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NDRG1 (NM_001135242) Human Tagged ORF Clone
CAT＃: RC225609
NDRG1 (Myc-DDK-tagged)-Human N-myc downstream regulated 1 (NDRG1), transcript variant 1
ORF Plasmid: tGFP
"NM_001135242" in other vectors (6)
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CNY 5,488.00
| Cited in 4 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CAP43; CMT4D; DRG-1; DRG1; GC4; HMSNL; NDR1; NMSL; PROXY1; RIT42; RTP; TARG1; TDD5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC225609 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCGGGAGATGCAGGATGTAGACCTCGCTGAGGTGAAGCCTTTGGTGGAGAAAGGGGAGACCATCA CCGGCCTCCTGCAAGAGTTTGATGTCCAGGAGCAGGACATCGAGACTTTACATGGCTCTGTTCACGTCAC GCTGTGTGGGACTCCCAAGGGAAACCGGCCTGTCATCCTCACCTACCATGACATCGGCATGAACCACAAA ACCTGCTACAACCCCCTCTTCAACTACGAGGACATGCAGGAGATCACCCAGCACTTTGCCGTCTGCCACG TGGACGCCCCTGGCCAGCAGGACGGCGCAGCCTCCTTCCCCGCAGGGTACATGTACCCCTCCATGGATCA GCTGGCTGAAATGCTTCCTGGAGTCCTTCAACAGTTTGGGCTGAAAAGCATTATTGGCATGGGAACAGGA GCAGGCGCCTACATCCTAACTCGATTTGCTCTAAACAACCCTGAGATGGTGGAGGGCCTTGTCCTTATCA ACGTGAACCCTTGTGCGGAAGGCTGGATGGACTGGGCCGCCTCCAAGATCTCAGGATGGACCCAAGCTCT GCCGGACATGGTGGTGTCCCACCTTTTTGGGAAGGAAGAAATGCAGAGTAACGTGGAAGTGGTCCACACC TACCGCCAGCACATTGTGAATGACATGAACCCCGGCAACCTGCACCTGTTCATCAATGCCTACAACAGCC GGCGCGACCTGGAGATTGAGCGACCAATGCCGGGAACCCACACAGTCACCCTGCAGTGCCCTGCTCTGTT GGTGGTTGGGGACAGCTCGCCTGCAGTGGATGCCGTGGTGGAGTGCAACTCAAAATTGGACCCAACAAAG ACCACTCTCCTCAAGATGGCGGACTGTGGCGGCCTCCCGCAGATCTCCCAGCCGGCCAAGCTCGCTGAGG CCTTCAAGTACTTCGTGCAGGGCATGGGATACATGCCCTCGGCTAGCATGACCCGCCTGATGCGGTCCCG CACAGCCTCTGGTTCCAGCGTCACTTCTCTGGATGGCACCCGCAGCCGCTCCCACACCAGCGAGGGCACC CGAAGCCGCTCCCACACCAGCGAGGGCACCCGCAGCCGCTCGCACACCAGCGAGGGGGCCCACCTGGACA TCACCCCCAACTCGGGTGCTGCTGGGAACAGCGCCGGGCCCAAGTCCATGGAGGTCTCCTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC225609 protein sequence
Red=Cloning site Green=Tags(s) MSREMQDVDLAEVKPLVEKGETITGLLQEFDVQEQDIETLHGSVHVTLCGTPKGNRPVILTYHDIGMNHK TCYNPLFNYEDMQEITQHFAVCHVDAPGQQDGAASFPAGYMYPSMDQLAEMLPGVLQQFGLKSIIGMGTG AGAYILTRFALNNPEMVEGLVLINVNPCAEGWMDWAASKISGWTQALPDMVVSHLFGKEEMQSNVEVVHT YRQHIVNDMNPGNLHLFINAYNSRRDLEIERPMPGTHTVTLQCPALLVVGDSSPAVDAVVECNSKLDPTK TTLLKMADCGGLPQISQPAKLAEAFKYFVQGMGYMPSASMTRLMRSRTASGSSVTSLDGTRSRSHTSEGT RSRSHTSEGTRSRSHTSEGAHLDITPNSGAAGNSAGPKSMEVSC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001135242 |
| ORF Size | 1182 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001135242.2 |
| RefSeq Size | 3520 bp |
| RefSeq ORF | 1185 bp |
| Locus ID | 10397 |
| UniProt ID | Q92597 |
| MW | 42.8 kDa |
| Gene Summary | This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. The encoded protein is necessary for p53-mediated caspase activation and apoptosis. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012] |
