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TLL1 (NM_012464) Human Tagged ORF Clone
CAT＃: RC223360
TLL1 (Myc-DDK-tagged)-Human tolloid-like 1 (TLL1), transcript variant 1
ORF Plasmid: tGFP
"NM_012464" in other vectors (6)
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CNY 7,056.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ASD6; TLL |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC223360 representing NM_012464
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGTTGGGAACGCTTTCCCCGAGGATGCTCGTGTGGCTGGTGGCCTCGGGGATTGTTTTCTACGGGG AGCTATGGGTCTGCGCTGGCCTCGATTATGATTACACTTTTGATGGGAACGAAGAGGATAAAACAGAGAC TATAGATTACAAGGACCCGTGTAAAGCCGCTGTATTTTGGGGCGATATTGCCTTAGATGATGAAGACTTA AATATCTTTCAAATAGATAGGACAATTGACCTTACGCAGAACCCCTTTGGAAACCTTGGACATACCACAG GTGGACTTGGAGACCATGCTATGTCAAAGAAGCGAGGGGCCCTCTACCAACTTATAGACAGGATAAGAAG AATTGGCTTTGGCTTGGAGCAAAACAACACAGTTAAGGGAAAAGTACCTCTACAATTCTCAGGGCAAAAT GAGAAAAATCGAGTTCCCAGAGCCGCTACATCAAGAACGGAAAGAATATGGCCTGGAGGCGTTATTCCTT ATGTTATAGGAGGAAACTTCACTGGCAGCCAGAGAGCCATGTTCAAGCAGGCCATGAGGCACTGGGAAAA GCACACATGTGTGACTTTCATAGAAAGAAGTGATGAAGAGAGTTACATTGTATTCACCTATAGGCCTTGT GGATGCTGCTCCTATGTAGGTCGGCGAGGAAATGGACCTCAGGCAATCTCTATCGGCAAGAACTGTGATA AATTTGGGATTGTTGTTCATGAATTGGGTCATGTGATAGGCTTTTGGCATGAACACACAAGACCAGATCG AGATAACCACGTAACTATCATAAGAGAAAACATCCAGCCAGGTCAAGAGTACAATTTTCTGAAGATGGAG CCTGGAGAAGTAAACTCACTTGGAGAAAGATATGATTTCGACAGTATCATGCACTATGCCAGGAACACCT TCTCAAGGGGGATGTTTCTGGATACCATTCTCCCCTCCCGTGATGATAATGGCATACGTCCTGCAATTGG TCAGCGAACCCGTCTAAGCAAAGGAGATATCGCACAGGCAAGAAAGCTGTATAGATGTCCAGCATGTGGA GAAACTCTACAAGAATCCAATGGCAACCTTTCCTCTCCAGGATTTCCCAATGGCTACCCTTCTTACACAC ACTGCATCTGGAGAGTTTCTGTGACCCCAGGGGAGAAGATTGTTTTAAATTTTACAACGATGGATCTATA CAAGAGTAGTTTGTGCTGGTATGACTATATTGAAGTAAGAGACGGGTACTGGAGAAAATCACCTCTCCTT GGTAGATTCTGTGGGGACAAATTGCCTGAAGTTCTTACTTCTACAGACAGCAGAATGTGGATTGAGTTTC GTAGCAGCAGTAATTGGGTAGGAAAAGGCTTTGCAGCTGTCTATGAAGCGATCTGTGGAGGTGAGATACG TAAAAATGAAGGACAGATTCAGTCTCCCAATTATCCTGATGACTATCGCCCGATGAAAGAATGTGTGTGG AAAATAACAGTGTCTGAGAGCTACCACGTCGGGCTGACCTTTCAGTCCTTTGAGATTGAAAGACATGACA ATTGTGCTTATGACTACCTGGAAGTTAGAGATGGAACCAGTGAAAATAGCCCTTTGATAGGGCGTTTCTG TGGTTATGACAAACCTGAAGACATAAGATCTACCTCCAATACTTTGTGGATGAAGTTTGTTTCTGACGGA