPSSEESGLPKLPVPPLQ QTLATYLQCMRHLVSEEQFRKSQAIVQQFGAPGGLGETLQQKLLERQEKTANWVSEYWLNDMYLNNRLAL PVNSSPAVIFARQHFPGTDDQLRFAASLISGVLSYKALLDSHSIPTDCAKGQLSGQPLCMKQYYGLFSSY RLPGHTQDTLVAQNSSIMPEPEHVIVACCNQFFVLDVVINFRRLSEGDLFTQLRKIVKMASNEDERLPPI GLLTSDGRSEWAEARTVLVKDSTNRDSLDMIERCICLVCLDAPGGVELSDTHRALQLLHGGGYSKNGANR WYDKSLQFVVGRDGTCGVVCEHSPFDGIVLVQCTEHLLKHMTQSSRKLIRADSVSELPAPRRLRWKCSPE IQGHLASSAEKLQRIVKNLDFIVYKFDNYGKTFIKKQKCSPDAFIQVALQLAFYRLHRRLVPTYESASIR RFQEGRVDNIRSATPEALAFVRAVTDHKAAVPASEKLLLLKDAIRAQTAYTVMAITGMAIDNHLLALREL ARAMCKELPEMFMDETYLMSNRFVLSTSQVPTTTEMFCCYGPVVPNGYGACYNPQPETILFCISSFHSCK ETSSSKFAKAVEESLIDMRDLCSLLPPTESKPLATKEKATRPSQGHQP SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020549 |
| ORF Size | 2244 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_020549.4, NP_065574.3 |
| RefSeq Size | 2485 bp |
| RefSeq ORF | 2247 bp |
| Locus ID | 1103 |
| UniProt ID | P28329 |
| Protein Families | Druggable Genome |
| Protein Pathways | Glycerophospholipid metabolism |
| MW | 82.4 kDa |
| Gene Summary | This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222531L3 | Lenti ORF clone of Human choline O-acetyltransferase (CHAT), transcript variant M, Myc-DDK-tagged |
CNY 9,790.00 |
|
| RC222531L4 | Lenti ORF clone of Human choline O-acetyltransferase (CHAT), transcript variant M, mGFP tagged |
CNY 9,790.00 |
|
| RG222531 | CHAT (tGFP-tagged) - Human choline O-acetyltransferase (CHAT), transcript variant M |
CNY 8,650.00 |
|
| SC304784 | CHAT (untagged)-Human choline O-acetyltransferase (CHAT), transcript variant M |
CNY 14,350.00 |
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