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DNMT1 (NM_001130823) Human Untagged Clone
CAT＃: SC325419
DNMT1 (untagged)-Human DNA (cytosine-5-)-methyltransferase 1 (DNMT1), transcript variant 1
CNY 10,450.00
| Cited in 4 publications. |
Product images
热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ADCADN; AIM; CXXC9; DNMT; HSN1E; m.HsaI; MCMT |
| Vector | PCMV6-Neo |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for NM_001130823 edited
GCGCTGCCGCCGCCGCGTCTGCTGAAGCCTCCGAGATGCCGGCGCGTACCGCCCCAGCCC GGGTGCCCACACTGGCCGTCCCGGCCATCTCGCTGCCCGACGATGTCCGCAGGCGGCTCA AAGATTTGGAAAGAGACAGCTTAACAGAAAAGGAATGTGTGAAGGAGAAATTGAATCTCT TGCACGAATTTCTGCAAACAGAAATAAAGAATCAGTTATGTGACTTGGAAACCAAATTAC GTAAAGAAGAATTATCCGAGGAGGGCTACCTGGCTAAAGTCAAATCCCTTTTAAATAAAG ATTTGTCCTTGGAGAACGGTGCTCATGCTTACAACCGGGAAGTGAATGGACGTCTAGAAA ACGGGAACCAAGCAAGAAGTGAAGCCCGTAGAGTGGGAATGGCAGATGCCAACAGCCCCC CCAAACCCCTTTCCAAACCTCGCACGCCCAGGAGGAGCAAGTCCGATGGAGAGGCTAAGC GTTCAAGAGACCCTCCTGCCTCAGCCTCCCAAGTAACTGGGATTAGAGCTGAACCTTCAC CTAGCCCCAGGATTACAAGGAAAAGCACCAGGCAAACCACCATCACATCTCATTTTGCAA AGGGCCCTGCCAAACGGAAACCTCAGGAAGAGTCTGAAAGAGCCAAATCGGATGAGTCCA TCAAGGAAGAAGACAAAGACCAGGATGAGAAGAGACGTAGAGTTACATCCAGAGAACGAG TTGCTAGACCGCTTCCTGCAGAAGAACCTGAAAGAGCAAAATCAGGAACGCGCACTGAAA AGGAAGAAGAAAGAGATGAAAAAGAAGAAAAGAGACTCCGAAGTCAAACCAAAGAACCAA CACCCAAACAGAAACTGAAGGAGGAGCCGGACAGAGAAGCCAGGGCAGGCGTGCAGGCTG ACGAGGACGAAGATGGAGACGAGAAAGATGAGAAGAAGCACAGAAGTCAACCCAAAGATC TAGCTGCCAAACGGAGGCCCGAAGAAAAAGAACCTGAAAAAGTAAATCCACAGGTTTCTG ATGAAAAAGACGAGGATGAAAAGGAGGAGAAGAGACGCAAAACGACCCCCAAAGAACCAA CGGAGAAAAAAATGGCTCGCGCCAAAACAGTCATGAACTCCAAGACCCACCCTCCCAAGT GCATTCAGTGCGGGCAGTACCTGGACGACCCTGACCTCAAATATGGGCAGCACCCACCAG ACGCGGTGGATGAGCCACAGATGCTGACAAATGAGAAGCTGTCCATCTTTGATGCCAACG AGTCTGGCTTTGAGAGTTATGAGGCGCTTCCCCAGCACAAACTGACCTGCTTCAGTGTGT ACTGTAAGCACGGTCACCTGTGTCCCATCGACACCGGCCTCATCGAGAAGAATATCGAAC TCTTCTTTTCTGGTTCAGCAAAACCAATCTATGATGATGACCCATCTCTTGAAGGTGGTG TTAATGGCAAAAATCTTGGCCCCATAAATGAATGGTGGATCACTGGCTTTGATGGAGGTG AAAAGGCCCTCATCGGCTTCAGCACCTCATTTGCCGAATACATTCTGATGGATCCCAGTC CCGAGTATGCGCCCATATTTGGGCTGATGCAGGAGAAGATCTACATCAGCAAGATTGTGG TGGAGTTCCTGCAGAGCAATTCCGACTCGACCTATGAGGACCTGATCAACAAGATCGAGA CCACGGTTCCTCCTTCTGGCCTCAACTTGAACCGCTTCACAGAGGACTCCCTCCTGCGAC ACGCGCAGTTTGTGGTGGAGCAGGTGGAGAGTTATGACGAGGCCGGGGACAGTGATGAGC AGCCCATCTTCCTGACGCCCTGCATGCGGGACCTGATCAAGCTGGCTGGGGTCACGCTGG GACAGAGGCGAGCCCAGGCGAGGCGGCAGACCATCAGGCATTCTACCAGGGAGAAGGACA GGGGACCCACGAAAGCCACCACCACCAAGCTGGTCTACCAGATCTTCGATACTTTCTTCG CAGAGCAAATTGAAAAGGATGACAGAGAAGACAAGGAGAACGCCTTTAAGCGCCGGCGAT GTGGCGTCTGTGAGGTGTGTCAGCAGCCTGAGTGTGGGAAATGTAAAGCCTGCAAGGACA TGGTTAAATTTGGTGGCAGTGGACGGAGCAAGCAGGCTTGCCAAGAGCGGAGGTGTCCCA