SLC6A12 (NM_001122847) Human Tagged ORF Clone
CAT#: RC226025
SLC6A12 (Myc-DDK-tagged)-Human solute carrier family 6 (neurotransmitter transporter, betaine/GABA), member 12 (SLC6A12), transcript variant 2
ORF Plasmid: tGFP
"NM_001122847" in other vectors (4)
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CNY 6,856.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | BGT-1; BGT1; GAT2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC226025 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGGGAAGGTGGCAGTGCAAGAGCGTGGGCCTCCTGCGGTCTCCTGGGTCCCCGAGGAGGGAGAGA AGTTGGACCAGGAAGACGAGGACCAGGTGAAGGATCGGGGCCAATGGACCAACAAGATGGAGTTTGTGCT GTCAGTGGCCGGGGAGATCATTGGGCTGGGCAATGTCTGGAGGTTTCCCTATCTCTGCTACAAAAACGGA GGTGGAGCCTTCTTCATCCCCTACTTCATCTTCTTCTTTGTCTGCGGCATCCCGGTGTTCTTCCTGGAGG TGGCGTTGGGCCAATACACCAGCCAAGGGAGTGTCACAGCCTGGAGGAAGATCTGCCCCCTCTTCCAGGG CATTGGTCTGGCATCTGTGGTCATCGAGTCATATTTGAATGTCTACTACATCATCATCCTTGCCTGGGCT CTCTTCTACCTGTTCAGCTCCTTCACTTCTGAGCTGCCCTGGACGACCTGCAACAACTTTTGGAACACAG AGCATTGCACGGACTTTCTGAACCACTCAGGAGCCGGCACAGTGACCCCATTTGAGAATTTTACCTCACC TGTCATGGAATTCTGGGAGAGACGAGTTCTGGGCATCACCTCGGGCATCCATGACCTGGGCTCCCTGCGC TGGGAGCTGGCCCTGTGCCTCCTGCTCGCCTGGGTCATCTGCTATTTCTGCATCTGGAAGGGGGTCAAGT CCACAGGCAAGGTGGTTTATTTCACAGCCACGTTTCCGTACCTGATGCTTGTCATTTTGCTGATCAGAGG TGTCACCCTTCCCGGAGCCTACCAGGGCATCATCTACTACTTGAAGCCAGATTTGTTCCGCCTCAAGGAC CCTCAGGTGTGGATGGATGCGGGCACCCAGATCTTCTTCTCCTTTGCCATCTGCCAGGGGTGCCTGACAG CCCTGGGCAGCTACAACAAGTATCACAACAACTGCTACAAGGACTGCATCGCCCTCTGCTTCCTGAACAG TGCCACCAGCTTTGTGGCTGGGTTTGTTGTCTTCTCCATCCTGGGCTTCATGTCCCAAGAGCAAGGGGTG CCCATTTCTGAAGTGGCCGAGTCAGGTCCTGGGCTGGCCTTCATCGCCTTCCCCAAGGCTGTGACTATGA TGCCCTTATCCCAGCTGTGGTCCTGCCTGTTCTTTATCATGCTCATATTCCTAGGGCTGGACAGCCAGTT TGTCTGTGTGGAGTGCCTGGTGACAGCCTCCATAGACATGTTCCCCAGGCAGCTCCGGAAGAGCGGGCGG CGCGAGCTCCTCATCCTCACCATCGCCGTCATGTGCTACCTGATAGGGCTTTTCCTGGTCACCGAGGGCG GGATGTACATCTTCCAGCTGTTTGACTACTATGCTTCCAGTGGCATATGCCTGCTGTTCCTGTCATTGTT TGAAGTGGTCTGCATAAGCTGGGTGTATGGGGCGGACCGTTTCTATGACAACATTGAGGACATGATTGGC TACCGGCCATGGCCCCTGGTGAAGATCTCCTGGCTCTTCCTGACCCCTGGACTTTGCCTGGCCACTTTCC TCTTCTCCTTGAGCAAGTACACCCCCCTCAAGTACAACAACGTCTATGTGTACCCGCCCTGGGGATACTC CATTGGCTGGTTCCTGGCTCTGTCCTCCATGGTCTGTGTCCCACTCTTCGTCGTCATCACCCTCCTGAAG ACTCGGGGTCCTTTCAGGAAGCGTCTGCGTCAGCTCATCACCCCTGACTCCAGTCTGCCACAGCCCAAGC AACATCCCTGCTTGGATGGCAGTGCTGGCCGGAACTTTGGGCCCTCCCCAACAAGGGAAGGACTGATAGC CGGGGAGAAGGAGACCCATTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC226025 protein sequence
Red=Cloning site Green=Tags(s) MDGKVAVQERGPPAVSWVPEEGEKLDQEDEDQVKDRGQWTNKMEFVLSVAGEIIGLGNVWRFPYLCYKNG GGAFFIPYFIFFFVCGIPVFFLEVALGQYTSQGSVTAWRKICPLFQGIGLASVVIESYLNVYYIIILAWA LFYLFSSFTSELPWTTCNNFWNTEHCTDFLNHSGAGTVTPFENFTSPVMEFWERRVLGITSGIHDLGSLR WELALCLLLAWVICYFCIWKGVKSTGKVVYFTATFPYLMLVILLIRGVTLPGAYQGIIYYLKPDLFRLKD PQVWMDAGTQIFFSFAICQGCLTALGSYNKYHNNCYKDCIALCFLNSATSFVAGFVVFSILGFMSQEQGV PISEVAESGPGLAFIAFPKAVTMMPLSQLWSCLFFIMLIFLGLDSQFVCVECLVTASIDMFPRQLRKSGR RELLILTIAVMCYLIGLFLVTEGGMYIFQLFDYYASSGICLLFLSLFEVVCISWVYGADRFYDNIEDMIG YRPWPLVKISWLFLTPGLCLATFLFSLSKYTPLKYNNVYVYPPWGYSIGWFLALSSMVCVPLFVVITLLK TRGPFRKRLRQLITPDSSLPQPKQHPCLDGSAGRNFGPSPTREGLIAGEKETHL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001122847 |
ORF Size | 1842 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001122847.3 |
RefSeq Size | 3165 bp |
RefSeq ORF | 1845 bp |
Locus ID | 6539 |
UniProt ID | P48065 |
Protein Families | Druggable Genome, Transmembrane |
MW | 69.4 kDa |
Gene Summary | Transports betaine and GABA. May have a role in regulation of GABAergic transmission in the brain through the reuptake of GABA into presynaptic terminals, as well as in osmotic regulation.[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Astrocytic γ-aminobutyric acid (GABA) transporters mediate guanidinoacetate transport in rat brain
,Tachikawa, M;Yashiki, A;Akanuma, SI;Matsukawa, H;Ide, S;Minami, M;Hosoya, KI;,
Neurochem. Int.2017
,PubMed ID 29175673
[SLC6A12]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC226025L3 | Lenti ORF clone of Human solute carrier family 6 (neurotransmitter transporter, betaine/GABA), member 12 (SLC6A12), transcript variant 2, Myc-DDK-tagged |
CNY 6,460.00 |
|
RC226025L4 | Lenti ORF clone of Human solute carrier family 6 (neurotransmitter transporter, betaine/GABA), member 12 (SLC6A12), transcript variant 2, mGFP tagged |
CNY 6,460.00 |
|
RG226025 | SLC6A12 (tGFP-tagged) - Human solute carrier family 6 (neurotransmitter transporter, betaine/GABA), member 12 (SLC6A12), transcript variant 2 |
CNY 5,040.00 |
|
SC318954 | SLC6A12 (untagged)-Human solute carrier family 6 (neurotransmitter transporter, betaine/GABA), member 12 (SLC6A12), transcript variant 2 |
CNY 6,864.00 |
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