United States
Germany
Japan
United Kingdom
China
FBXL11 (KDM2A) (NM_012308) Human Tagged ORF Clone
CAT＃: RC223440
- TrueORF®
KDM2A (Myc-DDK-tagged)-Human lysine (K)-specific demethylase 2A (KDM2A), transcript variant 1
ORF Plasmid: tGFP
"NM_012308" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 8,088.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CXXC8; FBL7; FBL11; FBXL11; JHDM1A; LILINA |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC223440 representing NM_012308
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACCCGAAGAAGAAAGGATTCGTTACAGCCAGAGATTGCGTGGTACCATGCGACGACGCTATGAAG ATGATGGCATTTCAGATGATGAAATTGAAGGAAAAAGAACTTTTGACTTGGAAGAGAAACTGCACACCAA CAAATATAATGCCAATTTTGTTACTTTTATGGAAGGAAAAGATTTTAATGTAGAGTATATTCAGCGGGGT GGCTTGAGAGATCCTCTGATTTTCAAGAATTCTGATGGACTCGGAATAAAAATGCCGGATCCAGACTTCA CTGTGAATGATGTCAAAATGTGTGTGGGGAGTCGTCGCATGGTGGATGTCATGGACGTGAACACACAGAA AGGCATTGAAATGACCATGGCTCAGTGGACACGCTACTATGAGACCCCAGAGGAGGAGCGAGAGAAACTC TATAATGTCATCAGCCTCGAGTTTAGCCACACCAGGCTGGAGAATATGGTGCAGAGGCCCTCCACGGTGG ATTTCATTGACTGGGTAGACAACATGTGGCCAAGGCACTTGAAGGAAAGCCAGACTGAATCAACAAATGC CATCTTGGAGATGCAGTACCCTAAAGTGCAGAAGTACTGTCTAATGAGTGTTCGAGGCTGCTATACTGAC TTCCATGTGGACTTTGGTGGTACCTCTGTTTGGTATCACATCCATCAAGGGGGAAAGGTCTTCTGGCTCA TCCCCCCTACAGCCCACAACCTGGAGCTGTACGAGAATTGGCTGCTGTCAGGGAAACAGGGAGACATCTT TCTGGGTGACCGGGTATCAGATTGTCAGCGCATTGAGCTCAAGCAGGGCTATACCTTCGTCATTCCCTCA GGCTGGATTCATGCTGTGTATACTCCTACAGACACATTAGTGTTTGGGGGCAATTTTTTGCATAGCTTCA ACATCCCTATGCAGTTAAAAATATACAACATTGAAGATCGGACACGGGTTCCAAATAAGTTTCGCTATCC ATTCTACTATGAGATGTGTTGGTATGTGTTGGAGCGCTATGTGTACTGCATAACCAACCGTTCCCACCTA ACCAAGGAATTTCAGAAAGAGTCCCTCAGCATGGATTTGGAGTTAAATGGGTTGGAGTCTGGGAATGGGG ATGAGGAAGCAGTGGATCGAGAACCCCGACGCTTGAGCAGCAGGCGTTCTGTCCTCACTAGCCCTGTAGC GAATGGAGTCAACCTGGATTATGATGGACTGGGCAAAACCTGCCGAAGTCTTCCAAGTCTGAAGAAAACT TTGGCTGGGGACTCATCTTCTGACTGTAGCCGGGGCTCCCACAATGGACAAGTGTGGGATCCCCAGTGTG CTCCCCGAAAGGACAGGCAAGTGCATCTGACCCATTTTGAGCTTGAAGGCCTTCGCTGCCTTGTAGATAA GTTGGAGTCTCTGCCACTGCACAAGAAATGTGTCCCCACAGGGATAGAAGATGAAGATGCTCTCATTGCT GATGTAAAGATTTTGCTGGAGGAGCTTGCCAACAGCGATCCCAAGTTAGCCCTCACTGGAGTTCCTATAG TACAGTGGCCAAAAAGGGATAAGCTTAAATTCCCCACTCGGCCAAAGGTGCGGGTTCCTACCATCCCCAT TACGAAGCCTCACACTATGAAACCAGCTCCACGGTTAACACCTGTGAGGCCAGCTGCTGCCTCCCCGATT GTGTCAGGAGCCAGACGGAGACGAGTGCGATGTCGAAAATGCAAAGCCTGTGTGCAAGGAGAGTGTGGTG