NNT (NM_012343) Human Tagged ORF Clone
CAT#: RC224002
NNT (Myc-DDK-tagged)-Human nicotinamide nucleotide transhydrogenase (NNT), nuclear gene encoding mitochondrial protein, transcript variant 1
ORF Plasmid: tGFP
"NM_012343" in other vectors (6)
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CNY 7,560.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | GCCD4 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC224002 representing NM_012343
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAACCTATTGAAAACAGTGGTGACTGGCTGCTCGTGTCCTCTACTTAGCAATTTGGGGTCCTGTA AGGGTCTACGTGTGAAGAAGGATTTTTTACGAACATTTTATACTCACCAAGAACTGTGGTGTAAAGCGCC TGTAAAACCAGGAATTCCATATAAGCAACTGACTGTTGGAGTCCCCAAAGAGATATTCCAAAATGAGAAG CGAGTGGCATTGTCTCCTGCTGGTGTTCAGAACTTGGTCAAGCAGGGTTTTAATGTTGTCGTGGAATCGG GTGCGGGCGAAGCTTCCAAGTTCTCAGATGATCACTATAGAGTGGCAGGTGCCCAAATCCAAGGGGCAAA GGAAGTGCTGGCTTCTGATTTGGTGGTCAAAGTGCGAGCCCCTATGGTTAATCCAACATTAGGTGTTCAT GAAGCTGACCTTTTAAAGACATCAGGAACGCTGATTAGTTTTATTTACCCAGCCCAAAATCCAGAGTTGC TAAATAAACTTTCCCAAAGAAAAACTACAGTTCTGGCAATGGACCAGGTTCCAAGAGTCACAATTGCTCA GGGATATGATGCGCTAAGCTCCATGGCCAACATTGCGGGTTATAAGGCTGTTGTCCTAGCAGCAAATCAT TTTGGACGTTTTTTTACTGGTCAGATCACAGCTGCTGGAAAAGTTCCTCCAGCTAAGATTCTGATAGTTG GTGGTGGTGTTGCTGGGCTTGCTTCTGCAGGCGCAGCAAAGTCGATGGGTGCAATTGTTCGAGGATTTGA CACAAGAGCTGCAGCTTTGGAACAGTTCAAGTCTCTTGGTGCTGAGCCCTTGGAGGTGGACTTGAAGGAA TCTGGTGAGGGACAAGGAGGATATGCAAAAGAGATGTCCAAAGAGTTCATTGAAGCTGAAATGAAACTCT TTGCTCAACAATGCAAGGAGGTAGACATCCTTATCAGCACAGCACTTATTCCAGGTAAAAAAGCTCCAGT TTTATTTAATAAAGAAATGATTGAGTCAATGAAGGAAGGTTCAGTTGTTGTGGATTTAGCTGCTGAGGCT GGTGGAAACTTTGAAACCACTAAGCCAGGAGAACTCTACATTCATAAGGGAATTACTCACATAGGCTACA CAGACCTGCCCAGCCGAATGGCCACTCAGGCCAGCACCCTATATTCCAACAACATCACCAAACTCCTGAA GGCCATCAGCCCGGACAAAGATAATTTTTATTTTGATGTGAAAGATGACTTTGACTTTGGTACGATGGGT CATGTCATTAGAGGAACTGTAGTGATGAAAGATGGTAAAGTGAATTTCCCAGCTCCCACACCGAAAAATA TTCCTCAAGGTGCCCCAGTAAAACAGAAGACAGTGGCTGAGCTGGAAGCTGAAAAAGCAGCTACCATTAC ACCCTTCAGGAAGACAATGTCAACGGCTTCTGCATATACAGCAGGTCTCACAGGGATACTGGGTTTGGGC ATTGCGGCTCCCAATCTAGCCTTTTCTCAGATGGTGACCACTTTTGGCTTGGCTGGCATTGTGGGGTATC ATACCGTCTGGGGAGTGACCCCTGCTCTCCACTCACCACTGATGTCTGTGACAAATGCAATCTCAGGGCT GACTGCAGTTGGTGGGTTGGCACTGATGGGAGGACATTTGTATCCTTCCACAACTTCTCAGGGCCTTGCT GCTCTTGCTGCATTCATATCCTCTGTCAACATTGCAGGTGGCTTTCTGGTGACTCAGAGAATGCTGGACA TGTTCAAGCGTCCCACTGACCCCCCAGAATACAACTACCTGTACCTGCTCCCTGCCGGCACCTTTGTTGG TGGATATTTAGCTGCCCTCTACAGTGGTTATAACATTGAACAGATCATGTACCTAGGCTCGGGTTTGTGC