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DNMT1 (NM_001130823) Human Tagged ORF Clone
CAT＃: RC226414
- TrueORF®
DNMT1 (Myc-DDK-tagged)-Human DNA (cytosine-5-)-methyltransferase 1 (DNMT1), transcript variant 1
ORF Plasmid: tGFP
"NM_001130823" in other vectors (6)
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CNY 16,752.00
| Cited in 5 publications. |
Product images
CNY 300.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ADCADN; AIM; CXXC9; DNMT; HSN1E; m.HsaI; MCMT |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC226414 representing NM_001130823.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCCGGCGCGTACCGCCCCAGCCCGGGTGCCCACACTGGCCGTCCCGGCCATCTCGCTGCCCGACGAT GTCCGCAGGCGGCTCAAAGATTTGGAAAGAGACAGCTTAACAGAAAAGGAATGTGTGAAGGAGAAATTG AATCTCTTGCACGAATTTCTGCAAACAGAAATAAAGAATCAGTTATGTGACTTGGAAACCAAATTACGT AAAGAAGAATTATCCGAGGAGGGCTACCTGGCTAAAGTCAAATCCCTTTTAAATAAAGATTTGTCCTTG GAGAACGGTGCTCATGCTTACAACCGGGAAGTGAATGGACGTCTAGAAAACGGGAACCAAGCAAGAAGT GAAGCCCGTAGAGTGGGAATGGCAGATGCCAACAGCCCCCCCAAACCCCTTTCCAAACCTCGCACGCCC AGGAGGAGCAAGTCCGATGGAGAGGCTAAGCGTTCAAGAGACCCTCCTGCCTCAGCCTCCCAAGTAACT GGGATTAGAGCTGAACCTTCACCTAGCCCCAGGATTACAAGGAAAAGCACCAGGCAAACCACCATCACA TCTCATTTTGCAAAGGGCCCTGCCAAACGGAAACCTCAGGAAGAGTCTGAAAGAGCCAAATCGGATGAG TCCATCAAGGAAGAAGACAAAGACCAGGATGAGAAGAGACGTAGAGTTACATCCAGAGAACGAGTTGCT AGACCGCTTCCTGCAGAAGAACCTGAAAGAGCAAAATCAGGAACGCGCACTGAAAAGGAAGAAGAAAGA GATGAAAAAGAAGAAAAGAGACTCCGAAGTCAAACCAAAGAACCAACACCCAAACAGAAACTGAAGGAG GAGCCGGACAGAGAAGCCAGGGCAGGCGTGCAGGCTGACGAGGACGAAGATGGAGACGAGAAAGATGAG AAGAAGCACAGAAGTCAACCCAAAGATCTAGCTGCCAAACGGAGGCCCGAAGAAAAAGAACCTGAAAAA GTAAATCCACAGATTTCTGATGAAAAAGACGAGGATGAAAAGGAGGAGAAGAGACGCAAAACGACCCCC AAAGAACCAACGGAGAAAAAAATGGCTCGCGCCAAAACAGTCATGAACTCCAAGACCCACCCTCCCAAG TGCATTCAGTGCGGGCAGTACCTGGACGACCCTGACCTCAAATATGGGCAGCACCCACCAGACGCGGTG GATGAGCCACAGATGCTGACAAATGAGAAGCTGTCCATCTTTGATGCCAACGAGTCTGGCTTTGAGAGT TATGAGGCGCTTCCCCAGCACAAACTGACCTGCTTCAGTGTGTACTGTAAGCACGGTCACCTGTGTCCC ATCGACACCGGCCTCATCGAGAAGAATATCGAACTCTTCTTTTCTGGTTCAGCAAAACCAATCTATGAT GATGACCCGTCTCTTGAAGGTGGTGTTAATGGCAAAAATCTTGGCCCCATAAATGAATGGTGGATCACT GGCTTTGATGGAGGTGAAAAGGCCCTCATCGGCTTCAGCACCTCATTTGCCGAATACATTCTGATGGAT CCCAGTCCCGAGTATGCGCCCATATTTGGGCTGATGCAGGAGAAGATCTACATCAGCAAGATTGTGGTG GAGTTCCTGCAGAGCAATTCCGACTCGACCTATGAGGACCTGATCAACAAGATCGAGACCACGGTTCCT CCTTCTGGCCTCAACTTGAACCGCTTCACAGAGGACTCCCTCCTGCGACACGCGCAGTTTGTGGTGGAG CAGGTGGAGAGTTATGACGAGGCCGGGGACAGTGATGAGCAGCCCATCTTCCTGACGCCCTGCATGCGG GACCTGATCAAGCTGGCTGGGGTCACGCTGGGACAGAGGCGAGCCCAGGCGAGGCGGCAGACCATCAGG