RSPO4 (NM_001029871) Human Tagged ORF Clone
CAT#: RC224295
RSPO4 (Myc-DDK-tagged)-Human R-spondin family, member 4 (RSPO4), transcript variant 1
ORF Plasmid: tGFP
"NM_001029871" in other vectors (6)
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CNY 2,400.00
CNY 3,990.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | C20orf182; CRISTIN4 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC224295 representing NM_001029871
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGGCGCCACTCTGCCTGCTCCTGCTCGTCGCCCACGCCGTGGACATGCTCGCCCTGAACCGAAGGA AGAAGCAAGTGGGCACTGGCCTGGGGGGCAACTGCACAGGCTGTATCATCTGCTCAGAGGAGAACGGCTG TTCCACCTGCCAGCAGAGGCTCTTCCTGTTCATCCGCCGGGAAGGCATCCGCCAGTACGGCAAGTGCCTG CACGACTGTCCCCCTGGGTACTTCGGCATCCGCGGCCAGGAGGTCAACAGGTGCAAAAAATGTGGGGCCA CTTGTGAGAGCTGCTTCAGCCAGGACTTCTGCATCCGGTGCAAGAGGCAGTTTTACTTGTACAAGGGGAA GTGTCTGCCCACCTGCCCGCCGGGCACTTTGGCCCACCAGAACACACGGGAGTGCCAGGGGGAGTGTGAA CTGGGTCCCTGGGGCGGCTGGAGCCCCTGCACACACAATGGAAAGACCTGCGGCTCGGCTTGGGGCCTGG AGAGCCGGGTACGAGAGGCTGGCCGGGCTGGGCATGAGGAGGCAGCCACCTGCCAGGTGCTTTCTGAGTC AAGGAAATGTCCCATCCAGAGGCCCTGCCCAGGAGAGAGGAGCCCCGGCCAGAAGAAGGGCAGGAAGGAC CGGCGCCCACGCAAGGACAGGAAGCTGGACCGCAGGCTGGACGTGAGGCCGCGCCAGCCCGGCCTGCAGC CC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224295 representing NM_001029871
Red=Cloning site Green=Tags(s) MRAPLCLLLLVAHAVDMLALNRRKKQVGTGLGGNCTGCIICSEENGCSTCQQRLFLFIRREGIRQYGKCL HDCPPGYFGIRGQEVNRCKKCGATCESCFSQDFCIRCKRQFYLYKGKCLPTCPPGTLAHQNTRECQGECE LGPWGGWSPCTHNGKTCGSAWGLESRVREAGRAGHEEAATCQVLSESRKCPIQRPCPGERSPGQKKGRKD RRPRKDRKLDRRLDVRPRQPGLQP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001029871 |
ORF Size | 702 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001029871.4 |
RefSeq Size | 2722 bp |
RefSeq ORF | 705 bp |
Locus ID | 343637 |
UniProt ID | Q2I0M5 |
Protein Families | Secreted Protein |
MW | 26.17 kDa |
Gene Summary | This gene encodes a member of the R-spondin family of proteins that share a common domain organization consisting of a signal peptide, cysteine-rich/furin-like domain, thrombospondin domain and a C-terminal basic region. The encoded protein may be involved in activation of Wnt/beta-catenin signaling pathways. Mutations in this gene are associated with anonychia congenital. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2009] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Clinical value of R-spondins in triple-negative and metaplastic breast cancers
,Coussy, F;Lallemand, F;Vacher, S;Schnitzler, A;Chemlali, W;Caly, M;Nicolas, A;Richon, S;Meseure, D;El Botty, R;De-Plater, L;Fuhrmann, L;Dubois, T;Roman-Roman, S;Dangles-Marie, V;Marangoni, E;Bièche, I;,
Br. J. Cancer
,PubMed ID 28472820
[RSPO4]
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Documents
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC224295L1 | Lenti ORF clone of Human R-spondin family, member 4 (RSPO4), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
RC224295L2 | Lenti ORF clone of Human R-spondin family, member 4 (RSPO4), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RC224295L3 | Lenti ORF clone of Human R-spondin family, member 4 (RSPO4), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
RC224295L4 | Lenti ORF clone of Human R-spondin family, member 4 (RSPO4), transcript variant 1, mGFP tagged |
CNY 4,800.00 |
|
RG224295 | RSPO4 (tGFP-tagged) - Human R-spondin family, member 4 (RSPO4), transcript variant 1 |
CNY 4,000.00 |
|
SC302453 | RSPO4 (untagged)-Human R-spondin family, member 4 (RSPO4), transcript variant 1 |
CNY 2,400.00 |