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CENPE (NM_001813) Human Tagged ORF Clone
CAT＃: RC224375
- TrueORF®
CENPE (Myc-DDK-tagged)-Human centromere protein E, 312kDa (CENPE)
ORF Plasmid: tGFP
"NM_001813" in other vectors (2)
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CNY 17,504.00
货期*
现货
规格
Product images
经常一起买 (5)
DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CENP-E; KIF10; MCPH13; PPP1R61 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC224375 representing NM_001813
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGGAAGGAGCCGTGGCCGTCTGCGTGCGAGTGCGGCCGCTGAACAGCAGAGAAGAATCACTTG GAGAAACTGCCCAAGTTTACTGGAAAACTGACAATAATGTCATTTATCAAGTTGATGGAAGTAAATCCTT CAATTTTGATCGTGTCTTTCATGGTAATGAAACTACCAAAAATGTGTATGAAGAAATAGCAGCACCAATC ATCGATTCTGCCATACAAGGCTACAATGGTACTATATTTGCCTATGGACAGACTGCTTCAGGAAAAACAT ATACCATGATGGGTTCAGAAGATCATTTGGGAGTTATACCCAGGGCAATTCATGACATTTTCCAAAAAAT TAAGAAGTTTCCTGATAGGGAATTTCTCTTACGTGTATCTTACATGGAAATATACAATGAAACCATTACA GATTTACTCTGTGGCACTCAAAAAATGAAACCTTTAATTATTCGAGAAGATGTCAATAGGAATGTGTATG TTGCTGATCTCACAGAAGAAGTTGTATATACATCAGAAATGGCTTTGAAATGGATTACAAAGGGAGAAAA GAGCAGGCATTATGGAGAAACAAAAATGAATCAAAGAAGCAGTCGTTCTCATACCATCTTTAGGATGATT TTGGAAAGCAGAGAGAAGGGTGAACCTTCTAATTGTGAAGGATCTGTTAAGGTATCCCATTTGAATTTGG TTGATCTTGCAGGCAGTGAAAGAGCTGCTCAAACAGGCGCTGCAGGTGTGCGGCTCAAGGAAGGCTGTAA TATAAATCGAAGCTTATTTATTTTGGGACAAGTGATCAAGAAACTTAGTGATGGACAAGTTGGTGGTTTC ATAAATTATCGAGATAGCAAGTTAACACGAATTCTCCAGAATTCCTTGGGAGGAAATGCAAAGACACGTA TTATCTGCACAATTACTCCAGTATCTTTTGATGAAACACTTACTGCTCTCCAGTTTGCCAGTACTGCTAA ATATATGAAGAATACTCCTTATGTTAATGAGGTATCAACTGATGAAGCTCTCCTGAAAAGGTATAGAAAA GAAATAATGGATCTTAAAAAACAATTAGAGGAGGTTTCTTTAGAGACGCGGGCTCAGGCAATGGAAAAAG ACCAATTGGCCCAACTTTTGGAAGAAAAAGATTTGCTTCAGAAAGTACAGAATGAGAAAATTGAAAACTT AACACGGATGCTGGTGACCTCTTCTTCCCTCACGTTGCAACAGGAATTAAAGGCTAAAAGAAAACGAAGA GTTACTTGGTGCCTTGGCAAAATTAACAAAATGAAGAACTCAAACTATGCAGATCAATTTAATATACCAA CAAATATAACAACAAAAACACATAAGCTTTCTATAAATTTATTACGAGAAATTGATGAATCTGTCTGTTC AGAGTCTGATGTTTTCAGTAACACTCTTGATACATTAAGTGAGATAGAATGGAATCCAGCAACAAAGCTA