PCDH15 (NM_001142765) Human Tagged ORF Clone
CAT#: RC226598
- TrueORF®
PCDH15 (Myc-DDK-tagged)-Human protocadherin-related 15 (PCDH15), transcript variant D
ORF Plasmid: tGFP
"NM_001142765" in other vectors (2)
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CNY 18,810.00
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | CDHR15; DFNB23; USH1F |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC226598 representing NM_001142765
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTCGACAGTTTTATCTCTGGACATGTTTAGCTTCAGGGATCATCCTGGGCTCTCTCTTTGAAATCT GCTTGGGCCAGTATGATGATGATTGCAAACTAGCTAGGGGAGGACCACCAGCTACCATAGTTGCTATTGA TGAAGAAAGTCGGAATGGTACAATTCTGGTGGACAACATGCTGATCAAAGGGACTGCTGGAGGACCAGAC CCCACCATAGAACTTTCTTTAAAGGATAATGTGGATTACTGGGTGTTGATGGATCCTGTTAAGCAAATGC TTTTCCTGAACAGCACCGGAAGAGTTCTGGATAGAGATCCACCGATGAACATACACTCCATTGTGGTGCA GGTCCAGTGCATCAACAAAAAAGTGGGCACTATTATCTACCATGAAGTGCGAATAGTGGTGAGAGACAGG AATGACAACTCACCCACTTTCAAGCATGAAAGCTACTATGCCACAGTGAATGAGCTCACTCCAGTTGGTA CCACAATATTCACAGGATTTTCAGGAGACAATGGAGCTACAGATATAGATGATGGACCAAATGGACAGAT AGAGTATGTTATTCAGTATAATCCAGATGATCCGACATCCAATGACACCTTTGAAATTCCCCTAATGTTG ACTGGAAATATAGTGTTAAGGAAGAGGCTCAACTATGAAGATAAGACTCGCTACTTTGTCATAATCCAAG CTAATGACCGTGCCCAAAATCTGAATGAGAGGCGAACCACCACCACCACTCTCACAGTGGATGTTCTGGA TGGAGATGACTTGGGTCCAATGTTTCTTCCTTGTGTCCTTGTGCCAAACACTCGTGATTGCCGTCCACTC ACTTATCAAGCTGCCATACCTGAGTTGAGAACTCCGGAAGAACTGAACCCCATTATTGTTACGCCACCAA TCCAAGCCATTGATCAGGACCGGAATATTCAACCGCCATCAGATAGGCCAGGAATCCTCTATTCCATCCT TGTTGGGACTCCTGAGGATTACCCACGATTTTTCCATATGCATCCTAGGACAGCAGAACTTAGTCTCCTG GAGCCAGTAAACAGAGACTTTCACCAGAAATTTGATTTGGTTATTAAGGCTGAACAAGACAATGGTCATC CTCTTCCTGCCTTTGCCGGTCTACACATTGAAATACTGGATGAAAACAATCAAAGTCCATATTTTACAAT GCCCAGTTATCAAGGCTATATCCTGGAATCTGCCCCAGTGGGAGCAACCATTTCGGACAGTCTCAATTTG ACTTCACCTTTAAGAATAGTAGCTCTGGACAAGGACATAGAAGATACAAAAGACCCAGAGCTTCACCTTT TTCTGAATGACTACACCTCAGTCTTCACCGTCACACAGACTGGTATTACTCGCTACCTCACCTTACTTCA ACCAGTGGACAGGGAAGAACAGCAAACTTACACCTTTTCGATAACAGCATTTGATGGTGTACAAGAAAGT GAGCCAGTCATCGTCAATATTCAAGTGATGGATGCAAATGATAACACGCCAACCTTCCCTGAAATATCCT ATGATGTGTATGTTTATACAGACATGAGACCTGGGGACAGTGTCATACAGCTCACTGCAGTCGACGCAGA CGAAGGGTCAAATGGGGAGATCACATATGAAATCCTTGTTGGGGCTCAGGGAGACTTCATCATCAATAAA ACAACAGGGCTTATCACCATCGCTCCAGGGGTGGAAATGATAGTCGGGCGGACTTACGCACTCACGGTCC