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DOCK8 (NM_203447) Human Tagged ORF Clone
CAT＃: RC222789
DOCK8 (Myc-DDK-tagged)-Human dedicator of cytokinesis 8 (DOCK8), transcript variant 1
ORF Plasmid: tGFP
"NM_203447" in other vectors (6)
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CNY 20,112.00
| Cited in 1 publication. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HEL-205; MRD2; ZIR8 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC222789 representing NM_203447
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACACACCTGAACAGCCTGGATGTGCAGCTTGCCCAGGAGCTCGGGGACTTCACTGATGACGACTTGG ACGTGGTGTTCACGCCAAAGGAATGTAGGACTTTGCAGCCCTCTTTGCCGGAGGAAGGGGTTGAACTGGA CCCTCATGTCAGGGACTGTGTTCAGACCTACATCCGTGAGTGGCTAATCGTGAACCGGAAAAACCAAGGA AGTCCAGAAATCTGTGGCTTTAAAAAGACTGGATCTCGAAAAGATTTTCACAAGACGCTTCCGAAACAGA CGTTTGAGTCGGAAACCTTGGAGTGCAGTGAACCCGCTGCTCAGGCAGGCCCCCGCCACTTAAACGTGCT GTGCGACGTGTCTGGGAAAGGCCCCGTCACTGCCTGTGACTTTGACCTCCGCAGCCTGCAGCCTGACAAG CGGCTAGAAAACCTCCTGCAGCAAGTGAGTGCCGAGGACTTTGAGAAGCAGAACGAGGAGGCCCGGAGGA CCAATAGGCAGGCCGAGCTCTTTGCCCTTTACCCATCAGTGGACGAGGAGGATGCTGTGGAAATACGTCC AGTACCAGAATGTCCCAAGGAACACCTGGGCAACAGAATATTGGTCAAGTTGCTGACCTTGAAGTTCGAG ATTGAAATTGAGCCCCTGTTTGCCAGCATTGCCCTCTACGATGTTAAAGAAAGGAAAAAGATCTCAGAAA ATTTTCACTGTGACCTGAACTCTGACCAGTTCAAAGGATTTCTGCGAGCTCACACGCCTTCAGTGGCCGC ATCAAGTCAGGCGAGATCTGCAGTCTTCTCAGTCACCTACCCGTCCTCAGACATCTACCTGGTAGTCAAG ATTGAAAAAGTCCTGCAGCAGGGAGAGATTGGAGACTGTGCAGAGCCCTACACGGTTATCAAAGAAAGTG ATGGTGGAAAGAGTAAAGAAAAGATTGAAAAACTAAAACTCCAAGCTGAATCCTTCTGCCAGCGTTTGGG GAAATACCGGATGCCCTTTGCCTGGGCACCCATAAGCTTATCAAGCTTCTTCAGTGTCTCCACCCTTGAG AGGGAGGTAACTGATGTGGACTCTGTGGTTGGGAGAAGCTCAGTGGGTGAACGGAGGACATTGGCCCAAT CTAGAAGGCTTTCTGAAAGAGCCCTCTCCTTGGAGGAAAATGGGGTTGGATCCAACTTCAAAACCTCCAC TCTGAGCGTTAGCATCTTTTTCAAGCAGGAAGGAGATCGCCTTAGCGATGAAGACTTATTCAAGTTTTTA GCTGACTACAAAAGATCATCATCCTTACAGAGACGAGTCAAGTCAATTCCAGGCTTGCTAAGACTGGAGA TTTCTACAGCTCCAGAGATCATCAATTGCTGTCTGACTCCTGAAATGCTGCCCGTGAAACCCTTTCCTGA AAACCGGACACGCCCGCACAAAGAGATTTTGGAATTTCCAACACGAGAAGTATATGTCCCTCACACTGTG TACAGAAACCTTCTCTATGTCTACCCACAGAGGCTGAACTTTGTAAACAAACTAGCATCAGCCCGGAACA TTACAATAAAGATCCAGTTTATGTGTGGAGAAGATGCTAGCAATGCGATGCCGGTCATCTTTGGAAAGTC