PER1 (NM_002616) Human Tagged ORF Clone
CAT#: RC224777
PER1 (Myc-DDK-tagged)-Human period homolog 1 (Drosophila) (PER1)
ORF Plasmid: tGFP
"NM_002616" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 13,472.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | hPER; PER; RIGUI |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC224777 representing NM_002616
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGGCCCCCTAGAAGGGGCTGATGGGGGAGGGGACCCCAGGCCTGGGGAATCATTTTGTCCTGGGG GCGTCCCATCCCCTGGGCCCCCACAGCACCGGCCTTGCCCAGGCCCCAGCCTGGCCGATGACACCGATGC CAACAGCAATGGTTCAAGTGGCAATGAGTCCAACGGGCATGAGTCTAGAGGCGCATCTCAGCGGAGCTCA CACAGCTCCTCCTCAGGCAACGGCAAGGACTCAGCCCTGCTGGAGACCACTGAGAGCAGCAAGAGCACAA ACTCTCAGAGCCCATCCCCACCCAGCAGTTCCATTGCCTACAGCCTCCTGAGTGCCAGCTCAGAGCAGGA CAACCCGTCCACCAGTGGCTGCAGCAGTGAACAGTCAGCCCGGGCAAGGACTCAGAAGGAACTCATGACA GCACTTCGAGAGCTCAAGCTTCGACTGCCGCCAGAGCGCCGGGGCAAGGGCCGCTCTGGGACCCTGGCCA CGCTGCAGTACGCACTGGCCTGTGTCAAGCAGGTGCAGGCCAACCAGGAATACTACCAGCAGTGGAGCCT GGAGGAGGGCGAGCCTTGCTCCATGGACATGTCCACCTATACCCTGGAGGAGCTGGAGCACATCACGTCT GAGTACACACTTCAGAACCAGGATACCTTCTCAGTGGCTGTCTCCTTCCTGACGGGCCGAATCGTCTACA TTTCGGAGCAGGCAGCCGTCCTGCTGCGTTGCAAGCGGGACGTGTTCCGGGGTACCCGCTTCTCTGAGCT CCTGGCTCCCCAGGATGTGGGAGTCTTCTATGGTTCCACTGCTCCATCTCGCCTGCCCACCTGGGGCACA GGGGCCTCAGCAGGTTCAGGCCTCAGGGACTTTACCCAGGAGAAGTCCGTCTTCTGCCGTATCAGAGGAG GTCCTGACCGGGATCCAGGGCCTCGGTACCAGCCATTCCGCCTAACCCCGTATGTGACCAAGATCCGGGT CTCAGATGGGGCCCCTGCACAGCCGTGCTGCCTGCTGATTGCAGAGCGCATCCATTCGGGTTACGAAGCT CCCCGGATACCCCCTGACAAGAGGATTTTCACTACGCGGCACACACCCAGCTGCCTCTTCCAGGATGTGG ATGAAAGGGCTGCCCCCCTGCTGGGCTACCTGCCCCAGGACCTCCTGGGGGCCCCAGTGCTCCTGTTCCT GCATCCTGAGGACCGACCCCTCATGCTGGCTATCCACAAGAAGATTCTGCAGTTGGCGGGCCAGCCCTTT GACCACTCCCCTATCCGCTTCTGTGCCCGCAACGGGGAGTATGTCACCATGGACACCAGCTGGGCTGGCT TTGTGCACCCCTGGAGCCGCAAGGTAGCCTTCGTGTTGGGCCGCCACAAAGTACGCACGGCCCCCCTGAA TGAGGACGTGTTCACTCCCCCGGCCCCCAGCCCAGCTCCCTCCCTGGACACTGATATCCAGGAGCTGTCA GAGCAGATCCACCGGCTGCTGCTGCAGCCCGTCCACAGCCCCAGCCCCACGGGACTCTGTGGAGTCGGCG CCGTGACATCCCCAGGCCCTCTCCACAGCCCTGGGTCCTCCAGTGATAGCAACGGGGGTGATGCAGAGGG GCCTGGGCCTCCTGCGCCAGTGACTTTCCAACAGATCTGTAAGGATGTGCATCTGGTGAAGCACCAGGGC CAGCAGCTTTTTATTGAGTCTCGGGCCCGGCCTCAGTCCCGGCCCCGCCTCCCTGCTACAGGCACGTTCA AGGCCAAGGCCCTTCCCTGCCAATCCCCAGACCCAGAGCTGGAGGCGGGTTCTGCTCCCGTCCAGGCCCC ACTAGCCTTGGTCCCTGAGGAGGCCGAGAGGAAAGAAGCCTCCAGCTGCTCCTACCAGCAGATCAACTGC