TGTGTCGGTGCCTTGGCTGGCCTCTCCACCCAGGGAACAGCACGTCTTGGCAATGCACTGGGCATGATTG GGGTTGCTGGAGGACTGGCAGCCACCCTCGGAGTCCTAAAACCGGGCCCAGAATTACTAGCTCAGATGTC TGGAGCGATGGCTTTGGGTGGTACCATTGGATTGACAATTGCCAAACGCATCCAGATTTCTGATTTACCT CAATTAGTTGCTGCTTTTCACAGTTTAGTGGGTTTGGCAGCTGTACTTACTTGCATAGCTGAGTACATTA TAGAATATCCACATTTTGCTACGGATGCAGCAGCAAATCTCACCAAGATTGTGGCCTACCTCGGCACTTA CATTGGTGGCGTCACCTTTAGTGGGTCTCTCATTGCCTATGGAAAATTGCAGGGTCTCCTGAAATCTGCC CCTCTCCTACTGCCTGGAAGGCACTTACTCAATGCAGGCTTACTGGCTGCTAGTGTGGGCGGGATAATCC CATTCATGGTGGACCCAAGCTTTACTACTGGCATCACCTGTCTGGGTTCAGTGTCTGCTCTCTCTGCTGT CATGGGTGTGACTTTGACAGCTGCTATTGGGGGTGCTGACATGCCCGTCGTTATCACTGTGCTGAACAGC TACTCAGGCTGGGCCCTGTGTGCAGAGGGCTTCCTGCTCAACAACAATCTGCTGACCATCGTGGGTGCAC TCATAGGCTCGTCTGGTGCTATCCTGTCATACATCATGTGTGTGGCAATGAATCGCTCCCTGGCTAATGT GATTCTTGGAGGCTATGGCACCACTTCAACAGCTGGTGGAAAACCCATGGAAATTTCTGGCACACATACG GAAATCAACCTTGACAATGCAATTGACATGATTCGAGAAGCTAATAGCATTATTATTACACCAGGCTATG GTCTCTGTGCAGCCAAAGCTCAATACCCCATTGCTGATTTGGTAAAGATGCTCACTGAGCAAGGCAAAAA AGTCAGGTTTGGAATTCACCCAGTTGCAGGCCGAATGCCTGGTCAGCTTAATGTGCTGCTGGCTGAGGCT GGTGTGCCATATGACATTGTGTTGGAAATGGATGAGATCAACCATGATTTTCCAGATACTGATTTGGTCC TTGTAATTGGAGCTAATGACACTGTTAATTCAGCAGCTCAAGAAGATCCCAACTCTATTATTGCAGGCAT GCCAGTCCTTGAGGTCTGGAAATCAAAGCAGGTGATTGTTATGAAGAGGTCTTTGGGTGTTGGCTATGCT GCAGTGGACAATCCAATCTTCTACAAACCTAACACGGCCATGCTTCTAGGTGATGCCAAGAAAACATGTG ACGCGCTCCAGGCGAAAGTTAGAGAATCCTATCAGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224002 representing NM_012343
Red=Cloning site Green=Tags(s) MANLLKTVVTGCSCPLLSNLGSCKGLRVKKDFLRTFYTHQELWCKAPVKPGIPYKQLTVGVPKEIFQNEK RVALSPAGVQNLVKQGFNVVVESGAGEASKFSDDHYRVAGAQIQGAKEVLASDLVVKVRAPMVNPTLGVH EADLLKTSGTLISFIYPAQNPELLNKLSQRKTTVLAMDQVPRVTIAQGYDALSSMANIAGYKAVVLAANH FGRFFTGQITAAGKVPPAKILIVGGGVAGLASAGAAKSMGAIVRGFDTRAAALEQFKSLGAEPLEVDLKE SGEGQGGYAKEMSKEFIEAEMKLFAQQCKEVDILISTALIPGKKAPVLFNKEMIESMKEGSVVVDLAAEA GGNFETTKPGELYIHKGITHIGYTDLPSRMATQASTLYSNNITKLLKAISPDKDNFYFDVKDDFDFGTMG HVIRGTVVMKDGKVNFPAPTPKNIPQGAPVKQKTVAELEAEKAATITPFRKTMSTASAYTAGLTGILGLG IAAPNLAFSQMVTTFGLAGIVGYHTVWGVTPALHSPLMSVTNAISGLTAVGGLALMGGHLYPSTTSQGLA ALAAFISSVNIAGGFLVTQRMLDMFKRPTDPPEYNYLYLLPAGTFVGGYLAALYSGYNIEQIMYLGSGLC CVGALAGLSTQGTARLGNALGMIGVAGGLAATLGVLKPGPELLAQMSGAMALGGTIGLTIAKRIQISDLP QLVAAFHSLVGLAAVLTCIAEYIIEYPHFATDAAANLTKIVAYLGTYIGGVTFSGSLIAYGKLQGLLKSA PLLLPGRHLLNAGLLAASVGGIIPFMVDPSFTTGITCLGSVSALSAVMGVTLTAAIGGADMPVVITVLNS YSGWALCAEGFLLNNNLLTIVGALIGSSGAILSYIMCVAMNRSLANVILGGYGTTSTAGGKPMEISGTHT EINLDNAIDMIREANSIIITPGYGLCAAKAQYPIADLVKMLTEQGKKVRFGIHPVAGRMPGQLNVLLAEA GVPYDIVLEMDEINHDFPDTDLVLVIGANDTVNSAAQEDPNSIIAGMPVLEVWKSKQVIVMKRSLGVGYA AVDNPIFYKPNTAMLLGDAKKTCDALQAKVRESYQK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_012343 |