CTAAATCAGGAGAATATAGAAAGTGAGTTGAACTCACTTCGTGCTGACTATGATAATCTGGTATTAGACT ATGAACAACTACGAACAGAAAAAGAAGAAATGGAATTGAAATTAAAAGAAAAGAATGATTTGGATGAATT TGAGGCTCTAGAAAGAAAAACTAAAAAAGATCAAGAGATGCAACTAATTCATGAAATTTCGAACTTAAAG AATTTAGTTAAGCATGCAGAAGTATATAATCAAGATCTTGAGAATGAACTCAGTTCAAAAGTAGAGCTGC TTAGAGAAAAGGAAGACCAGATTAAGAAGCTACAGGAATACATAGACTCTCAAAAGCTAGAAAATATAAA AATGGACTTGTCATACTCATTGGAAAGCATTGAAGACCCAAAACAAATGAAGCAGACTCTGTTTGATGCT GAAACTGTAGCCCTTGATGCCAAGAGAGAATCAGCCTTTCTTAGAAGTGAAAATCTGGAGCTGAAGGAGA AAATGAAAGAACTTGCAACTACATACAAGCAAATGGAAAATGATATTCAGTTATATCAAAGCCAGTTGGA GGCAAAAAAGAAAATGCAAGTTGATCTGGAGAAAGAATTACAATCTGCTTTTAATGAGATAACAAAACTC ACCTCCCTTATAGATGGCAAAGTTCCAAAAGATTTGCTCTGTAATTTGGAATTGGAAGGAAAGATTACTG ATCTTCAGAAAGAACTAAATAAAGAAGTTGAAGAAAATGAAGCTTTGCGGGAAGAAGTCATTTTGCTTTC AGAATTGAAATCTTTACCTTCTGAAGTAGAAAGGCTGAGGAAAGAGATACAAGACAAATCTGAAGAGCTC CATATAATAACATCAGAAAAAGATAAATTGTTTTCTGAAGTAGTTCATAAGGAGAGTAGAGTTCAAGGTT TACTTGAAGAAATTGGGAAAACAAAAGATGACCTAGCAACTACACAGTCGAATTATAAAAGCACTGATCA AGAATTCCAAAATTTCAAAACCCTTCATATGGACTTTGAGCAAAAGTATAAGATGGTCCTTGAGGAGAAT GAGAGAATGAATCAGGAAATAGTTAATCTCTCTAAAGAAGCCCAAAAATTTGATTCGAGTTTGGGTGCTT TGAAGACCGAGCTTTCTTACAAGACCCAAGAACTTCAGGAGAAAACACGTGAGGTTCAAGAAAGACTAAA TGAGATGGAACAGCTGAAGGAACAATTAGAAAATAGAGATTCTACGCTGCAAACTGTAGAAAGGGAGAAA ACACTGATTACTGAGAAACTGCAGCAAACTTTAGAAGAAGTAAAAACTTTAACTCAAGAAAAAGATGATC TAAAACAACTCCAAGAAAGCTTGCAAATTGAGAGGGACCAACTCAAAAGTGATATTCACGATACTGTTAA CATGAATATAGATACTCAAGAACAATTACGAAATGCTCTTGAGTCTCTGAAACAACATCAAGAAACAATT AATACACTAAAATCGAAAATTTCTGAGGAAGTTTCCAGGAATTTGCATATGGAGGAAAATACAGGAGAAA CTAAAGATGAATTTCAGCAAAAGATGGTTGGCATAGATAAAAAACAGGATTTGGAAGCTAAAAATACCCA AACACTAACTGCAGATGTTAAGGATAATGAGATAATTGAGCAACAAAGGAAGATATTTTCTTTAATACAG GAGAAAAATGAACTCCAACAAATGTTAGAGAGTGTTATAGCAGAAAAGGAACAATTGAAGACTGACCTAA AGGAAAATATTGAAATGACCATTGAAAACCAGGAAGAATTAAGACTTCTTGGGGATGAACTTAAAAAGCA ACAAGAGATAGTTGCACAAGAAAAGAACCATGCCATAAAGAAAGAAGGAGAGCTTTCTAGGACCTGTGAC AGACTGGCAGAAGTTGAAGAAAAACTAAAGGAAAAGAGCCAGCAACTCCAAGAAAAACAGCAACAACTTC TTAATGTACAAGAAGAGATGAGTGAGATGCAGAAAAAGATTAATGAAATAGAGAATTTAAAGAATGAATT AAAGAACAAAGAATTGACATTGGAACATATGGAAACAGAGAGGCTTGAGTTGGCTCAGAAACTTAATGAA