MYO9B (NM_001130065) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CELIAC4; MYR5
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC226439 representing NM_001130065 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGTGAAAGAGGCAGGCAGCTCGGGCCGCCGGGAGCAGGCGGCCTACCACCTGCACATCTACCCCC AGCTGTCCACCACCGAGAGCCAGGCCTCGTGCCGCGTGACTGCCACCAAGGACAGCACCACCTCGGACGT CATCAAGGACGCCATTGCCAGCCTGCGGCTGGACGGCACCAAATGTTATGTGCTGGTGGAGGTCAAAGAG TCGGGAGGCGAGGAATGGGTGCTGGACGCCAACGACTCGCCTGTGCACCGGGTGCTGCTATGGCCCCGGC GGGCACAGGACGAGCACCCTCAGGAGGATGGCTACTACTTCCTGCTGCAGGAGCGCAACGCAGATGGAAC CATCAAGTACGTGCATATGCAGCTGGTGGCGCAGGCCACAGCCACCCGGCGCCTAGTGGAGCGTGGCCTC CTGCCACGGCAGCAGGCGGACTTTGATGACCTGTGTAACCTCCCCGAGCTAACCGAGGGCAACCTCCTGA AGAACCTCAAGCACCGCTTCCTGCAACAAAAGATCTACACGTACGCGGGGAGCATCCTGGTGGCCATCAA CCCCTTTAAGTTCCTGCCCATCTACAACCCCAAGTACGTGAAGATGTATGAGAACCAGCAGCTGGGCAAG CTGGAGCCACACGTCTTCGCGCTGGCCGACGTGGCCTACTACACCATGCTCAGGAAGCGCGTGAACCAGT GCATCGTGATCTCGGGTGAGAGCGGCTCCGGCAAGACCCAGAGCACCAACTTCCTCATCCACTGCCTCAC CGCCCTCAGCCAGAAGGGCTACGCCAGCGGCGTCGAGAGGACCATCCTGGGTGCTGGCCCTGTGCTGGAG GCTTTTGGAAATGCCAAGACAGCCCACAACAACAACTCCAGCCGGTTTGGGAAATTCATCCAAGTCAGCT ACCTAGAGAGTGGCATCGTGAGAGGAGCTGTCGTCGAGAAATATCTGCTTGAAAAGTCTCGCCTGGTGTC TCAGGAGAAGGATGAGAGGAACTACCATGTGTTTTATTATTTGTTACTTGGGGTCAGCGAGGAAGAGCGC CAAGAATTTCAGCTCAAGCAGCCTGAAGATTATTTCTACCTCAACCAGCATAACTTGAAGATTGAAGATG GGGAGGACCTGAAGCATGACTTTGAGAGGCTCAAGCAGGCCATGGAGATGGTGGGCTTCCTCCCCGCCAC CAAGAAGCAGATTTTTGCCGTCCTCTCGGCCATCCTGTACCTGGGCAACGTCACTTATAAGAAGAGAGCT ACAGGCCGAGAGGAAGGGTTGGAGGTCGGGCCACCCGAGGTGCTGGACACCCTGTCGCAGCTTCTGAAGG TGAAGCGAGAAATCTTGGTGGAGGTTCTGACCAAAAGAAAAACGGTGACCGTCAACGACAAGCTTATCCT TCCCTACAGCCTCAGCGAGGCCATCACTGCCCGCGACTCCATGGCCAAGTCTCTGTACAGCGCCCTGTTC GACTGGATTGTGCTGCGGATCAACCACGCACTCCTCAACAAGAAGGACGTGGAAGAGGCAGTCTCGTGCC TGTCCATTGGGGTCCTGGACATCTTCGGGTTTGAAGACTTCGAGAGGAACAGCTTTGAGCAGTTCTGCAT CAACTACGCCAATGAGCAGCTGCAGTATTACTTCAACCAGCACATCTTCAAGCTGGAGCAGGAGGAATAT