CAGCGGGCCTGAATTTCTGCAGGAAGTGTACACAGCTGTTACATACCATAATAAGTCTCCTGACTTTTAT GAAGAAGTGAAAATTAAGCTCCCCGCTAAGCTCACAGTAAATCACCACCTCCTGTTCACCTTCTACCATA TCAGCTGTCAGCAGAAGCAAGGAGCCTCCGTGGAAACTCTCCTGGGATATTCATGGCTGCCAATTCTCTT AAATGAACGTCTTCAAACTGGATCCTACTGTCTCCCAGTTGCCTTGGAAAAATTGCCACCCAACTACTCC ATGCATTCTGCTGAGAAAGTCCCATTACAGAATCCTCCCATTAAGTGGGCTGAAGGACATAAGGGAGTAT TTAATATTGAAGTGCAAGCTGTTTCTTCTGTACACACCCAGGACAACCACCTGGAGAAGTTCTTCACCCT CTGCCACTCCCTGGAGAGCCAGGTGACCTTCCCCATCCGCGTGCTGGATCAGAAAATCAGCGAGATGGCG CTGGAGCATGAGCTGAAGCTCAGCATCATCTGCCTGAACTCCTCCCGCCTGGAGCCGCTCGTGCTCTTCC TGCACCTGGTGCTGGACAAGCTCTTCCAGCTGTCCGTGCAGCCCATGGTCATCGCTGGCCAGACAGCCAA CTTCTCCCAGTTTGCCTTCGAGTCCGTGGTGGCCATCGCCAACAGTCTGCACAACAGCAAGGACCTGAGC AAGGACCAGCATGGGAGGAACTGCCTGCTGGCTTCCTACGTGCACTACGTCTTCCGCCTGCCAGAGGTGC AAAGGGATGTGCCCAAGTCAGACGCTCCCACTGCCCTCCTAGACCCTCGGAGCTACCACACGTATGGCCG CACATCAGCTGCTGCTGTGAGTTCAAAGCTGCTGCAGGCCCGGGTGATGAGCAGCAGTAACCCAGACCTC GCGGGGACACACTCCGCAGCAGACGAGGAAGTGAAGAACATCATGTCTTCAAAGATCGCCGATCGCAACT GCAGCCGAATGTCTTACTATTGCTCTGGCAGTAGTGATGCTCCAAGTTCACCTGCAGCCCCAAGGCCAGC CAGCAAAAAGCATTTCCATGAGGAGCTTGCCCTTCAGATGGTGGTCAGCACCGGAATGGTGAGAGAAACA GTCTTCAAGTATGCCTGGTTCTTCTTTGAGCTTCTGGTGAAAAGCATGGCCCAGCACGTACATAACATGG ACAAACGGGACAGTTTTCGGAGGACTCGTTTTTCTGACCGTTTCATGGATGACATAACTACTATTGTTAA TGTGGTCACCTCGGAAATTGCAGCCCTTTTAGTAAAACCACAGAAGGAAAATGAACAGGCGGAAAAGATG AACATCAGCCTGGCTTTCTTCTTGTATGACCTTCTCTCCCTCATGGATCGGGGCTTTGTGTTTAACCTCA TCAGACATTATTGCAGCCAGCTGTCAGCCAAGCTCAGTAACCTTCCAACGCTCATTTCCATGAGGCTAGA GTTCCTGAGAATCCTCTGTAGCCATGAGCATTACCTCAATCTGAACCTTTTTTTTATGAATGCTGATACT GCTCCAACATCTCCTTGTCCTTCCATATCTTCCCAGAACTCAAGCTCCTGCTCCAGCTTCCAGGACCAGA AGATCGCCAGCATGTTCGATCTGACTTCCGAGTACCGCCAGCAGCACTTCCTCACCGGGCTCCTCTTCAC AGAACTGGCTGCTGCCCTGGATGCCGAAGGGGAAGGAATCAGCAAAGTACAAAGGAAAGCTGTCAGTGCA ATTCACAGCCTGCTAAGTTCTCACGACCTGGACCCACGCTGTGTCAAACCAGAGGTGAAGGTCAAAATCG CCGCCCTTTACCTACCTTTAGTTGGCATCATTTTGGATGCTTTGCCACAGCTCTGTGACTTTACAGTTGC AGATACTCGCAGATACCGCACCAGTGGCTCGGATGAAGAACAAGAAGGAGCCGGTGCCATTAACCAGAAT GTGGCTCTGGCCATAGCAGGGAATAATTTCAATTTGAAAACAAGTGGAATAGTGCTGTCTTCCTTGCCCT ATAAGCAGTACAACATGCTGAACGCGGACACTACTCGCAACCTCATGATCTGCTTCCTCTGGATCATGAA