PPIG (NM_004792) Human Tagged ORF Clone

CAT#: RC224587

PPIG (Myc-DDK-tagged)-Human peptidylprolyl isomerase G (cyclophilin G) (PPIG)

ORF Plasmid: DDK tGFP



  "NM_004792" in other vectors (4)

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CNY 5,520.00

CNY 5,800.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


PPIG rabbit polyclonal antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms CARS-Cyp; CYP; SCAF10; SRCyp
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC224587 representing NM_004792
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAATAAAGGTTCAACGTCCTCGATGTTTTTTTGACATTGCCATTAACAATCAACCTGCTGGAAGAG
TTGTCTTTGAATTATTTTCTGATGTGTGCCCCAAAACATGCGAGAACTTTCGTTGTCTTTGTACAGGTGA
AAAGGGGACCGGGAAATCAACTCAGAAACCATTACATTATAAGAGTTGTCTCTTTCACAGAGTTGTCAAG
GATTTTATGGTTCAAGGTGGTGACTTCAGTGAAGGAAATGGACGAGGAGGGGAATCTATCTATGGAGGAT
TTTTTGAAGACGAGAGTTTCGCTGTTAAACACAACAAAGAATTTCTCTTGTCAATGGCCAACAGAGGGAA
GGATACAAATGGTTCACAGTTCTTCATAACAACGAAACCAACTCCTCATTTAGATGGGCATCATGTTGTT
TTTGGACAAGTAATCTCTGGTCAAGAAGTTGTAAGAGAGATTGAAAACCAGAAAACAGATGCAGCTAGCA
AACCGTTTGCGGAGGTACGGATACTCAGTTGTGGAGAGCTGATTCCCAAATCTAAAGTTAAGAAAGAAGA
AAAGAAAAGGCATAAATCATCATCATCTTCCTCCTCCTCATCTAGTGACTCAGATAGCTCAAGTGATTCT
CAGTCCTCTTCTGATTCCTCTGATTCCGAAAGTGCTACTGAAGAGAAATCAAAGAAAAGAAAAAAGAAAC
ATCGGAAAAATTCCCGAAAACACAAGAAAGAAAAGAAAAAGCGAAAGAAAAGCAAGAAGAGTGCATCTAG
TGAGAGTGAAGCTGAAAATCTTGAAGCACAACCCCAGTCTACTGTCCGTCCAGAAGAGATCCCTCCTATA
CCTGAAAATAGATTCCTAATGAGAAAAAGTCCTCCTAAAGCTGATGAGAAGGAAAGGAAAAACAGAGAGA
GAGAAAGGGAAAGAGAGTGTAATCCACCTAACTCCCAGCCTGCTTCATACCAGAGACGACTTTTAGTTAC
TAGATCTGGCAGGAAAATTAAAGGAAGAGGACCAAGGCGTTATCGAACTCCTTCCAGATCCAGATCAAGG
GATCGTTTCAGACGTAGTGAGACTCCTCCACATTGGAGGCAAGAGATGCAGAGAGCTCAAAGAATGAGGG
TATCAAGTGGTGAAAGATGGATCAAGGGGGATAAGAGTGAGTTGAATGAAATAAAAGAAAATCAGAGAAG
TCCAGTTAGAGTAAAAGAGAGAAAAATAACAGATCACAGGAATGTATCTGAGAGTCCAAACAGAAAAAAT
GAAAAGGAGAAGAAAGTTAAAGACCATAAATCTAACAGCAAAGAGAGAGACATCAGAAGAAATTCAGAAA
AAGATGACAAGTATAAAAACAAAGTGAAGAAAAGGGCCAAATCTAAAAGTAGGAGTAAGAGCAAAGAGAA
ATCAAAGAGTAAAGAAAGAGATTCAAAACATAATAGAAATGAAGAAAAGAGGATGAGGTCAAGGAGTAAA
GGAAGGGATCATGAAAATGTTAAAGAAAAAGAAAAGCAGTCTGATTCTAAAGGAAAAGATCAGGAAAGGA
GTAGAAGTAAAGAGAAGTCTAAACAGTTAGAATCAAAGAGTAATGAGCATGATCACAGTAAAAGTAAGGA
AAAGGATAGACGCGCACAATCCAGGAGTAGAGAATGTGATATAACTAAAGGTAAACACAGTTATAATAGC
AGAACAAGAGAACGAAGCAGAAGTAGGGACAGAAGCAGAAGAGTGCGATCAAGAACCCATGACAGAGATC
GCAGCAGAAGCAAGGAGTACCATAGATACAGAGAACAGGAATACAGGAGAAGAGGACGGTCACGAAGCCG
AGAGAGAAGAACACCACCAGGAAGATCAAGAAGTAAAGATAGGAGGAGAAGGAGGAGAGACTCACGGAGC
TCAGAGAGAGAAGAAAGTCAAAGCAGAAACAAAGACAAATACAGAAACCAAGAGAGTAAGAGCTCACACA
GAAAAGAAAATTCTGAGAGTGAGAAAAGAATGTACTCTAAAAGTCGTGATCATAATAGCTCAAATAACAG
CAGGGAAAAAAAGGCTGATAGAGATCAAAGTCCCTTCTCAAAAATAAAACAAAGCAGTCAGGACAATGAA
TTAAAGTCCTCCATGTTGAAAAATAAGGAGGATGAGAAGATCAGATCCTCAGTGGAAAAAGAAAACCAAA
AATCAAAAGGTCAAGAAAATGACCATGTACATGAAAAAAATAAAAAATTTGATCATGAATCAAGCCCTGG
AACAGATGAAGACAAAAGCGGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC224587 representing NM_004792
Red=Cloning site Green=Tags(s)