CAGGGCGAGGGGATCACGTGGCACAACATCGGCTACACAGACAATGTCGGCTGCATCCATCTCATCAGCA AGAAACCCACGGGCCTCTTCTACCTGCTGGACGAGGAGAGCAACTTCCCCCACGCCACGAGCCAGACCCT GCTGGCCAAGTTCAAACAGCAACATGAGGACAATAAGTACTTCCTGGGCACCCCGGTCATGGAGCCAGCT TTCATCATCCAGCACTTCGCAGGGAAGGTGAAATATCAGATCAAGGACTTCCGGGAGAAGAACATGGACT ACATGCGGCCAGACATCGTGGCCCTGCTGCGGGGCAGTGACAGCTCCTACGTGCGGGAGCTCATCGGCAT GGACCCCGTGGCCGTGTTCCGCTGGGCCGTGCTCCGGGCTGCTATCCGGGCCATGGCAGTGCTTCGGGAG GCCGGACGCCTGCGGGCCGAGAGGGCCGAAAAGGCTGCAGGTATGAGCAGCCCTGGTGCCCAAAGTCACC CAGAAGAGCTGCCAAGAGGAGCCAGCACCCCTTCGGAAAAACTTTACCGCGATTTGCATAACCAAATGAT CAAGAGCATCAAAGGATTGCCCTGGCAGGGCGAGGACCCCCGTAGCCTTCTCCAGTCCCTCAGTCGGCTC CAGAAACCCCGCGCCTTCATCCTGAAAAGTAAAGGTATCAAACAAAAGCAGATCATTCCAAAGAACCTAC TGGACTCCAAGTCCCTGAAACTCATCATCAGCATGACTCTGCACGACCGCACCACCAAGTCCCTACTGCA CCTGCACAAGAAGAAAAAGCCACCAAGCATCAGCGCCCAGTTCCAGACATCCCTTAACAAGCTCTTGGAG GCACTGGGGAAGGCGGAGCCCTTCTTTATCCGCTGCATCCGTTCCAATGCTGAAAAGAAAGAGCTGTGCT TTGACGACGAGCTGGTCCTGCAGCAGCTGCGCTACACCGGCATGCTGGAGACCGTGCGCATCCGGAGGTC AGGGTACAGCGCCAAGTACACGTTCCAGGATTTCACCGAGCAGTTCCAGGTGCTCCTGCCCAAGGATGCC CAGCCCTGCAGGGAGGTCATCTCCACCCTCCTGGAGAAAATGAAGATAGACAAGAGGAACTACCAGATCG GGAAGACCAAGGTCTTCCTGAAGGAGACGGAGCGGCAAGCCCTGCAGGAGACGCTGCACCGGGAGGTGGT GCGGAAAATCCTGCTGCTGCAGAGCTGGTTCCGGATGGTGCTGGAGCGTCGGCACTTCCTGCAGATGAAG CGGGCCGCCGTCACCATCCAGGCCTGCTGGCGGTCCTACCGGGTCCGGAGGGCGCTGGAGAGGACGCAGG CTGCCGTGTACCTCCAGGCCTCATGGAGGGGCTACTGGCAGCGGAAGCTCTACCGGCACCAGAAACAGAG CATCATCCGCCTGCAGAGCCTGTGTCGGGGGCACCTGCAGCGCAAGAGCTTCAGCCAGATGATCTCGGAG AAGCAGAAGGCAGAAGAGAAGGAGAGGGAAGCCCTGGAAGCCGCAAGAGCAGGTGCTGAGGAGGGCGGAC AGGGTCAGGCGGCTGGAGGGCAGCAGGTAGCTGAGCAGGGGCCGGAGCCAGCGGAGGATGGCGGGCACCT GGCATCGGAGCCTGAGGTGCAGCCAAGTGACAGGTCCCCCCTAGAGCACTCCTCACCTGAGAAGGAGGCC CCAAGCCCAGAGAAGACTCTCCCACCCCAGAAAACCGTGGCGGCTGAAAGTCACGAGAAAGTCCCCAGCA GCCGGGAGAAGCGTGAGTCGCGTCGGCAAAGAGGGCTGGAGCACGTCAAGTTCCAGAACAAACACATCCA