ACTGTGAACAAAGCAGGGTTTGCTGCTAACTTTTTTAAAGAGGAAGATGAGTGTGCCAAACCTGACCGTG GAGGCTGTGAGCAGCGATGTCTGAACACTCTGGGCAGTTACCAGTGTGCCTGTGAGCCTGGCTATGAGCT GGGCCCAGACAGAAGGAGCTGTGAAGCTGCTTGTGGTGGACTTCTTACCAAACTTAACGGCACCATAACC ACCCCTGGCTGGCCCAAGGAGTACCCTCCTAATAAGAACTGTGTGTGGCAAGTGGTTGCACCAACCCAGT ACAGAATTTCTGTGAAGTTTGAGTTTTTTGAATTGGAAGGCAATGAAGTTTGCAAATATGATTATGTGGA GATCTGGAGTGGTCTTTCCTCTGAGTCTAAACTGCATGGCAAATTCTGTGGCGCTGAAGTGCCTGAAGTG ATCACATCCCAGTTCAACAATATGAGAATTGAATTCAAATCTGACAATACTGTATCCAAGAAGGGCTTCA AAGCACATTTTTTCTCAGACAAAGATGAATGCTCTAAGGATAATGGTGGATGTCAGCACGAATGTGTCAA CACGATGGGGAGCTACATGTGTCAATGCCGTAATGGATTTGTGCTACATGACAATAAACATGATTGCAAG GAAGCTGAGTGTGAACAGAAGATCCACAGTCCAAGTGGCCTCATCACCAGTCCCAACTGGCCAGACAAGT ACCCAAGCAGGAAAGAATGCACTTGGGAAATCAGCGCCACTCCTGGCCACCGAATCAAATTAGCCTTTAG TGAATTTGAGATTGAGCAGCATCAAGAATGTGCTTATGACCACTTAGAAGTATTTGATGGAGAAACAGAA AAGTCACCGATTCTTGGACGACTGTGTGGCAACAAGATACCAGATCCCCTTGTGGCTACTGGAAATAAAA TGTTTGTTCGGTTTGTTTCTGATGCATCTGTTCAAAGAAAAGGCTTTCAAGCTACACATTCTACAGAGTG TGGCGGACGATTGAAAGCAGAATCAAAACCAAGAGATCTGTACTCACATGCTCAGTTTGGTGATAACAAC TACCCAGGACAGGTTGACTGTGAATGGCTATTAGTATCAGAACGGGGCTCTCGACTTGAATTATCCTTCC AGACATTTGAAGTGGAGGAAGAAGCAGACTGTGGCTATGACTATGTGGAGCTCTTTGATGGTCTTGATTC AACAGCTGTGGGGCTTGGTCGATTCTGTGGATCCGGGCCACCAGAAGAGATTTATTCAATTGGAGATTCA GTTTTAATTCATTTCCACACTGATGACACAATCAACAAGAAGGGATTTCATATAAGATACAAAAGCATAA GATATCCAGATACCACACATACCAAAAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC223360 representing NM_012464
Red=Cloning site Green=Tags(s) MGLGTLSPRMLVWLVASGIVFYGELWVCAGLDYDYTFDGNEEDKTETIDYKDPCKAAVFWGDIALDDEDL NIFQIDRTIDLTQNPFGNLGHTTGGLGDHAMSKKRGALYQLIDRIRRIGFGLEQNNTVKGKVPLQFSGQN EKNRVPRAATSRTERIWPGGVIPYVIGGNFTGSQRAMFKQAMRHWEKHTCVTFIERSDEESYIVFTYRPC GCCSYVGRRGNGPQAISIGKNCDKFGIVVHELGHVIGFWHEHTRPDRDNHVTIIRENIQPGQEYNFLKME PGEVNSLGERYDFDSIMHYARNTFSRGMFLDTILPSRDDNGIRPAIGQRTRLSKGDIAQARKLYRCPACG ETLQESNGNLSSPGFPNGYPSYTHCIWRVSVTPGEKIVLNFTTMDLYKSSLCWYDYIEVRDGYWRKSPLL GRFCGDKLPEVLTSTDSRMWIEFRSSSNWVGKGFAAVYEAICGGEIRKNEGQIQSPNYPDDYRPMKECVW KITVSESYHVGLTFQSFEIERHDNCAYDYLEVRDGTSENSPLIGRFCGYDKPEDIRSTSNTLWMKFVSDG TVNKAGFAANFFKEEDECAKPDRGGCEQRCLNTLGSYQCACEPGYELGPDRRSCEAACGGLLTKLNGTIT TPGWPKEYPPNKNCVWQVVAPTQYRISVKFEFFELEGNEVCKYDYVEIWSGLSSESKLHGKFCGAEVPEV ITSQFNNMRIEFKSDNTVSKKGFKAHFFSDKDECSKDNGGCQHECVNTMGSYMCQCRNGFVLHDNKHDCK EAECEQKIHSPSGLITSPNWPDKYPSRKECTWEISATPGHRIKLAFSEFEIEQHQECAYDHLEVFDGETE KSPILGRLCGNKIPDPLVATGNKMFVRFVSDASVQRKGFQATHSTECGGRLKAESKPRDLYSHAQFGDNN YPGQVDCEWLLVSERGSRLELSFQTFEVEEEADCGYDYVELFDGLDSTAVGLGRFCGSGPPEEIYSIGDS VLIHFHTDDTINKKGFHIRYKSIRYPDTTHTKK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012464 |
