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GNAT3 (NM_001102386) Human Tagged ORF Clone
CAT＃: RC224201
GNAT3 (Myc-DDK-tagged)-Human guanine nucleotide binding protein, alpha transducing 3 (GNAT3)
ORF Plasmid: tGFP
"NM_001102386" in other vectors (6)
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CNY 5,488.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GDCA |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC224201 representing NM_001102386
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAAGTGGAATTAGTTCAGAGAGCAAGGAGTCAGCCAAAAGATCAAAAGAACTGGAGAAAAAGCTTC AGGAGGATGCTGAGCGAGATGCAAGAACCGTAAAGCTGCTACTATTAGGAGCAGGAGAATCTGGGAAAAG TACTATTGTTAAACAAATGAAGATCATCCATAAGAATGGTTACAGTGAGCAAGAATGCATGGAGTTCAAA GCAGTAATTTACAGTAATACATTGCAATCCATCCTAGCTATTGTGAAAGCCATGACTACCCTTGGAATTG ATTATGTAAATCCCAGAAGTGCAGAGGACCAACGACAACTTTATGCAATGGCAAATACCCTGGAAGATGG TGGCATGACACCTCAACTGGCTGAGGTAATAAAACGGCTGTGGAGAGATCCAGGAATTCAGGCCTGCTTT GAAAGGGCATCTGAATATCAGCTCAATGACTCAGCAGCTTACTACCTTAATGATTTAGATAGAATAACAG CATCTGGGTATGTGCCAAATGAACAAGATGTTCTCCATTCTCGAGTGAAAACGACTGGAATCATTGAAAC TCAATTCTCCTTTAAAGACTTGCACTTCAGGATGTTTGATGTAGGTGGACAGAGATCTGAGAGAAAGAAG TGGATTCACTGCTTTGAAGGAGTTACATGCATTATATTTTGTGCTGCACTTAGTGCCTATGACATGGTCC TCGTGGAAGACGAAGAAGTGAATAGAATGCATGAAAGCCTTCACCTGTTCAACAGTATCTGTAATCACAA GTATTTTTCAACAACCTCCATTGTCCTGTTCCTCAACAAAAAAGATATCTTTCAAGAAAAGGTAACCAAG GTGCATCTTAGTATCTGCTTTCCAGAATACACTGGGCCAAATACATTTGAAGATGCAGGAAACTACATCA AGAACCAGTTTCTAGACCTGAATTTAAAAAAAGAAGATAAGGAAATTTATTCCCACATGACCTGTGCTAC TGACACCCAAAATGTCAAGTTTGTGTTTGACGCAGTTACAGATATAATAATCAAAGAGAATCTAAAAGAC TGTGGGCTTTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224201 representing NM_001102386
Red=Cloning site Green=Tags(s) MGSGISSESKESAKRSKELEKKLQEDAERDARTVKLLLLGAGESGKSTIVKQMKIIHKNGYSEQECMEFK AVIYSNTLQSILAIVKAMTTLGIDYVNPRSAEDQRQLYAMANTLEDGGMTPQLAEVIKRLWRDPGIQACF ERASEYQLNDSAAYYLNDLDRITASGYVPNEQDVLHSRVKTTGIIETQFSFKDLHFRMFDVGGQRSERKK WIHCFEGVTCIIFCAALSAYDMVLVEDEEVNRMHESLHLFNSICNHKYFSTTSIVLFLNKKDIFQEKVTK VHLSICFPEYTGPNTFEDAGNYIKNQFLDLNLKKEDKEIYSHMTCATDTQNVKFVFDAVTDIIIKENLKD CGLF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001102386 |
| ORF Size | 1062 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001102386.3 |
| RefSeq Size | 1065 bp |
| RefSeq ORF | 1065 bp |
| Locus ID | 346562 |
| UniProt ID | A8MTJ3 |
| Protein Pathways | Taste transduction |
| MW | 40.2 kDa |
| Gene Summary | Sweet, bitter, and umami tastes are transmitted from taste receptors by a specific guanine nucleotide binding protein. The protein encoded by this gene is the alpha subunit of this heterotrimeric G protein, which is found not only in the oral epithelium but also in gut tissues. Variations in this gene have been linked to metabolic syndrome. [provided by RefSeq, Dec 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224201L1 | Lenti ORF clone of Human guanine nucleotide binding protein, alpha transducing 3 (GNAT3), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC224201L2 | Lenti ORF clone of Human guanine nucleotide binding protein, alpha transducing 3 (GNAT3), mGFP tagged |
CNY 5,890.00 |
|
| RC224201L3 | Lenti ORF clone of Human guanine nucleotide binding protein, alpha transducing 3 (GNAT3), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC224201L4 | Lenti ORF clone of Human guanine nucleotide binding protein, alpha transducing 3 (GNAT3), mGFP tagged |
CNY 5,890.00 |
|
| RG224201 | GNAT3 (tGFP-tagged) - Human guanine nucleotide binding protein, alpha transducing 3 (GNAT3) |
CNY 7,088.00 |
|
| SC316926 | GNAT3 (untagged)-Human guanine nucleotide binding protein, alpha transducing 3 (GNAT3) |
CNY 5,488.00 |