AAGCAGCGGATAATGCTCCTCCTGCAGAGCGAAGCACGGGGATTCTAACCTTAGGGAAAGCACTGGACAG GGAAAGCACTGATCGCTACATTCTGATCATCACAGCTTCAGATGGCAGGCCAGATGGGACCTCAACTGCC ACAGTAAACATAGTGGTGACAGATGTCAATGACAATGCTCCAGTGTTTGATCCTTATCTGCCAAGAAATT TATCTGTGGTGGAAGAAGAAGCCAATGCCTTTGTGGGTCAAGTAAAAGCAACAGACCCTGATGCTGGAAT AAATGGTCAAGTGCACTACAGTTTGGGTAACTTTAATAATCTTTTTCGTATCACATCCAATGGGAGCATT TACACAGCAGTGAAGCTTAACAGAGAAGTCAGGGACTACTATGAACTTGTTGTTGTGGCAACAGATGGAG CAGTACACCCTCGTCATTCAACTCTAACCTTGGCCATCAAGGTTTTGGACATTGATGATAACAGTCCTGT GTTCACCAATTCAACATACACTGTCCTTGTTGAAGAGAATTTGCCAGCTGGGACTACCATCCTTCAAATA GAGGCCAAAGATGTCGACCTTGGAGCAAATGTGTCTTACCGGATAAGAAGCCCAGAAGTGAAGCACTTTT TTGCACTACATCCATTTACAGGAGAACTATCGCTTTTAAGGAGTTTAGATTATGAGGCATTTCCAGACCA AGAAGCAAGTATCACTTTTCTGGTAGAGGCCTTTGATATTTATGGAACAATGCCACCTGGTATTGCTACT GTCACAGTGATTGTAAAGGATATGAATGATTATCCTCCTGTCTTTAGTAAACGAATATACAAAGGGATGG TGGCTCCGGATGCAGTCAAGGGTACACCTATCACAACAGTTTATGCTGAAGATGCAGACCCTCCTGGATT ACCTGCAAGTCGTGTGAGGTATAGAGTAGATGATGTACAGTTTCCTTACCCTGCCAGTATTTTTGAAGTG GAAGAAGATTCTGGAAGAGTAATAACACGAGTCAATCTTAATGAAGAACCTACAACAATTTTTAAGTTGG TGGTGGTTGCTTTTGATGATGGGGAGCCTGTGATGTCCAGCAGTGCCACAGTGAAGATTCTTGTCTTACA TCCTGGTGAGATCCCACGCTTCACACAGGAGGAATATAGACCTCCTCCAGTAAGTGAACTTGCCACCAAA GGGACCATGGTTGGTGTAATTTCTGCTGCTGCCATTAATCAAAGTATTGTGTACTCCATTGTTTCAGGAA ATGAAGAAGATACATTTGGAATTAATAACATCACAGGTGTTATCTATGTGAATGGACCTCTGGATTATGA GACCAGGACAAGCTATGTACTTCGAGTCCAAGCTGATTCCCTGGAAGTGGTCCTTGCCAATCTCCGAGTT CCTTCAAAAAGCAATACAGCTAAAGTATACATTGAGATTCAGGATGAAAATAATCATCCCCCAGTGTTTC AGAAAAAATTCTACATCGGAGGTGTATCTGAAGATGCAAGAATGTTTACTTCTGTACTCAGAGTGAAGGC TACTGATAAAGATACTGGCAATTATAGTGTCATGGCCTACAGACTCATAATACCACCAATTAAAGAGGGA AAAGAAGGATTTGTAGTGGAAACATATACAGGGCTTATCAAAACTGCTATGCTCTTCCATAATATGAGGA GATCCTACTTCAAGTTTCAAGTTATTGCAACTGACGACTATGGGAAGGGACTGAGCGGCAAAGCCGATGT ACTCGTCTCCGTGGTCAATCAGCTGGATATGCAAGTCATTGTTTCCAATGTGCCTCCTACTCTAGTGGAA AAAAAGATAGAAGATCTTACAGAGATCTTGGATCGCTATGTTCAGGAACAAATTCCTGGTGCCAAGGTCG TAGTGGAGTCCATTGGAGCTCGCCGGCATGGAGATGCCTTTTCCCTAGAAGATTACACCAAATGTGACTT GACTGTCTATGCAATTGACCCCCAAACCAACAGAGCCATCGATAGAAATGAGCTTTTTAAATTTTTGGAT GGCAAACTACTTGATATCAATAAAGACTTTCAGCCGTATTATGGGGAAGGAGGACGCATTCTGGAGATCC GGACTCCAGAGGCAGTGACCAGCATTAAAAAGAGAGGAGAAAGTCTAGGATACACAGAAGGGGCCTTGTT GGCTCTGGCCTTCATCATCATCCTCTGCTGCATTCCTGCCATCTTGGTGGTTTTGGTCAGCTACAGACAG TTTAAAGTACGTCAAGCTGAGTGTACAAAGACTGCACGAATTCAGGCCGCATTACCCGCGGCTAAACCAG