AATTATGAGGAAGTGAAATCTATAACCAAAGAAAGAAAAGTTCTAAAGGAATTACAGAAGTCATTTGAAA CAGAGAGAGACCACCTTAGAGGATATATAAGAGAAATTGAAGCTACAGGCCTACAAACCAAAGAAGAACT AAAAATTGCTCATATTCACCTAAAAGAACACCAAGAAACTATTGATGAACTAAGAAGAAGCGTATCTGAG AAGACAGCTCAAATAATAAATACTCAGGACTTAGAAAAATCCCATACCAAATTACAAGAAGAGATCCCAG TGCTTCATGAGGAACAAGAGTTACTGCCTAATGTGAAAGAAGTCAGTGAGACTCAGGAAACAATGAATGA ACTGGAGTTATTAACAGAACAGTCCACAACCAAGGACTCAACAACACTGGCAAGAATAGAAATGGAAAGG CTCAGGTTGAATGAAAAATTTCAAGAAAGTCAGGAAGAGATAAAATCTCTAACCAAGGAAAGAGACAACC TTAAAACGATAAAAGAAGCCCTTGAAGTTAAACATGACCAGCTGAAAGAACATATTAGAGAAACTTTGGC TAAAATCCAGGAGTCTCAAAGCAAACAAGAACAGTCCTTAAATATGAAAGAAAAAGACAATGAAACTACC AAAATCGTGAGTGAGATGGAGCAATTCAAACCCAAAGATTCAGCACTACTAAGGATAGAAATAGAAATGC TCGGATTGTCCAAAAGACTTCAAGAAAGTCATGATGAAATGAAATCTGTAGCTAAGGAGAAAGATGACCT ACAGAGGCTGCAAGAAGTTCTTCAATCTGAAAGTGACCAGCTCAAAGAAAACATAAAAGAAATTGTAGCT AAACACCTGGAAACTGAAGAGGAACTTAAAGTTGCTCATTGTTGCCTGAAAGAACAAGAGGAAACTATTA ATGAGTTAAGAGTGAATCTTTCAGAGAAGGAAACTGAAATATCAACCATTCAAAAGCAGTTAGAAGCAAT CAATGATAAATTACAGAACAAGATCCAAGAGATTTATGAGAAAGAGGAACAATTTAATATAAAACAAATT AGTGAGGTTCAGGAAAAAGTGAATGAACTGAAACAATTCAAGGAGCATCGCAAAGCCAAGGATTCAGCAC TACAAAGTATAGAAAGTAAGATGCTCGAGTTGACCAACAGACTTCAAGAAAGTCAAGAAGAAATACAAAT TATGATTAAGGAAAAAGAGGAAATGAAAAGAGTACAGGAGGCCCTTCAGATAGAGAGAGACCAACTGAAA GAAAACACTAAAGAAATTGTAGCTAAAATGAAAGAATCTCAAGAAAAAGAATATCAGTTTCTTAAGATGA CAGCTGTCAATGAGACTCAGGAGAAAATGTGTGAAATAGAACACTTGAAGGAGCAATTTGAGACCCAGAA GTTAAACCTGGAAAACATAGAAACGGAGAATATAAGGTTGACTCAGATACTACATGAAAACCTTGAAGAA ATGAGATCTGTAACAAAAGAAAGAGATGACCTTAGGAGTGTGGAGGAGACTCTCAAAGTAGAGAGAGACC AGCTCAAGGAAAACCTTAGAGAAACTATAACTAGAGACCTAGAAAAACAAGAGGAGCTAAAAATTGTTCA CATGCATCTGAAGGAGCACCAAGAAACTATTGATAAACTAAGAGGGATTGTTTCAGAGAAAACAAATGAA ATATCAAATATGCAAAAGGACTTAGAACACTCAAATGATGCCTTAAAAGCACAGGATCTGAAAATACAAG AGGAACTAAGAATTGCTCACATGCATCTGAAAGAGCAGCAGGAAACTATTGACAAACTCAGAGGAATTGT TTCTGAGAAGACAGATAAACTATCAAATATGCAAAAAGATTTAGAAAATTCAAATGCTAAATTACAAGAA AAGATTCAAGAACTTAAGGCAAATGAACATCAACTTATTACGTTAAAAAAAGATGTCAATGAGACACAGA AAAAAGTGTCTGAAATGGAGCAACTAAAGAAACAAATAAAAGACCAAAGCTTAACTCTGAGTAAATTAGA