AAATGCTGATCAGAGCCTCATTAGGAAGTGGATTGCTGACCTGCCATCAACGCAGCTCAACAGGATTTTA GATCTACTTTTCATCTGTGTGTTATGTTTTGAGTATAAGGGAAAACAGAGTTCTGACAAAGTCAGTACCC AAGTCCTGCAGAAGTCAAGGGATGTCAAGGCCCGGCTGGAAGAGGCTTTGCTCCGTGGGGAAGGGGCCAG AGGGGAGATGATGCGCCGCCGGGCTCCAGGGAACGACCGATTTCCAGGCCTAAATGAAAATTTGAGATGG AAGAAAGAGCAGACACATTGGCGGCAAGCTAATGAGAAGCTAGATAAAACAAAGGCCGAGTTAGATCAAG AAGCCTTGATCAGTGGCAATCTGGCTACAGAAGCACATTTAATCATCCTGGATATGCAGGAAAACATTAT CCAGGCGAGCTCGGCTCTGGACTGTAAAGACAGCCTGCTGGGAGGTGTTCTGAGGGTGCTGGTGAATTCT CTGAACTGTGATCAGAGTACCACCTACCTGACTCACTGCTTTGCAACACTCCGTGCTCTCATCGCCAAGT TTGGAGACTTACTCTTCGAAGAGGAGGTGGAACAGTGTTTCGACCTATGTCACCAAGTCCTGCACCACTG CAGCAGCAGCATGGATGTCACCCGGAGCCAAGCCTGTGCCACCCTTTACCTCCTCATGAGGTTCAGTTTT GGAGCCACCAGTAATTTTGCAAGAGTAAAGATGCAAGTAACCATGTCCCTGGCATCTTTGGTGGGAAGAG CACCAGACTTTAATGAAGAGCACCTGAGAAGATCCTTGAGGACAATTTTGGCCTATTCAGAAGAGGACAC AGCCATGCAGATGACTCCTTTTCCCACCCAGGTGGAGGAACTTCTCTGTAATCTGAATAGCATCTTATAT GACACAGTGAAAATGAGGGAATTTCAGGAAGATCCTGAGATGCTTATGGATCTCATGTACAGAATTGCCA AGAGTTACCAGGCATCTCCTGATCTGCGGCTGACCTGGCTCCAGAACATGGCAGAGAAACACACCAAGAA GAAGTGCTACACGGAGGCTGCCATGTGCCTGGTGCACGCCGCTGCGTTAGTGGCTGAGTATCTGAGCATG CTGGAGGACCACAGCTACCTGCCCGTGGGCAGTGTCAGCTTCCAGAATATTTCTTCCAATGTGCTGGAGG AGTCTGTGGTCTCTGAGGACACCCTGTCACCTGACGAGGATGGGGTGTGCGCAGGCCAGTACTTCACCGA GAGTGGCCTGGTAGGCCTCCTGGAGCAGGCCGCGGAGCTCTTCAGCACGGGAGGCTTATATGAGACAGTT AATGAGGTCTACAAGCTGGTCATCCCCATCCTAGAAGCGCATCGAGAATTCCGGAAGCTGACACTCACTC ACAGCAAGCTGCAGAGAGCCTTCGACAGCATCGTTAACAAGGATCATAAGAGAATGTTTGGAACCTACTT CCGAGTTGGTTTCTTTGGATCCAAATTTGGGGATTTGGATGAACAGGAATTTGTCTACAAAGAGCCTGCA ATTACCAAGCTTCCTGAGATCTCACATAGACTAGAGGCATTTTATGGTCAATGTTTTGGTGCAGAATTTG TGGAAGTGATTAAAGACTCCACTCCTGTGGACAAAACCAAGTTGGATCCTAACAAGGCCTACATACAGAT CACTTTTGTGGAGCCCTACTTTGATGAGTATGAGATGAAAGACAGGGTCACATACTTTGAGAAGAATTTC AACCTCCGGAGGTTCATGTACACCACCCCGTTCACCCTGGAGGGGCGGCCTCGGGGAGAGCTGCATGAGC AGTACAGAAGGAACACAGTCCTGACCACTATGCACGCCTTCCCCTTCATCAAGACCAGGATCAGCGTCAT CCAGAAGGAGGAGTTTGTTTTGACACCGATTGAAGTTGCCATTGAAGACATGAAGAAGAAGACCCTGCAG TTAGCAGTTGCCATTAACCAGGAGCCGCCTGATGCAAAGATGCTTCAGATGGTGCTGCAAGGCTCTGTGG GAGCTACTGTAAATCAGGGACCACTGGAAGTAGCCCAAGTGTTTTTGGCTGAAATTCCTGCTGATCCAAA