SR1 (SRI) (NM_198901) Human Tagged ORF Clone
CAT#: RC222838
SRI (Myc-DDK-tagged)-Human sorcin (SRI), transcript variant 2
ORF Plasmid: tGFP
"NM_198901" in other vectors (6)
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CNY 3,600.00
CNY 4,180.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | CP-22; CP22; SCN; V19 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC222838 representing NM_198901
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGTATGGAGGGGCTCCCGGAGGGCCTGCGTTTCCCGGACAAACTCAGGATCCGCTGTATGGTTACT TTGCTGCTGTAGCTGGACAGGATGGGCAGATAGATGCTGATGAATTGCAGAGATGTCTGACACAGTCTGG CATTGCTGGAGGATACAAACCTTTTAACCTGGAGACTTGCCGGCTTATGGTTTCAATGCTGGATAGAGAT ATGTCTGGCACAATGGGTTTCAATGAATTTAAAGAACTCTGGGCTGTACTGAATGGCTGGAGACAACACT TTATCAGTTTTGACACTGACAGGAGTGGAACAGTAGACCCACAAGAATTGCAGAAGGCCCTGACAACAAT GGGATTTAGGTTGAGTCCCCAGGCTGTGAATTCAATTGCAAAACGATACAGCACCAATGGAAAGATCACC TTCGACGACTACATCGCCTGCTGCGTCAAACTGAGGGCTCTTACAGACAGCTTTCGAAGACGGGATACTG CTCAGCAAGGTGTTGTGAATTTCCCATATGATGATTTCATTCAATGTGTCATGAGTGTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC222838 representing NM_198901
Red=Cloning site Green=Tags(s) MQYGGAPGGPAFPGQTQDPLYGYFAAVAGQDGQIDADELQRCLTQSGIAGGYKPFNLETCRLMVSMLDRD MSGTMGFNEFKELWAVLNGWRQHFISFDTDRSGTVDPQELQKALTTMGFRLSPQAVNSIAKRYSTNGKIT FDDYIACCVKLRALTDSFRRRDTAQQGVVNFPYDDFIQCVMSV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_198901 |
ORF Size | 549 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_198901.1, NP_944490.1 |
RefSeq Size | 2012 bp |
RefSeq ORF | 552 bp |
Locus ID | 6717 |
UniProt ID | P30626 |
Protein Families | Druggable Genome |
MW | 20.2 kDa |
Gene Summary | This gene encodes a calcium-binding protein with multiple E-F hand domains that relocates from the cytoplasm to the sarcoplasmic reticulum in response to elevated calcium levels. In addition to regulating intracellular calcium homeostasis it also modulates excitation-contraction coupling in the heart. Alternative splicing results in multiple transcript variants encoding distinct proteins. Multiple pseudogenes exist for this gene. [provided by RefSeq, Mar 2012] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC222838L1 | Lenti ORF clone of Human sorcin (SRI), transcript variant 2, Myc-DDK-tagged |
CNY 6,000.00 |
|
RC222838L2 | Lenti ORF clone of Human sorcin (SRI), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
RC222838L3 | Lenti ORF clone of Human sorcin (SRI), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC222838L4 | Lenti ORF clone of Human sorcin (SRI), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
RG222838 | SRI (tGFP-tagged) - Human sorcin (SRI), transcript variant 2 |
CNY 5,200.00 |
|
SC307807 | SRI (untagged)-Human sorcin (SRI), transcript variant 2 |
CNY 2,400.00 |