KDM3A / JHDM2A (KDM3A) (NM_001146688) Human Tagged ORF Clone
CAT#: RC229072
KDM3A (Myc-DDK-tagged)-Human lysine (K)-specific demethylase 3A (KDM3A), transcript variant 2
ORF Plasmid: tGFP
"NM_001146688" in other vectors (6)
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CNY 9,200.00
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | JHDM2A; JHMD2A; JMJD1; JMJD1A; TSGA |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC229072 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCTCACGCTCGGAGAAAGTTGGCCGGTATTGGTGGGGAGGAGGTTTCTCAGTCTGTCCGCAGCCG ACGGCAGCGATGGCAGCCACGACAGCTGGGACGTGGAGCGCGTCGCCGAGTGGCCCTGGCTCTCCGGGAC CATTCGAGCTGTTTCCCACACCGACGTTACCAAGAAGGATCTGAAGGTGTGTGTGGAATTTGATGGGGAA TCTTGGAGGAAAAGAAGATGGATAGAAGTCTACAGCCTTCTAAGGAGAGCATTTTTAGTAGAACATAATT TGGTTTTAGCTGAACGAAAGTCACCTGAAATTTCTGAACGAATTGTACAGTGGCCTGCAATAACGTACAA ACCTCTGTTGGACAAAGCTGGTTTGGGATCCATAACTTCTGTTCGCTTTCTGGGAGATCAACAAAGAGTA TTTCTTTCTAAAGACCTTTTGAAGCCTATACAGGATGTAAACAGTCTTCGACTTTCTCTTACGGATAATC AGATTGTCAGTAAAGAATTTCAAGCTTTGATTGTGAAGCATTTAGATGAAAGCCATCTTTTAAAAGGTGA CAAAAACTTAGTTGGTTCAGAAGTAAAAATTTATAGCTTGGACCCATCTACTCAGTGGTTTTCAGCAACC GTTATAAATGGAAACCCAGCATCAAAAACTCTTCAAGTCAACTGTGAGGAGATTCCAGCACTGAAAATTG TTGATCCGTCACTGATTCATGTTGAAGTTGTACACGATAACCTTGTGACATGTGGTAATTCTGCAAGAAT TGGAGCTGTAAAACGCAAGTCTTCTGAGAATAATGGAACCCTGGTTTCCAAACAAGCAAAATCTTGCTCT GAGGCCTCTCCCAGTATGTGTCCTGTGCAGTCTGTACCTACAACAGTTTTTAAGGAGATACTGCTTGGCT GTACTGCGGCAACTCCACCTAGTAAGGACCCAAGACAGCAAAGTACTCCCCAGGCTGCCAACTCTCCACC TAACCTTGGAGCAAAAATTCCTCAAGGATGTCATAAACAAAGTTTACCAGAGGAAATTTCTTCCTGTCTA AATACAAAGTCTGAAGCTCTGAGAACAAAACCAGATGTCTGCAAAGCAGGGTTGCTCTCAAAGTCCTCTC AGATTGGAACTGGAGACTTGAAAATTCTGACTGAGCCAAAAGGCAGCTGTACTCAGCCTAAGACAAACAC TGATCAGGAAAACAGATTGGAGTCTGTTCCACAAGCATTGACTGGCCTTCCTAAGGAGTGCTTACCTACA AAGGCTTCTTCTAAGGCAGAATTGGAAATTGCCAATCCTCCTGAACTGCAGAAGCACCTAGAACATGCAC CTTCCCCATCGGATGTTTCAAATGCACCAGAAGTGAAAGCAGGTGTCAATAGTGATAGCCCTAATAACTG TTCAGGAAAAAAGGTAGAACCTTCAGCTTTAGCTTGCCGATCACAGAATTTAAAGGAATCTTCAGTAAAA GTAGATAATGAAAGCTGTTGTTCAAGAAGCAACAATAAAATCCAGAATGCCCCATCCAGGAAGTCGGTTT TGACAGACCCAGCTAAACTCAAAAAGCTGCAACAGAGTGGCGAGGCCTTCGTACAGGATGATTCTTGTGT GAACATCGTGGCACAGTTGCCTAAATGCCGAGAGTGTCGCTTGGACAGTCTCCGCAAGGATAAGGAGCAA CAGAAGGACTCACCTGTGTTTTGCCGCTTCTTTCACTTCAGGAGGTTACAATTCAACAAACATGGTGTGT TGCGGGTAGAAGGCTTCTTAACACCAAACAAGTATGACAATGAAGCAATTGGCTTGTGGTTACCTTTAAC CAAAAACGTTGTGGGGATTGATTTGGACACAGCAAAGTACATCTTGGCCAACATTGGAGACCACTTCTGT