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Cytochrome P450 2A6 (CYP2A6) (NM_000762) Human Tagged ORF Clone
CAT＃: RC222995
CYP2A6 (Myc-DDK-tagged)-Human cytochrome P450, family 2, subfamily A, polypeptide 6 (CYP2A6)
ORF Plasmid: tGFP
"NM_000762" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CPA6; CYP2A; CYP2A3; CYPIIA6; P450C2A; P450PB |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC222995 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGCCTCAGGGATGCTTCTGGTGGCCTTGCTGGTCTGCCTGACTGTGATGGTCTTGATGTCTGTTT GGCAGCAGAGGAAGAGCAAGGGGAAGCTGCCTCCGGGACCCACCCCATTGCCCTTCATTGGAAACTACCT GCAACTGAACACAGAGCAGATGTACAACTCCCTCATGAAGATCAGTGAGCGCTATGGCCCCGTGTTCACC ATTCACTTGGGGCCCCGGCGGGTCGTGGTGCTGTGTGGACATGATGCCGTCAGGGAGGCTCTGGTGGACC AGGCTGAGGAGTTCAGCGGGCGAGGCGAGCAAGCCACCTTCGACTGGGTCTTCAAAGGCTATGGCGTGGT ATTCAGCAACGGGGAGCGCGCCAAGCAGCTCCGGCGCTTCTCCATCGCCACCCTGCGGGACTTCGGGGTG GGCAAGCGAGGCATCGAGGAGCGCATCCAGGAGGAGGCGGGCTTCCTCATCGACGCCCTCCGGGGCACTG GCGGCGCCAATATCGATCCCACCTTCTTCCTGAGCCGCACAGTCTCCAATGTCATCAGCTCCATTGTCTT TGGGGACCGCTTTGACTATAAGGACAAAGAGTTCCTGTCACTGTTGCGCATGATGCTAGGAATCTTCCAG TTCACGTCAACCTCCACGGGGCAGCTCTATGAGATGTTCTCTTCGGTGATGAAACACCTGCCAGGACCAC AGCAACAGGCCTTTCAGTTGCTGCAAGGGCTGGAGGACTTCATAGCCAAGAAGGTGGAGCACAACCAGCG TACGCTGGATCCCAATTCCCCACGGGACTTCATTGACTCCTTTCTCATCCGCATGCAGGAGGAGGAGAAG AACCCCAACACGGAGTTCTACTTGAAAAACCTGGTGATGACCACGTTGAACCTCTTCATTGGGGGCACCG AGACCGTCAGCACCACCCTGCGCTATGGCTTCTTGCTGCTCATGAAGCACCCAGAGGTGGAGGCCAAGGT CCATGAGGAGATTGACAGAGTGATCGGCAAGAACCGGCAGCCCAAGTTTGAGGACCGGGCCAAGATGCCC TACATGGAGGCAGTGATCCACGAGATCCAAAGATTTGGAGACGTGATCCCCATGAGTTTGGCCCGCAGAG TCAAAAAGGACACCAAGTTTCGGGATTTCTTCCTCCCTAAGGGCACCGAAGTGTACCCTATGCTGGGCTC TGTGCTGAGAGACCCCAGTTTCTTCTCCAACCCCCAGGACTTCAATCCCCAGCATTTCCTGAATGAGAAG GGGCAGTTTAAGAAGAGTGATGCTTTTGTGCCCTTTTCCATCGGAAAGCGGAACTGTTTCGGAGAAGGCC TGGCCAGAATGGAGCTCTTTCTCTTCTTCACCACCGTCATGCAGAACTTCCGCCTCAAGTCCTCCCAGTC ACCTAAGGACATTGACGTGTCCCCCAAACACGTGGGCTTTGCCACGATCCCACGAAACTACACCATGAGC TTCCTGCCCCGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC222995 protein sequence
Red=Cloning site Green=Tags(s) MLASGMLLVALLVCLTVMVLMSVWQQRKSKGKLPPGPTPLPFIGNYLQLNTEQMYNSLMKISERYGPVFT IHLGPRRVVVLCGHDAVREALVDQAEEFSGRGEQATFDWVFKGYGVVFSNGERAKQLRRFSIATLRDFGV GKRGIEERIQEEAGFLIDALRGTGGANIDPTFFLSRTVSNVISSIVFGDRFDYKDKEFLSLLRMMLGIFQ FTSTSTGQLYEMFSSVMKHLPGPQQQAFQLLQGLEDFIAKKVEHNQRTLDPNSPRDFIDSFLIRMQEEEK NPNTEFYLKNLVMTTLNLFIGGTETVSTTLRYGFLLLMKHPEVEAKVHEEIDRVIGKNRQPKFEDRAKMP YMEAVIHEIQRFGDVIPMSLARRVKKDTKFRDFFLPKGTEVYPMLGSVLRDPSFFSNPQDFNPQHFLNEK GQFKKSDAFVPFSIGKRNCFGEGLARMELFLFFTTVMQNFRLKSSQSPKDIDVSPKHVGFATIPRNYTMS FLPR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000762 |
| ORF Size | 1482 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000762.6 |
| RefSeq Size | 1775 bp |
| RefSeq ORF | 1485 bp |
| Locus ID | 1548 |
| UniProt ID | P11509 |
| Domains | p450 |
| Protein Families | Druggable Genome, P450, Transmembrane |
| Protein Pathways | Caffeine metabolism, Drug metabolism - cytochrome P450, Drug metabolism - other enzymes, Metabolic pathways, Retinol metabolism |
| MW | 56.5 kDa |
| Gene Summary | This gene, CYP2A6, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to hydroxylate coumarin, and also metabolizes nicotine, aflatoxin B1, nitrosamines, and some pharmaceuticals. Individuals with certain allelic variants are said to have a poor metabolizer phenotype, meaning they do not efficiently metabolize coumarin or nicotine. This gene is part of a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q. The gene was formerly referred to as CYP2A3; however, it has been renamed CYP2A6. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Similarities in mRNA expression of peripheral blood drug metabolizing enzymes and cancer marker genes with biopsy samples of head and neck cancer patients
,Hasan, F;Katiyar, T;Maurya, SS;Yadav, V;Yadav, S;Pandey, R;Mehrotra, D;Hadi, R;Singh, S;Bhatt, ML;Parmar, D;,
Biomarkers
,PubMed ID 31002268
[Cytochrome P450 2A6]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222995L3 | Lenti ORF clone of Human cytochrome P450, family 2, subfamily A, polypeptide 6 (CYP2A6), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC222995L4 | Lenti ORF clone of Human cytochrome P450, family 2, subfamily A, polypeptide 6 (CYP2A6), mGFP tagged |
CNY 5,890.00 |
|
| RG222995 | CYP2A6 (tGFP-tagged) - Human cytochrome P450, family 2, subfamily A, polypeptide 6 (CYP2A6) |
CNY 5,256.00 |
|
| SC120818 | CYP2A6 (untagged)-Human cytochrome P450, family 2, subfamily A, polypeptide 6 (CYP2A6) |
CNY 3,656.00 |
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