ATATGGCCATGAAGGAGGCAGATGACGATGAGGAAGTCGATGATAACATCCCAGAGATGC CGTCACCCAAAAAAATGCACCAGGGGAAGAAGAAGAAACAGAACAAGAATCGCATCTCTT GGGTCGGAGAAGCCGTCAAGACTGATGGGAAGAAGAGTTACTATAAGAAGGTGTGCATTG ATGCGGAAACCCTGGAAGTGGGGGACTGTGTCTCTGTTATTCCAGATGATTCCTCAAAAC CGCTGTATCTAGCAAGGGTCACGGCGCTGTGGGAGGACAGCAGCAACGGGCAGATGTTTC ACGCCCACTGGTTCTGCGCTGGGACAGACACAGTCCTCGGGGCCACGTCGGACCCTCTGG AGCTGTTCTTGGTGGATGAATGTGAGGACATGCAGCTTTCATATATCCACAGCAAAGTGA AAGTCATCTACAAAGCCCCCTCCGAAAACTGGGCCATGGAGGGAGGCATGGATCCCGAGT CCCTGCTGGAGGGGGACGACGGGAAGACCTACTTCTACCAGCTGTGGTATGATCAAGACT ACGCGAGATTCGAGTCCCCTCCAAAAACCCAGCCAACAGAGGACAACAAGTTCAAATTCT GTGTGAGCTGTGCCCGTCTGGCTGAGATGAGGCAAAAAGAAATCCCCAGGGTCCTGGAGC AGCTCGAGGACCTGGATAGCCGGGTCCTCTACTACTCAGCCACCAAGAACGGCATCCTGT ACCGAGTTGGTGATGGTGTGTACCTGCCCCCTGAGGCCTTCACGTTCAACATCAAGCTGT CCAGTCCCGTGAAACGCCCACGGAAGGAGCCCGTGGATGAGGACCTGTACCCAGAGCACT ACCGGAAATACTCCGACTACATCAAAGGCAGCAACCTGGATGCCCCTGAGCCCTACCGAA TTGGCCGGATCAAAGAGATCTTCTGTCCCAAGAAGAGCAACGGCAGGCCCAATGAGACTG ACATCAAAATCCGGGTCAACAAGTTCTACAGGCCTGAGAACACCCACAAGTCCACTCCAG CGAGCTACCACGCAGACATCAACCTGCTCTACTGGAGCGACGAGGAGGCCGTGGTGGACT TCAAGGCTGTGCAGGGCCGCTGCACCGTGGAGTATGGGGAGGACCTGCCCGAGTGCGTCC AGGTGTACTCCATGGGCGGCCCCAACCGCTTCTACTTCCTCGAGGCCTATAATGCAAAGA GCAAAAGCTTTGAAGATCCTCCCAACCATGCCCGTAGCCCTGGAAACAAAGGGAAGGGCA AGGGAAAAGGGAAGGGCAAGCCCAAGTCCCAAGCCTGTGAGCCGAGCGAGCCAGAGATAG AGATCAAGCTGCCCAAGCTGCGGACCCTGGATGTGTTTTCTGGCTGCGGGGGGTTGTCGG AGGGATTCCACCAAGCAGGCATCTCTGACACGCTGTGGGCCATCGAGATGTGGGACCCTG CGGCCCAGGCGTTCCGGCTGAACAACCCCGGCTCCACAGTGTTCACAGAGGACTGCAACA TCCTGCTGAAGCTGGTCATGGCTGGGGAGACCACCAACTCCCGCGGCCAGCGGCTGCCCC AGAAGGGAGACGTGGAGATGCTGTGCGGCGGGCCGCCCTGCCAGGGCTTCAGCGGCATGA ACCGCTTCAATTCGCGCACCTACTCCAAGTTCAAAAACTCTCTGGTGGTTTCCTTCCTCA GCTACTGCGACTACTACCGGCCCCGGTTCTTCCTCCTGGAGAATGTCAGGAACTTTGTCT CCTTCAAGCGCTCCATGGTCCTGAAGCTCACCCTCCGCTGCCTGGTCCGCATGGGCTATC AGTGCACCTTCGGCGTGCTGCAGGCCGGTCAGTACGGCGTGGCCCAGACTAGGAGGCGGG CCATCATCCTGGCCGCGGCCCCTGGAGAGAAGCTCCCTCTGTTCCCGGAGCCACTGCACG TGTTTGCTCCCCGGGCCTGCCAGCTGAGCGTGGTGGTGGATGACAAGAAGTTTGTGAGCA ACATAACCAGGTTGAGCTCGGGTCCTTTCCGGACCATCACGGTGCGAGACACGATGTCCG ACCTGCCGGAGGTGCGGAATGGAGCCTCGGCACTGGAGATCTCCTACAACGGGGAGCCTC AGTCCTGGTTCCAGAGGCAGCTCCGGGGCGCACAGTACCAGCCCATCCTCAGGGACCACA TCTGTAAGGACATGAGTGCATTGGTGGCTGCCCGCATGCGGCACATCCCCTTGGCCCCAG GGTCAGACTGGCGCGATCTGCCCAACATCGAGGTGCGGCTCTCAGACGGCACCATGGCCA GGAAGCTGCGGTATACCCACCATGACAGGAAGAACGGCCGCAGCAGCTCTGGGGCCCTCC GTGGGGTCTGCTCCTGCGTGGAAGCCGGCAAAGCCTGCGACCCCGCAGCCAGGCAGTTCA ACACCCTCATCCCCTGGTGCCTGCCCCACACCGGGAACCGGCACAACCACTGGGCTGGCC TCTATGGAAGGCTCGAGTGGGACGGCTTCTTCAGCACAACCGTCACCAACCCCGAGCCCA TGGGCAAGCAGGGCCGCGTGCTCCACCCAGAGCAGCACCGTGTGGTGAGCGTGCGGGAGT