CATTCTACCAGGGAGAAGGACAGGGGACCCACGAAAGCCACCACCACCAAGCTGGTCTACCAGATCTTC GATACTTTCTTCGCAGAGCAAATTGAAAAGGATGACAGAGAAGACAAGGAGAACGCCTTTAAGCGCCGG CGATGTGGCGTCTGTGAGGTGTGTCAGCAGCCTGAGTGTGGGAAATGTAAAGCCTGCAAGGACATGGTT AAATTTGGTGGCAGTGGACGGAGCAAGCAGGCTTGCCAAGAGCGGAGGTGTCCCAATATGGCCATGAAG GAGGCAGATGACGATGAGGAAGTCGATGATAACATCCCAGAGATGCCGTCACCCAAAAAAATGCACCAG GGGAAGAAGAAGAAACAGAACAAGAATCGCATCTCTTGGGTCGGAGAAGCCGTCAAGACTGATGGGAAG AAGAGTTACTATAAGAAGGTGTGCATTGATGCGGAAACCCTGGAAGTGGGGGACTGTGTCTCTGTTATT CCAGATGATTCCTCAAAACCGCTGTATCTAGCAAGGGTCACGGCGCTGTGGGAGGACAGCAGCAACGGG CAGATGTTTCACGCCCACTGGTTCTGCGCTGGGACAGACACAGTCCTCGGGGCCACGTCGGACCCTCTG GAGCTGTTCTTGGTGGATGAATGTGAGGACATGCAGCTTTCATATATCCACAGCAAAGTGAAAGTCATC TACAAAGCCCCCTCCGAAAACTGGGCCATGGAGGGAGGCATGGATCCCGAGTCCCTGCTGGAGGGGGAC GACGGGAAGACCTACTTCTACCAGCTGTGGTATGATCAAGACTACGCGAGATTCGAGTCCCCTCCAAAA ACCCAGCCAACAGAGGACAACAAGTTCAAATTCTGTGTGAGCTGTGCCCGTCTGGCTGAGATGAGGCAA AAAGAAATCCCCAGGGTCCTGGAGCAGCTCGAGGACCTGGATAGCCGGGTCCTCTACTACTCAGCCACC AAGAACGGCATCCTGTACCGAGTTGGTGATGGTGTGTACCTGCCCCCTGAGGCCTTCACGTTCAACATC AAGCTGTCCAGTCCCGTGAAACGCCCACGGAAGGAGCCCGTGGATGAGGACCTGTACCCAGAGCACTAC CGGAAATACTCCGACTACATCAAAGGCAGCAACCTGGATGCCCCTGAGCCCTACCGAATTGGCCGGATC AAAGAGATCTTCTGTCCCAAGAAGAGCAACGGCAGGCCCAATGAGACTGACATCAAAATCCGGGTCAAC AAGTTCTACAGGCCTGAGAACACCCACAAGTCCACTCCAGCGAGCTACCACGCAGACATCAACCTGCTC TACTGGAGCGACGAGGAGGCCGTGGTGGACTTCAAGGCTGTGCAGGGCCGCTGCACCGTGGAGTATGGG GAGGACCTGCCCGAGTGCGTCCAGGTGTACTCCATGGGCGGCCCCAACCGCTTCTACTTCCTCGAGGCC TATAATGCAAAGAGCAAAAGCTTTGAAGATCCTCCCAACCATGCCCGTAGCCCTGGAAACAAAGGGAAG GGCAAGGGAAAAGGGAAGGGCAAGCCCAAGTCCCAAGCCTGTGAGCCGAGCGAGCCAGAGATAGAGATC AAGCTGCCCAAGCTGCGGACCCTGGATGTGTTTTCTGGCTGCGGGGGGTTGTCGGAGGGATTCCACCAA GCAGGCATCTCTGACACGCTGTGGGCCATCGAGATGTGGGACCCTGCGGCCCAGGCGTTCCGGCTGAAC AACCCCGGCTCCACAGTGTTCACAGAGGACTGCAACATCCTGCTGAAGCTGGTCATGGCTGGGGAGACC ACCAACTCCCGCGGCCAGCGGCTGCCCCAGAAGGGAGACGTGGAGATGCTGTGCGGCGGGCCGCCCTGC CAGGGCTTCAGCGGCATGAACCGCTTCAATTCGCGCACCTACTCCAAGTTCAAAAACTCTCTGGTGGTT TCCTTCCTCAGCTACTGCGACTACTACCGGCCCCGGTTCTTCCTCCTGGAGAATGTCAGGAACTTTGTC TCCTTCAAGCGCTCCATGGTCCTGAAGCTCACCCTCCGCTGCCTGGTCCGCATGGGCTATCAGTGCACC TTCGGCGTGCTGCAGGCCGGTCAGTACGGCGTGGCCCAGACTAGGAGGCGGGCCATCATCCTGGCCGCG GCCCCTGGAGAGAAGCTCCCTCTGTTCCCGGAGCCACTGCACGTGTTTGCTCCCCGGGCCTGCCAGCTG AGCGTGGTGGTGGATGACAAGAAGTTTGTGAGCAACATAACCAGGTTGAGCTCGGGTCCTTTCCGGACC ATCACGGTGCGAGACACGATGTCCGACCTGCCGGAGGTGCGGAATGGAGCCTCGGCACTGGAGATCTCC TACAACGGGGAGCCTCAGTCCTGGTTCCAGAGGCAGCTCCGGGGCGCACAGTACCAGCCCATCCTCAGG GACCACATCTGTAAGGACATGAGTGCATTGGTGGCTGCCCGCATGCGGCACATCCCCTTGGCCCCAGGG