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PRDM6 (NM_001136239) Human Tagged ORF Clone
CAT＃: RC227008
- TrueORF®
PRDM6 (Myc-DDK-tagged)-Human PR domain containing 6 (PRDM6)
ORF Plasmid: tGFP
"NM_001136239" in other vectors (4)
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CNY 6,648.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | KMT8C; PDA3; PRISM |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC227008 representing NM_001136239
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGAAGCCCGGAGACCCCGGCGGTTCGGCCTTCCTCAAAGTGGACCCAGCCTACCTGCAGCACTGGC AGCAACTCTTCCCTCACGGAGGCGCAGGCCCGCTCAAGGGCAGCGGCGCCGCGGGTCTCCTGAGCGCGCC GCAGCCTCTTCAGCCGCCGCCGCCGCCCCCGCCCCCGGAGCGCGCTGAGCCTCCGCCGGACAGCCTGCGC CCGCGGCCCGCCTCTCTCTCCTCCGCCTCGTCCACGCCGGCTTCCTCTTCCACCTCCGCCTCCTCCGCCT CCTCCTGCGCTGCTGCGGCCGCTGCCGCCGCGCTGGCTGGTCTCTCGGCCCTGCCGGTGTCGCAGCTGCC GGTGTTCGCGCCTCTAGCCGCCGCTGCCGTCGCCGCCGAGCCGCTGCCCCCCAAGGAACTGTGCCTCGGC GCCACCTCCGGCCCCGGGCCCGTCAAGTGCGGTGGTGGTGGCGGCGGCGGCGGGGAGGGTCGCGGCGCCC CGCGCTTCCGCTGCAGCGCAGAGGAGCTGGACTATTACCTGTATGGCCAGCAGCGCATGGAGATCATCCC GCTCAACCAGCACACCAGCGACCCCAACAACCGTTGCGACATGTGCGCGGACAACCGCAACGGCGAGTGC CCTATGCATGGGCCACTGCACTCGCTGCGCCGGCTTGTGGGCACCAGCAGCGCTGCGGCCGCCGCGCCCC CGCCGGAGCTGCCGGAGTGGCTGCGGGACCTGCCTCGCGAGGTGTGCCTCTGCACCAGTACTGTGCCCGG CCTGGCCTACGGCATCTGCGCGGCGCAGAGGATCCAGCAAGGCACCTGGATTGGACCTTTCCAAGGCGTG CTTCTGCCCCCAGAGAAGGTGCAGGCAGGCGCCGTGAGGAACACGCAGCATCTCTGGGAGATATATGACC AGGATGGGACACTACAGCACTTTATTGATGGTGGGGAACCTAGTAAGTCGAGCTGGATGAGGTATATCCG ATGTGCAAGGCACTGCGGAGAACAGAATCTAACAGTAGTTCAGTACAGGTCGAATATATTCTACCGAGCC TGTATAGATATCCCTAGGGGCACCGAGCTTCTGGTGTGGTACAATGACAGCTATACGTCTTTCTTTGGGA TCCCCTTACAATGCATTGCCCAGGATGAAAACTTAAATGTCCCTTCAACGGTAATGGAAGCCATGTGCAG ACAAGACGCCCTGCAGCCCTTCAACAAAAGCAGCAAACTCGCCCCTACCACCCAGCAGCGCTCCGTTGTT TTCCCCCAGACTCCGTGCAGCAGGAACTTCTCTCTTCTGGATAAGTCTGGGCCCATTGAATCAGGATTTA ATCAAATCAACGTGAAAAACCAGCGAGTCCTGGCAAGCCCAACTTCCACAAGCCAGCTCCACTCGGAGTT CAGTGACTGGCATCTTTGGAAATGTGGGCAGTGCTTTAAGACTTTCACCCAGCGGATCCTCTTACAGATG CACGTGTGCACGCAGAACCCCGACAGACCCTACCAATGCGGCCACTGCTCCCAGTCCTTTTCCCAGCCTT CAGAACTGAGGAACCACGTGGTCACTCACTCTAGTGACCGGCCTTTCAAGTGCGGCTACTGTGGTCGTGC CTTTGCCGGGGCCACCACCCTCAACAACCACATCCGAACCCACACTGGAGAAAAGCCCTTCAAGTGCGAG AGGTGTGAGAGGAGCTTCACGCAGGCCACCCAGCTGAGCCGACACCAGCGGATGCCCAATGAGTGCAAGC CAATAACTGAGAGCCCAGAATCAATCGAAGTGGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC227008 representing NM_001136239
Red=Cloning site Green=Tags(s) MLKPGDPGGSAFLKVDPAYLQHWQQLFPHGGAGPLKGSGAAGLLSAPQPLQPPPPPPPPERAEPPPDSLR PRPASLSSASSTPASSSTSASSASSCAAAAAAAALAGLSALPVSQLPVFAPLAAAAVAAEPLPPKELCLG ATSGPGPVKCGGGGGGGGEGRGAPRFRCSAEELDYYLYGQQRMEIIPLNQHTSDPNNRCDMCADNRNGEC PMHGPLHSLRRLVGTSSAAAAAPPPELPEWLRDLPREVCLCTSTVPGLAYGICAAQRIQQGTWIGPFQGV LLPPEKVQAGAVRNTQHLWEIYDQDGTLQHFIDGGEPSKSSWMRYIRCARHCGEQNLTVVQYRSNIFYRA CIDIPRGTELLVWYNDSYTSFFGIPLQCIAQDENLNVPSTVMEAMCRQDALQPFNKSSKLAPTTQQRSVV FPQTPCSRNFSLLDKSGPIESGFNQINVKNQRVLASPTSTSQLHSEFSDWHLWKCGQCFKTFTQRILLQM HVCTQNPDRPYQCGHCSQSFSQPSELRNHVVTHSSDRPFKCGYCGRAFAGATTLNNHIRTHTGEKPFKCE RCERSFTQATQLSRHQRMPNECKPITESPESIEVD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001136239 |
| ORF Size | 1785 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001136239.4 |
| RefSeq ORF | 1788 bp |
| Locus ID | 93166 |
| UniProt ID | Q9NQX0 |
| MW | 64.3 kDa |
| Gene Summary | The protein encoded by this gene is a transcriptional repressor and a member of the PRDM family. Family members contain a PR domain and multiple zinc-finger domains. The encoded protein is involved in regulation of vascular smooth muscle cells (VSMC) contractile proteins. Mutations in this gene result in patent ductus arteriosus 3 (PDA3). [provided by RefSeq, Apr 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC227008L3 | Lenti-ORF clone of PRDM6 (Myc-DDK-tagged)-Human PR domain containing 6 (PRDM6) |
CNY 6,460.00 |
|
| RC227008L4 | Lenti-ORF clone of PRDM6 (mGFP-tagged)-Human PR domain containing 6 (PRDM6) |
CNY 6,460.00 |
|
| RG227008 | PRDM6 (tGFP-tagged) - Human PR domain containing 6 (PRDM6) |
CNY 5,040.00 |
|
| SC326096 | PRDM6 (untagged)-Human PR domain containing 6 (PRDM6) |
CNY 6,656.00 |
