SP100 (NM_003113) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	lysp100b
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC223736 representing NM_003113 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGGTGGGGGCGGCGACCTGAGCACCAGGAGGCTGAATGAATGTATTTCACCAGTAGCAAATGAGA TGAACCATCTTCCTGCACACAGCCACGATTTGCAAAGGATGTTCACGGAAGACCAGGGTGTAGATGACAG GCTGCTCTATGACATTGTATTCAAGCACTTCAAAAGAAATAAGGTGGAGATTTCAAATGCAATAAAAAAG ACATTTCCATTCCTCGAGGGCCTCCGTGATCGTGATCTCATCACAAATAAAATGTTTGAAGATTCTCAAG ATTCTTGTAGAAACCTGGTCCCTGTACAGAGAGTGGTGTACAATGTTCTTAGTGAACTGGAGAAGACATT TAACCTGCCAGTTCTGGAAGCACTGTTCAGCGATGTCAACATGCAGGAATACCCCGATTTAATTCACATT TATAAAGGCTTTGAAAATGTAATCCATGACAAATTGCCTCTCCAAGAAAGTGAAGAAGAAGAGAGGGAGG AGAGGTCTGGCCTCCAACTAAGTCTTGAACAAGGAACTGGTGAAAACTCTTTTCGAAGCCTGACTTGGCC ACCTTCGGGTTCCCCATCTCATGCTGGTACAACCCCACCTGAAAATGGACTCTCAGAGCACCCCTGTGAA ACAGAACAGATAAATGCAAAGAGAAAAGATACAACCAGTGACAAAGATGATTCGCTAGGAAGCCAACAAA CAAATGAACAATGTGCTCAAAAGGCTGAGCCAACAGAGTCCTGCGAACAAATTGCTGTCCAAGTGAATAA TGGGGATGCTGGAAGGGAGATGCCCTGCCCGTTGCCCTGTGATGAAGAAAGCCCAGAGGCAGAGCTACAC AACCATGGAATCCAAATTAATTCCTGTTCTGTGCGACTGGTGGATATAAAAAAGGAAAAGCCATTTTCTA ATTCAAAAGTTGAGTGCCAAGCCCAAGCAAGAACTCATCATAACCAGGCATCTGACATAATAGTCATCAG CAGTGAGGACTCTGAAGGATCCACTGACGTTGATGAGCCCTTAGAAGTCTTCATCTCAGCACCGAGAAGT GAGCCTGTGATCAATAATGACAACCCTTTAGAATCAAATGATGAAAAGGAGGGCCAAGAAGCCACTTGCT CACGACCCCAGATTGTACCAGAGCCCATGGATTTCAGAAAATTATCTACATTCAGAGAAAGTTTTAAGAA AAGAGTGATAGGACAAGACCACGACTTTTCAGAATCCAGTGAGGAGGAGGCGCCCGCAGAAGCCTCGAGC GGGGCACTGAGAAGCAAGCATGGTGAGAAGGCTCCTATGACTTCTAGAAGTACATCTACTTGGAGAATAC CCAGCAGGAAGAGACGTTTCAGCAGTAGTGACTTTTCAGACCTGAGTAATGGAGAAGAGCTTCAGGAAAC CTGCAGCTCATCCCTAAGAAGAGGGTCAGGATCACAGCCACAAGAACCTGAAAATAAGAAGTGCTCCTGT GTCATGTGTTTTCCAAAAGGTGTGCCAAGAAGCCAAGAAGCAAGGACTGAAAGTAGTCAAGCATCTGACA TGATGGATACCATGGATGTTGAAAACAATTCTACTTTGGAAAAACACAGTGGGAAAAGAAGAAAAAAGAG AAGGCATAGATCTAAAGTAAATGGTCTCCAAAGAGGGAGAAAGAAAGACAGACCTAGAAAACATTTAACT CTGAATAACAAAGTCCAAAAGAAAAGATGGCAACAAAGAGGAAGAAAAGCCAACACTAGACCTTTGAAAA GAAGAAGAAAAAGAGGTCCAAGAATTCCCAAAGATGAAAATATTAATTTTAAACAATCTGAACTTCCTGT GACCTGTGGTGAGGTGAAGGGCACTCTATATAAGGAGCGATTCAAACAAGGAACCTCAAAGAAGTGTATA CAGAGTGAGGATAAAAAGTGGTTCACTCCCAGGGAATTTGAAATTGAAGGAGACCGCGGAGCATCCAAGA ACTGGAAGCTAAGTATACGCTGCGGTGGATATACCCTGAAAGTCCTGATGGAGAACAAATTTCTGCCAGA ACCACCAAGCACAAGAAAAAAGAGAATACTGGAATCTCACAACAATACCTTAGTTGACCCTTGTGAGGAG CATAAGAAGAAGAACCCAGATGCTTCAGTCAAGTTCTCAGAGTTTTTAAAGAAGTGCTCAGAGACATGGA