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ARHGEF14 (MCF2L) (NM_001112732) Human Tagged ORF Clone
CAT＃: RC226352
MCF2L (Myc-DDK-tagged)-Human MCF.2 cell line derived transforming sequence-like (MCF2L), transcript variant 1
ORF Plasmid: tGFP
"NM_001112732" in other vectors (6)
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CNY 8,232.00
Product images
CNY 300.00
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ARHGEF14; DBS; OST |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC226352 representing NM_001112732
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAGGGTGGAGAGCCCCGTGCCTGCAGCGCGCGCTTCCCTCACTGGGTCCTGCGTCCTTGGGCAGG CGATGCCCCTGCGGGGAGGGGCTGGTCCATCCCCGGCCAGCCACGGACCCACGCATGGACCCAGCGACCC ACGGACCTGCTTACCTGGGCGCGGCGCGGGTGGCATGCGGCCACACGGAAGGGGCGCGCTGGGCTGCTGC GGCCTCTGCAGCTTCTACACCTGCCACGGGGCGGCCGGAGATGAAATCATGCACCAGGACATCGTCCCGC TCTGTGCTGCCGACATCCAGGACCAGCTAAAGAAGCGCTTTGCTTACCTGTCCGGTGGGCGGGGGCAGGA CGGAAGCCCGGTTATCACCTTCCCTGACTACCCGGCCTTCAGCGAGATTCCGGACAAGGAGTTCCAGAAT GTCATGACCTACCTCACCAGCATCCCCAGCCTGCAGGACGCTGGCATCGGATTCATCCTGGTGATAGACC GGCGACGGGACAAATGGACCTCCGTGAAGGCGTCCGTCCTGCGCATCGCAGCATCTTTCCCGGCAAACCT GCAGCTCGTCCTCGTGCTTCGCCCGACGGGTTTTTTCCAAAGGACTCTCTCCGACATCGCTTTCAAATTC AATAGAGATGACTTTAAGATGAAGGTGCCGGTCATAATGCTGAGCTCCGTACCAGACTTACACGGTTACA TCGATAAGTCGCAGCTGACCGAGGACCTGGGTGGGACCCTGGACTACTGCCACTCCCGGTGGCTGTGCCA GCGCACGGCCATCGAAAGTTTCGCCCTCATGGTGAAGCAGACGGCTCAGATGCTGCAGTCCTTCGGGACC GAGCTGGCTGAAACAGAGCTGCCCAATGACGTCCAGTCGACAAGCTCAGTGCTGTGTGCGCACACAGAGA AGAAGGACAAGGCGAAGGAGGATTTGAGGCTGGCACTGAAAGAGGGGCACAGTGTCCTGGAGAGCCTCAG GGAGCTGCAGGCTGAGGGCTCAGAGCCCAGTGTGAACCAGGACCAGCTTGACAACCAGGCCACCGTGCAG AGGCTCCTGGCCCAGCTGAACGAAACCGAGGCTGCCTTCGATGAGTTCTGGGCAAAGCATCAGCAGAAAC TGGAGCAGTGTCTGCAGCTCCGGCACTTTGAGCAGGGCTTCCGGGAGGTCAAAGCCATCTTGGACGCAGC GTCCCAGAAGATAGCAACCTTCACAGACATCGGCAACAGCCTGGCGCATGTGGAGCACCTGCTGAGGGAC CTGGCCAGCTTCGAGGAGAAATCAGGCGTGGCCGTGGAGAGGGCCCGGGCCCTGTCTCTGGACGGCGAGC AGCTCATTGGGAACAAGCACTACGCGGTAGACTCCATCCGCCCAAAGTGCCAGGAGCTCCGGCACCTCTG TGACCAGTTCTCTGCGGAGATCGCAAGGAGGAGGGGGCTGCTCAGCAAGTCCCTGGAGCTGCACCGCCGC CTGGAGACGTCCATGAAGTGGTGTGATGAAGGGATTTACCTGCTGGCCTCACAACCTGTGGACAAGTGCC AGTCCCAGGACGGCGCGGAGGCTGCCCTCCAGGAAATCGAGAAGTTTTTGGAGACCGGTGCGGAAAATAA GATCCAGGAGCTCAACGCGATTTACAAGGAATACGAATCCATCCTCAACCAAGATCTCATGGAGCACGTG CGAAAGGTCTTCCAGAAGCAGGCAAGCATGGAGGAGGTGTTCCACCGCAGGCAGGCCAGCCTGAAGAAGC