TTTGCCACTACTGCAGAGACATGAAGAAGTTTGGGGGGCCTGGACGCATGAAGCAGTCCTGTGTCCTCCG ACAGTGCTTGGCACCCAGACTGCCTCACTCAGTCACATGTTCCCTCTGTGGAGAGGTGGATCAGAATGAA GAGACACAAGACTTTGAGAAGAAACTCATGGAATGCTGTATCTGCAATGAGATTGTTCATCCTGGCTGCC TCCAGATGGACGGAGAGGGGTTGCTTAACGAAGAATTGCCAAATTGCTGGGAATGTCCAAAGTGCTACCA GGAGGACAGCTCGGAGAAAGCCCAGAAGCGGAAAATGGAAGAGAGTGACGAAGAAGCTGTGCAAGCCAAA GTCCTGCGGCCCCTGCGGAGCTGCGATGAGCCTCTCACGCCCCCGCCTCATTCACCCACTTCCATGCTGC AGCTCATCCATGACCCGGTTTCCCCCCGGGGTATGGTGACTCGGTCATCCCCTGGGGCTGGCCCCAGCGA CCACCACAGTGCCAGCCGCGATGAGCGCTTCAAACGGCGGCAGTTGCTGCGGCTGCAGGCCACAGAGCGC ACCATGGTACGGGAAAAGGAGAACAATCCCAGCGGCAAAAAGGAGCTGTCTGAAGTTGAGAAAGCCAAGA TCCGGGGATCGTACCTCACTGTCACGCTACAGAGGCCCACCAAAGAGCTCCACGGGACATCCATTGTGCC CAAGCTGCAGGCCATCACGGCCTCCTCTGCCAACCTTCGCCATTCCCCCCGTGTGCTAGTGCAGCACTGC CCAGCCCGAACCCCCCAGCGTGGGGATGAGGAGGGGCTGGGGGGAGAGGAGGAGGAAGAGGAGGAGGAGG AGGAGGAAGATGACAGTGCAGAGGAGGGGGGTGCAGCCAGGCTGAATGGCCGGGGCAGTTGGGCTCAGGA TGGAGACGAAAGCTGGATGCAGCGGGAGGTCTGGATGTCTGTCTTCCGCTACCTCAGCCGCAGAGAACTT TGTGAATGTATGCGAGTGTGCAAGACGTGGTATAAATGGTGCTGCGACAAGAGACTTTGGACAAAAATTG ACTTGAGTAGGTGTAAGGCCATTGTGCCCCAGGCCCTCAGTGGCATCATCAAGAGGCAGCCAGTCAGCCT TGACCTCAGTTGGACCAACATCTCTAAAAAGCAACTGACATGGCTCGTCAATAGGCTGCCAGGACTGAAA GACCTCCTCCTAGCAGGCTGCTCCTGGTCTGCAGTCTCTGCCCTCAGCACCTCCAGCTGCCCCCTTCTCA GGACCCTTGATCTTCGGTGGGCAGTAGGAATCAAGGACCCTCAAATTCGGGACTTGCTTACTCCACCGGC TGATAAACCAGGTCAGGACAATCGCAGCAAGCTCCGGAACATGACCGACTTCCGGCTGGCAGGCCTTGAC ATCACAGATGCCACGCTTCGCCTCATAATTCGCCACATGCCCCTCCTGTCTCGACTCGACCTCAGTCACT GCAGCCACCTTACAGATCAGTCCTCCAATCTACTCACTGCTGTCGGGTCTTCCACTCGCTACTCTCTCAC AGAGCTCAATATGGCAGGTTGCAATAAATTGACAGACCAGACCCTGATCTACCTACGGCGCATTGCCAAC GTCACCTTGATCGACCTTCGAGGATGCAAGCAGATCACTCGAAAAGCCTGCGAGCACTTCATCTCAGACT TGTCCATCAACAGCCTCTACTGCCTGTCTGACGAGAAGCTGATACAGAAGATCAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC223440 representing NM_012308
Red=Cloning site Green=Tags(s) MEPEEERIRYSQRLRGTMRRRYEDDGISDDEIEGKRTFDLEEKLHTNKYNANFVTFMEGKDFNVEYIQRG GLRDPLIFKNSDGLGIKMPDPDFTVNDVKMCVGSRRMVDVMDVNTQKGIEMTMAQWTRYYETPEEEREKL YNVISLEFSHTRLENMVQRPSTVDFIDWVDNMWPRHLKESQTESTNAILEMQYPKVQKYCLMSVRGCYTD FHVDFGGTSVWYHIHQGGKVFWLIPPTAHNLELYENWLLSGKQGDIFLGDRVSDCQRIELKQGYTFVIPS GWIHAVYTPTDTLVFGGNFLHSFNIPMQLKIYNIEDRTRVPNKFRYPFYYEMCWYVLERYVYCITNRSHL TKEFQKESLSMDLELNGLESGNGDEEAVDREPRRLSSRRSVLTSPVANGVNLDYDGLGKTCRSLPSLKKT