GTCCTGCAAGGAGGAGAGTGCCCTCAGAGAACCTTCCAGAAGGGTCACCCAGGAGCAAGGGGTGAGTCTC CTGGAAGACAAAAAGGAGAGCAGAGAAGATGAAACCCTTCTAGTCGTAGAGACGGAGGCTGAGAACACAT CTCAAAAGCAGCCCACAGAGCAACCCCAGGCCATGGCAGTTGGCAAGGTCTCTGAAGAAACTGAGAAGAC GCTGCCCAGTGGGAGCCCCAGGCCTGGCCAGTTGGAGCGGCCGACCAGCCTGGCCCTGGACAGCAGGGTC AGCCCACCGGCCCCTGGCAGCGCCCCCGAGACCCCCGAGGACAAGAGCAAACCATGTGGCAGCCCAAGGG TTCAGGAAAAGCCCGACAGCCCCGGAGGCTCCACGCAGATCCAGCGGTACCTGGACGCCGAGCGGCTGGC CAGCGCCGTGGAACTGTGGCGGGGCAAGAAGCTGGTGGCCGCCGCCAGCCCTAGTGCCATGCTCAGCCAG TCCCTGGACCTCAGCGACAGACACCGGGCCACAGGGGCCGCCCTCACGCCCACAGAGGAGAGGCGCACCT CCTTCTCCACGAGCGACGTCTCCAAGCTCCTCCCGTCCCTGGCCAAGGCTCAGCCTGCAGCAGAAACCAC GGACGGAGAGCGAAGTGCGAAAAAGCCAGCTGTCCAGAAGAAGAAGCCAGGCGACGCATCCTCCCTCCCA GACGCAGGGCTGTCCCCGGGCTCTCAGGTCGACTCTAAATCCACGTTTAAGAGGCTTTTTCTGCATAAAA CCAAGGATAAAAAATACAGCCTGGAGGGCGCAGAGGAGCTGGAGAATGCAGTGTCCGGGCACGTGGTGCT GGAAGCCACCACCATGAAGAAGGGCCTGGAAGCCCCCTCCGGACAGCAGCATCGCCACGCTGCAGGTGAG AAGCGCACCAAGGAACCAGGAGGCAAAGGGAAGAAGAACCGAAATGTCAAGATTGGGAAGATCACAGTGT CAGAGAAGTGGCGGGAATCGGTGTTCCGCCAGATCACCAACGCCAATGAGCTCAAGTACCTGGACGAGTT CCTGCTCAACAAGATAAATGACCTCCGTTCCCAGAAGACGCCCATTGAGAGCTTGTTTATCGAAGCCACC GAGAAGTTCAGGAGCAACATCAAAACGATGTACTCTGTCCCGAACGGGAAGATCCACGTGGGCTACAAGG ATCTGATGGAGAACTACCAGATCGTCGTCAGCAACCTGGCCACTGAGCGTGGCCAGAAGGACACCAACCT GGTCCTCAACCTCTTCCAGTCACTGCTAGATGAGTTCACCCGTGGCTACACCAAGAACGACTTCGAGCCA GTGAAGCAGAGCAAAGCTCAGAAGAAGAAGCGGAAGCAGGAGCGTGCTGTCCAGGAGCACAACGGGCACG TGTTCGCCAGCTACCAGGTTAGCATCCCGCAGTCGTGCGAGCAGTGCCTCTCCTATATCTGGCTCATGGA CAAGGCCCTGCTCTGCAGCGTGTGCAAGATGACCTGCCACAAGAAGTGCGTGCACAAGATTCAGAGCCAC TGCTCCTACACCTACGGGAGGAAGGGCGAGCCAGGCGTTGAGCCTGGCCACTTCGGCGTGTGCGTAGACA GCCTGACCAGCGACAAGGCCTCGGTGCCCATCGTGCTGGAGAAGCTCCTGGAACACGTGGAGATGCACGG CCTGTACACCGAGGGCCTCTACCGCAAGTCGGGTGCTGCCAACCGCACTCGGGAGCTCCGGCAGGCGCTG CAGACAGACCCCGCAGCAGTCAAGCTGGAGAACTTCCCCATCCACGCCATCACAGGGGTGCTGAAGCAGT GGCTGCGGGAGCTGCCCGAGCCCCTCATGACCTTCGCACAGTACGGCGACTTCCTCCGAGCCGTCGAGCT