AATAGAGAATTTAAATTTGGCTCAGAAACTTCATGAAAACCTTGAAGAAATGAAATCTGTAATGAAAGAA AGAGATAATCTAAGAAGAGTAGAGGAGACACTCAAACTGGAGAGAGACCAACTCAAGGAAAGCCTGCAAG AAACCAAAGCTAGAGATCTGGAAATACAACAGGAACTAAAAACTGCTCGTATGCTATCAAAAGAACACAA AGAAACTGTTGATAAACTTAGAGAAAAAATTTCAGAAAAGACAATTCAAATTTCAGACATTCAAAAGGAT TTAGATAAATCAAAAGATGAATTACAGAAAAAGATCCAAGAACTTCAGAAAAAAGAACTTCAACTGCTTA GAGTGAAAGAAGATGTCAATATGAGTCATAAAAAAATTAATGAAATGGAACAGTTGAAGAAGCAATTTGA GGCCCAAAACTTATCTATGCAAAGTGTGAGAATGGATAACTTCCAGTTGACTAAGAAACTTCATGAAAGC CTTGAAGAAATAAGAATTGTAGCTAAAGAAAGAGATGAGCTAAGGAGGATAAAAGAATCTCTCAAAATGG AAAGGGACCAATTCATAGCAACCTTAAGGGAAATGATAGCTAGAGACCGACAGAACCACCAAGTAAAACC TGAAAAAAGGTTACTAAGTGATGGACAACAGCACCTTACGGAAAGCCTGAGAGAAAAGTGCTCTAGAATA AAAGAGCTTTTGAAGAGATACTCAGAGATGGATGATCATTATGAGTGCTTGAATAGATTGTCTCTTGACT TGGAGAAGGAAATTGAATTCCAAAAAGAGCTTTCAATGAGAGTTAAAGCAAACCTCTCACTTCCCTATTT ACAAACCAAACACATTGAAAAACTTTTTACTGCAAACCAGAGATGCTCCATGGAATTCCACAGAATCATG AAGAAACTGAAGTATGTGTTAAGCTATGTTACAAAAATAAAAGAAGAACAACATGAATCCATCAATAAAT TTGAAATGGATTTTATTGATGAAGTGGAAAAGCAAAAGGAATTGCTAATTAAAATACAGCACCTTCAACA AGATTGTGATGTACCATCCAGAGAATTAAGGGATCTCAAATTGAACCAGAATATGGATCTACATATTGAG GAAATTCTCAAAGATTTCTCAGAAAGTGAGTTCCCTAGCATAAAGACTGAATTTCAACAAGTACTAAGTA ATAGGAAAGAAATGACACAGTTTTTGGAAGAGTGGTTAAATACTCGTTTTGATATAGAAAAGCTTAAAAA TGGCATCCAGAAAGAAAATGATAGGATTTGTCAAGTGAATAACTTCTTTAATAACAGAATAATTGCCATA ATGAATGAATCAACAGAGTTTGAGGAAAGAAGTGCTACCATATCCAAAGAGTGGGAACAGGACCTGAAAT CACTGAAAGAGAAAAATGAAAAACTATTTAAAAACTACCAAACATTGAAGACTTCCTTGGCATCTGGTGC CCAGGTTAATCCTACCACACAAGACAATAAGAATCCTCATGTTACATCAAGAGCTACACAGTTAACCACA GAGAAAATTCGAGAGCTGGAAAATTCACTGCATGAAGCTAAAGAAAGTGCTATGCATAAGGAAAGCAAGA TTATAAAGATGCAGAAAGAACTTGAGGTGACTAATGACATAATAGCAAAACTTCAAGCCAAAGTTCATGA ATCAAATAAATGCCTTGAAAAAACAAAAGAGACAATTCAAGTACTTCAGGACAAAGTTGCTTTAGGAGCT AAGCCATATAAAGAAGAAATTGAAGATCTCAAAATGAAGCTTGTGAAAATAGACCTAGAGAAAATGAAAA ATGCCAAAGAATTTGAAAAGGAAATCAGTGCTACAAAAGCCACTGTAGAATATCAAAAGGAAGTTATAAG GCTATTGAGAGAAAATCTCAGAAGAAGTCAACAGGCCCAAGATACCTCAGTGATATCAGAACATACTGAT CCTCAGCCTTCAAATAAACCCTTAACTTGTGGAGGTGGCAGCGGCATTGTACAAAACACAAAAGCTCTTA