| ORF Size | 3039 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012464.5 |
| RefSeq Size | 6654 bp |
| RefSeq ORF | 3042 bp |
| Locus ID | 7092 |
| UniProt ID | O43897 |
| Protein Families | Druggable Genome, Protease, Secreted Protein |
| MW | 114.5 kDa |
| Gene Summary | This gene encodes an astacin-like, zinc-dependent, metalloprotease that belongs to the peptidase M12A family. This protease processes procollagen C-propeptides, such as chordin, pro-biglycan and pro-lysyl oxidase. Studies in mice suggest that this gene plays multiple roles in the development of mammalian heart, and is essential for the formation of the interventricular septum. Allelic variants of this gene are associated with atrial septal defect type 6. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The Secreted Protein ANGPTL2 Promotes Metastasis of Osteosarcoma Cells Through Integrin α5β1, p38 MAPK, and Matrix Metalloproteinases
,Odagiri, H;Kadomatsu, T;Endo, M;Masuda, T;Morioka, MS;Fukuhara, S;Miyamoto, T;Kobayashi, E;Miyata, K;Aoi, J;Horiguchi, H;Nishimura, N;Terada, K;Yakushiji, T;Manabe, I;Mochizuki, N;Mizuta, H;Oike, Y;,
Sci Signal, Jan 2014.
,PubMed ID 24448647
[TLL1]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223360L1 | Lenti ORF clone of Human tolloid-like 1 (TLL1), transcript variant 1, Myc-DDK-tagged |
CNY 9,456.00 |
|
| RC223360L2 | Lenti ORF clone of Human tolloid-like 1 (TLL1), transcript variant 1, mGFP tagged |
CNY 7,600.00 |
|
| RC223360L3 | Lenti ORF clone of Human tolloid-like 1 (TLL1), transcript variant 1, Myc-DDK-tagged |
CNY 7,600.00 |
|
| RC223360L4 | Lenti ORF clone of Human tolloid-like 1 (TLL1), transcript variant 1, mGFP tagged |
CNY 9,456.00 |
|
| RG223360 | TLL1 (tGFP-tagged) - Human tolloid-like 1 (TLL1), transcript variant 1 |
CNY 6,270.00 |
|
| SC128122 | TLL1 (untagged)-Human tolloid-like 1 (TLL1), transcript variant 1 |
CNY 7,064.00 |
