United States
Germany
Japan
United Kingdom
China
CEMIP2 (NM_013390) Human Tagged ORF Clone
CAT＃: RC224793
- TrueORF®
TMEM2 (Myc-DDK-tagged)-Human transmembrane protein 2 (TMEM2), transcript variant 1
ORF Plasmid: tGFP
"NM_013390" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 9,464.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | TMEM2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC224793 representing NM_013390
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTATGCCACTGATTCCAGGGGACACTCCCCTGCTTTCCTCCAACCTCAGAATGGAAATAGTCGTCACC CATCTGGCTATGTTCCAGGGAAGGTTGTCCCATTGCGTCCCCCTCCTCCTCCAAAGAGTCAAGCTTCAGC CAAATTTACCTCCATCAGACGAGAAGACCGGGCAACCTTCGCATTCTCACCTGAAGAACAGCAAGCCCAG AGAGAAAGTCAAAAGCAAAAGAGACACAAAAATACTTTCATTTGTTTTGCTATTACTAGTTTCTCATTTT TTATTGCACTTGCAATCATTTTAGGAATATCCTCAAAATATGCTCCAGATGAAAATTGCCCAGATCAAAA TCCTCGTCTCAGGAATTGGGATCCAGGACAAGATTCTGCAAAGCAAGTTGTTATCAAGGAGGGAGATATG CTCCGTCTGACCTCAGACGCCACCGTGCATTCTATAGTCATTCAGGATGGAGGACTGCTTGTATTTGGGG ACAATAAAGATGGATCCAGAAATATTACTTTGAGGACTCATTACATCCTGATCCAGGATGGTGGGGCGCT TCATATTGGAGCAGAAAAATGCCGCTATAAATCCAAAGCGACAATTACCTTGTATGGCAAGTCAGATGAA GGTGAAAGTATGCCAACATTTGGCAAAAAGTTTATTGGTGTGGAAGCTGGCGGGACACTGGAGTTACATG GGGCACGGAAGGCATCGTGGACGTTGTTGGCAAGGACCCTGAATTCCTCAGGCTTGCCCTTTGGGTCCTA TACCTTTGAAAAGGACTTTTCCCGGGGCCTCAATGTGAGGGTCATTGACCAAGACACGGCCAAAATTTTG GAAAGTGAGAGATTTGATACCCATGAATACCGCAATGAGAGCAGGCGGCTTCAGGAGTTTCTGAGATTCC AGGATCCAGGTCGGATTGTTGCCATAGCTGTCGGGGATTCAGCCGCTAAAAGTCTCTTACAAGGAACCAT CCAGATGATCCAGGAACGGTTGGGAAGTGAACTGATCCAAGGACTGGGCTACAGGCAAGCTTGGGCTTTA GTTGGTGTCATTGATGGTGGAAGCACTTCTTGCAATGAATCCGTGAGAAACTATGAAAATCATAGCAGTG GCGGGAAGGCTCTTGCCCAAAGAGAATTTTATACTGTGGATGGCCAGAAGTTCTCTGTGACAGCTTATAG TGAATGGATTGAAGGCGTTTCTCTTTCAGGATTCCGGGTAGAGGTTGTAGATGGAGTGAAGCTAAATTTG CTAGATGATGTTAGTAGTTGGAAACCTGGAGACCAGATTGTGGTCGCAAGCACAGACTATTCCATGTACC AAGCAGAGGAGTTCACTCTTCTTCCCTGTTCTGAATGCAGCCATTTTCAGGTCAAAGTCAAAGAAACCCC TCAGTTCCTGCACATGGGTGAGATCATAGACGGTGTAGACATGAGAGCTGAGGTTGGAATTCTTACCCGG AATATTGTGATCCAAGGAGAAGTGGAGGACTCATGCTACGCAGAAAATCAGTGCCAATTTTTTGATTATG ATACCTTTGGGGGACACATTATGATAATGAAAAATTTTACTTCAGTCCATCTTTCTTATGTGGAATTGAA ACACATGGGTCAGCAGCAGATGGGGCGATACCCTGTTCATTTTCACCTGTGTGGTGACGTGGATTATAAA GGAGGATACAGACATGCAACATTTGTGGACGGCCTGTCTATTCATCACAGCTTCTCAAGGTGCATCACTG TGCATGGGACAAATGGCTTGCTAATAAAAGACACCATTGGGTTTGACACACTAGGTCATTGTTTCTTTTT GGAAGATGGTATTGAACAGAGGAATACTTTGTTCCACAATCTGGGACTCCTCACCAAGCCGGGTACTCTC CTGCCCACCGATAGGAACAACTCCATGTGTACCACCATGCGAGATAAAGTGTTTGGAAATTACATTCCTG TGCCTGCTACTGACTGTATGGCTGTTTCAACTTTCTGGATTGCTCATCCCAACAATAATCTGATTAATAA TGCAGCTGCAGGCTCACAGGATGCTGGAATATGGTATTTATTCCACAAGGAACCAACTGGGGAATCCAGT GGATTGCAGCTCTTGGCAAAACCAGAACTCACTCCATTGGGTATATTTTATAACAACAGGGTCCATTCAA ATTTTAAGGCTGGCTTATTTATTGACAAAGGTGTCAAAACAACCAACTCTAGTGCTGCTGACCCAAGGGA ATACCTCTGTTTGGACAATAGTGCAAGATTTCGACCTCATCAGGATGCAAACCCCGAAAAACCACGTGTT