ORF Size | 3258 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_012343.4 |
RefSeq Size | 4600 bp |
RefSeq ORF | 3261 bp |
Locus ID | 23530 |
UniProt ID | Q13423 |
Domains | PNTB, AlaDh_PNT_C, AlaDh_PNT_N |
Protein Families | Transmembrane |
Protein Pathways | Metabolic pathways, Nicotinate and nicotinamide metabolism |
MW | 113.7 kDa |
Gene Summary | This gene encodes an integral protein of the inner mitochondrial membrane. The enzyme couples hydride transfer between NAD(H) and NADP(+) to proton translocation across the inner mitochondrial membrane. Under most physiological conditions, the enzyme uses energy from the mitochondrial proton gradient to produce high concentrations of NADPH. The resulting NADPH is used for biosynthesis and in free radical detoxification. [provided by RefSeq, Sep 2016] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Cofactor Balance by Nicotinamide Nucleotide Transhydrogenase (NNT) Coordinates Reductive Carboxylation and Glucose Catabolism in the Tricarboxylic Acid (TCA) Cycle
,Paulo A. Gameiro, Laura A. Laviolette, Joanne K. Kelleher, Othon Iliopoulos, and Gregory Stephanopoulos,
J. Biol. Chem., May 2013; 288: 12967 - 12977.
,PubMed ID 23504317
[NNT]
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC224002L1 | Lenti ORF clone of Human nicotinamide nucleotide transhydrogenase (NNT), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
CNY 9,960.00 |
|
RC224002L2 | Lenti ORF clone of Human nicotinamide nucleotide transhydrogenase (NNT), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
CNY 10,260.00 |
|
RC224002L3 | Lenti ORF clone of Human nicotinamide nucleotide transhydrogenase (NNT), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
CNY 10,260.00 |
|
RC224002L4 | Lenti ORF clone of Human nicotinamide nucleotide transhydrogenase (NNT), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
CNY 10,260.00 |
|
RG224002 | NNT (tGFP-tagged) - Human nicotinamide nucleotide transhydrogenase (NNT), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 9,160.00 |
|
SC108450 | NNT (untagged)-Human nicotinamide nucleotide transhydrogenase (NNT), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 7,568.00 |