TCAGACTGGCGCGATCTGCCCAACATCGAGGTGCGGCTCTCAGACGGCACCATGGCCAGGAAGCTGCGG TATACCCACCATGACAGGAAGAACGGCCGCAGCAGCTCTGGGGCCCTCCGTGGGGTCTGCTCCTGCGTG GAAGCCGGCAAAGCCTGCGACCCCGCAGCCAGGCAGTTCAACACCCTCATCCCCTGGTGCCTGCCCCAC ACCGGGAACCGGCACAACCACTGGGCTGGCCTCTATGGAAGGCTCGAGTGGGACGGCTTCTTCAGCACA ACCGTCACCAACCCCGAGCCCATGGGCAAGCAGGGCCGCGTGCTCCACCCAGAGCAGCACCGTGTGGTG AGCGTGCGGGAGTGTGCCCGCTCCCAGGGCTTCCCTGACACCTACCGGCTCTTCGGCAACATCCTGGAC AAGCACCGGCAGGTGGGCAATGCCGTGCCACCGCCCCTGGCCAAAGCCATTGGCTTGGAGATCAAGCTT TGTATGTTGGCCAAAGCCCGAGAGAGTGCCTCAGCTAAAATAAAGGAGGAGGAAGCTGCTAAGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC >Peptide sequence encoded by RC226414
Blue=ORF Red=Cloning site Green=Tag(s) MPARTAPARVPTLAVPAISLPDDVRRRLKDLERDSLTEKECVKEKLNLLHEFLQTEIKNQLCDLETKLR KEELSEEGYLAKVKSLLNKDLSLENGAHAYNREVNGRLENGNQARSEARRVGMADANSPPKPLSKPRTP RRSKSDGEAKRSRDPPASASQVTGIRAEPSPSPRITRKSTRQTTITSHFAKGPAKRKPQEESERAKSDE SIKEEDKDQDEKRRRVTSRERVARPLPAEEPERAKSGTRTEKEEERDEKEEKRLRSQTKEPTPKQKLKE EPDREARAGVQADEDEDGDEKDEKKHRSQPKDLAAKRRPEEKEPEKVNPQISDEKDEDEKEEKRRKTTP KEPTEKKMARAKTVMNSKTHPPKCIQCGQYLDDPDLKYGQHPPDAVDEPQMLTNEKLSIFDANESGFES YEALPQHKLTCFSVYCKHGHLCPIDTGLIEKNIELFFSGSAKPIYDDDPSLEGGVNGKNLGPINEWWIT GFDGGEKALIGFSTSFAEYILMDPSPEYAPIFGLMQEKIYISKIVVEFLQSNSDSTYEDLINKIETTVP PSGLNLNRFTEDSLLRHAQFVVEQVESYDEAGDSDEQPIFLTPCMRDLIKLAGVTLGQRRAQARRQTIR HSTREKDRGPTKATTTKLVYQIFDTFFAEQIEKDDREDKENAFKRRRCGVCEVCQQPECGKCKACKDMV KFGGSGRSKQACQERRCPNMAMKEADDDEEVDDNIPEMPSPKKMHQGKKKKQNKNRISWVGEAVKTDGK KSYYKKVCIDAETLEVGDCVSVIPDDSSKPLYLARVTALWEDSSNGQMFHAHWFCAGTDTVLGATSDPL ELFLVDECEDMQLSYIHSKVKVIYKAPSENWAMEGGMDPESLLEGDDGKTYFYQLWYDQDYARFESPPK TQPTEDNKFKFCVSCARLAEMRQKEIPRVLEQLEDLDSRVLYYSATKNGILYRVGDGVYLPPEAFTFNI KLSSPVKRPRKEPVDEDLYPEHYRKYSDYIKGSNLDAPEPYRIGRIKEIFCPKKSNGRPNETDIKIRVN KFYRPENTHKSTPASYHADINLLYWSDEEAVVDFKAVQGRCTVEYGEDLPECVQVYSMGGPNRFYFLEA YNAKSKSFEDPPNHARSPGNKGKGKGKGKGKPKSQACEPSEPEIEIKLPKLRTLDVFSGCGGLSEGFHQ AGISDTLWAIEMWDPAAQAFRLNNPGSTVFTEDCNILLKLVMAGETTNSRGQRLPQKGDVEMLCGGPPC QGFSGMNRFNSRTYSKFKNSLVVSFLSYCDYYRPRFFLLENVRNFVSFKRSMVLKLTLRCLVRMGYQCT FGVLQAGQYGVAQTRRRAIILAAAPGEKLPLFPEPLHVFAPRACQLSVVVDDKKFVSNITRLSSGPFRT ITVRDTMSDLPEVRNGASALEISYNGEPQSWFQRQLRGAQYQPILRDHICKDMSALVAARMRHIPLAPG SDWRDLPNIEVRLSDGTMARKLRYTHHDRKNGRSSSGALRGVCSCVEAGKACDPAARQFNTLIPWCLPH TGNRHNHWAGLYGRLEWDGFFSTTVTNPEPMGKQGRVLHPEQHRVVSVRECARSQGFPDTYRLFGNILD KHRQVGNAVPPPLAKAIGLEIKLCMLAKARESASAKIKEEEAAKD TRTRPLEQKLISEEDLAANDILDYKDDDDKV Recombinant protein using RC226414 also available, TP326414 |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001130823 |
| ORF Size | 4896 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq ORF | 4899 bp |