ACTCTATCGACATCACAACAAGTTGAGGTTATGCTTTAAGGAATTCATCATGAGATGTGGTGAAGCTGTA GAGAAAAACAAGCGTCTCATCACGGCAGACCAGAGGGAATATCAGCAGGAACTCAAAAAGAACTATAACA AGCTAAAAGAGAACCTCAGGCCAATGATCGAGCGGAAAATTCCAGAACTGTACAAGCCAATATTCAGAGT TGAGAGTCAAAAGAGGGACTCCTTCCACAGATCTAGTTTCAGGAAATGTGAAACCCAGTTGTCACAGGGC AGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC222789 representing NM_203447
Red=Cloning site Green=Tags(s) MTHLNSLDVQLAQELGDFTDDDLDVVFTPKECRTLQPSLPEEGVELDPHVRDCVQTYIREWLIVNRKNQG SPEICGFKKTGSRKDFHKTLPKQTFESETLECSEPAAQAGPRHLNVLCDVSGKGPVTACDFDLRSLQPDK RLENLLQQVSAEDFEKQNEEARRTNRQAELFALYPSVDEEDAVEIRPVPECPKEHLGNRILVKLLTLKFE IEIEPLFASIALYDVKERKKISENFHCDLNSDQFKGFLRAHTPSVAASSQARSAVFSVTYPSSDIYLVVK IEKVLQQGEIGDCAEPYTVIKESDGGKSKEKIEKLKLQAESFCQRLGKYRMPFAWAPISLSSFFSVSTLE REVTDVDSVVGRSSVGERRTLAQSRRLSERALSLEENGVGSNFKTSTLSVSIFFKQEGDRLSDEDLFKFL ADYKRSSSLQRRVKSIPGLLRLEISTAPEIINCCLTPEMLPVKPFPENRTRPHKEILEFPTREVYVPHTV YRNLLYVYPQRLNFVNKLASARNITIKIQFMCGEDASNAMPVIFGKSSGPEFLQEVYTAVTYHNKSPDFY EEVKIKLPAKLTVNHHLLFTFYHISCQQKQGASVETLLGYSWLPILLNERLQTGSYCLPVALEKLPPNYS MHSAEKVPLQNPPIKWAEGHKGVFNIEVQAVSSVHTQDNHLEKFFTLCHSLESQVTFPIRVLDQKISEMA LEHELKLSIICLNSSRLEPLVLFLHLVLDKLFQLSVQPMVIAGQTANFSQFAFESVVAIANSLHNSKDLS KDQHGRNCLLASYVHYVFRLPEVQRDVPKSDAPTALLDPRSYHTYGRTSAAAVSSKLLQARVMSSSNPDL AGTHSAADEEVKNIMSSKIADRNCSRMSYYCSGSSDAPSSPAAPRPASKKHFHEELALQMVVSTGMVRET VFKYAWFFFELLVKSMAQHVHNMDKRDSFRRTRFSDRFMDDITTIVNVVTSEIAALLVKPQKENEQAEKM NISLAFFLYDLLSLMDRGFVFNLIRHYCSQLSAKLSNLPTLISMRLEFLRILCSHEHYLNLNLFFMNADT APTSPCPSISSQNSSSCSSFQDQKIASMFDLTSEYRQQHFLTGLLFTELAAALDAEGEGISKVQRKAVSA IHSLLSSHDLDPRCVKPEVKVKIAALYLPLVGIILDALPQLCDFTVADTRRYRTSGSDEEQEGAGAINQN VALAIAGNNFNLKTSGIVLSSLPYKQYNMLNADTTRNLMICFLWIMKNADQSLIRKWIADLPSTQLNRIL DLLFICVLCFEYKGKQSSDKVSTQVLQKSRDVKARLEEALLRGEGARGEMMRRRAPGNDRFPGLNENLRW KKEQTHWRQANEKLDKTKAELDQEALISGNLATEAHLIILDMQENIIQASSALDCKDSLLGGVLRVLVNS LNCDQSTTYLTHCFATLRALIAKFGDLLFEEEVEQCFDLCHQVLHHCSSSMDVTRSQACATLYLLMRFSF GATSNFARVKMQVTMSLASLVGRAPDFNEEHLRRSLRTILAYSEEDTAMQMTPFPTQVEELLCNLNSILY DTVKMREFQEDPEMLMDLMYRIAKSYQASPDLRLTWLQNMAEKHTKKKCYTEAAMCLVHAAALVAEYLSM LEDHSYLPVGSVSFQNISSNVLEESVVSEDTLSPDEDGVCAGQYFTESGLVGLLEQAAELFSTGGLYETV NEVYKLVIPILEAHREFRKLTLTHSKLQRAFDSIVNKDHKRMFGTYFRVGFFGSKFGDLDEQEFVYKEPA