MGIKVQRPRCFFDIAINNQPAGRVVFELFSDVCPKTCENFRCLCTGEKGTGKSTQKPLHYKSCLFHRVVK
DFMVQGGDFSEGNGRGGESIYGGFFEDESFAVKHNKEFLLSMANRGKDTNGSQFFITTKPTPHLDGHHVV
FGQVISGQEVVREIENQKTDAASKPFAEVRILSCGELIPKSKVKKEEKKRHKSSSSSSSSSSDSDSSSDS
QSSSDSSDSESATEEKSKKRKKKHRKNSRKHKKEKKKRKKSKKSASSESEAENLEAQPQSTVRPEEIPPI
PENRFLMRKSPPKADEKERKNRERERERECNPPNSQPASYQRRLLVTRSGRKIKGRGPRRYRTPSRSRSR
DRFRRSETPPHWRQEMQRAQRMRVSSGERWIKGDKSELNEIKENQRSPVRVKERKITDHRNVSESPNRKN
EKEKKVKDHKSNSKERDIRRNSEKDDKYKNKVKKRAKSKSRSKSKEKSKSKERDSKHNRNEEKRMRSRSK
GRDHENVKEKEKQSDSKGKDQERSRSKEKSKQLESKSNEHDHSKSKEKDRRAQSRSRECDITKGKHSYNS
RTRERSRSRDRSRRVRSRTHDRDRSRSKEYHRYREQEYRRRGRSRSRERRTPPGRSRSKDRRRRRRDSRS
SEREESQSRNKDKYRNQESKSSHRKENSESEKRMYSKSRDHNSSNNSREKKADRDQSPFSKIKQSSQDNE
LKSSMLKNKEDEKIRSSVEKENQKSKGQENDHVHEKNKKFDHESSPGTDEDKSG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_004792
ORF Size 2262 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_004792.3
RefSeq Size 2717 bp
RefSeq ORF 2265 bp
Locus ID 9360
UniProt ID Q13427
Domains pro_isomerase
MW 88.4 kDa
Gene Summary PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding (PubMed:20676357). May be implicated in the folding, transport, and assembly of proteins. May play an important role in the regulation of pre-mRNA splicing.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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