CTGGACAGCATCCTCAGGTACCTGGAGAGCTGCAACCTCCCCAGCACCACTAAGCGTAAATGTGCCTCCT CCTCCTCCTATACCACCTCCTCAGCCTCTGACGACGACAGGCAGAGGACAGGTCCAGTCTCTGTGGGGAC CAAGAAAGATCCGCCGTCAGCAGCGCTGTCTGGGGAGGGGGCCACCCCACGGAAGGAGCCAGTGGTGGGA GGCACCCTGAGCCCGCTCGCCCTGGCCAATAAGGCGGAGAGTGTGGTGTCCGTCACCAGTCAGTGTAGCT TCAGCTCCACCATCGTCCATGTGGGAGACAAGAAGCCCCCGGAGTCGGACATCATCATGATGGAGGACCT GCCTGGTCTAGCCCCAGGCCCAGCCCCCAGCCCAGCCCCCAGCCCCACAGTAGCCCCTGACCCAGCCCCA GACGCCTACCGTCCAGTGGGGCTGACCAAGGCCGTGCTGTCCCTGCACACGCAGAAGGAAGAGCAAGCCT TCCTCAGCCGCTTCCGAGACCTGGGCAGGCTGCGTGGACTCGACAGCTCTTCCACAGCTCCCTCAGCCCT TGGCGAGCGAGGCTGCCACCACGGCCCCGCACCCCCAAGCCGCCGACACCACTGCCGATCCAAAGCCAAG CGCTCACGCCACCACCAGAACCCTCGGGCTGAAGCGCCCTGCTATGTCTCACACCCCTCACCCGTGCCAC CCTCCACCCCCTGGCCCACCCCACCAGCCACTACCCCCTTCCCAGCGGTTGTCCAGCCCTACCCTCTCCC AGTGTTCTCTCCTCGAGGAGGCCCCCAGCCTCTTCCCCCTGCTCCCACATCTGTGCCCCCAGCTGCTTTC CCCGCCCCTTTGGTGACCCCAATGGTGGCCTTGGTGCTCCCTAACTATCTGTTCCCAACCCCATCCAGCT ATCCTTATGGGGCACTCCAGACCCCTGCTGAAGGGCCTCCCACTCCTGCCTCGCACTCCCCTTCTCCATC CTTGCCCGCCCTCCCCCCGAGTCCTCCTCACCGCCCGGACTCTCCACTGTTCAACTCGAGATGCAGCTCT CCACTCCAGCTCAATCTGCTGCAGCTGGAGGAGCTCCCCCGTGCTGAGGGGGCTGCTGTTGCAGGAGGCC CTGGGAGCAGTGCCGGGCCCCCACCTCCCAGTGCGGAGGCTGCTGAGCCAGAGGCCAGACTGGCGGAGGT CACTGAGTCCTCCAATCAGGACGCACTTTCCGGCTCCAGTGACCTGCTCGAACTTCTGCTGCAAGAGGAC TCGCGCTCCGGCACAGGCTCCGCAGCCTCGGGCTCCTTGGGCTCTGGCTTGGGCTCTGGGTCTGGTTCAG GCTCCCATGAAGGGGGCAGCACCTCAGCCAGCATCACTCGCAGCAGCCAGAGCAGCCACACAAGCAAATA CTTTGGCAGCATCGACTCTTCCGAGGCTGAGGCTGGGGCTGCTCGGGGCGGGGCTGAGCCTGGGGACCAG GTGATTAAGTACGTGCTCCAGGATCCCATTTGGCTGCTCATGGCCAATGCTGACCAGCGCGTCATGATGA CCTACCAGGTGCCCTCCAGGGACATGACCTCTGTGCTGAAGCAGGATCGGGAGCGGCTCCGAGCCATGCA GAAGCAGCAGCCTCGGTTTTCTGAGGACCAGCGGCGGGAACTGGGTGCTGTGCACTCCTGGGTCCGGAAG GGCCAACTGCCTCGGGCTCTTGATGTGATGGCCTGTGTGGACTGTGGGAGCAGCACCCAAGATCCTGGTC ACCCTGATGACCCACTCTTCTCAGAGCTGGATGGACTGGGGCTGGAGCCCATGGAAGAGGGTGGAGGCGA GCAGGGCAGCAGCGGTGGCGGCAGTGGTGAGGGAGAGGGCTGCGAGGAGGCCCAAGGCGGGGCCAAGGCT TCAAGCTCTCAGGACTTGGCTATGGAGGAGGAGGAAGAAGGCAGGAGCTCATCCAGTCCAGCCTTACCTA CAGCAGGAAACTGCACCAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224777 representing NM_002616
