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GRAF (ARHGAP26) (NM_001135608) Human Tagged ORF Clone
CAT＃: RC226597
ARHGAP26 (Myc-DDK-tagged)-Human Rho GTPase activating protein 26 (ARHGAP26), transcript variant 2
ORF Plasmid: tGFP
"NM_001135608" in other vectors (6)
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CNY 5,560.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GRAF; GRAF1; OPHN1L; OPHN1L1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC226597 representing NM_001135608
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCTCCCAGCGCTCGAGTTCAGCGACTGCTGCCTCGATAGTCCGCACTTCCGAGAGACGCTCAAGT CGCACGAAGCAGAGCTGGACAAGACCAACAAATTCATCAAGGAGCTCATCAAGGACGGGAAGTCACTCAT AAGCGCGCTCAAGAATTTGTCTTCAGCGAAGCGGAAGTTTGCAGATTCCTTAAATGAATTTAAATTTCAG TGCATAGGAGATGCAGAAACAGATGATGAGATGTGTATAGCAAGGTCTTTGCAGGAGTTTGCCACTGTCC TCAGGAATCTTGAAGATGAACGGATACGGATGATTGAGAATGCCAGCGAGGTGCTCATCACTCCCTTGGA GAAGTTTCGAAAGGAACAGATCGGGGCTGCCAAGGAAGCCAAAAAGAAGTATGACAAAGAGACAGAAAAG TATTGTGGCATCTTAGAAAAACACTTGAATTTGTCTTCCAAAAAGAAAGAATCTCAGCTTCAGGAGGCAG ACAGCCAAGTGGACCTGGTCCGGCAGCATTTCTATGAAGTATCCCTGGAATATGTCTTCAAGGTGCAGGA AGTCCAAGAGAGAAAGATGTTTGAGTTTGTGGAGCCTCTGCTGGCCTTCCTGCAAGGACTCTTCACTTTC TATCACCATGGTTACGAACTGGCCAAGGATTTCGGGGACTTCAAGACACAGTTAACCATTAGCATACAGA ACACAAGAAATCGCTTTGAAGGCACTAGATCAGAAGTGGAATCACTGATGAAAAAGATGAAGGAGAATCC CCTTGAGCACAAGACCATCAGTCCCTACACCATGGAGGGATACCTCTACGTGCAGGAGAAACGTCACTTT GGAACTTCTTGGGTGAAGCACTACTGTACATATCAACGGGATTCCAAACAAATCACCATGGTACCATTTG ACCAAAAGTCAGGAGGAAAAGGGGGAGAAGATGAATCAGTTATCCTCAAATCCTGCACACGGCGGAAAAC AGACTCCATTGAGAAGAGGTTTTGCTTTGATGTGGAAGCAGTAGACAGGCCAGGGGTTATCACCATGCAA GCTTTGTCGGAAGAGGACCGGAGGCTCTGGATGGAAGCCATGGATGGCCGGGAACCTGTCTACAACTCGA ACAAAGACAGCCAGAGTGAAGGGACTGCGCAGTTGGACAGCATTGGCTTCAGCATAATCAGGAAATGCAT CCATGCTGTGGAAACCAGAGGGATCAACGAGCAAGGGCTGTATCGAATTGTGGGGGTCAACTCCAGAGTG CAGAAGTTGCTGAGTGTCCTGATGGACCCCAAGACTGCTTCTGAGACAGAAACAGATATCTGTGCTGAAT GGGAGATAAAGACCATCACTAGTGCTCTGAAGACCTACCTAAGAATGCTTCCAGGACCACTCATGATGTA CCAGTTTCAAAGAAGTTTCATCAAAGCAGCAAAACTGGAGAACCAGGAGTCTCGGGTCTCTGAAATCCAC AGCCTTGTTCATCGGCTCCCAGAGAAAAATCGGCAGATGTTACAGCTGCTCATGAACCACTTGGCAAATG TTGCTAACAACCACAAGCAGAATTTGATGACGGTGGCAAACCTTGGTGTGGTGTTTGGACCCACTCTGCT GAGGCCTCAGGAAGAAACAGTAGCAGCCATCATGGACATCAAATTTCAGAACATTGTCATTGAGATCCTA ATAGAAAACCACGAAAAGATATTTAACACCGTGCCCGATATGCCTCTCACCAATGCCCAGCTGCACCTGT CTCGGAAGAAGAGCAGTGACTCCAAGCCCCCGTCCTGCAGCGAGAGGCCCCTGACGCTCTTCCACACCGT TCAGTCAACAGAGAAACAGGAACAAAGGAACAGCATCATCAACTCCAGTTTGGAATCTGTCTCATCAAAT CCAAACAGCATCCTTAATTCCAGCAGCAGCTTACAGCCCAACATGAACTCCAGTGACCCAGACCTGGCTG TGGTCAAACCCACCCGGCCCAACTCACTCCCCCCGAATCCAAGCCCAACTTCACCCCTCTCGCCATCTTG GCCCATGTTCTCGGCGCCATCCAGCCCTATGCCCACCTCATCCACGTCCAGCGACTCATCCCCCGTCAGC ACACCGTTCCGGAAGGCAAAAGCCTTGTATGCCTGCAAAGCTGAACATGACTCAGAACTTTCGTTCACAG CAGGCACGGTCTTCGATAATGTTCACCCATCTCAGGAGCCTGGCTGGTTGGAGGGGACTCTGAACGGAAA GACTGGCCTCATCCCTGAGAATTACGTGGAGTTCCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC226597 representing NM_001135608
