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Metabotropic Glutamate Receptor 8 (GRM8) (NM_001127323) Human Tagged ORF Clone
CAT＃: RC226256
GRM8 (Myc-DDK-tagged)-Human glutamate receptor, metabotropic 8 (GRM8), transcript variant 2
ORF Plasmid: tGFP
"NM_001127323" in other vectors (4)
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CNY 6,648.00
CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GLUR8; GPRC1H; mGlu8; MGLUR8 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC226256 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTATGCGAGGGAAAGCGATCAGCCTCTTGCCCTTGTTTCTTCCTCTTGACCGCCAAGTTCTACTGGA TCCTCACAATGATGCAAAGAACTCACAGCCAGGAGTATGCCCATTCCATACGGGTGGATGGGGACATTAT TTTGGGGGGTCTCTTCCCTGTCCACGCAAAGGGAGAGAGAGGGGTGCCTTGTGGGGAGCTGAAGAAGGAA AAGGGGATTCACAGACTGGAGGCCATGCTTTATGCAATTGACCAGATTAACAAGGACCCTGATCTCCTTT CCAACATCACTCTGGGTGTCCGCATCCTCGACACGTGCTCTAGGGACACCTATGCTTTGGAGCAGTCTCT AACATTCGTGCAGGCATTAATAGAGAAAGATGCTTCGGATGTGAAGTGTGCTAATGGAGATCCACCCATT TTCACCAAGCCCGACAAGATTTCTGGCGTCATAGGTGCTGCAGCAAGCTCCGTGTCCATCATGGTTGCTA ACATTTTAAGACTTTTTAAGATACCTCAAATCAGCTATGCATCCACAGCCCCAGAGCTAAGTGATAACAC CAGGTATGACTTTTTCTCTCGAGTGGTTCCGCCTGACTCCTACCAAGCCCAAGCCATGGTGGACATCGTG ACAGCACTGGGATGGAATTATGTTTCGACACTGGCTTCTGAGGGGAACTATGGTGAGAGCGGTGTGGAGG CCTTCACCCAGATCTCGAGGGAGATTGGTGGTGTTTGCATTGCTCAGTCACAGAAAATCCCACGTGAACC AAGACCTGGAGAATTTGAAAAAATTATCAAACGCCTGCTAGAAACACCTAATGCTCGAGCAGTGATTATG TTTGCCAATGAGGATGACATCAGGAGGATATTGGAAGCAGCAAAAAAACTAAACCAAAGTGGGCATTTTC TCTGGATTGGCTCAGATAGTTGGGGATCCAAAATAGCACCTGTCTATCAGCAAGAGGAGATTGCAGAAGG GGCTGTGACAATTTTGCCCAAACGAGCATCAATTGATGGATTTGATCGATACTTTAGAAGCCGAACTCTT GCCAATAATCGAAGAAATGTGTGGTTTGCAGAATTCTGGGAGGAGAATTTTGGCTGCAAGTTAGGATCAC ATGGGAAAAGGAACAGTCATATAAAGAAATGCACAGGGCTGGAGCGAATTGCTCGGGATTCATCTTATGA ACAGGAAGGAAAGGTCCAATTTGTAATTGATGCTGTATATTCCATGGCTTACGCCCTGCACAATATGCAC AAAGATCTCTGCCCTGGATACATTGGCCTTTGTCCACGAATGAGTACCATTGATGGGAAAGAGCTACTTG GTTATATTCGGGCTGTAAATTTTAATGGCAGTGCTGGCACTCCTGTCACTTTTAATGAAAACGGAGATGC TCCTGGACGTTATGATATCTTCCAGTATCAAATAACCAACAAAAGCACAGAGTACAAAGTCATCGGCCAC TGGACCAATCAGCTTCATCTAAAAGTGGAAGACATGCAGTGGGCTCATAGAGAACATACTCACCCGGCGT CTGTCTGCAGCCTGCCGTGTAAGCCAGGGGAGAGGAAGAAAACGGTGAAAGGGGTCCCTTGCTGCTGGCA CTGTGAACGCTGTGAAGGTTACAACTACCAGGTGGATGAGCTGTCCTGTGAACTTTGCCCTCTGGATCAG AGACCCAACATGAACCGCACAGGCTGCCAGCTTATCCCCATCATCAAATTGGAGTGGCATTCTCCCTGGG CTGTGGTGCCTGTGTTTGTTGCAATATTGGGAATCATCGCCACCACCTTTGTGATCGTGACCTTTGTCCG CTATAATGACACACCTATCGTGAGGGCTTCAGGACGCGAACTTAGTTACGTGCTCCTAACGGGGATTTTT CTCTGTTATTCAATCACGTTTTTAATGATTGCAGCACCAGATACAATCATATGCTCCTTCCGACGGGTCT TCCTAGGACTTGGCATGTGTTTCAGCTATGCAGCCCTTCTGACCAAAACAAACCGTATCCACCGAATATT TGAGCAGGGGAAGAAATCTGTCACAGCGCCCAAGTTCATTAGTCCAGCATCTCAGCTGGTGATCACCTTC