CAAATGGTGATTTCTGAAAAGGAAGCTATGTCAACTATTGAGCCACACAGACAGGTTGCTTGGAAGCGAG CTGTCAAAGGTGTTCGAGAAATGTGTGATGTGTGCGACACCACCATCTTCAACCTGCACTGGGTGTGTCC TCGGTGTGGGTTTGGAGTATGTGTGGACTGCTACCGGATGAAGAGAAAGAATTGCCAACAGGGTGCTGCT TACAAGACTTTCTCTTGGCTAAAATGTGTGAAGAGTCAGATACATGAACCAGAGAACTTAATGCCCACAC AGATCATTCCTGGAAAAGCACTCTATGATGTTGGAGACATTGTTCATTCTGTAAGAGCGAAATGGGGAAT AAAGGCAAACTGCCCTTGTTCAAACAGGCAATTCAAACTCTTTTCAAAGCCAGCCTCAAAGGAAGACCTA AAACAGACTTCTTTAGCTGGAGAAAAACCGACTCTTGGTGCAGTGCTCCAGCAGAATCCCTCAGTGTTGG AGCCAGCAGCTGTGGGTGGGGAAGCAGCCTCCAAGCCAGCCGGCAGCATGAAGCCTGCCTGTCCAGCCAG CACATCTCCTCTAAACTGGCTGGCCGACCTAACCAGCGGGAATGTCAACAAGGAAAACAAGGAAAAACAA CCAACAATGCCAATTTTAAAGAATGAAATCAAATGCCTTCCACCCCTCCCACCTTTAAGCAAATCCAGCA CAGTCCTCCATACGTTTAACAGCACAATTTTGACACCCGTAAGCAACAACAATTCTGGTTTCCTCCGGAA TCTCTTGAATTCTTCTACAGGAAAGACAGAAAATGGACTCAAGAATACACCAAAAATCCTTGATGACATC TTTGCCTCTTTGGTGCAAAATAAGACGACTTCTGATTTATCTAAGAGGCCTCAAGGACTAACCATCAAGC CCAGCATTCTGGGCTTTGACACTCCTCACTATTGGCTTTGTGATAATCGCTTGCTGTGCTTGCAAGACCC CAACAATAAGAGCAACTGGAATGTGTTTAGGGAGTGCTGGAAACAAGGGCAGCCAGTGATGGTGTCTGGA GTGCATCATAAATTGAACTCTGAACTTTGGAAACCTGAATCCTTCAGGAAAGAGTTTGGTGAGCAGGAAG TAGACCTAGTTAATTGTAGGACCAATGAAATCATCACAGGAGCCACAGTAGGAGACTTCTGGGATGGATT TGAAGATGTTCCAAATCGTTTGAAAAATGAAAAAGAACCAATGGTGTTGAAACTTAAGGACTGGCCACCA GGAGAAGATTTTAGAGATATGATGCCTTCCAGGTTTGATGATCTGATGGCCAACATTCCACTGCCCGAGT ACACAAGGCGAGATGGCAAACTGAATTTGGCCTCTAGGCTGCCAAACTACTTTGTTCGGCCAGATCTGGG CCCCAAGATGTATAATGCTTATGGATTAATCACTCCTGAAGATCGGAAATATGGAACAACAAATCTTCAC TTAGATGTATCTGATGCAGCTAATGTCATGGTCTATGTGGGAATTCCCAAAGGACAGTGTGAGCAAGAAG AAGAAGTCCTTAAGACCATCCAAGATGGAGATTCTGACGAACTCACAATAAAGCGATTTATTGAAGGAAA AGAGAAGCCAGGAGCACTGTGGCACATATATGCTGCAAAGGACACGGAGAAGATAAGGGAATTTCTTAAA AAGGTATCAGAAGAGCAAGGTCAAGAAAACCCAGCAGACCACGATCCTATTCATGATCAAAGCTGGTATT TAGACCGATCATTAAGAAAACGTCTTCATCAAGAGTATGGAGTTCAAGGCTGGGCTATTGTACAGTTTCT TGGGGATGTGGTGTTTATCCCGGCAGGAGCTCCACATCAGGTTCATAACTTATATAGCTGCATCAAAGTG GCTGAAGATTTTGTTTCTCCAGAGCATGTTAAACACTGCTTCTGGCTTACTCAGGAATTCCGATATCTGT CACAGACTCATACCAATCACGAAGATAAATTACAGGTGAAGAATGTTATCTACCATGCAGTGAAAGATGC AGTTGCTATGCTGAAAGCCAGTGAATCCAGTTTTGGCAAACCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC229072 protein sequence