GTGCCCGCTCCCAGGGCTTCCCTGACACCTACCGGCTCTTCGGCAACATCCTGGACAAGC ACCGGCAGGTGGGCAATGCCGTGCCACCGCCCCTGGCCAAAGCCATTGGCTTGGAGATCA AGCTTTGTATGTTGGCCAAAGCCCGAGAGAGTGCCTCAGCTAAAATAAAGGAGGAGGAAG CTGCTAAGGACTAGTTCTGCCCTCCCGTCACCCCTGTTTCTGGCACCAGGAATCCCCAAC ATGCACTGATGTTGTGTTTTTAACATGTCAATCTGTCCGTTCACATGTGTGGTACATGGT GTTTGTGGCCTTGGCTGACATGAAGCTGTTGTGTGAGGTTCGCTTATCAACTAATGATTT AGTGATCAAATTGTGCAGTACTTTGTGCATTCTGGATTTTAAAAGTTTTTTATTATGCAT TATATCAAATCTACCACTGTATGAGTGGAAATTAAGACTTTATGTAGTTTTTATATGTTG TAATATTTCTTCAAATAAATCTCTCCTATAAACCAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_001130823 |
| Insert Size | 5300 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | The ORF of this clone has been fully sequenced and found to contain one SNP compared with NM_001130823.1. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001130823.1, NP_001124295.1 |
| RefSeq Size | 5425 bp |
| RefSeq ORF | 4899 bp |
| Locus ID | 1786 |
| UniProt ID | P26358 |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Cysteine and methionine metabolism, Metabolic pathways |
| Gene Summary | This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016] Transcript Variant: This variant (1, also known as Dnmt1b in PMIDs 10449766 and 10753866) represents the longest transcript and encodes the longest isoform (a). |
Citations (4)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Decreased KAT5 Expression Impairs DNA Repair and Induces Altered DNA Methylation in Kidney Podocytes
,Hishikawa, A;Hayashi, K;Abe, T;Kaneko, M;Yokoi, H;Azegami, T;Nakamura, M;Yoshimoto, N;Kanda, T;Sakamaki, Y;Itoh, H;,
Cell Rep
,PubMed ID 30699357
[DNMT1]
|
|
RFTS-deleted DNMT1 enhances tumorigenicity with focal hypermethylation and global hypomethylation
,null,
Cell Cycle
,PubMed ID 25485502
[DNMT1]
|
|
Intrinsic and extrinsic regulation of DNA methylation during malignant transformation
,Wu, BK;,
Thesis
[DNMT1]
|
|
Toll-like receptor 3 (TLR3) activation induces microRNA-dependent reexpression of functional RARß and tumor regression
,Roberta Galli, Alessio Paone, Muller Fabbri, Nicola Zanesi, Federica Calore, Luciano Cascione, Mario Acunzo, Antonella Stoppacciaro, Andrea Tubaro, Francesca Lovat, Pierluigi Gasparini, Paolo Fadda, Hansjuerg Alder, Stefano Volinia, Antonio Filippini, Elio Ziparo, Anna Riccioli, and Carlo M. Croce,
PNAS, Jun 2013; 110: 9812 - 9817.
,PubMed ID 23716670
[DNMT1]
|
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| RC226414L4 | Lenti-ORF clone of DNMT1 (mGFP-tagged)-Human DNA (cytosine-5-)-methyltransferase 1 (DNMT1), transcript variant 1 |
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