LAGDSSSDCSRGSHNGQVWDPQCAPRKDRQVHLTHFELEGLRCLVDKLESLPLHKKCVPTGIEDEDALIA DVKILLEELANSDPKLALTGVPIVQWPKRDKLKFPTRPKVRVPTIPITKPHTMKPAPRLTPVRPAAASPI VSGARRRRVRCRKCKACVQGECGVCHYCRDMKKFGGPGRMKQSCVLRQCLAPRLPHSVTCSLCGEVDQNE ETQDFEKKLMECCICNEIVHPGCLQMDGEGLLNEELPNCWECPKCYQEDSSEKAQKRKMEESDEEAVQAK VLRPLRSCDEPLTPPPHSPTSMLQLIHDPVSPRGMVTRSSPGAGPSDHHSASRDERFKRRQLLRLQATER TMVREKENNPSGKKELSEVEKAKIRGSYLTVTLQRPTKELHGTSIVPKLQAITASSANLRHSPRVLVQHC PARTPQRGDEEGLGGEEEEEEEEEEEDDSAEEGGAARLNGRGSWAQDGDESWMQREVWMSVFRYLSRREL CECMRVCKTWYKWCCDKRLWTKIDLSRCKAIVPQALSGIIKRQPVSLDLSWTNISKKQLTWLVNRLPGLK DLLLAGCSWSAVSALSTSSCPLLRTLDLRWAVGIKDPQIRDLLTPPADKPGQDNRSKLRNMTDFRLAGLD ITDATLRLIIRHMPLLSRLDLSHCSHLTDQSSNLLTAVGSSTRYSLTELNMAGCNKLTDQTLIYLRRIAN VTLIDLRGCKQITRKACEHFISDLSINSLYCLSDEKLIQKIS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012308 |
| ORF Size | 3486 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012308.3 |
| RefSeq Size | 6210 bp |
| RefSeq ORF | 3489 bp |
| Locus ID | 22992 |
| UniProt ID | Q9Y2K7 |
| Domains | F-box, PHD, zf-CXXC, JmjC, LRR_CC |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 132.6 kDa |
| Gene Summary | This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains at least six highly degenerated leucine-rich repeats. This family member plays a role in epigenetic silencing. It nucleates at CpG islands and specifically demethylates both mono- and di-methylated lysine-36 of histone H3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223440L1 | Lenti ORF clone of Human lysine (K)-specific demethylase 2A (KDM2A), transcript variant 1, Myc-DDK-tagged |
CNY 10,488.00 |
|
| RC223440L2 | Lenti ORF clone of Human lysine (K)-specific demethylase 2A (KDM2A), transcript variant 1, mGFP tagged |
CNY 13,490.00 |
|
| RC223440L3 | Lenti ORF clone of Human lysine (K)-specific demethylase 2A (KDM2A), transcript variant 1, Myc-DDK-tagged |
CNY 10,488.00 |
|
| RC223440L4 | Lenti ORF clone of Human lysine (K)-specific demethylase 2A (KDM2A), transcript variant 1, mGFP tagged |
CNY 10,488.00 |
|
| RG223440 | KDM2A (tGFP-tagged) - Human lysine (K)-specific demethylase 2A (KDM2A), transcript variant 1 |
CNY 9,688.00 |
|
| SC309965 | KDM2A (untagged)-Human lysine (K)-specific demethylase 2A (KDM2A), transcript variant 1 |
CNY 8,096.00 |