Red=Cloning site Green=Tags(s) MGLPALEFSDCCLDSPHFRETLKSHEAELDKTNKFIKELIKDGKSLISALKNLSSAKRKFADSLNEFKFQ CIGDAETDDEMCIARSLQEFATVLRNLEDERIRMIENASEVLITPLEKFRKEQIGAAKEAKKKYDKETEK YCGILEKHLNLSSKKKESQLQEADSQVDLVRQHFYEVSLEYVFKVQEVQERKMFEFVEPLLAFLQGLFTF YHHGYELAKDFGDFKTQLTISIQNTRNRFEGTRSEVESLMKKMKENPLEHKTISPYTMEGYLYVQEKRHF GTSWVKHYCTYQRDSKQITMVPFDQKSGGKGGEDESVILKSCTRRKTDSIEKRFCFDVEAVDRPGVITMQ ALSEEDRRLWMEAMDGREPVYNSNKDSQSEGTAQLDSIGFSIIRKCIHAVETRGINEQGLYRIVGVNSRV QKLLSVLMDPKTASETETDICAEWEIKTITSALKTYLRMLPGPLMMYQFQRSFIKAAKLENQESRVSEIH SLVHRLPEKNRQMLQLLMNHLANVANNHKQNLMTVANLGVVFGPTLLRPQEETVAAIMDIKFQNIVIEIL IENHEKIFNTVPDMPLTNAQLHLSRKKSSDSKPPSCSERPLTLFHTVQSTEKQEQRNSIINSSLESVSSN PNSILNSSSSLQPNMNSSDPDLAVVKPTRPNSLPPNPSPTSPLSPSWPMFSAPSSPMPTSSTSSDSSPVS TPFRKAKALYACKAEHDSELSFTAGTVFDNVHPSQEPGWLEGTLNGKTGLIPENYVEFL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001135608 |
| ORF Size | 2277 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001135608.3 |
| RefSeq ORF | 2280 bp |
| Locus ID | 23092 |
| UniProt ID | Q9UNA1 |
| Protein Families | Druggable Genome |
| MW | 86 kDa |
| Gene Summary | Interaction of a cell with the extracellular matrix triggers integrin cell surface receptors to begin signaling cascades that regulate the organization of the actin-cytoskeleton. One of the proteins involved in these cascades is focal adhesion kinase. The protein encoded by this gene is a GTPase activating protein that binds to focal adhesion kinase and mediates the activity of the GTP binding proteins RhoA and Cdc42. Defects in this gene are a cause of juvenile myelomonocytic leukemia (JMML). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226597L1 | Lenti-ORF clone of ARHGAP26 (Myc-DDK-tagged)-Human Rho GTPase activating protein 26 (ARHGAP26), transcript variant 2 |
CNY 7,960.00 |
|
| RC226597L2 | Lenti-ORF clone of ARHGAP26 (mGFP-tagged)-Human Rho GTPase activating protein 26 (ARHGAP26), transcript variant 2 |
CNY 7,700.00 |
|
| RC226597L3 | Lenti-ORF clone of ARHGAP26 (Myc-DDK-tagged)-Human Rho GTPase activating protein 26 (ARHGAP26), transcript variant 2 |
CNY 7,700.00 |
|
| RC226597L4 | Lenti-ORF clone of ARHGAP26 (mGFP-tagged)-Human Rho GTPase activating protein 26 (ARHGAP26), transcript variant 2 |
CNY 7,700.00 |
|
| RG226597 | ARHGAP26 (tGFP-tagged) - Human Rho GTPase activating protein 26 (ARHGAP26), transcript variant 2 |
CNY 7,160.00 |
|
| SC325319 | ARHGAP26 (untagged)-Human Rho GTPase activating protein 26 (ARHGAP26), transcript variant 2 |
CNY 7,220.00 |