AGACCATTTTTGCTAAAGAGAAAGGAAAATTTGAAGATATGGCAAAGGCGGACAAGGCCCATTATGAAAG AGAAATGAAAACCTATATCCCTCCTAAAGGGGAGAAAAAAAAGAAGTTCAAGGATCCCAATGCACCCAAG AGGCCTCCTTTGGCCTTTTTCCTGTTCTGCTCTGAGTATCGCCCAAAAATCAAAGGAGAACATCCTGGCC TGTCCATTGATGATGTTGTGAAGAAACTGGCAGGGATGTGGAATAACACCGCTGCAGCTGACAAGCAGTT TTATGAAAAGAAGGCTGCAAAGCTGAAGGAAAAATACAAAAAGGATATTGCTGCATATCGAGCTAAAGGA AAGCCTAATTCAGCAAAAAAGAGAGTTGTCAAGGCTGAAAAAAGCAAGAAAAAGAAGGAAGAGGAAGAAG ATGAAGAGGATGAACAAGAGGAGGAAAATGAAGAAGATGATGATAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC223736 representing NM_003113 Red=Cloning site Green=Tags(s) MAGGGGDLSTRRLNECISPVANEMNHLPAHSHDLQRMFTEDQGVDDRLLYDIVFKHFKRNKVEISNAIKK TFPFLEGLRDRDLITNKMFEDSQDSCRNLVPVQRVVYNVLSELEKTFNLPVLEALFSDVNMQEYPDLIHI YKGFENVIHDKLPLQESEEEEREERSGLQLSLEQGTGENSFRSLTWPPSGSPSHAGTTPPENGLSEHPCE TEQINAKRKDTTSDKDDSLGSQQTNEQCAQKAEPTESCEQIAVQVNNGDAGREMPCPLPCDEESPEAELH NHGIQINSCSVRLVDIKKEKPFSNSKVECQAQARTHHNQASDIIVISSEDSEGSTDVDEPLEVFISAPRS EPVINNDNPLESNDEKEGQEATCSRPQIVPEPMDFRKLSTFRESFKKRVIGQDHDFSESSEEEAPAEASS GALRSKHGEKAPMTSRSTSTWRIPSRKRRFSSSDFSDLSNGEELQETCSSSLRRGSGSQPQEPENKKCSC VMCFPKGVPRSQEARTESSQASDMMDTMDVENNSTLEKHSGKRRKKRRHRSKVNGLQRGRKKDRPRKHLT LNNKVQKKRWQQRGRKANTRPLKRRRKRGPRIPKDENINFKQSELPVTCGEVKGTLYKERFKQGTSKKCI QSEDKKWFTPREFEIEGDRGASKNWKLSIRCGGYTLKVLMENKFLPEPPSTRKKRILESHNNTLVDPCEE HKKKNPDASVKFSEFLKKCSETWKTIFAKEKGKFEDMAKADKAHYEREMKTYIPPKGEKKKKFKDPNAPK RPPLAFFLFCSEYRPKIKGEHPGLSIDDVVKKLAGMWNNTAAADKQFYEKKAAKLKEKYKKDIAAYRAKG KPNSAKKRVVKAEKSKKKKEEEEDEEDEQEEENEEDDDK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_003113
ORF Size	2637 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_003113.4
RefSeq Size	3688 bp
RefSeq ORF	2640 bp
Locus ID	6672
UniProt ID	P23497
Domains	SAND, HMG, Sp100
Protein Families	Transcription Factors
MW	100.4 kDa
Gene Summary	This gene encodes a subnuclear organelle and major component of the PML (promyelocytic leukemia)-SP100 nuclear bodies. PML and SP100 are covalently modified by the SUMO-1 modifier, which is considered crucial to nuclear body interactions. The encoded protein binds heterochromatin proteins and is thought to play a role in tumorigenesis, immunity, and gene regulation. Alternatively spliced variants have been identified for this gene; one of which encodes a high-mobility group protein. [provided by RefSeq, Aug 2011]

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