| Locus ID | 1786 |
| UniProt ID | P26358 |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Cysteine and methionine metabolism, Metabolic pathways |
| MW | 184.8 kDa |
| Gene Summary | This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016] |
Citations (5)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Structural and mechanistic insights into UHRF1-mediated DNMT1 activation in the maintenance DNA methylation
,Li, T;Wang, L;Du, Y;Xie, S;Yang, X;Lian, F;Zhou, Z;Qian, C;,
Nucleic Acids Res.
,PubMed ID 29471350
[DNMT1]
|
|
Magnetic immunoassay using CdSe/ZnS quantum dots as fluorescent probes to detect the level of DNA methyltransferase 1 in human serum sample
,Yu, F;Xiong, YM;Yu, SC;He, LL;Niu, SS;Wu, YM;Liu, J;Qu, LB;Liu, LE;Wu, YJ;,
Int J Nanomedicine
,PubMed ID 29403274
[DNMT1]
|
|
Interplay of DNA methyltransferase 1 and EZH2 through inactivation of Stat3 contributes to β-elemene-inhibited growth of nasopharyngeal carcinoma cells
,Wu, J;Tang, Q;Yang, L;Chen, Y;Zheng, F;Hann, SS;,
Sci Rep
,PubMed ID 28360411
[DNMT1]
|
|
Inhibition of EZH2 via activation of SAPK/JNK and reduction of p65 and DNMT1 as a novel mechanism in inhibition of human lung cancer cells by polyphyllin I
,Li, L;Wu, J;Zheng, F;Tang, Q;Wu, W;Hann, SS;,
J. Exp. Clin. Cancer Res.
,PubMed ID 27421653
[DNMT1]
|
|
Activation of SAPK/JNK mediated the inhibition and reciprocal interaction of DNA methyltransferase 1 and EZH2 by ursolic acid in human lung cancer cells
,Wu, J;Zhao, S;Tang, Q;Zheng, F;Chen, Y;Yang, L;Yang, X;Li, L;Wu, W;Hann, SS;,
J. Exp. Clin. Cancer Res.
,PubMed ID 26362062
[DNMT1]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226414L1 | Lenti-ORF clone of DNMT1 (Myc-DDK-tagged)-Human DNA (cytosine-5-)-methyltransferase 1 (DNMT1), transcript variant 1 |
CNY 19,152.00 |
|
| RC226414L2 | Lenti-ORF clone of DNMT1 (mGFP-tagged)-Human DNA (cytosine-5-)-methyltransferase 1 (DNMT1), transcript variant 1 |
CNY 7,600.00 |
|
| RC226414L3 | Lenti-ORF clone of DNMT1 (Myc-DDK-tagged)-Human DNA (cytosine-5-)-methyltransferase 1 (DNMT1), transcript variant 1 |
CNY 19,152.00 |
|
| RC226414L4 | Lenti-ORF clone of DNMT1 (mGFP-tagged)-Human DNA (cytosine-5-)-methyltransferase 1 (DNMT1), transcript variant 1 |
CNY 19,152.00 |
|
| RG226414 | DNMT1 (tGFP-tagged) - Human DNA (cytosine-5-)-methyltransferase 1 (DNMT1), transcript variant 1 |
CNY 18,352.00 |
|
| SC325419 | DNMT1 (untagged)-Human DNA (cytosine-5-)-methyltransferase 1 (DNMT1), transcript variant 1 |
CNY 10,450.00 |
