LSS (NM_001145436) Human Tagged ORF Clone
CAT#: RC226713
- TrueORF®
LSS (Myc-DDK-tagged)-Human lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase) (LSS), transcript variant 3
ORF Plasmid: tGFP
"NM_001145436" in other vectors (4)
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CNY 7,600.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | APMR4; CTRCT44; HYPT14; OSC |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC226713 representing NM_001145436
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGGAGGGCACGTGTCTGCGGCGCCGAGGGGGCCCCTACAAGACCGAGCCCGCCACCGACCTCGGCC GCTGGCGACTCAACTGCGAGAGGGGCCGGCAGACGTGGACCTACCTGCAGGACGAGCGCGCCGGCCGCGA GCAGACCGGCCTGGAAGCCTACGCCCTGGGGCTGGACACCAAGAATTACTTTAAGGACTTGCCCAAAGCC CACACCGCCTTTGAGGGGGCTCTGAACGGGATGACATTTTACGTGGGGCTGCAGGCTGAGGATGGGCACT GGACGGGTGATTATGGTGGCCCACTTTTCCTCCTGCCAGGCCTCCTGATCACTTGCCACGTGGCACGCAT CCCTCTGCCAGCCGGATACAGAGAAGAGATTGTGCGGTACCTGCGGCACATTGAGGATAAGTCCACCGTG TTTGGGACTGCGCTCAACTATGTGTCTCTCAGAATTCTGGGTGTTGGGCCTGACGATCCTGACCTGGTAC GAGCCCGGAACATTCTTCACAAGAAAGGTGGTGCTGTGGCCATCCCCTCCTGGGGGAAGTTCTGGCTGGC TGTCCTGAATGTTTACAGCTGGGAAGGCCTCAATACCCTGTTCCCAGAGATGTGGCTGTTTCCTGACTGG GCACCGGCACACCCCTCCACACTCTGGTGCCACTGCCGGCAGGTGTACCTGCCCATGAGCTACTGCTACG CCGTTCGGCTGAGTGCCGCGGAAGACCCGCTGGTCCAGAGCCTCCGCCAGGAGCTCTATGTGGAGGACTT CGCCAGCATTGACTGGCTGGCGCAGAGGAACAACGTGGCCCCCGACGAGCTGTACACGCCGCACAGCTGG CTGCTCCGCGTGGTATATGCGCTCCTCAACCTGTATGAGCACCACCACAGTGCCCACCTGCGGCAGCGGG CCGTGCAGAAGCTGTATGAACACATTGTGGCCGACGACCGATTCACCAAGAGCATCAGCATCGGCCCGAT CTCGAAAACCATCAACATGCTTGTGCGCTGGTATGTGGACGGGCCCGCCTCCACTGCCTTCCAGGAGCAT GTCTCCAGAATCCCGGACTATCTCTGGATGGGCCTTGACGGCATGAAAATGCAGGGCACCAACGGCTCAC AGATCTGGGACACCGCATTCGCCATCCAGGCTCTGCTTGAGGCGGGCGGGCACCACAGGCCCGAGTTTTC GTCCTGCCTGCAGAAGGCTCATGAGTTCCTGAGGCTCTCACAGGTCCCAGATAACCCTCCCGACTACCAG AAGTACTACCGCCAGATGCGCAAGGGTGGCTTCTCCTTCAGTACGCTGGACTGCGGCTGGATCGTTTCTG ACTGCACGGCTGAGGCCTTGAAGGCTGTGCTGCTCCTGCAGGAGAAGTGTCCCCATGTCACCGAGCACAT CCCCAGAGAACGGCTCTGCGATGCTGTGGCTGTGCTGCTGAACATGAGAAATCCAGATGGAGGGTTCGCC ACCTATGAGACCAAGCGTGGGGGGCACTTGCTGGAGCTGCTGAACCCCTCGGAGGTCTTCGGGGACATCA TGATTGACTACACCTATGTGGAGTGCACCTCAGCCGTGATGCAGGCGCTTAAGTATTTCCACAAGCGTTT CCCGGAGCACAGGGCAGCGGAGATCCGGGAGACCCTCACGCAGGGCTTAGAGTTCTGTCGGCGGCAGCAG AGGGCCGATGGCTCCTGGGAAGGCTCCTGGGGAGTTTGCTTCACCTACGGCACCTGGTTTGGCCTGGAGG CCTTCGCCTGTATGGGGCAGACCTACCGAGATGGGACTGCCTGTGCAGAGGTCTCCCGGGCCTGTGACTT CCTGCTGTCCCGGCAGATGGCAGACGGAGGCTGGGGGGAGGACTTTGAGTCCTGCGAGGAGCGGCGTTAT TTGCAGAGTGCCCAGTCCCAGATCCATAACACATGCTGGGCCATGATGGGGCTGATGGCCGTTCGGCATC CTGACATCGAGGCCCAGGAGAGAGGAGTCCGGTGTCTACTTGAGAAACAGCTCCCCAATGGCGACTGGCC GCAGGAAAACATTGCTGGGGTCTTCAACAAGTCCTGTGCCATCTCCTACACGAGCTACAGGAACATCTTC CCCATCTGGGCCCTCGGCCGCTTCTCCCAGCTGTACCCTGAGAGAGCCCTTGCTGGCCACCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC226713 representing NM_001145436