TTTTGAAAAGTGAACATATAAGGCTAGAAAAAGAAATTTCTAAGTTAAAGCAGCAAAATGAACAGCTAAT AAAACAAAAGAATGAATTGTTAAGCAATAATCAGCATCTTTCCAATGAGGTCAAAACTTGGAAGGAAAGA ACCCTTAAAAGAGAGGCTCACAAACAAGTAACTTGTGAGAATTCTCCAAAGTCTCCTAAAGTGACTGGAA CAGCTTCTAAAAAGAAACAAATTACACCCTCTCAATGCAAGGAACGGAATTTACAAGATCCTGTGCCAAA GGAATCACCAAAATCTTGTTTTTTTGATAGCCGATCAAAGTCTTTACCATCACCTCATCCAGTTCGCTAT TTTGATAACTCAAGTTTAGGCCTTTGTCCAGAGGTGCAAAATGCAGGAGCAGAGAGTGTGGATTCTCAGC CAGGTCCTTGGCACGCCTCCTCAGGCAAGGATGTGCCTGAGTGCAAAACTCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224375 representing NM_001813
Red=Cloning site Green=Tags(s) MAEEGAVAVCVRVRPLNSREESLGETAQVYWKTDNNVIYQVDGSKSFNFDRVFHGNETTKNVYEEIAAPI IDSAIQGYNGTIFAYGQTASGKTYTMMGSEDHLGVIPRAIHDIFQKIKKFPDREFLLRVSYMEIYNETIT DLLCGTQKMKPLIIREDVNRNVYVADLTEEVVYTSEMALKWITKGEKSRHYGETKMNQRSSRSHTIFRMI LESREKGEPSNCEGSVKVSHLNLVDLAGSERAAQTGAAGVRLKEGCNINRSLFILGQVIKKLSDGQVGGF INYRDSKLTRILQNSLGGNAKTRIICTITPVSFDETLTALQFASTAKYMKNTPYVNEVSTDEALLKRYRK EIMDLKKQLEEVSLETRAQAMEKDQLAQLLEEKDLLQKVQNEKIENLTRMLVTSSSLTLQQELKAKRKRR VTWCLGKINKMKNSNYADQFNIPTNITTKTHKLSINLLREIDESVCSESDVFSNTLDTLSEIEWNPATKL LNQENIESELNSLRADYDNLVLDYEQLRTEKEEMELKLKEKNDLDEFEALERKTKKDQEMQLIHEISNLK NLVKHAEVYNQDLENELSSKVELLREKEDQIKKLQEYIDSQKLENIKMDLSYSLESIEDPKQMKQTLFDA ETVALDAKRESAFLRSENLELKEKMKELATTYKQMENDIQLYQSQLEAKKKMQVDLEKELQSAFNEITKL TSLIDGKVPKDLLCNLELEGKITDLQKELNKEVEENEALREEVILLSELKSLPSEVERLRKEIQDKSEEL HIITSEKDKLFSEVVHKESRVQGLLEEIGKTKDDLATTQSNYKSTDQEFQNFKTLHMDFEQKYKMVLEEN ERMNQEIVNLSKEAQKFDSSLGALKTELSYKTQELQEKTREVQERLNEMEQLKEQLENRDSTLQTVEREK TLITEKLQQTLEEVKTLTQEKDDLKQLQESLQIERDQLKSDIHDTVNMNIDTQEQLRNALESLKQHQETI NTLKSKISEEVSRNLHMEENTGETKDEFQQKMVGIDKKQDLEAKNTQTLTADVKDNEIIEQQRKIFSLIQ EKNELQQMLESVIAEKEQLKTDLKENIEMTIENQEELRLLGDELKKQQEIVAQEKNHAIKKEGELSRTCD RLAEVEEKLKEKSQQLQEKQQQLLNVQEEMSEMQKKINEIENLKNELKNKELTLEHMETERLELAQKLNE NYEEVKSITKERKVLKELQKSFETERDHLRGYIREIEATGLQTKEELKIAHIHLKEHQETIDELRRSVSE KTAQIINTQDLEKSHTKLQEEIPVLHEEQELLPNVKEVSETQETMNELELLTEQSTTKDSTTLARIEMER LRLNEKFQESQEEIKSLTKERDNLKTIKEALEVKHDQLKEHIRETLAKIQESQSKQEQSLNMKEKDNETT KIVSEMEQFKPKDSALLRIEIEMLGLSKRLQESHDEMKSVAKEKDDLQRLQEVLQSESDQLKENIKEIVA