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TIAL1 (NM_003252) Human Tagged ORF Clone
CAT＃: RC223971
TIAL1 (Myc-DDK-tagged)-Human TIA1 cytotoxic granule-associated RNA binding protein-like 1 (TIAL1), transcript variant 1
ORF Plasmid: tGFP
"NM_003252" in other vectors (6)
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CNY 3,656.00
CNY 4,370.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | TCBP; TIAR |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC223971 representing NM_003252
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGAAGACGACGGGCAGCCCCGGACTCTATACGTAGGTAACCTTTCCAGAGATGTGACAGAAGTCC TTATACTTCAGTTGTTCAGTCAGATTGGACCCTGTAAAAGCTGTAAAATGATAACAGAGCATACAAGCAA TGACCCATATTGCTTTGTGGAATTTTATGAACACAGAGATGCAGCTGCTGCATTAGCTGCTATGAATGGG AGAAAAATTTTGGGAAAGGAGGTCAAAGTAAACTGGGCAACCACACCAAGTAGCCAGAAAAAAGATACTT CCAATCACTTCCATGTGTTTGTTGGGGATTTGAGTCCAGAAATTACAACAGAAGATATCAAATCAGCATT TGCCCCCTTTGGTAAAATATCGGATGCCCGGGTAGTTAAAGACATGGCAACTGGAAAATCCAAAGGCTAT GGTTTTGTATCTTTTTATAACAAACTGGATGCAGAAAATGCGATTGTGCATATGGGCGGTCAGTGGTTGG GTGGTCGTCAAATCCGAACCAATTGGGCCACTCGTAAACCACCTGCACCTAAAAGTACACAAGAAAACAA CACTAAGCAGTTGAGATTTGAAGATGTAGTAAACCAGTCAAGTCCAAAAAATTGTACTGTGTACTGTGGA GGAATTGCGTCTGGGTTAACAGATCAGCTTATGAGACAGACATTCTCACCATTTGGACAAATTATGGAAA TAAGAGTTTTCCCAGAAAAGGGCTATTCATTTGTCAGATTTTCAACCCATGAAAGTGCAGCCCATGCCAT TGTTTCGGTGAACGGTACTACGATTGAAGGACATGTGGTTAAATGCTATTGGGGTAAAGAATCTCCTGAT ATGACTAAAAACTTCCAACAGGTTGACTATAGTCAATGGGGCCAATGGAGCCAAGTGTATGGAAACCCAC AACAGTATGGACAGTATATGGCAAATGGGTGGCAAGTACCGCCTTATGGAGTATACGGGCAACCATGGAA TCAACAAGGATTTGGAGTAGATCAATCACCTTCTGCTGCTTGGATGGGTGGATTTGGTGCTCAGCCTCCC CAAGGACAAGCTCCTCCCCCTGTAATACCTCCTCCTAACCAAGCCGGATATGGTATGGCAAGTTACCAAA CACAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC223971 representing NM_003252
Red=Cloning site Green=Tags(s) MMEDDGQPRTLYVGNLSRDVTEVLILQLFSQIGPCKSCKMITEHTSNDPYCFVEFYEHRDAAAALAAMNG RKILGKEVKVNWATTPSSQKKDTSNHFHVFVGDLSPEITTEDIKSAFAPFGKISDARVVKDMATGKSKGY GFVSFYNKLDAENAIVHMGGQWLGGRQIRTNWATRKPPAPKSTQENNTKQLRFEDVVNQSSPKNCTVYCG GIASGLTDQLMRQTFSPFGQIMEIRVFPEKGYSFVRFSTHESAAHAIVSVNGTTIEGHVVKCYWGKESPD MTKNFQQVDYSQWGQWSQVYGNPQQYGQYMANGWQVPPYGVYGQPWNQQGFGVDQSPSAAWMGGFGAQPP QGQAPPPVIPPPNQAGYGMASYQTQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003252 |
| ORF Size | 1125 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003252.3 |
| RefSeq Size | 1436 bp |
| RefSeq ORF | 1128 bp |
| Locus ID | 7073 |
| UniProt ID | Q01085 |
| Domains | RRM, RRM_1 |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 41.4 kDa |
| Gene Summary | The protein encoded by this gene is a member of a family of RNA-binding proteins, has three RNA recognition motifs (RRMs), and binds adenine and uridine-rich elements in mRNA and pre-mRNAs of a wide range of genes. It regulates various activities including translational control, splicing and apoptosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The different isoforms have been show to function differently with respect to post-transcriptional silencing. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223971L1 | Lenti ORF clone of Human TIA1 cytotoxic granule-associated RNA binding protein-like 1 (TIAL1), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC223971L2 | Lenti ORF clone of Human TIA1 cytotoxic granule-associated RNA binding protein-like 1 (TIAL1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC223971L3 | Lenti ORF clone of Human TIA1 cytotoxic granule-associated RNA binding protein-like 1 (TIAL1), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC223971L4 | Lenti ORF clone of Human TIA1 cytotoxic granule-associated RNA binding protein-like 1 (TIAL1), transcript variant 1, mGFP tagged |
CNY 6,056.00 |
|
| RG223971 | TIAL1 (tGFP-tagged) - Human TIA1 cytotoxic granule-associated RNA binding protein-like 1 (TIAL1), transcript variant 1 |
CNY 5,256.00 |
|
| SC109837 | TIAL1 (untagged)-Human TIA1 cytotoxic granule-associated RNA binding protein-like 1 (TIAL1), transcript variant 1 |
CNY 3,656.00 |