GCCGGAGAAGCAGGAGCAGCTGGCTGCCATCTATGCCGTCCTGGAGCACCTTCCAGAAGCCAACCACAAC TCCCTGGAGAGACTCATCTTCCACCTTGTCAAGGTGGCCCTGCTCGAGGATGTCAACCGCATGTCACCTG GGGCGCTGGCCATTATCTTCGCACCCTGCCTCCTGCGCTGCCCTGACAACTCGGACCCGCTGACCAGCAT GAAGGACGTCCTCAAGATCACCACGTGCGTGGAGATGCTGATCAAGGAGCAGATGAGGAAATACAAAGTG AAGATGGAGGAGATCAGCCAACTGGAGGCTGCAGAGAGTATCGCCTTCCGCAGGCTTTCGCTCCTGCGAC AAAATGCTCCATGGCCTCTCAAACTGGGGTTTTCGTCTCCCTATGAGGGGGTCCTGAACAAGAGCCCCAA GACCCGGGACATCCAGGAGGAGGAGCTGGAGGTGCTGCTGGAGGAGGAGGCAGCCGGCGGCGATGAGGAC CGGGAAAAGGAGATTCTCATTGAACGGATCCAGTCCATCAAGGAGGAGAAGGAGGACATCACCTACCGGC TGCCGGAGCTGGACCCAAGGGGCTCGGACGAGGAGAACCTGGACTCGGAGACGTCGGCCAGCACCGAGAG CCTGCTGGAGGAGCGGGCCGGGCGGGGGGCCTCGGAAGGTCAGTAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC226439 representing NM_001130065 Red=Cloning site Green=Tags(s) MSVKEAGSSGRREQAAYHLHIYPQLSTTESQASCRVTATKDSTTSDVIKDAIASLRLDGTKCYVLVEVKE SGGEEWVLDANDSPVHRVLLWPRRAQDEHPQEDGYYFLLQERNADGTIKYVHMQLVAQATATRRLVERGL LPRQQADFDDLCNLPELTEGNLLKNLKHRFLQQKIYTYAGSILVAINPFKFLPIYNPKYVKMYENQQLGK LEPHVFALADVAYYTMLRKRVNQCIVISGESGSGKTQSTNFLIHCLTALSQKGYASGVERTILGAGPVLE AFGNAKTAHNNNSSRFGKFIQVSYLESGIVRGAVVEKYLLEKSRLVSQEKDERNYHVFYYLLLGVSEEER QEFQLKQPEDYFYLNQHNLKIEDGEDLKHDFERLKQAMEMVGFLPATKKQIFAVLSAILYLGNVTYKKRA TGREEGLEVGPPEVLDTLSQLLKVKREILVEVLTKRKTVTVNDKLILPYSLSEAITARDSMAKSLYSALF DWIVLRINHALLNKKDVEEAVSCLSIGVLDIFGFEDFERNSFEQFCINYANEQLQYYFNQHIFKLEQEEY QGEGITWHNIGYTDNVGCIHLISKKPTGLFYLLDEESNFPHATSQTLLAKFKQQHEDNKYFLGTPVMEPA FIIQHFAGKVKYQIKDFREKNMDYMRPDIVALLRGSDSSYVRELIGMDPVAVFRWAVLRAAIRAMAVLRE AGRLRAERAEKAAGMSSPGAQSHPEELPRGASTPSEKLYRDLHNQMIKSIKGLPWQGEDPRSLLQSLSRL QKPRAFILKSKGIKQKQIIPKNLLDSKSLKLIISMTLHDRTTKSLLHLHKKKKPPSISAQFQTSLNKLLE ALGKAEPFFIRCIRSNAEKKELCFDDELVLQQLRYTGMLETVRIRRSGYSAKYTFQDFTEQFQVLLPKDA QPCREVISTLLEKMKIDKRNYQIGKTKVFLKETERQALQETLHREVVRKILLLQSWFRMVLERRHFLQMK RAAVTIQACWRSYRVRRALERTQAAVYLQASWRGYWQRKLYRHQKQSIIRLQSLCRGHLQRKSFSQMISE