KHLETEEELKVAHCCLKEQEETINELRVNLSEKETEISTIQKQLEAINDKLQNKIQEIYEKEEQFNIKQI SEVQEKVNELKQFKEHRKAKDSALQSIESKMLELTNRLQESQEEIQIMIKEKEEMKRVQEALQIERDQLK ENTKEIVAKMKESQEKEYQFLKMTAVNETQEKMCEIEHLKEQFETQKLNLENIETENIRLTQILHENLEE MRSVTKERDDLRSVEETLKVERDQLKENLRETITRDLEKQEELKIVHMHLKEHQETIDKLRGIVSEKTNE ISNMQKDLEHSNDALKAQDLKIQEELRIAHMHLKEQQETIDKLRGIVSEKTDKLSNMQKDLENSNAKLQE KIQELKANEHQLITLKKDVNETQKKVSEMEQLKKQIKDQSLTLSKLEIENLNLAQKLHENLEEMKSVMKE RDNLRRVEETLKLERDQLKESLQETKARDLEIQQELKTARMLSKEHKETVDKLREKISEKTIQISDIQKD LDKSKDELQKKIQELQKKELQLLRVKEDVNMSHKKINEMEQLKKQFEAQNLSMQSVRMDNFQLTKKLHES LEEIRIVAKERDELRRIKESLKMERDQFIATLREMIARDRQNHQVKPEKRLLSDGQQHLTESLREKCSRI KELLKRYSEMDDHYECLNRLSLDLEKEIEFQKELSMRVKANLSLPYLQTKHIEKLFTANQRCSMEFHRIM KKLKYVLSYVTKIKEEQHESINKFEMDFIDEVEKQKELLIKIQHLQQDCDVPSRELRDLKLNQNMDLHIE EILKDFSESEFPSIKTEFQQVLSNRKEMTQFLEEWLNTRFDIEKLKNGIQKENDRICQVNNFFNNRIIAI MNESTEFEERSATISKEWEQDLKSLKEKNEKLFKNYQTLKTSLASGAQVNPTTQDNKNPHVTSRATQLTT EKIRELENSLHEAKESAMHKESKIIKMQKELEVTNDIIAKLQAKVHESNKCLEKTKETIQVLQDKVALGA KPYKEEIEDLKMKLVKIDLEKMKNAKEFEKEISATKATVEYQKEVIRLLRENLRRSQQAQDTSVISEHTD PQPSNKPLTCGGGSGIVQNTKALILKSEHIRLEKEISKLKQQNEQLIKQKNELLSNNQHLSNEVKTWKER TLKREAHKQVTCENSPKSPKVTGTASKKKQITPSQCKERNLQDPVPKESPKSCFFDSRSKSLPSPHPVRY FDNSSLGLCPEVQNAGAESVDSQPGPWHASSGKDVPECKTQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001813 |
| ORF Size | 8103 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001813.3 |
| RefSeq Size | 8630 bp |
| RefSeq ORF | 8106 bp |
| Locus ID | 1062 |
| UniProt ID | Q02224 |
| Protein Families | Druggable Genome, Stem cell - Pluripotency |
| MW | 316.2 kDa |
| Gene Summary | Centrosome-associated protein E (CENPE) is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. This protein is required for stable spindle microtubule capture at kinetochores which is a necessary step in chromosome alignment during prometaphase. This protein also couples chromosome position to microtubule depolymerizing activity. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms. [provided by RefSeq, Nov 2014] |
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