Citations (4)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Usefulness of an immunohistochemical score in advanced pancreatic neuroendocrine tumors treated with CAPTEM or everolimus
,Viúdez, A;Crespo, G;Gómez Dorronsoro, ML;Arozarena, I;Marín-Méndez, JJ;Custodio, A;Benavent, M;Goñi, S;García-Paredes, B;Hernando, J;Durantez, M;Alonso, V;Riesco, MDC;López, C;Jiménez-Fonseca, P;San Vicente, BL;González-Borja, I;Sevilla, I;Hernández-Garcia, I;Carmona-Bayonas, A;Capdevila, J;Pérez-Sanz, J;García-Carbonero, R;Pérez-Ricarte, L;Llanos, M;Vera, R;De Jesús Acosta, A;,
Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.] 2020
,PubMed ID 33358592
[NDRG1]
|
|
The proneural gene ASCL1 governs the transcriptional subgroup affiliation in glioblastoma stem cells by directly repressing the mesenchymal gene NDRG1
,Narayanan, A;Gagliardi, F;Gallotti, AL;Mazzoleni, S;Cominelli, M;Fagnocchi, L;Pala, M;Piras, IS;Zordan, P;Moretta, N;Tratta, E;Brugnara, G;Altabella, L;Bozzuto, G;Gorombei, P;Molinari, A;Padua, RA;Bulfone, A;Politi, LS;Falini, A;Castellano, A;Mortini, P;Zippo, A;Poliani, PL;Galli, R;,
Cell Death Differ.
,PubMed ID 30538287
[NDRG1]
|
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N-Myc Downstream-Regulated Gene 1 Restricts Hepatitis C Virus Propagation by Regulating Lipid Droplet Biogenesis and Viral Assembly
,Schweitzer, CJ;Zhang, F;Boyer, A;Valdez, K;Cam, M;Liang, TJ;,
J. Virol.2017
,PubMed ID 29118118
[NDRG1]
|
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SET-mediated NDRG1 inhibition is involved in acquisition of epithelial-to-mesenchymal transition phenotype and cisplatin resistance in human lung cancer cell
,Liu, H;Gu, Y;Yin, J;Zheng, G;Wang, C;Zhang, Z;Deng, M;Liu, J;Jia, X;He, Z;,
Cell. Signal.
,PubMed ID 25152373
[NDRG1]
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Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC225609L1 | Lenti ORF clone of Human N-myc downstream regulated 1 (NDRG1), transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC225609L2 | Lenti ORF clone of Human N-myc downstream regulated 1 (NDRG1), transcript variant 1, mGFP tagged |
CNY 7,888.00 |
|
| RC225609L3 | Lenti ORF clone of Human N-myc downstream regulated 1 (NDRG1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC225609L4 | Lenti ORF clone of Human N-myc downstream regulated 1 (NDRG1), transcript variant 1, mGFP tagged |
CNY 7,888.00 |
|
| RG225609 | NDRG1 (tGFP-tagged) - Human N-myc downstream regulated 1 (NDRG1), transcript variant 1 |
CNY 4,370.00 |
|
| SC325042 | NDRG1 (untagged)-Human N-myc downstream regulated 1 (NDRG1), transcript variant 1 |
CNY 5,488.00 |
