FNDC5 (NM_001171940) Human Tagged ORF Clone
CAT#: RC229535
- TrueORF®
FNDC5 (Myc-DDK-tagged)-Human fibronectin type III domain containing 5 (FNDC5), transcript variant 3
ORF Plasmid: tGFP
"NM_001171940" in other vectors (4)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | FRCP2; irisin |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC229535 representing NM_001171940
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATACACCCCGGGTCGCCGAGCGCCTGGCCGCCCCGCGCCCGCGCCGCGCTCCGCCTGTGGCTGGGCTGCG TCTGCTTCGCGCTGGTGCAGGCGGACAGTCCCTCAGCCCCAGTGAACGTCACCGTCAGGCACCTCAAGGC CAACTCTGCAGTGGTGAGCTGGGATGTTCTGGAGGATGAGGTTGTCATCGGATTTGCCATCTCCCAGCAG AAGAAGGATGTGCGGATGCTGCGCTTCATCCAGGAGGTGAACACCACCACCCGCTCATGTGCCCTCTGGG ACCTGGAGGAGGATACGGAGTACATAGTCCACGTGCAGGCCATCTCCATTCAGGGCCAGAGCCCAGCCAG CGAGCCTGTGCTCTTCAAGACCCCGCGTGAGGCTGAGAAGATGGCCTCCAAGAACAAAGATGAGGTAACC ATGAAAGAGATGGGGAGGAACCAACAGCTGCGGACAGGCGAGGTGCTGATCATCGTCGTGGTCCTGTTCA TGTGGGCAGGTGTCATTGCCCTCTTCTGCCGCCAGTATGACATCATTGAAGCG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC229535 representing NM_001171940
Red=Cloning site Green=Tags(s) IHPGSPSAWPPRARAALRLWLGCVCFALVQADSPSAPVNVTVRHLKANSAVVSWDVLEDEVVIGFAISQQ KKDVRMLRFIQEVNTTTRSCALWDLEEDTEYIVHVQAISIQGQSPASEPVLFKTPREAEKMASKNKDEVT MKEMGRNQQLRTGEVLIIVVVLFMWAGVIALFCRQYDIIEA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001171940 |
ORF Size | 543 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001171940.2 |
RefSeq Size | 2099 bp |
RefSeq ORF | 546 bp |
Locus ID | 252995 |
UniProt ID | Q8NAU1 |
Protein Families | Transmembrane |
MW | 20.3 kDa |
Gene Summary | This gene encodes a secreted protein that is released from muscle cells during exercise. The encoded protein may participate in the development of brown fat. Translation of the precursor protein initiates at a non-AUG start codon at a position that is conserved as an AUG start codon in other organisms. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC229535L3 | Lenti-ORF clone of FNDC5 (Myc-DDK-tagged)-Human fibronectin type III domain containing 5 (FNDC5), transcript variant 3 |
CNY 5,890.00 |
|
RC229535L4 | Lenti-ORF clone of FNDC5 (mGFP-tagged)-Human fibronectin type III domain containing 5 (FNDC5), transcript variant 3 |
CNY 5,890.00 |
|
RG229535 | FNDC5 (tGFP-tagged) - Human fibronectin type III domain containing 5 (FNDC5), transcript variant 3 |
CNY 4,370.00 |
|
SC328173 | FNDC5 (untagged)-Human fibronectin type III domain containing 5 (FNDC5) transcript variant 3 |
CNY 3,990.00 |