EXOC7 (NM_001145298) Human Tagged ORF Clone
CAT#: RC227511
EXOC7 (Myc-DDK-tagged)-Human exocyst complex component 7 (EXOC7), transcript variant 5
ORF Plasmid: tGFP
"NM_001145298" in other vectors (4)
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CNY 4,952.00
CNY 7,220.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 2-5-3p; BLOM4; EX070; EXO70; Exo70p; EXOC1; NEDSEBA; YJL085W |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC227511 representing NM_001145298
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTCCCCCACAGGAGGCATCCGCTCGACGGCGGGAGATTGAGGACAAGCTGAAGCAGGAGGAGGAGA CTCTGTCCTTCATCCGAGACAGCCTGGAGAAGAGCGACCAGCTCACTAAGAACATGGTGTCTATCTTATC ATCCTTTGAGAGCCGCCTTATGAAGCTGGAGAACTCCATCATCCCTGTGCACAAGCAGACGGAGAATCTG CAGCGGCTGCAGGAGAATGTTGAGAAGACGCTGTCCTGCCTGGACCATGTCATCAGCTACTACCATGTGG CCAGTGACACTGAGAAGATCATCAGAGAGGGCCCCACAGGTAGGCTGGAAGAGTACCTGGGAAGCATGGC CAAGATTCAGAAGGCAGTGGAGTATTTCCAGGACAACAGCCCAGACAGCCCGGAACTCAACAAAGTGAAA CTGCTCTTTGAGCGCGGGAAGGAGGCCCTGGAGTCCGAATTTCGCAGCCTGATGACGCGGCACAGTAAGG TCGTCTCGCCCGTGCTCATCTTGGATCTGATCAGTGGTGACGATGATCTGGAGGCCCAGGAGGACGTGAC CCTGGAGCACCTGCCCGAGAGCGTGCTCCAGGATGTCATTCGCATCTCCCGCTGGCTGGTGGAATATGGC CGCAACCAAGATTTCATGAACGTCTACTACCAGATACGCTCCAGCCAGCTGGACCGCTCCATCAAAGGAC TGAAGGAGCATTTCCATAAGAGCAGTTCTTCCTCTGGGGTTCCCTACTCCCCTGCTATCCCCAACAAGAG GAAAGACACACCTACCAAGAAGCCAGTCAAGCGGCCAGGTCACGAGCATGATTTCCGAGTTAAGCACCTG TCCGAGGCCTTGAACGACAAGCACGGGCCGCTGGCCGGGAGAGATGACATGCTGGACGTGGAGACCGATG CCTACATCCACTGCGTCAGTGCCTTCGTCAAGCTGGCGCAGAGCGAGTACCAGCTGCTGGCCGACATCAT CCCCGAGCACCACCAGAAGAAGACCTTCGACTCCCTGATACAGGATGCCCTGGATGGGCTGATGCTTGAA GGGGAGAACATCGTGTCTGCTGCCCGGAAGGCCATTGTGCGACACGACTTCTCCACGGTGCTCACCGTCT TCCCCATCCTGCGACACCTCAAGCAGACCAAGCCTGAGTTTGACCAGGTGCTCCAGGGCACGGCTGCCAG CACAAAGAACAAGCTGCCTGGCCTCATCACATCCATGGAGACCATCGGTGCCAAAGCGCTGGAGGACTTC GCAGACAACATCAAGAATGACCCGGACAAGGAGTACAACATGCCGAAGGACGGCACCGTACACGAGCTCA CCAGCAATGCCATCCTCTTCCTGCAGCAGCTTTTGGACTTCCAGGAGACGGCAGGCGCCATGCTGGCCTC CCAAGAGACCAGCTCTTCGGCCACCAGCTACAGCTCTGAGTTCAGCAAGCGGCTGCTAAGCACCTATATC TGTAAAGTGCTGGGCAACCTGCAGTTGAACTTGCTGAGCAAGTCCAAGGTGTACGAGGACCCAGCTCTGA GCGCCATCTTCCTGCACAACAACTACAATTACATCCTCAAGTCCCTGGAGAAGTCTGAACTGATCCAGCT GGTGGCAGTGACACAGAAGACTGCTGAGCGCTCCTACCGGGAGCACATTGAGCAGCAGATCCAGACCTAC CAGCGCAGCTGGTTAAAGGTGACTGATTACATCGCAGAGAAGAATCTACCTGTGTTCCAGCCGGGAGTCA AGCTCCGGGACAAGGAGCGGCAGATTATCAAGGAGCGTTTTAAGGGCTTCAATGATGGCCTCGAAGAACT GTGCAAAATCCAGAAGGCCTGGGCTATTCCAGACACAGAGCAGAGGGACAGGATTCGCCAGGCCCAGAAG ACCATTGTCAAGGAGACCTACGGGGCCTTTCTACAGAAGTTTGGCAGCGTGCCCTTCACCAAGAACCCGG AGAAGTACATCAAGTACGGGGTGGAGCAGGTGGGCGACATGATCGATCGCCTTTTCGACACCTCTGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC227511 representing NM_001145298