Red=Cloning site Green=Tags(s) MTEGTCLRRRGGPYKTEPATDLGRWRLNCERGRQTWTYLQDERAGREQTGLEAYALGLDTKNYFKDLPKA HTAFEGALNGMTFYVGLQAEDGHWTGDYGGPLFLLPGLLITCHVARIPLPAGYREEIVRYLRHIEDKSTV FGTALNYVSLRILGVGPDDPDLVRARNILHKKGGAVAIPSWGKFWLAVLNVYSWEGLNTLFPEMWLFPDW APAHPSTLWCHCRQVYLPMSYCYAVRLSAAEDPLVQSLRQELYVEDFASIDWLAQRNNVAPDELYTPHSW LLRVVYALLNLYEHHHSAHLRQRAVQKLYEHIVADDRFTKSISIGPISKTINMLVRWYVDGPASTAFQEH VSRIPDYLWMGLDGMKMQGTNGSQIWDTAFAIQALLEAGGHHRPEFSSCLQKAHEFLRLSQVPDNPPDYQ KYYRQMRKGGFSFSTLDCGWIVSDCTAEALKAVLLLQEKCPHVTEHIPRERLCDAVAVLLNMRNPDGGFA TYETKRGGHLLELLNPSEVFGDIMIDYTYVECTSAVMQALKYFHKRFPEHRAAEIRETLTQGLEFCRRQQ RADGSWEGSWGVCFTYGTWFGLEAFACMGQTYRDGTACAEVSRACDFLLSRQMADGGWGEDFESCEERRY LQSAQSQIHNTCWAMMGLMAVRHPDIEAQERGVRCLLEKQLPNGDWPQENIAGVFNKSCAISYTSYRNIF PIWALGRFSQLYPERALAGHP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001145436 |
ORF Size | 2163 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001145436.2 |
RefSeq ORF | 2166 bp |
Locus ID | 4047 |
UniProt ID | P48449 |
Protein Families | Druggable Genome |
Protein Pathways | Metabolic pathways, Steroid biosynthesis |
MW | 82 kDa |
Gene Summary | The protein encoded by this gene catalyzes the conversion of (S)-2,3 oxidosqualene to lanosterol. The encoded protein is a member of the terpene cyclase/mutase family and catalyzes the first step in the biosynthesis of cholesterol, steroid hormones, and vitamin D. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Feb 2009] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC226713L3 | Lenti-ORF clone of LSS (Myc-DDK-tagged)-Human lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase) (LSS), transcript variant 3 |
CNY 9,500.00 |
|
RC226713L4 | Lenti-ORF clone of LSS (mGFP-tagged)-Human lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase) (LSS), transcript variant 3 |
CNY 9,500.00 |
|
RG226713 | LSS (tGFP-tagged) - Human lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase) (LSS), transcript variant 3 |
CNY 8,360.00 |
|
SC326617 | LSS (untagged)-Human lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase) (LSS), transcript variant 3, mRNA |
CNY 13,870.00 |