CAGTGCCGGCTCCTGCACCAGTGGCAGCGCCCCCGCCGCCGCCGCCGCCTCCGCCAGGTGCGCATCTCTA TGAAGAACTTGGAGACAGCTCAATGCATAATCTTTTCCTTCTCTACCATTTTCAACAAAGCAGGGGAAAT AACTCAGTCTCAGAAGACAGGAAACATCAACAAGTTGTGATGCCCTTTTCTTCCAATACTATTGAGGCTC ACAAGTCAGCTCATGTAGACGGATCACTTAAGAGCAACAAACTGAAGTCTGCAAGAAAATTCACATTTCT ATCTGATGAGGATGACTTAAGTGCCCATAATCCCCTTTATAAGGAAAACATAAGTCAAGTATCAACAAAT TCAGACATTTCACAGAGAACAGATTTTGTAGACCCATTTTCACCCAAAATACAAGCCAAGAGTAAGTCTC TGAGGGGCCCAAGAGAAAAGATTCAGAGGCTGTGGAGTCAGTCAGTCAGCTTACCCAGGAGGCTGATGAG GAAAGTTCCAAATAGACCAGAGATCATAGATCTGCAGCAGTGGCAAGGCACCAGGCAGAAAGCTGAAAAT GAAAACACTGGAATCTGTACAAACAAAAGAGGTAGCAGCAATCCATTGCTTACAACTGAAGAGGCAAATT TGACAGAGAAAGAGGAAATAAGGCAAGGTGAAACACTGATGATAGAAGGAACAGAACAGTTGAAATCTCT CTCTTCAGACTCTTCATTTTGCTTTCCCAGGCCTCACTTCTCATTCTCCACTTTGCCAACTGTTTCAAGA ACTGTGGAACTCAAATCAGAACCTAATGTCATCAGTTCTCCTGCTGAGTGTTCCTTGGAACTTTCTCCTT CAAGGCCTTGTGTTTTACATTCTTCACTCTCTAGGAGAGAGACACCTATTTGTATGTTACCTATTGAAAC CGAAAGAAATATTTTTGAAAATTTTGCCCATCCACCAAACATCTCTCCTTCTGCCTGTCCCCTTCCCCCT CCTCCTCCTATTTCTCCTCCTTCTCCTCCTCCTGCTCCTGCTCCTCTTGCTCCTCCTCCTGACATTTCTC CTTTTTCTCTTTTTTGTCCTCCTCCCTCTCCTCCTTCTATCCCTCTTCCTCTTCCTCCTCCTACATTTTT TCCACTTTCCGTTTCAACGTCTGGTCCCCCAACACCACCTCTTCTACCTCCATTTCCAACTCCTCTTCCT CCACCACCTCCTTCTATTCCTTGCCCTCCACCTCCTTCAGCTTCATTTCTGTCCACAGAGTGTGTCTGTA TAACAGGTGTTAAATGCACGACCAACTTGATGCCTGCCGAGAAAATTAAGTCCTCTATGACACAGCTATC AACAACGACAGTGTGTAAAACAGACCCTCAGAGAGAACCAAAAGGCATCCTCAGACACGTTAAAAACTTA GCAGAACTTGAAAAATCAGTAGCTAACATGTACAGTCAAATAGAAAAAAACTATCTACGCACAAATGTTT CAGAACTTCAAACTATGTGCCCTTCAGAAGTAACAAATATGGAAATCACATCTGAACAAAACAAGGGGAG TTTGAACAATATTGTCGAGGGAACTGAAAAACAATCTCACAGTCAATCTACTTCACTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC226598 representing NM_001142765
Red=Cloning site Green=Tags(s) MFRQFYLWTCLASGIILGSLFEICLGQYDDDCKLARGGPPATIVAIDEESRNGTILVDNMLIKGTAGGPD PTIELSLKDNVDYWVLMDPVKQMLFLNSTGRVLDRDPPMNIHSIVVQVQCINKKVGTIIYHEVRIVVRDR NDNSPTFKHESYYATVNELTPVGTTIFTGFSGDNGATDIDDGPNGQIEYVIQYNPDDPTSNDTFEIPLML TGNIVLRKRLNYEDKTRYFVIIQANDRAQNLNERRTTTTTLTVDVLDGDDLGPMFLPCVLVPNTRDCRPL TYQAAIPELRTPEELNPIIVTPPIQAIDQDRNIQPPSDRPGILYSILVGTPEDYPRFFHMHPRTAELSLL EPVNRDFHQKFDLVIKAEQDNGHPLPAFAGLHIEILDENNQSPYFTMPSYQGYILESAPVGATISDSLNL TSPLRIVALDKDIEDTKDPELHLFLNDYTSVFTVTQTGITRYLTLLQPVDREEQQTYTFSITAFDGVQES