Red=Cloning site Green=Tags(s) MIPPQEASARRREIEDKLKQEEETLSFIRDSLEKSDQLTKNMVSILSSFESRLMKLENSIIPVHKQTENL QRLQENVEKTLSCLDHVISYYHVASDTEKIIREGPTGRLEEYLGSMAKIQKAVEYFQDNSPDSPELNKVK LLFERGKEALESEFRSLMTRHSKVVSPVLILDLISGDDDLEAQEDVTLEHLPESVLQDVIRISRWLVEYG RNQDFMNVYYQIRSSQLDRSIKGLKEHFHKSSSSSGVPYSPAIPNKRKDTPTKKPVKRPGHEHDFRVKHL SEALNDKHGPLAGRDDMLDVETDAYIHCVSAFVKLAQSEYQLLADIIPEHHQKKTFDSLIQDALDGLMLE GENIVSAARKAIVRHDFSTVLTVFPILRHLKQTKPEFDQVLQGTAASTKNKLPGLITSMETIGAKALEDF ADNIKNDPDKEYNMPKDGTVHELTSNAILFLQQLLDFQETAGAMLASQETSSSATSYSSEFSKRLLSTYI CKVLGNLQLNLLSKSKVYEDPALSAIFLHNNYNYILKSLEKSELIQLVAVTQKTAERSYREHIEQQIQTY QRSWLKVTDYIAEKNLPVFQPGVKLRDKERQIIKERFKGFNDGLEELCKIQKAWAIPDTEQRDRIRQAQK TIVKETYGAFLQKFGSVPFTKNPEKYIKYGVEQVGDMIDRLFDTSA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001145298 |
ORF Size | 2028 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001145298.4 |
RefSeq ORF | 2031 bp |
Locus ID | 23265 |
UniProt ID | Q9UPT5 |
Protein Families | Druggable Genome |
Protein Pathways | Insulin signaling pathway |
MW | 77.1 kDa |
Gene Summary | The protein encoded by this gene is a component of the exocyst complex. The exocyst complex plays a critical role in vesicular trafficking and the secretory pathway by targeting post-Golgi vesicles to the plasma membrane. The encoded protein is required for assembly of the exocyst complex and docking of the complex to the plasma membrane. The encoded protein may also play a role in pre-mRNA splicing through interactions with pre-mRNA-processing factor 19. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 4. [provided by RefSeq, Nov 2011] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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SmartBac, a new baculovirus system for large protein complex production
,Zhai, Y;Zhang, D;Yu, L;Sun, F;Sun, F;,
Journal of Structural Biology: X
[EXOC7]
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Documents
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC227511L3 | Lenti-ORF clone of EXOC7 (Myc-DDK-tagged)-Human exocyst complex component 7 (EXOC7), transcript variant 5 |
CNY 9,120.00 |
|
RC227511L4 | Lenti-ORF clone of EXOC7 (mGFP-tagged)-Human exocyst complex component 7 (EXOC7), transcript variant 5 |
CNY 9,120.00 |
|
RG227511 | EXOC7 (tGFP-tagged) - Human exocyst complex component 7 (EXOC7), transcript variant 5 |
CNY 7,980.00 |
|
SC326166 | EXOC7 (untagged)-Human exocyst complex component 7 (EXOC7), transcript variant 5 |
CNY 13,110.00 |