Red=Cloning site Green=Tags(s) MSGPLEGADGGGDPRPGESFCPGGVPSPGPPQHRPCPGPSLADDTDANSNGSSGNESNGHESRGASQRSS HSSSSGNGKDSALLETTESSKSTNSQSPSPPSSSIAYSLLSASSEQDNPSTSGCSSEQSARARTQKELMT ALRELKLRLPPERRGKGRSGTLATLQYALACVKQVQANQEYYQQWSLEEGEPCSMDMSTYTLEELEHITS EYTLQNQDTFSVAVSFLTGRIVYISEQAAVLLRCKRDVFRGTRFSELLAPQDVGVFYGSTAPSRLPTWGT GASAGSGLRDFTQEKSVFCRIRGGPDRDPGPRYQPFRLTPYVTKIRVSDGAPAQPCCLLIAERIHSGYEA PRIPPDKRIFTTRHTPSCLFQDVDERAAPLLGYLPQDLLGAPVLLFLHPEDRPLMLAIHKKILQLAGQPF DHSPIRFCARNGEYVTMDTSWAGFVHPWSRKVAFVLGRHKVRTAPLNEDVFTPPAPSPAPSLDTDIQELS EQIHRLLLQPVHSPSPTGLCGVGAVTSPGPLHSPGSSSDSNGGDAEGPGPPAPVTFQQICKDVHLVKHQG QQLFIESRARPQSRPRLPATGTFKAKALPCQSPDPELEAGSAPVQAPLALVPEEAERKEASSCSYQQINC LDSILRYLESCNLPSTTKRKCASSSSYTTSSASDDDRQRTGPVSVGTKKDPPSAALSGEGATPRKEPVVG GTLSPLALANKAESVVSVTSQCSFSSTIVHVGDKKPPESDIIMMEDLPGLAPGPAPSPAPSPTVAPDPAP DAYRPVGLTKAVLSLHTQKEEQAFLSRFRDLGRLRGLDSSSTAPSALGERGCHHGPAPPSRRHHCRSKAK RSRHHQNPRAEAPCYVSHPSPVPPSTPWPTPPATTPFPAVVQPYPLPVFSPRGGPQPLPPAPTSVPPAAF PAPLVTPMVALVLPNYLFPTPSSYPYGALQTPAEGPPTPASHSPSPSLPALPPSPPHRPDSPLFNSRCSS PLQLNLLQLEELPRAEGAAVAGGPGSSAGPPPPSAEAAEPEARLAEVTESSNQDALSGSSDLLELLLQED SRSGTGSAASGSLGSGLGSGSGSGSHEGGSTSASITRSSQSSHTSKYFGSIDSSEAEAGAARGGAEPGDQ VIKYVLQDPIWLLMANADQRVMMTYQVPSRDMTSVLKQDRERLRAMQKQQPRFSEDQRRELGAVHSWVRK GQLPRALDVMACVDCGSSTQDPGHPDDPLFSELDGLGLEPMEEGGGEQGSSGGGSGEGEGCEEAQGGAKA SSSQDLAMEEEEEGRSSSSPALPTAGNCTS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_002616 |
ORF Size | 3870 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_002616.1, NP_002607.1 |
RefSeq Size | 4656 bp |
RefSeq ORF | 3873 bp |
Locus ID | 5187 |
UniProt ID | O15534 |
Protein Families | Druggable Genome |
Protein Pathways | Circadian rhythm - mammal |
MW | 136 kDa |
Gene Summary | This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene may increase the risk of getting certain cancers. Alternative splicing has been observed in this gene; however, these variants have not been fully described. [provided by RefSeq, Jan 2014] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC224777L1 | Lenti-ORF clone of PER1 (Myc-DDK-tagged)-Human period homolog 1 (Drosophila) (PER1) |
CNY 15,872.00 |
|
RC224777L2 | Lenti-ORF clone of PER1 (mGFP-tagged)-Human period homolog 1 (Drosophila) (PER1) |
CNY 15,872.00 |
|
RC224777L3 | Lenti-ORF clone of PER1 (Myc-DDK-tagged)-Human period homolog 1 (Drosophila) (PER1) |
CNY 7,600.00 |
|
RC224777L4 | Lenti-ORF clone of PER1 (mGFP-tagged)-Human period homolog 1 (Drosophila) (PER1) |
CNY 7,600.00 |
|
RG224777 | PER1 (tGFP-tagged) - Human period homolog 1 (Drosophila) (PER1) |
CNY 15,072.00 |
|
SC303227 | PER1 (untagged)-Human period homolog 1 (Drosophila) (PER1) |
CNY 13,480.00 |