EPVIVNIQVMDANDNTPTFPEISYDVYVYTDMRPGDSVIQLTAVDADEGSNGEITYEILVGAQGDFIINK TTGLITIAPGVEMIVGRTYALTVQAADNAPPAERSTGILTLGKALDRESTDRYILIITASDGRPDGTSTA TVNIVVTDVNDNAPVFDPYLPRNLSVVEEEANAFVGQVKATDPDAGINGQVHYSLGNFNNLFRITSNGSI YTAVKLNREVRDYYELVVVATDGAVHPRHSTLTLAIKVLDIDDNSPVFTNSTYTVLVEENLPAGTTILQI EAKDVDLGANVSYRIRSPEVKHFFALHPFTGELSLLRSLDYEAFPDQEASITFLVEAFDIYGTMPPGIAT VTVIVKDMNDYPPVFSKRIYKGMVAPDAVKGTPITTVYAEDADPPGLPASRVRYRVDDVQFPYPASIFEV EEDSGRVITRVNLNEEPTTIFKLVVVAFDDGEPVMSSSATVKILVLHPGEIPRFTQEEYRPPPVSELATK GTMVGVISAAAINQSIVYSIVSGNEEDTFGINNITGVIYVNGPLDYETRTSYVLRVQADSLEVVLANLRV PSKSNTAKVYIEIQDENNHPPVFQKKFYIGGVSEDARMFTSVLRVKATDKDTGNYSVMAYRLIIPPIKEG KEGFVVETYTGLIKTAMLFHNMRRSYFKFQVIATDDYGKGLSGKADVLVSVVNQLDMQVIVSNVPPTLVE KKIEDLTEILDRYVQEQIPGAKVVVESIGARRHGDAFSLEDYTKCDLTVYAIDPQTNRAIDRNELFKFLD GKLLDINKDFQPYYGEGGRILEIRTPEAVTSIKKRGESLGYTEGALLALAFIIILCCIPAILVVLVSYRQ FKVRQAECTKTARIQAALPAAKPAVPAPAPVAAPPPPPPPPPGAHLYEELGDSSMHNLFLLYHFQQSRGN NSVSEDRKHQQVVMPFSSNTIEAHKSAHVDGSLKSNKLKSARKFTFLSDEDDLSAHNPLYKENISQVSTN SDISQRTDFVDPFSPKIQAKSKSLRGPREKIQRLWSQSVSLPRRLMRKVPNRPEIIDLQQWQGTRQKAEN ENTGICTNKRGSSNPLLTTEEANLTEKEEIRQGETLMIEGTEQLKSLSSDSSFCFPRPHFSFSTLPTVSR TVELKSEPNVISSPAECSLELSPSRPCVLHSSLSRRETPICMLPIETERNIFENFAHPPNISPSACPLPP PPPISPPSPPPAPAPLAPPPDISPFSLFCPPPSPPSIPLPLPPPTFFPLSVSTSGPPTPPLLPPFPTPLP PPPPSIPCPPPPSASFLSTECVCITGVKCTTNLMPAEKIKSSMTQLSTTTVCKTDPQREPKGILRHVKNL AELEKSVANMYSQIEKNYLRTNVSELQTMCPSEVTNMEITSEQNKGSLNNIVEGTEKQSHSQSTSL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001142765 |
ORF Size | 5658 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001142765.2 |
RefSeq ORF | 5661 bp |
Locus ID | 65217 |
Protein Families | Druggable Genome, Transmembrane |
MW | 208.36 kDa |
Gene Summary | This gene is a member of the cadherin superfamily. Family members encode integral membrane proteins that mediate calcium-dependent cell-cell adhesion. It plays an essential role in maintenance of normal retinal and cochlear function. Mutations in this gene result in hearing loss and Usher Syndrome Type IF (USH1F). Extensive alternative splicing resulting in multiple isoforms has been observed in the mouse ortholog. Similar alternatively spliced transcripts are inferred to occur in human, and additional variants are likely to occur. [provided by RefSeq, Dec 2008] |
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