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JMY (NM_152405) Human Tagged ORF Clone
CAT＃: RC229401
JMY (Myc-DDK-tagged)-Human junction mediating and regulatory protein, p53 cofactor (JMY)
ORF Plasmid: tGFP
"NM_152405" in other vectors (5)
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CNY 7,240.00
CNY 8,740.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | WHAMM2; WHDC1L3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC229401 representing NM_152405
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGTTCGCGCTGGAGGAGACGCTCGAGTCGGACTGGGTGGCTGTGCGGCCCCATGTGTTCGACGAGC GCGAGAAACACAAATTCGTCTTTATTGTGGCCTGGAACGAGATTGAGGGCAAGTTTGCCATAACCTGCCA CAACCGGACGGCCCAGAGGCAGAGGAGCGGCTCCCGGGAGCAAGCGGGGGCGCGAGGGGGCGCCGAGGCC GGCGGAGCTGCGTCCGACGGGAGCCGCGGGCCCGGCAGCCCGGCGGGCAGGGGTCGGCCCGAGGCCACTG CCTCTGCAACTCTGGTTAGGAGCCCCGGGCCCCGGCGGAGCTCGGCCTGGGCGGAGGGCGGCTCTCCTCG GAGCACTCGCAGCCTTCTGGGGGACCCGCGGCTGCGGAGTCCTGGCAGCAAAGGGGCGGAGAGTCGTCTT AGGAGCCCAGTGCGGGCCAAACCCATCCCGGGTCAGAAAACATCTGAAGCCGACGATGCGGCGGGGGCAG CCGCTGCAGCAGCCCGGCCGGCGCCCAGAGAGGCCCAGGTGTCCTCTGTACGGATAGTGAGCGCCTCTGG GACGGTCTCCGAGGAGATAGAGGTGCTGGAAATGGTGAAGGAGGACGAGGCACCTCTGGCGCTCTCGGAC GCGGAGCAGCCGCCGCCCGCCACCGAGCTGGAGTCTCCGGCCGAAGAGTGCAGCTGGGCCGGACTGTTTT CTTTCCAGGACCTGCGCGCCGTGCACCAGCAGCTGTGCTCGGTGAACTCGCAGTTGGAGCCGTGCCTGCC GGTGTTCCCCGAGGAACCTTCGGGCATGTGGACTGTGCTGTTTGGGGGCGCCCCCGAGATGACCGAGCAG GAAATCGACACTCTGTGTTACCAGCTCCAGGTCTACCTGGGCCACGGCCTGGACACCTGCGGCTGGAAGA TCCTCTCCCAGGTGCTCTTCACCGAGACCGATGATCCCGAGGAGTATTACGAAAGCCTCAGCGAGCTGCG GCAGAAGGGCTACGAAGAAGTGCTTCAGCGGGCCAGGAAGCGCATCCAGGAGCTCTTGGATAAGCACAAG AATACAGAGAGCATGGTGGAGCTTCTGGACTTGTATCAGTTGGAGGATGAAGCCTACAGCAGCCTTGCAG AAGCTACAACCGAACTCTATCAGTATTTACTACAGCCATTCCGAGACATGAGAGAACTTGCCATGCTACG AAGACAGCAGATCAAGATTTCCATGGAGAATGATTATCTGGGACCTCGAAGAATTGAGAGTCTACAAAAA GAAGATGCTGATTGGCAGCGGAAAGCTCACATGGCTGTACTGTCTATTCAAGATCTTACTGTCAAGTACT TTGAAATAACAGCTAAAGCTCAAAAAGCTGTGTATGATCGAATGCGAGCTGATCAGAAGAAATTTGGTAA AGCATCATGGGCAGCGGCTGCTGAACGGATGGAAAAACTCCAGTATGCAGTTTCTAAGGAAACTTTGCAG ATGATGAGAGCTAAAGAGATATGCTTGGAACAGCGGAAACATGCACTAAAGGAAGAGATGCAGAGTTTGC GGGGTGGTACAGAAGCGATAGCACGATTGGATCAGTTAGAAGCTGATTATTATGACCTGCAACTTCAGTT GTATGAAGTACAGTTTGAAATCTTGAAGTGTGAAGAGTTACTATTGACAGCGCAACTAGAAAGCATCAAA AGACTTATATCAGAAAAAAGAGATGAAGTGGTATACTATGACACTTACGAAAGCATGGAGGCCATGCTGG AGAAGGAAGAGATGGCAGCATCTGCGTACTTACAGAGAGAAGAGCTGCAGAAACTTCAGCAGAAAGCACG CCAGCTGGAAGCAAGACGTGGACGGGTTTCTGCCAAGAAATCCTACCTCAGAAATAAAAAGGAAATATGT ATTGCAAAACACAATGAAAAAATCCAACAGCGCACTCGGATTGAAGATGAATATAGAACCCATCACACAG TACAACTAAAGAGAGAAAAATTACATGATGAAGAAGAAAGAAAAAGTGCCTGGGTTAGCCAAGAGAGACA GAGAACACTGGATAGACTTCGAACATTTAAACAGAGGTATCCTGGGCAAGTCATACTTAAATCAACCAGA TTACGACTAGCTCATGCAAGAAGAAAAGGTGCAGCAAGTCCTGTTCTCCAAGAGGATCATTGTGACTCTT TACCAAGTGTGTTACAGGTAGAAGAGAAAACTGAAGAGGTGGGAGAAGGAAGAGTCAAGCGTGGGCCATC ACAGACAACAGAACCCCAGAGCCTTGTGCAACTTGAAGATACTTCATTAACACAACTTGAAGCCACCTCA TTACCTCTCAGTGGTGTTACCTCTGAACTGCCTCCCACTATATCTCTTCCACTTTTGAATAACAACCTCG AACCATGTTCTGTTACCATAAATCCACTCCCATCCCCTCTTCCTCCAACACCACCACCTCCCCCACCTCC TCCCCCTCCCCCACCACCACCACCTCTGCCTGTTGCTAAGGACAGTGGCCCAGAGACACTGGAGAAAGAT CTGCCTAGAAAGGAGGGGAATGAGAAGAGGATCCCAAAGTCAGCCAGTGCCCCCTCAGCACACCTCTTTG ACAGCAGCCAGCTGGTCAGTGCACGGAAGAAGCTCAGAAAGACTGCTGAAGGTTTGCAGAGGAGGAGAGT GAGCTCACCCATGGATGAGGTGCTAGCCTCCTTGAAGCGTGGTAGTTTTCATCTGAAAAAGGTTGAACAG CGAACTCTGCCTCCTTTTCCTGATGAAGATGATAGTAATAATATCTTGGCACAAATAAGGAAAGGGGTAA AATTGAAGAAGGTACAGAAGGATGTTTTGAGAGAATCCTTCACACTTCTACCCGATACAGACCCTCTAAC ACGGAGCATCCATGAAGCTCTTAGAAGAATTAAAGAAGCATCCCCAGAGTCAGAGGACGAAGAGGAGGCT TTACCTTGCACAGACTGGGAGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC229401 representing NM_152405