ITKLPEISHRLEAFYGQCFGAEFVEVIKDSTPVDKTKLDPNKAYIQITFVEPYFDEYEMKDRVTYFEKNF NLRRFMYTTPFTLEGRPRGELHEQYRRNTVLTTMHAFPFIKTRISVIQKEEFVLTPIEVAIEDMKKKTLQ LAVAINQEPPDAKMLQMVLQGSVGATVNQGPLEVAQVFLAEIPADPKLYRHHNKLRLCFKEFIMRCGEAV EKNKRLITADQREYQQELKKNYNKLKENLRPMIERKIPELYKPIFRVESQKRDSFHRSSFRKCETQLSQG S TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_203447 |
| ORF Size | 6093 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_203447.1, NP_982272.1 |
| RefSeq Size | 7257 bp |
| RefSeq ORF | 6300 bp |
| Locus ID | 81704 |
| UniProt ID | Q8NF50 |
| MW | 238.3 kDa |
| Gene Summary | This gene encodes a member of the DOCK180 family of guanine nucleotide exchange factors. Guanine nucleotide exchange factors interact with Rho GTPases and are components of intracellular signaling networks. Mutations in this gene result in the autosomal recessive form of the hyper-IgE syndrome. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Jun 2010] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Dedicator of cytokinesis 8 regulates signal transducer and activator of transcription 3 activation and promotes TH17 cell differentiation
,Keles, S;Charbonnier, LM;Kabaleeswaran, V;Reisli, I;Genel, F;Gulez, N;Al-Herz, W;Ramesh, N;Perez-Atayde, A;Eeder, NK;Kutukculer, N;Wu, H;Geha, RS;Chatila, TA;,
J. Allergy Clin. Immunol.
,PubMed ID 27350570
[DOCK8]
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Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222789L1 | Lenti ORF clone of Human dedicator of cytokinesis 8 (DOCK8), transcript variant 1, Myc-DDK-tagged |
CNY 22,512.00 |
|
| RC229462 | DOCK8 (Myc-DDK-tagged)-Human dedicator of cytokinesis 8 (DOCK8), transcript variant 1 |
CNY 18,990.00 |
|
| RG222789 | DOCK8 (tGFP-tagged) - Human dedicator of cytokinesis 8 (DOCK8), transcript variant 1 |
CNY 20,900.00 |
|
| RG229462 | DOCK8 (tGFP-tagged) - Human dedicator of cytokinesis 8 (DOCK8), transcript variant 1 |
CNY 23,768.00 |
|
| SC308184 | DOCK8 (untagged)-Human dedicator of cytokinesis 8 (DOCK8), transcript variant 1 |
CNY 40,380.00 |
|
| SC328097 | DOCK8 (untagged)-Human dedicator of cytokinesis 8 (DOCK8) |
CNY 40,380.00 |