GCTGCTCTAATTGACAGGCTCATTGCTTTTAAAAATAATGATAATGGAGCTTGGGTCAGAGGAGGAGATA TTATCGTTCAAAATTCAGCATTTGCAGATAATGGAATAGGACTGACCTTTGCCAGTGATGGAAGCTTCCC AAGTGATGAAGGTTCCAGCCAAGAGGTATCTGAATCTCTCTTTGTTGGGGAGAGCAGGAATTACGGCTTT CAGGGTGGTCAGAACAAGTATGTAGGCACTGGAGGAATAGACCAGAAGCCTCGAACATTACCCAGGAACA GGACGTTCCCAATTAGAGGCTTTCAGATTTATGATGGGCCCATTCATCTCACAAGGAGCACTTTCAAAAA ATATGTGCCAACTCCAGATAGGTACAGCAGTGCAATTGGCTTCCTCATGAAGAATTCCTGGCAGATAACC CCCAGGAATAATATCTCCCTCGTGAAGTTTGGTCCACATGTCTCTCTGAATGTCTTTTTTGGAAAGCCTG GTCCCTGGTTTGAAGATTGTGAGATGGATGGTGATAAGAACTCCATATTCCATGACATTGATGGCTCTGT GACAGGATACAAGGATGCTTATGTGGGAAGAATGGACAACTACCTGATCCGCCATCCAAGCTGTGTAAAT GTGTCTAAGTGGAATGCAGTGATCTGCAGTGGGACCTATGCACAGGTCTATGTACAGACATGGAGCACTC AGAATCTTTCTATGACCATTACACGAGATGAGTATCCGTCCAACCCTATGGTGCTCCGAGGTATTAATCA GAAGGCTGCCTTTCCACAGTACCAGCCTGTCGTCATGCTGGAGAAGGGTTATACCATCCACTGGAATGGG CCGGCACCACGGACTACATTTCTATACCTCGTCAACTTCAACAAGAATGACTGGATTCGAGTTGGCCTTT GCTATCCATCAAACACAAGTTTTCAAGTTACCTTTGGCTATTTGCAGCGGCAGAATGGCTCATTATCCAA AATCGAAGAATATGAGCCTGTGCATTCACTGGAAGAACTGCAAAGAAAGCAATCCGAGAGGAAATTCTAT TTTGACTCCAGCACGGGGTTACTGTTTTTGTATCTCAAAGCCAAAAGCCACAGGCATGGCCACAGTTACT GTTCATCTCAGGGATGTGAAAGAGTCAAGATCCAAGCAGCCACAGACTCAAAGGACATCAGTAACTGCAT GGCCAAAGCATACCCACAGTACTACAGAAAGCCGTCAGTGGTCAAGCGGATGCCGGCCATGCTCACTGGA CTCTGTCAAGGCTGTGGCACTCGGCAGGTGGTGTTTACTAGTGATCCTCATAAAAGTTACCTCCCTGTGC AATTCCAGTCACCTGATAAAGCAGAAACCCAGCGTGGAGACCCGTCTGTTATTTCTGTCAATGGCACTGA CTTTACCTTCCGAAGTGCAGGCGTCCTCCTCCTTGTTGTGGATCCGTGCAGCGTTCCATTCCGCTTGACG GAAAAAACGGTTTTTCCTCTTGCTGATGTCAGTCGCATTGAAGAGTATTTAAAAACAGGCATCCCTCCAA GGTCCATTGTTCTGTTGAGCACAAGAGGAGAAATAAAGCAGTTAAACATTTCACACTTACTAGTACCTCT GGGATTAGCCAAACCAGCTCATCTTTATGACAAAGGGAGTACCATATTTTTGGGATTCAGTGGAAACTTT AAACCATCATGGACTAAGCTATTTACCAGTCCTGCTGGACAGGGCCTTGGGGTGCTTGAACAATTCATAC CTTTGCAGCTGGACGAATATGGTTGTCCCAGAGCCACCACTGTCCGCAGAAGAGACCTGGAACTGCTAAA GCAAGCTTCAAAAGCACAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224793 representing NM_013390
Red=Cloning site Green=Tags(s) MYATDSRGHSPAFLQPQNGNSRHPSGYVPGKVVPLRPPPPPKSQASAKFTSIRREDRATFAFSPEEQQAQ RESQKQKRHKNTFICFAITSFSFFIALAIILGISSKYAPDENCPDQNPRLRNWDPGQDSAKQVVIKEGDM LRLTSDATVHSIVIQDGGLLVFGDNKDGSRNITLRTHYILIQDGGALHIGAEKCRYKSKATITLYGKSDE GESMPTFGKKFIGVEAGGTLELHGARKASWTLLARTLNSSGLPFGSYTFEKDFSRGLNVRVIDQDTAKIL ESERFDTHEYRNESRRLQEFLRFQDPGRIVAIAVGDSAAKSLLQGTIQMIQERLGSELIQGLGYRQAWAL VGVIDGGSTSCNESVRNYENHSSGGKALAQREFYTVDGQKFSVTAYSEWIEGVSLSGFRVEVVDGVKLNL LDDVSSWKPGDQIVVASTDYSMYQAEEFTLLPCSECSHFQVKVKETPQFLHMGEIIDGVDMRAEVGILTR NIVIQGEVEDSCYAENQCQFFDYDTFGGHIMIMKNFTSVHLSYVELKHMGQQQMGRYPVHFHLCGDVDYK GGYRHATFVDGLSIHHSFSRCITVHGTNGLLIKDTIGFDTLGHCFFLEDGIEQRNTLFHNLGLLTKPGTL LPTDRNNSMCTTMRDKVFGNYIPVPATDCMAVSTFWIAHPNNNLINNAAAGSQDAGIWYLFHKEPTGESS GLQLLAKPELTPLGIFYNNRVHSNFKAGLFIDKGVKTTNSSAADPREYLCLDNSARFRPHQDANPEKPRV