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DOCK8 (NM_203447) Human Untagged Clone
CAT＃: SC308184
DOCK8 (untagged)-Human dedicator of cytokinesis 8 (DOCK8), transcript variant 1
CNY 40,380.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HEL-205; MRD2; ZIR8 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC308184 representing NM_203447.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCCACTCTGCCGAGCGCAGAGCGCCGCGCGTTCGCGCTCAAGATCAACAGGTATTCTTCAGCGGAA ATAAGGAAACAGTTTACTCTCCCACCAAACCTTGGCCAGTACCATCGACAGAGCATAAGTACCTCTGGC TTCCCCTCTCTTCAACTACCTCAGTTTTATGACCCTGTGGAGCCAGTGGACTTTGAAGGACTTCTGATG ACACACCTGAACAGCCTGGATGTGCAGCTTGCCCAGGAGCTCGGGGACTTCACTGATGACGACTTGGAC GTGGTGTTCACGCCAAAGGAATGTAGGACTTTGCAGCCCTCTTTGCCGGAGGAAGGGGTTGAACTGGAC CCTCATGTCAGGGACTGTGTTCAGACCTACATCCGTGAGTGGCTAATCGTGAACCGGAAAAACCAAGGA AGTCCAGAAATCTGTGGCTTTAAAAAGACTGGATCTCGAAAAGATTTTCACAAGACGCTTCCGAAACAG ACGTTTGAGTCGGAAACCTTGGAGTGCAGTGAACCCGCTGCTCAGGCAGGCCCCCGCCACTTAAACGTG CTGTGCGACGTGTCTGGGAAAGGCCCCGTCACTGCCTGTGACTTTGACCTCCGCAGCCTGCAGCCTGAC AAGCGGCTAGAAAACCTCCTGCAGCAAGTGAGTGCCGAGGACTTTGAGAAGCAGAACGAGGAGGCCCGG AGGACCAATAGGCAGGCCGAGCTCTTTGCCCTTTACCCATCAGTGGACGAGGAGGATGCTGTGGAAATA CGTCCAGTACCAGAATGTCCCAAGGAACACCTGGGCAACAGAATATTGGTCAAGTTGCTGACCTTGAAG TTCGAGATTGAAATTGAGCCCCTGTTTGCCAGCATTGCCCTCTACGATGTTAAAGAAAGGAAAAAGATC TCAGAAAATTTTCACTGTGACCTGAACTCTGACCAGTTCAAAGGATTTCTGCGAGCTCACACGCCTTCA GTGGCCGCATCAAGTCAGGCGAGATCTGCAGTCTTCTCAGTCACCTACCCGTCCTCAGACATCTACCTG GTAGTCAAGATTGAAAAAGTCCTGCAGCAGGGAGAGATTGGAGACTGTGCAGAGCCCTACACGGTTATC AAAGAAAGTGATGGTGGAAAGAGTAAAGAAAAGATTGAAAAACTAAAACTCCAAGCTGAATCCTTCTGC CAGCGTTTGGGGAAATACCGGATGCCCTTTGCCTGGGCACCCATAAGCTTATCAAGCTTCTTCAATGTC TCCACCCTTGAGAGGGAGGTAACTGATGTGGACTCTGTGGTTGGGAGAAGCTCAGTGGGTGAACGGAGG ACATTGGCCCAATCTAGAAGGCTTTCTGAAAGAGCCCTCTCCTTGGAGGAAAATGGGGTTGGATCCAAC TTCAAAACCTCCACTCTGAGCGTTAGCAGCTTTTTCAAGCAGGAAGGAGATCGCCTTAGCGATGAAGAC TTATTCAAGTTTTTAGCTGACTACAAAAGATCATCATCCTTACAGAGACGAGTCAAGTCAATTCCAGGC TTGCTAAGACTGGAGATTTCTACAGCTCCAGAGATCATCAATTGCTGTCTGACTCCTGAAATGCTGCCC