KQKAEEKEREALEAARAGAEEGGQGQAAGGQQVAEQGPEPAEDGGHLASEPEVQPSDRSPLEHSSPEKEA PSPEKTLPPQKTVAAESHEKVPSSREKRESRRQRGLEHVKFQNKHIQSCKEESALREPSRRVTQEQGVSL LEDKKESREDETLLVVETEAENTSQKQPTEQPQAMAVGKVSEETEKTLPSGSPRPGQLERPTSLALDSRV SPPAPGSAPETPEDKSKPCGSPRVQEKPDSPGGSTQIQRYLDAERLASAVELWRGKKLVAAASPSAMLSQ SLDLSDRHRATGAALTPTEERRTSFSTSDVSKLLPSLAKAQPAAETTDGERSAKKPAVQKKKPGDASSLP DAGLSPGSQVDSKSTFKRLFLHKTKDKKYSLEGAEELENAVSGHVVLEATTMKKGLEAPSGQQHRHAAGE KRTKEPGGKGKKNRNVKIGKITVSEKWRESVFRQITNANELKYLDEFLLNKINDLRSQKTPIESLFIEAT EKFRSNIKTMYSVPNGKIHVGYKDLMENYQIVVSNLATERGQKDTNLVLNLFQSLLDEFTRGYTKNDFEP VKQSKAQKKKRKQERAVQEHNGHVFASYQVSIPQSCEQCLSYIWLMDKALLCSVCKMTCHKKCVHKIQSH CSYTYGRKGEPGVEPGHFGVCVDSLTSDKASVPIVLEKLLEHVEMHGLYTEGLYRKSGAANRTRELRQAL QTDPAAVKLENFPIHAITGVLKQWLRELPEPLMTFAQYGDFLRAVELPEKQEQLAAIYAVLEHLPEANHN SLERLIFHLVKVALLEDVNRMSPGALAIIFAPCLLRCPDNSDPLTSMKDVLKITTCVEMLIKEQMRKYKV KMEEISQLEAAESIAFRRLSLLRQNAPWPLKLGFSSPYEGVLNKSPKTRDIQEEELEVLLEEEAAGGDED REKEILIERIQSIKEEKEDITYRLPELDPRGSDEENLDSETSASTESLLEERAGRGASEGQY TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001130065
ORF Size	6066 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001130065.2
RefSeq ORF	6069 bp
Locus ID	4650
MW	229 kDa
Gene Summary	This gene encodes a member of the myosin family of actin-based molecular motor heavy chain proteins. The protein represents an unconventional myosin; it should not be confused with the conventional non-muscle myosin-9 (MYH9). The protein has four IQ motifs located in the neck domain that bind calmodulin, which serves as a light chain. The protein complex has a single-headed structure and exhibits processive movement on actin filaments toward the minus-end. The protein also has rho-GTPase activity. Polymorphisms in this gene are associated with celiac disease and ulcerative colitis susceptibility. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

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