Red=Cloning site Green=Tags(s) MSFALEETLESDWVAVRPHVFDEREKHKFVFIVAWNEIEGKFAITCHNRTAQRQRSGSREQAGARGGAEA GGAASDGSRGPGSPAGRGRPEATASATLVRSPGPRRSSAWAEGGSPRSTRSLLGDPRLRSPGSKGAESRL RSPVRAKPIPGQKTSEADDAAGAAAAAARPAPREAQVSSVRIVSASGTVSEEIEVLEMVKEDEAPLALSD AEQPPPATELESPAEECSWAGLFSFQDLRAVHQQLCSVNSQLEPCLPVFPEEPSGMWTVLFGGAPEMTEQ EIDTLCYQLQVYLGHGLDTCGWKILSQVLFTETDDPEEYYESLSELRQKGYEEVLQRARKRIQELLDKHK NTESMVELLDLYQLEDEAYSSLAEATTELYQYLLQPFRDMRELAMLRRQQIKISMENDYLGPRRIESLQK EDADWQRKAHMAVLSIQDLTVKYFEITAKAQKAVYDRMRADQKKFGKASWAAAAERMEKLQYAVSKETLQ MMRAKEICLEQRKHALKEEMQSLRGGTEAIARLDQLEADYYDLQLQLYEVQFEILKCEELLLTAQLESIK RLISEKRDEVVYYDTYESMEAMLEKEEMAASAYLQREELQKLQQKARQLEARRGRVSAKKSYLRNKKEIC IAKHNEKIQQRTRIEDEYRTHHTVQLKREKLHDEEERKSAWVSQERQRTLDRLRTFKQRYPGQVILKSTR LRLAHARRKGAASPVLQEDHCDSLPSVLQVEEKTEEVGEGRVKRGPSQTTEPQSLVQLEDTSLTQLEATS LPLSGVTSELPPTISLPLLNNNLEPCSVTINPLPSPLPPTPPPPPPPPPPPPPPPLPVAKDSGPETLEKD LPRKEGNEKRIPKSASAPSAHLFDSSQLVSARKKLRKTAEGLQRRRVSSPMDEVLASLKRGSFHLKKVEQ RTLPPFPDEDDSNNILAQIRKGVKLKKVQKDVLRESFTLLPDTDPLTRSIHEALRRIKEASPESEDEEEA LPCTDWEN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_152405 |
| ORF Size | 2964 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_152405.3, NP_689618.3 |
| RefSeq ORF | 2967 bp |
| Locus ID | 133746 |
| UniProt ID | Q8N9B5 |
| MW | 111.3 kDa |
| Gene Summary | Acts both as a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics depending on conditions. In nucleus, acts as a cofactor that increases p53/TP53 response via its interaction with p300/EP300. Increases p53/TP53-dependent transcription and apoptosis, suggesting an important role in p53/TP53 stress response such as DNA damage. In cytoplasm, acts as a nucleation-promoting factor for both branched and unbranched actin filaments. Activates the Arp2/3 complex to induce branched actin filament networks. Also catalyzes actin polymerization in the absence of Arp2/3, creating unbranched filaments. Contributes to cell motility by controlling actin dynamics. May promote the rapid formation of a branched actin network by first nucleating new mother filaments and then activating Arp2/3 to branch off these filaments. The p53/TP53-cofactor and actin activator activities are regulated via its subcellular location (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
p53-dependent DNA repair during the DNA damage response requires actin nucleation by JMY
,null,
Cell Death and Differentiation
,PubMed ID 37142657
[JMY]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC229401L3 | Lenti ORF clone of Human junction mediating and regulatory protein, p53 cofactor (JMY), Myc-DDK-tagged |
CNY 10,640.00 |
|
| RC229401L4 | Lenti ORF clone of Human junction mediating and regulatory protein, p53 cofactor (JMY), mGFP tagged |
CNY 10,640.00 |
|
| RG229401 | JMY (tGFP-tagged) - Human junction mediating and regulatory protein, p53 cofactor (JMY) |
CNY 9,600.00 |
|
| SC318196 | JMY (untagged)-Human junction mediating and regulatory protein, p53 cofactor (JMY) |
CNY 19,000.00 |
|
| SC328036 | JMY (untagged)-Human junction mediating and regulatory protein p53 cofactor (JMY) |
CNY 19,000.00 |