GTGAAACCCTTTCCTGAAAACCGGACACGCCCGCACAAAGAGATTTTGGAATTTCCAACACGAGAAGTA TATGTCCCTCACACTGTGTACAGAAACCTTCTCTATGTCTACCCACAGAGGCTGAACTTTGTAAACAAA CTAGCATCAGCCCGGAACATTACAATAAAGATCCAGTTTATGTGTGGAGAAGATGCTAGCAATGCGATG CCGGTCATCTTTGGAAAATCCAGCGGGCCTGAATTTCTGCAGGAAGTGTACACAGCTGTTACATACCAT AATAAGTCTCCTGACTTTTATGAAGAAGTGAAAATTAAGCTCCCCGCTAAGCTCACAGTAAATCACCAC CTCCTGTTCACCTTCTACCATATCAGCTGTCAGCAGAAGCAAGGAGCCTCCGTGGAAACTCTCCTGGGA TATTCATGGCTGCCAATTCTCTTAAATGAACGTCTTCAAACTGGATCCTACTGTCTCCCAGTTGCCTTG GAAAAATTGCCACCCAACTACTCCATGCATTCTGCTGAGAAAGTCCCATTACAGAATCCTCCCATTAAG TGGGCTGAAGGACATAAGGGAGTATTTAATATTGAAGTGCAAGCTGTTTCTTCTGTACACACCCAGGAC AACCACCTGGAGAAGTTCTTCACCCTCTGCCACTCCCTGGAGAGCCAGGTGACCTTCCCCATCCGCGTG CTGGATCAGAAAATCAGCGAGATGGCGCTGGAGCATGAGCTGAAGCTCAGCATCATCTGCCTGAACTCC TCCCGCCTGGAGCCGCTCGTGCTCTTCCTGCACCTGGTGCTGGACAAGCTCTTCCAGCTGTCCGTGCAG CCCATGGTCATCGCTGGCCAGACAGCCAACTTCTCCCAGTTTGCCTTCGAGTCCGTGGTGGCCATCGCC AACAGTCTGCACAACAGCAAGGACCTGAGCAAGGACCAGCATGGGAGGAACTGCCTGCTGGCTTCCTAC GTGCACTACGTCTTCCGCCTGCCAGAGGTGCAAAGGGATGTGCCCAAGTCAGGCGCTCCCACTGCCCTC CTAGACCCTCGGAGCTACCACACGTATGGCCGCACATCAGCTGCTGCTGTGAGTTCAAAGCTGCTGCAG GCCCGGGTGATGAGCAGCAGTAACCCAGACCTCGCGGGGACACACTCCGCAGCAGACGAGGAAGTGAAG AACATCATGTCTTCAAAGATCGCCGATCGCAACTGCAGCCGAATGTCTTACTATTGCTCTGGCAGTAGT GATGCTCCAAGTTCACCTGCAGCCCCAAGGCCAGCCAGCAAAAAGCATTTCCATGAGGAGCTTGCCCTT CAGATGGTGGTCAGCACCGGAATGGTGAGAGAAACAGTCTTCAAGTATGCCTGGTTCTTCTTTGAGCTT CTGGTGAAAAGCATGGCCCAGCACGTACATAACATGGACAAACGGGACAGTTTTCGGAGGACTCGTTTT TCTGACCGTTTCATGGATGACATAACTACTATTGTTAATGTGGTCACCTCGGAAATTGCAGCCCTTTTA GTAAAACCACAGAAGGAAAATGAACAGGCGGAAAAGATGAACATCAGCCTGGCTTTCTTCTTGTATGAC CTTCTCTCCCTCATGGATCGGGGCTTTGTGTTTAACCTCATCAGACATTATTGCAGCCAGCTGTCAGCC AAGCTCAGTAACCTTCCAACGCTCATTTCCATGAGGCTAGAGTTCCTGAGAATCCTCTGTAGCCATGAG CATTACCTCAATCTGAACCTTTTTTTTATGAATGCTGATACTGCTCCAACATCTCCTTGTCCTTCCATA TCTTCCCAGAACTCAAGCTCCTGCTCCAGCTTCCAGGACCAGAAGATCGCCAGCATGTTCGATCTGACT TCCGAGTACCGCCAGCAGCACTTCCTCACCGGGCTCCTCTTCACAGAACTGGCTGCTGCCCTGGATGCC GAAGGGGAAGGAATCAGCAAAGTACAAAGGAAAGCTGTCAGTGCAATTCACAGCCTGCTAAGTTCTCAC GACCTGGACCCACGCTGTGTCAAACCAGAGGTGAAGGTCAAAATCGCCGCCCTTTACCTACCTTTAGTT