TGGCGGCCAGGCAGACGCGGCCCGTGCAGCCGGTGGCCCCCAGACCCGAGGCACTGGCAAAGTCGCCCTG CCCCTCCCCAGGCATTCGGCGAGGCTCTGAGAACTCCAGCTCCGAGGGCGGTGCGCTCCGGAGAGGGCCC TACCGGAGGGCCAAGAGTGAGATGAGTGAGAGCCGGCAGGGCCGCGGCTCAGCGGGGGAGGAGGAGGAAA GCCTGGCCATCCTGCGCAGGCACGTGATGAGCGAGCTCCTGGACACAGAACGGGCCTACGTGGAGGAGCT GCTGTGCGTCCTGGAGGGCTACGCCGCGGAGATGGATAACCCACTGATGGCTCACCTCCTGTCAACAGGC CTTCACAACAAGAAGGATGTTTTGTTTGGAAACATGGAGGAAATCTATCACTTCCACAACAGGATATTCC TCAGGGAGCTGGAAAACTACACTGACTGCCCAGAACTGGTTGGAAGATGCTTTCTGGAGAGGATGGAAGA TTTCCAGATCTATGAGAAGTACTGTCAGAACAAGCCCCGCTCTGAGAGCCTGTGGAGACAGTGCTCCGAC TGCCCGTTTTTCCAGGAATGCCAGAGAAAGCTGGACCACAAGCTGAGCCTGGACTCCTACCTGCTGAAGC CAGTGCAGAGGATCACCAAGTACCAGCTGCTGCTCAAGGAAATGCTGAAATACAGCAGGAACTGCGAGGG GGCTGAGGACCTGCAGGAGGCGCTGAGCTCCATCCTGGGCATCCTGAAGGCCGTGAACGACTCCATGCAC CTCATCGCTATCACCGGCTATGACGGGAATCTCGGCGACCTGGGCAAGCTGCTGATGCAGGGCTCGTTCA GCGTCTGGACCGACCACAAGAGGGGCCACACCAAGGTGAAGGAGCTGGCCAGGTTCAAGCCCATGCAGCG GCACCTGTTCCTGCACGAGAAGGCAGTGCTCTTCTGCAAGAAGAGGGAGGAGAATGGGGAGGGGTATGAG AAAGCTCCCTCCTACAGCTACAAGCAGTCCTTAAACATGGCTGCCGTTGGCATTACGGAGAACGTGAAGG GAGATGCTAAGAAGTTCGAGATCTGGTACAACGCGCGCGAGGAGGTCTACATCGTCCAGGCGCCAACTCC TGAGATTAAAGCCGCGTGGGTGAATGAAATTCGGAAAGTGCTGACCAGCCAGCTGCAGGCTTGTAGAGAA GCCAGCCAGCACCGGGCGCTGGAGCAGTCACAGAGCCTGCCCCTGCCGGCCCCGACCAGCACCAGTCCCT CAAGAGGAAACTCAAGGAACATCAAGAAGCTGGAAGAAAGGAAAACAGACCCCCTAAGCCTGGAGGGATA CGTCAGCTCAGCGCCACTGACAAAGCCCCCCGAAAAGGGCAAAGGTTGGAGCAAAACGTCCCACTCACTG GAGGCACCTGAGGACGACGGGGGCTGGTCAAGTGCAGAGGAGCAGATTAACTCGTCCGACGCAGAGGAGG ACGGCGGGTTGGGCCCCAAGAAGCTGGTTCCAGGTAAATACACGGTCGTGGCGGACCACGAGAAGGGAGG CCCCGATGCGCTGCGCGTGAGGAGCGGGGACGTGGTGGAGCTGGTGCAGGAGGGCGACGAGGGCCTCTGG TACGTCAGGGACCCGACCACTGGCAAGGAGGGCTGGGTGCCGGCCAGCAGCCTGTCCGTCCGGCTCGGCC CGTCCGGCTCGGCCCAGTGCCTGAGCAGCTCAGAGTCGAGCCCGGGGTCGGCCGTGCTGAGCAACTCGTC CAGCTGCAGCGAGGGCGGCCAGGCCCCCTTCTCCGACCTGCAGGGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC226352 representing NM_001112732
Red=Cloning site Green=Tags(s) MARVESPVPAARASLTGSCVLGQAMPLRGGAGPSPASHGPTHGPSDPRTCLPGRGAGGMRPHGRGALGCC GLCSFYTCHGAAGDEIMHQDIVPLCAADIQDQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQN VMTYLTSIPSLQDAGIGFILVIDRRRDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKF NRDDFKMKVPVIMLSSVPDLHGYIDKSQLTEDLGGTLDYCHSRWLCQRTAIESFALMVKQTAQMLQSFGT ELAETELPNDVQSTSSVLCAHTEKKDKAKEDLRLALKEGHSVLESLRELQAEGSEPSVNQDQLDNQATVQ RLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKAILDAASQKIATFTDIGNSLAHVEHLLRD LASFEEKSGVAVERARALSLDGEQLIGNKHYAVDSIRPKCQELRHLCDQFSAEIARRRGLLSKSLELHRR LETSMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNAIYKEYESILNQDLMEHV RKVFQKQASMEEVFHRRQASLKKLAARQTRPVQPVAPRPEALAKSPCPSPGIRRGSENSSSEGGALRRGP YRRAKSEMSESRQGRGSAGEEEESLAILRRHVMSELLDTERAYVEELLCVLEGYAAEMDNPLMAHLLSTG LHNKKDVLFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSD CPFFQECQRKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSRNCEGAEDLQEALSSILGILKAVNDSMH LIAITGYDGNLGDLGKLLMQGSFSVWTDHKRGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYE KAPSYSYKQSLNMAAVGITENVKGDAKKFEIWYNAREEVYIVQAPTPEIKAAWVNEIRKVLTSQLQACRE ASQHRALEQSQSLPLPAPTSTSPSRGNSRNIKKLEERKTDPLSLEGYVSSAPLTKPPEKGKGWSKTSHSL EAPEDDGGWSSAEEQINSSDAEEDGGLGPKKLVPGKYTVVADHEKGGPDALRVRSGDVVELVQEGDEGLW YVRDPTTGKEGWVPASSLSVRLGPSGSAQCLSSSESSPGSAVLSNSSSCSEGGQAPFSDLQG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001112732 |
| ORF Size | 3546 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001112732.1, NP_001106203.1 |
| RefSeq ORF | 3378 bp |
| Locus ID | 23263 |
| UniProt ID | O15068 |
| MW | 131.2 kDa |
| Gene Summary | This gene encodes a guanine nucleotide exchange factor that interacts specifically with the GTP-bound Rac1 and plays a role in the Rho/Rac signaling pathways. A variant in this gene was associated with osteoarthritis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226352L1 | Lenti ORF clone of Human MCF.2 cell line derived transforming sequence-like (MCF2L), transcript variant 1, Myc-DDK-tagged |
CNY 10,632.00 |
|
| RC226352L2 | Lenti ORF clone of Human MCF.2 cell line derived transforming sequence-like (MCF2L), transcript variant 1, mGFP tagged |
CNY 13,680.00 |
|
| RC226352L3 | Lenti ORF clone of Human MCF.2 cell line derived transforming sequence-like (MCF2L), transcript variant 1, Myc-DDK-tagged |
CNY 13,680.00 |
|
| RC226352L4 | Lenti ORF clone of Human MCF.2 cell line derived transforming sequence-like (MCF2L), transcript variant 1, mGFP tagged |
CNY 13,680.00 |
|
| RG226352 | MCF2L (tGFP-tagged) - Human MCF.2 cell line derived transforming sequence-like (MCF2L), transcript variant 1 |
CNY 12,920.00 |
|
| SC319026 | MCF2L (untagged)-Human MCF.2 cell line derived transforming sequence-like (MCF2L), transcript variant 1 |
CNY 22,710.00 |
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