Red=Cloning site Green=Tags(s) MVLTLGESWPVLVGRRFLSLSAADGSDGSHDSWDVERVAEWPWLSGTIRAVSHTDVTKKDLKVCVEFDGE SWRKRRWIEVYSLLRRAFLVEHNLVLAERKSPEISERIVQWPAITYKPLLDKAGLGSITSVRFLGDQQRV FLSKDLLKPIQDVNSLRLSLTDNQIVSKEFQALIVKHLDESHLLKGDKNLVGSEVKIYSLDPSTQWFSAT VINGNPASKTLQVNCEEIPALKIVDPSLIHVEVVHDNLVTCGNSARIGAVKRKSSENNGTLVSKQAKSCS EASPSMCPVQSVPTTVFKEILLGCTAATPPSKDPRQQSTPQAANSPPNLGAKIPQGCHKQSLPEEISSCL NTKSEALRTKPDVCKAGLLSKSSQIGTGDLKILTEPKGSCTQPKTNTDQENRLESVPQALTGLPKECLPT KASSKAELEIANPPELQKHLEHAPSPSDVSNAPEVKAGVNSDSPNNCSGKKVEPSALACRSQNLKESSVK VDNESCCSRSNNKIQNAPSRKSVLTDPAKLKKLQQSGEAFVQDDSCVNIVAQLPKCRECRLDSLRKDKEQ QKDSPVFCRFFHFRRLQFNKHGVLRVEGFLTPNKYDNEAIGLWLPLTKNVVGIDLDTAKYILANIGDHFC QMVISEKEAMSTIEPHRQVAWKRAVKGVREMCDVCDTTIFNLHWVCPRCGFGVCVDCYRMKRKNCQQGAA YKTFSWLKCVKSQIHEPENLMPTQIIPGKALYDVGDIVHSVRAKWGIKANCPCSNRQFKLFSKPASKEDL KQTSLAGEKPTLGAVLQQNPSVLEPAAVGGEAASKPAGSMKPACPASTSPLNWLADLTSGNVNKENKEKQ PTMPILKNEIKCLPPLPPLSKSSTVLHTFNSTILTPVSNNNSGFLRNLLNSSTGKTENGLKNTPKILDDI FASLVQNKTTSDLSKRPQGLTIKPSILGFDTPHYWLCDNRLLCLQDPNNKSNWNVFRECWKQGQPVMVSG VHHKLNSELWKPESFRKEFGEQEVDLVNCRTNEIITGATVGDFWDGFEDVPNRLKNEKEPMVLKLKDWPP GEDFRDMMPSRFDDLMANIPLPEYTRRDGKLNLASRLPNYFVRPDLGPKMYNAYGLITPEDRKYGTTNLH LDVSDAANVMVYVGIPKGQCEQEEEVLKTIQDGDSDELTIKRFIEGKEKPGALWHIYAAKDTEKIREFLK KVSEEQGQENPADHDPIHDQSWYLDRSLRKRLHQEYGVQGWAIVQFLGDVVFIPAGAPHQVHNLYSCIKV AEDFVSPEHVKHCFWLTQEFRYLSQTHTNHEDKLQVKNVIYHAVKDAVAMLKASESSFGKP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001146688 |
ORF Size | 3963 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001146688.1, NP_001140160.1 |
RefSeq Size | 4638 bp |
RefSeq ORF | 3966 bp |
Locus ID | 55818 |
UniProt ID | Q9Y4C1 |
MW | 147.3 kDa |
Gene Summary | This gene encodes a zinc finger protein that contains a jumonji domain and may play a role in hormone-dependent transcriptional activation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC229072L1 | Lenti ORF clone of Human lysine (K)-specific demethylase 3A (KDM3A), transcript variant 2, Myc-DDK-tagged |
CNY 15,110.00 |
|
RC229072L2 | Lenti ORF clone of Human lysine (K)-specific demethylase 3A (KDM3A), transcript variant 2, mGFP tagged |
CNY 15,110.00 |
|
RC229072L3 | Lenti ORF clone of Human lysine (K)-specific demethylase 3A (KDM3A), transcript variant 2, Myc-DDK-tagged |
CNY 15,110.00 |
|
RC229072L4 | Lenti ORF clone of Human lysine (K)-specific demethylase 3A (KDM3A), transcript variant 2, mGFP tagged |
CNY 15,110.00 |
|
RG229072 | KDM3A (tGFP-tagged) - Human lysine (K)-specific demethylase 3A (KDM3A), transcript variant 2 |
CNY 14,540.00 |
|
SC327707 | KDM3A (untagged)-Human lysine (K)-specific demethylase 3A (KDM3A) transcript variant 2 |
CNY 25,460.00 |
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