GGCATCATTTTGGATGCTTTGCCACAGCTCTGTGACTTTACAGTTGCAGATACTCGCAGATACCGCACC AGTGGCTCGGATGAAGAACAAGAAGGAGCCGGTGCCATTAACCAGAATGTGGCTCTGGCCATAGCAGGG AATAATTTCAATTTGAAAACAAGTGGAATAGTGCTGTCTTCCTTGCCCTATAAGCAGTACAACATGCTG AACGCGGACACTACTCGCAACCTCATGATCTGCTTCCTCTGGATCATGAAAAATGCTGATCAGAGCCTC ATTAGGAAGTGGATTGCTGACCTGCCATCAACGCAGCTCAACAGGATTTTAGATCTACTTTTCATCTGT GTGTTATGTTTTGAGTATAAGGGAAAACAGAGTTCTGACAAAGTCAGTACCCAAGTCCTGCAGAAGTCA AGGGATGTCAAGGCCCGGCTGGAAGAGGCTTTGCTCCGTGGGGAAGGGGCCAGAGGGGAGATGATGCGC CGCCGGGCTCCAGGGAACGACCGATTTCCAGGCCTAAATGAAAATTTGAGATGGAAGAAAGAGCAGACA CATTGGCGGCAAGCTAATGAGAAGCTAGATAAAACAAAGGCCGAGTTAGATCAAGAAGCCTTGATCAGT GGCAATCTGGCTACAGAAGCACATTTAATCATCCTGGATATGCAGGAAAACATTATCCAGGCGAGCTCG GCTCTGGACTGTAAAGACAGCCTGCTGGGAGGTGTTCTGAGGGTGCTGGTGAATTCTCTGAACTGTGAT CAGAGTACCACCTACCTGACTCACTGCTTTGCAACACTCCGTGCTCTCATCGCCAAGTTTGGAGACTTA CTCTTCGAAGAGGAGGTGGAACAGTGTTTCGACCTATGTCACCAAGTCCTGCACCACTGCAGCAGCAGC ATGGATGTCACCCGGAGCCAAGCCTGTGCCACCCTTTACCTCCTCATGAGGTTCAGTTTTGGAGCCACC AGTAATTTTGCAAGAGTAAAGATGCAAGTAACCATGTCCCTGGCATCTTTGGTGGGAAGAGCACCAGAC TTTAATGAAGAGCACCTGAGAAGATCCTTGAGGACAATTTTGGCCTATTCAGAAGAGGACACAGCCATG CAGATGACTCCTTTTCCCACCCAGGTGGAGGAACTTCTCTGTAATCTGAATAGCATCTTATATGACACA GTGAAAATGAGGGAATTTCAGGAAGATCCTGAGATGCTTATGGATCTCATGTACAGAATTGCCAAGAGT TACCAGGCATCTCCTGATCTGCGGCTGACCTGGCTCCAGAACATGGCAGAGAAACACACCAAGAAGAAG TGCTACACGGAGGCTGCCATGTGCCTGGTGCACGCCGCTGCGTTAGTGGCTGAGTATCTGAGCATGCTG GAGGACCACAGCTACCTGCCCGTGGGCAGTGTCAGCTTCCAGAATATTTCTTCCAATGTGCTGGAGGAG TCTGTGGTCTCTGAGGACACCCTGTCACCTGACGAGGATGGGGTGTGCGCAGGCCAGTACTTCACCGAG AGTGGCCTGGTAGGCCTCCTGGAGCAGGCCGCGGAGCTCTTCAGCACGGGAGGCTTATATGAGACAGTT AATGAGGTCTACAAGCTGGTCATCCCCATCCTAGAAGCGCATCGAGAATTCCGGAAGCTGACACTCACT CACAGCAAGCTGCAGAGAGCCTTCGACAGCATCGTTAACAAGGATCATAAGAGAATGTTTGGAACCTAC TTCCGAGTTGGTTTCTTTGGATCCAAATTTGGGGATTTGGATGAACAGGAGTTTGTCTACAAAGAGCCT GCAATTACCAAGCTTCCTGAGATCTCACATAGACTAGAGGCATTTTATGGTCAATGTTTTGGTGCAGAA TTTGTGGAAGTGATTAAAGACTCCACTCCTGTGGACAAAACCAAGTTGGATCCTAACAAGGCCTACATA CAGATCACTTTTGTGGAGCCCTACTTTGATGAGTATGAGATGAAAGACAGGGTCACATACTTTGAGAAG