ZNF534 (NM_001143938) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	KRBO3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >NM_001143938 ORF sequence, RC227935 may differ due to SNPs. Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCCCTTACTCAGGGGCAATTGTCATTCAGCGATGTGGCCATAGAATTCTCTCAGGAGGAGTGGAAA TGCCTGGACCCTGGGCAGAAAGCTTTATACAGGGACGTGATGTTAGAGAACTACAGGAACCTGGTCTCC CTAGGAATCTGTCTTCCTGACCTGAGTGTTACCTCCATGTTGGAGCAAAAGAGAGATCCCTGGACTCTG CAGAGTGAAGTGAAAATAATAAACAATCCAGATGGCAGGGAGTGCATCAAAGGTGTGAACACAGAGAAG AGTTCTAAATTGGGAAGCAGTGCAGGAAACAAGTCACTTAAAAATCAACATGGATTAACTCTTCAGTTA CATCTGACTGAATGGCAGCCATTTCAAGCTGTAAGGAATATTTATGGATGTAAGCATGTTGAGAAATCT ATCAGTGACAATTCTTCAGTTTCACCAGTTCAAATAAGTTTTTTCAGTGTCAAAACCCATATTTTTAAT AACTACAGAAATGATTTTCTTTTTTCTACATTACTCCCACAAGAACAGAAAGTACACATTAGGGAAAAG CCTTATGGATGTAATGAGCATGGGAAAGTCTTCAGAGTGTCTTCAAGCCTTACTAACCGTCAAGTAATC CACATTGCAGATAAAACTTACAAATGTAGTGACTGTGGCGAGATCTTTAGTAGCAATTCAAACTTTGCA CAACATCAACGAATCCATACTGGAGAGAAGCCTTACAAATATAATGAATGTGGCAAAGTCTTCAATCAG AATTCACACCTTGCACAACATCAGAAAATTCATACTGGACAGAAACCTTACAATAACAAAGAATGTGGG AAAGTCTTTAGTCACCATGCCTACCTTGCACAGCATAGGAAAATTCATACTGGAGAGAAGCCTTACAAA TGTAGTGAATGTGGCAAAGCATTTAGTGTGTGTTCCAGTCTTACTGCTCATCTTGTAATCCATACTGGA GAGAAACCTTATGATTGTAAGGAATGTGGCAAGGTCTTCAGGCATAAGTCTTCCCTAACCACTCATCAG ACAGTTCATACTGGAGAGAGACCATACAAATGTAATGAATGTGGCAAGGGGTTTAGTCGAATTGCATTC CTTGCAAGGCATCGGAAAGTTCATACTGGAGAGAAACCTTACAAATGTAATGAGTGTGGCAAGGTCTTT ATTGGCAATTCACGCCTTGCACGACATAGGAAAATTCATACTGGGGGGAGGCGTTACAAATGTAATGAA TGTGGCAAAGCATTTAGAACGTGTTCAGATCTCACTGCCCATCTTCTAATCCATACTGGAGAGAAACCT TACGAATGTATAGACTGTGGCAAGGTCTTCAGGCACAAGTCTTCCCTAACCTATCACTGTAGAATTCAT ACTGGAGAGAAGCCTTACAAATGTAACGAATGTGGCAAGGTCTTCAGTCAGAATTCAAACCTTCAACGA CATAGGAAAATTCATACTGGAGAGAAGCTTTACAAATGTAATGAATGTGGCAAGGTCTTCCGTCAGAAT TCACACCTTGCACAACATAGGGATATTCATACTGGAGAGAAGCCTTACAGTTGTAATGAATGTGGCAAG GTCTTCCGTCGGAATTCACACCTTGTGCGACATAGGAATGTTCATACTGGAGAAAAGCCTTACAGTTGT AATGAATGTGGCAAGGTCTTCAGTCGGAATTCACACCTTGCGCGACATAGGAATATTCATACTGGAGAG AAGCCTCACAGTTGTAATGAATGTGGCAAGGTCTTCAGTCGGAATTCACACCTTGCGCGACATAGGAAA ATTCATACTGGAGAGAAGCTTTACAAATGTAATGAATGTAGCAAGGTCTTCAGTCGGAATTCACGCCTT GCACAACATAGGAATATTCATACTGGAGTGAAGCCTTACAGTTGTAATGAATGTGGCAAGGTCTTTAGT AAAAATTCCATCCTAGTACAACATTGCAGTATTCATACCAGAGAGAAGCCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC Protein Sequence (showhide) >Peptide sequence encoded by RC227935 Blue=ORF Red=Cloning site Green=Tag(s) MALTQGQLSFSDVAIEFSQEEWKCLDPGQKALYRDVMLENYRNLVSLGICLPDLSVTSMLEQKRDPWTL QSEVKIINNPDGRECIKGVNTEKSSKLGSSAGNKSLKNQHGLTLQLHLTEWQPFQAVRNIYGCKHVEKS ISDNSSVSPVQISFFSVKTHIFNNYRNDFLFSTLLPQEQKVHIREKPYGCNEHGKVFRVSSSLTNRQVI HIADKTYKCSDCGEIFSSNSNFAQHQRIHTGEKPYKYNECGKVFNQNSHLAQHQKIHTGQKPYNNKECG KVFSHHAYLAQHRKIHTGEKPYKCSECGKAFSVCSSLTAHLVIHTGEKPYDCKECGKVFRHKSSLTTHQ TVHTGERPYKCNECGKGFSRIAFLARHRKVHTGEKPYKCNECGKVFIGNSRLARHRKIHTGGRRYKCNE CGKAFRTCSDLTAHLLIHTGEKPYECIDCGKVFRHKSSLTYHCRIHTGEKPYKCNECGKVFSQNSNLQR HRKIHTGEKLYKCNECGKVFRQNSHLAQHRDIHTGEKPYSCNECGKVFRRNSHLVRHRNVHTGEKPYSC NECGKVFSRNSHLARHRNIHTGEKPHSCNECGKVFSRNSHLARHRKIHTGEKLYKCNECSKVFSRNSRL AQHRNIHTGVKPYSCNECGKVFSKNSILVQHCSIHTREKP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001143938
ORF Size	1983 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001143938.3
RefSeq Size	5203 bp
RefSeq ORF	1986 bp
Locus ID	147658
UniProt ID	Q76KX8
MW	75.8 kDa
Gene Summary	May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function]

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