AALIDRLIAFKNNDNGAWVRGGDIIVQNSAFADNGIGLTFASDGSFPSDEGSSQEVSESLFVGESRNYGF QGGQNKYVGTGGIDQKPRTLPRNRTFPIRGFQIYDGPIHLTRSTFKKYVPTPDRYSSAIGFLMKNSWQIT PRNNISLVKFGPHVSLNVFFGKPGPWFEDCEMDGDKNSIFHDIDGSVTGYKDAYVGRMDNYLIRHPSCVN VSKWNAVICSGTYAQVYVQTWSTQNLSMTITRDEYPSNPMVLRGINQKAAFPQYQPVVMLEKGYTIHWNG PAPRTTFLYLVNFNKNDWIRVGLCYPSNTSFQVTFGYLQRQNGSLSKIEEYEPVHSLEELQRKQSERKFY FDSSTGLLFLYLKAKSHRHGHSYCSSQGCERVKIQAATDSKDISNCMAKAYPQYYRKPSVVKRMPAMLTG LCQGCGTRQVVFTSDPHKSYLPVQFQSPDKAETQRGDPSVISVNGTDFTFRSAGVLLLVVDPCSVPFRLT EKTVFPLADVSRIEEYLKTGIPPRSIVLLSTRGEIKQLNISHLLVPLGLAKPAHLYDKGSTIFLGFSGNF KPSWTKLFTSPAGQGLGVLEQFIPLQLDEYGCPRATTVRRRDLELLKQASKAH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013390 |
| ORF Size | 4149 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013390.3 |
| RefSeq Size | 6147 bp |
| RefSeq ORF | 4152 bp |
| Locus ID | 23670 |
| UniProt ID | Q9UHN6 |
| Protein Families | Transmembrane |
| MW | 154.2 kDa |
| Gene Summary | This gene encodes a type II transmembrane protein that belongs to the interferon-induced transmembrane (IFITM) protein superfamily. The encoded protein functions as a cell surface hyaluronidase that cleaves extracellular high molecular weight hyaluronan into intermediate size fragments before internalization and degradation in the lysosome. It also has an interferon-mediated antiviral function in humans through activation of the JAK STAT signaling pathway. The activation of this gene by transcription factor SOX4 in breast cancer cells has been shown to mediate the pathological effects of SOX4 on cancer progression. Naturally occurring mutations in this gene are associated with autosomal recessive non-syndromic hearing loss. [provided by RefSeq, Mar 2017] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Human TMEM2 is not a catalytic hyaluronidase, but a regulator of hyaluronan metabolism via HYBID (KIAA1199/CEMIP) and HAS2 expression
,null,
The Journal of Biological Chemistry
,PubMed ID 37196767
[CEMIP2]
|
|
Implication of cell migration inducing hyaluronidase 1 (CEMIP) in hyaluronan degradation by synovial fibroblasts in patients with knee osteoarthritis
,Shiozawa, J;de Vega, S;Cilek, MZ;Yoshinaga, C;Nakamura, T;Kasamatsu, S;Yoshida, H;Kaneko, H;Ishijima, M;Kaneko, K;Okada, Y;,
Am. J. Pathol.
,PubMed ID 32084364
[CEMIP2]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224793L3 | Lenti ORF clone of Human transmembrane protein 2 (TMEM2), transcript variant 1, Myc-DDK-tagged |
CNY 15,680.00 |
|
| RC224793L4 | Lenti ORF clone of Human transmembrane protein 2 (TMEM2), transcript variant 1, mGFP tagged |
CNY 15,680.00 |
|
| RG224793 | TMEM2 (tGFP-tagged) - Human transmembrane protein 2 (TMEM2), transcript variant 1 |
CNY 15,200.00 |
|
| SC115244 | TMEM2 (untagged)-Human transmembrane protein 2 (TMEM2), transcript variant 1 |
CNY 9,472.00 |