AGCCTCATCTCCGTCCAGCTCCTTGGAGTGTTTGTCTGGTTTGTTGTGGATCCCCCCCACATCATCATTG ACTATGGAGAGCAGCGGACACTAGATCCAGAGAAGGCCAGGGGAGTGCTCAAGTGTGACATTTCTGATCT CTCACTCATTTGTTCACTTGGATACAGTATCCTCTTGATGGTCACTTGTACTGTTTATGCCATTAAAACG AGAGGTGTCCCAGAGACTTTCAATGAAGCCAAACCTATTGGATTTACCATGTATACCACCTGCATCATTT GGTTAGCTTTCATCCCCATCTTTTTTGGTACAGCCCAGTCAGCAGAAAAGATGTACATCCAGACAACAAC ACTTACTGTCTCCATGAGTTTAAGTGCTTCAGTATCTCTGGGCATGCTCTATATGCCCAAGGTTTATATT ATAATTTTTCATCCAGAACAGAATGTTCAAAAACGCAAGAGGAGCTTCAAGGCTGTGGTGACAGCTGCCA CCATGCAAAGCAAACTGATCCAAAAAGGAAATGACAGACCAAATGGCGAGGTGAAAAGTGAACTCTGTGA GAGTCTTGAAACCAACACTTCCTCTACCAAGACAACATATATCAGTTACAGCAATCATTCAATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC226256 protein sequence
Red=Cloning site Green=Tags(s) MVCEGKRSASCPCFFLLTAKFYWILTMMQRTHSQEYAHSIRVDGDIILGGLFPVHAKGERGVPCGELKKE KGIHRLEAMLYAIDQINKDPDLLSNITLGVRILDTCSRDTYALEQSLTFVQALIEKDASDVKCANGDPPI FTKPDKISGVIGAAASSVSIMVANILRLFKIPQISYASTAPELSDNTRYDFFSRVVPPDSYQAQAMVDIV TALGWNYVSTLASEGNYGESGVEAFTQISREIGGVCIAQSQKIPREPRPGEFEKIIKRLLETPNARAVIM FANEDDIRRILEAAKKLNQSGHFLWIGSDSWGSKIAPVYQQEEIAEGAVTILPKRASIDGFDRYFRSRTL ANNRRNVWFAEFWEENFGCKLGSHGKRNSHIKKCTGLERIARDSSYEQEGKVQFVIDAVYSMAYALHNMH KDLCPGYIGLCPRMSTIDGKELLGYIRAVNFNGSAGTPVTFNENGDAPGRYDIFQYQITNKSTEYKVIGH WTNQLHLKVEDMQWAHREHTHPASVCSLPCKPGERKKTVKGVPCCWHCERCEGYNYQVDELSCELCPLDQ RPNMNRTGCQLIPIIKLEWHSPWAVVPVFVAILGIIATTFVIVTFVRYNDTPIVRASGRELSYVLLTGIF LCYSITFLMIAAPDTIICSFRRVFLGLGMCFSYAALLTKTNRIHRIFEQGKKSVTAPKFISPASQLVITF SLISVQLLGVFVWFVVDPPHIIIDYGEQRTLDPEKARGVLKCDISDLSLICSLGYSILLMVTCTVYAIKT RGVPETFNEAKPIGFTMYTTCIIWLAFIPIFFGTAQSAEKMYIQTTTLTVSMSLSASVSLGMLYMPKVYI IIFHPEQNVQKRKRSFKAVVTAATMQSKLIQKGNDRPNGEVKSELCESLETNTSSTKTTYISYSNHSI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001127323 |
| ORF Size | 2724 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001127323.1, NP_001120795.1 |
| RefSeq Size | 3873 bp |
| RefSeq ORF | 2727 bp |
| Locus ID | 2918 |
| UniProt ID | O00222 |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| Protein Pathways | Neuroactive ligand-receptor interaction |
| MW | 101.7 kDa |
| Gene Summary | L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226256L3 | Lenti ORF clone of Human glutamate receptor, metabotropic 8 (GRM8), transcript variant 2, Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC226256L4 | Lenti ORF clone of Human glutamate receptor, metabotropic 8 (GRM8), transcript variant 2, mGFP tagged |
CNY 9,030.00 |
|
| RG226256 | GRM8 (tGFP-tagged) - Human glutamate receptor, metabotropic 8 (GRM8), transcript variant 2 |
CNY 7,890.00 |
|
| SC323233 | GRM8 (untagged)-Human glutamate receptor, metabotropic 8 (GRM8), transcript variant 2 |
CNY 7,220.00 |
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