SR1 (SRI) (NM_198901) Human Tagged ORF Clone

CAT#: RC222838

SRI (Myc-DDK-tagged)-Human sorcin (SRI), transcript variant 2

ORF Plasmid: DDK tGFP



  "NM_198901" in other vectors (6)

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CNY 3,600.00

CNY 4,180.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


SRI Rabbit polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms CP-22; CP22; SCN; V19
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC222838 representing NM_198901
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGTATGGAGGGGCTCCCGGAGGGCCTGCGTTTCCCGGACAAACTCAGGATCCGCTGTATGGTTACT
TTGCTGCTGTAGCTGGACAGGATGGGCAGATAGATGCTGATGAATTGCAGAGATGTCTGACACAGTCTGG
CATTGCTGGAGGATACAAACCTTTTAACCTGGAGACTTGCCGGCTTATGGTTTCAATGCTGGATAGAGAT
ATGTCTGGCACAATGGGTTTCAATGAATTTAAAGAACTCTGGGCTGTACTGAATGGCTGGAGACAACACT
TTATCAGTTTTGACACTGACAGGAGTGGAACAGTAGACCCACAAGAATTGCAGAAGGCCCTGACAACAAT
GGGATTTAGGTTGAGTCCCCAGGCTGTGAATTCAATTGCAAAACGATACAGCACCAATGGAAAGATCACC
TTCGACGACTACATCGCCTGCTGCGTCAAACTGAGGGCTCTTACAGACAGCTTTCGAAGACGGGATACTG
CTCAGCAAGGTGTTGTGAATTTCCCATATGATGATTTCATTCAATGTGTCATGAGTGTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC222838 representing NM_198901
Red=Cloning site Green=Tags(s)

MQYGGAPGGPAFPGQTQDPLYGYFAAVAGQDGQIDADELQRCLTQSGIAGGYKPFNLETCRLMVSMLDRD
MSGTMGFNEFKELWAVLNGWRQHFISFDTDRSGTVDPQELQKALTTMGFRLSPQAVNSIAKRYSTNGKIT
FDDYIACCVKLRALTDSFRRRDTAQQGVVNFPYDDFIQCVMSV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_198901
ORF Size 549 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_198901.1, NP_944490.1
RefSeq Size 2012 bp
RefSeq ORF 552 bp
Locus ID 6717
UniProt ID P30626
Protein Families Druggable Genome
MW 20.2 kDa
Gene Summary This gene encodes a calcium-binding protein with multiple E-F hand domains that relocates from the cytoplasm to the sarcoplasmic reticulum in response to elevated calcium levels. In addition to regulating intracellular calcium homeostasis it also modulates excitation-contraction coupling in the heart. Alternative splicing results in multiple transcript variants encoding distinct proteins. Multiple pseudogenes exist for this gene. [provided by RefSeq, Mar 2012]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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