AATTTCAACCTCCGGAGGTTCATGTACACCACCCCGTTCACCCTGGAGGGGCGGCCTCGGGGAGAGCTG CATGAGCAGTACAGAAGGAACACAGTCCTGACCACTATGCACGCCTTCCCCTACATCAAGACCAGGATC AGCGTCATCCAGAAGGAGGAGTTTGTTTTGACACCGATTGAAGTTGCCATTGAAGACATGAAGAAGAAG ACCCTGCAGTTAGCAGTTGCCATTAACCAGGAGCCGCCTGATGCAAAGATGCTTCAGATGGTGCTGCAA GGCTCTGTGGGAGCTACTGTAAATCAGGGACCACTGGAAGTAGCCCAAGTGTTTTTGGCTGAAATTCCT GCTGATCCAAAACTCTATCGACATCACAACAAGTTGAGGTTATGCTTTAAGGAATTCATCATGAGATGT GGTGAAGCTGTAGAGAAAAACAAGCGTCTCATCACGGCAGACCAGAGGGAATATCAGCAGGAACTCAAA AAGAACTATAACAAGCTAAAAGAGAACCTCAGGCCAATGATCGAGCGGAAAATTCCAGAACTGTACAAG CCAATATTCAGAGTTGAGAGTCAAAAGAGGGACTCCTTCCACAGATCTAGTTTCAGGAAATGTGAAACC CAGTTGTCACAGGGCAGCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_203447 |
| Insert Size | 6300 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_203447.3 |
| RefSeq Size | 7470 bp |
| RefSeq ORF | 6300 bp |
| Locus ID | 81704 |
| UniProt ID | Q8NF50 |
| MW | 238.5 kDa |
| Gene Summary | This gene encodes a member of the DOCK180 family of guanine nucleotide exchange factors. Guanine nucleotide exchange factors interact with Rho GTPases and are components of intracellular signaling networks. Mutations in this gene result in the autosomal recessive form of the hyper-IgE syndrome. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Jun 2010] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222789 | DOCK8 (Myc-DDK-tagged)-Human dedicator of cytokinesis 8 (DOCK8), transcript variant 1 |
CNY 20,112.00 |
|
| RC222789L1 | Lenti ORF clone of Human dedicator of cytokinesis 8 (DOCK8), transcript variant 1, Myc-DDK-tagged |
CNY 22,512.00 |
|
| RC229462 | DOCK8 (Myc-DDK-tagged)-Human dedicator of cytokinesis 8 (DOCK8), transcript variant 1 |
CNY 18,990.00 |
|
| RG222789 | DOCK8 (tGFP-tagged) - Human dedicator of cytokinesis 8 (DOCK8), transcript variant 1 |
CNY 20,900.00 |
|
| RG229462 | DOCK8 (tGFP-tagged) - Human dedicator of cytokinesis 8 (DOCK8), transcript variant 1 |
CNY 23,768.00 |
|
| SC328097 | DOCK8 (untagged)-Human dedicator of cytokinesis 8 (DOCK8) |
CNY 40,380.00 |
