SH2D2A (NM_001161442) Human Tagged ORF Clone

CAT#: RC228298

SH2D2A (Myc-DDK-tagged)-Human SH2 domain containing 2A (SH2D2A), transcript variant 3

ORF Plasmid: DDK tGFP



  "NM_001161442" in other vectors (4)

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CNY 3,656.00

CNY 4,370.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


SH2D2A mouse monoclonal antibody, clone OTI4F3 (formerly 4F3)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms F2771; SCAP; TSAD; VRAP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC228298 representing NM_001161442
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCCCAAGCACCTTCCAGATCACAGACATGACCCGCAGGAGCTGCCAGAACCTGGGCTACACTGCGG
CATCTCCCCAGGCCCCGGAGGCTGCCTCCAACACAGGGAATGCTGAGAGGGCAGAGGAGGTGCCTGGAGA
AGGAAGCCTGTTCCTGCAGGCCGAGACCCGGGCTTGGTTCCAGAAGACCCAGGCCCACTGGCTCCTGCAG
CACGGGGCAGCCCCTGCCTGGTTCCATGGCTTCATCACCCGGAGGGAGGCAGAGAGGCTGCTGGAGCCCA
AGCCTCAGGGGTGCTACTTGGTGCGGTTCAGCGAGAGCGCGGTGACCTTCGTGCTGACTTACAGGAGCCG
GACTTGCTGCCGCCACTTCCTGCTGGCCCAGCTCAGGGACGGGCGCCACGTGGTGCTGGGCGAGGACAGC
GCCCACGCGCGGCTGCAGGACCTGCTGCTGCACTACACCGCGCACCCGCTCAGCCCCTACGGGGAGACGC
TCACCGAGCCCCTCGCCCGACAGACTCCTGAGCCTGCAGGACTTTCCCTGAGGACCGAAGAATCAAACTT
TGGAAGCAAAAGCCAGGACCCAAACCCCCAGTACAGCCCAATCATCAAACAGGGGCAAGCCCCAGTCCCG
ATGCAGAAAGAGGGGGCCGGGGAGAAGGAGCCCTCCCAGCTGCTCAGGCCCAAGCCTCCCATCCCCGCCA
AACCTCAGCTGCCCCCAGAAGTCTACACAATCCCTGTTCCACGACACCGCCCGGCCCCACGCCCCAAGCC
CTCCAATCCTATCTACAATGAGCCTGATGAACCCATAGCTTTCTATGCCATGGGCCGGGGCAGCCCTGGG
GAAGCCCCCAGCAACATCTATGTGGAAGTGGAAGATGAGGGCCTACCCGCCACCCTTGGGCACCCTGTCC
TACGGAAGAGCTGGTCCAGGCCTGTCCCAGGAGGCCAGAATACAGGTGGCTCCCAGCTGCATTCTGAGAA
CTCTGTGATTGGGCAAGGCCCTCCCCTGCCCCACCAGCCCCCACCCGCCTGGAGACACACCCTCCCCCAC
AATCTTTCTAGACAGGTGCTTCAGGACAGAGGACAGGCATGGCTTCCCCTTGGGCCTCCTCAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC228298 representing NM_001161442
Red=Cloning site Green=Tags(s)

MSPSTFQITDMTRRSCQNLGYTAASPQAPEAASNTGNAERAEEVPGEGSLFLQAETRAWFQKTQAHWLLQ
HGAAPAWFHGFITRREAERLLEPKPQGCYLVRFSESAVTFVLTYRSRTCCRHFLLAQLRDGRHVVLGEDS
AHARLQDLLLHYTAHPLSPYGETLTEPLARQTPEPAGLSLRTEESNFGSKSQDPNPQYSPIIKQGQAPVP
MQKEGAGEKEPSQLLRPKPPIPAKPQLPPEVYTIPVPRHRPAPRPKPSNPIYNEPDEPIAFYAMGRGSPG
EAPSNIYVEVEDEGLPATLGHPVLRKSWSRPVPGGQNTGGSQLHSENSVIGQGPPLPHQPPPAWRHTLPH
NLSRQVLQDRGQAWLPLGPPQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001161442
ORF Size 1113 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001161442.2
RefSeq ORF 1116 bp
Locus ID 9047
UniProt ID Q9NP31
Protein Pathways VEGF signaling pathway
MW 40.8 kDa
Gene Summary This gene encodes an adaptor protein thought to function in T-cell signal transduction. A related protein in mouse is responsible for the activation of lymphocyte-specific protein-tyrosine kinase and functions in downstream signaling. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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