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YAP1 (NM_001195044) Human Tagged ORF Clone
CAT＃: RC231269
YAP1 (Myc-DDK-tagged)-Human Yes-associated protein 1 (YAP1), transcript variant 3
ORF Plasmid: tGFP
"NM_001195044" in other vectors (5)
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CNY 5,488.00
| Cited in 9 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | COB1; YAP; YAP2; YAP65; YKI |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC231269 representing NM_001195044
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCCCGGGCAGCAGCCGCCGCCTCAACCGGCCCCCCAGGGCCAAGGGCAGCCGCCTTCGCAGCCCC CGCAGGGGCAGGGCCCGCCGTCCGGACCCGGGCAACCGGCACCCGCGGCGACCCAGGCGGCGCCGCAGGC ACCCCCCGCCGGGCATCAGATCGTGCACGTCCGCGGGGACTCGGAGACCGACCTGGAGGCGCTCTTCAAC GCCGTCATGAACCCCAAGACGGCCAACGTGCCCCAGACCGTGCCCATGAGGCTCCGGAAGCTGCCCGACT CCTTCTTCAAGCCGCCGGAGCCCAAATCCCACTCCCGACAGGCCAGTACTGATGCAGGCACTGCAGGAGC CCTGACTCCACAGCATGTTCGAGCTCATTCCTCTCCAGCTTCTCTGCAGTTGGGAGCTGTTTCTCCTGGG ACACTGACCCCCACTGGAGTAGTCTCTGGCCCAGCAGCTACACCCACAGCTCAGCATCTTCGACAGTCTT CTTTTGAGATACCTGATGATGTACCTCTGCCAGCAGGTTGGGAGATGGCAAAGACATCTTCTGGTCAGAG ATACTTCTTAAATCACATCGATCAGACAACAACATGGCAGGACCCCAGGAAGGCCATGCTGTCCCAGATG AACGTCACAGCCCCCACCAGTCCACCAGTGCAGCAGAATATGATGAACTCGGCTTCAGGTCCTCTTCCTG ATGGATGGGAACAAGCCATGACTCAGGATGGAGAAATTTACTATATAAACCATAAGAACAAGACCACCTC TTGGCTAGACCCAAGGCTTGACCCTCGTTTTGCCATGAACCAGAGAATCAGTCAGAGTGCTCCAGTGAAA CAGCCACCACCCCTGGCTCCCCAGAGCCCACAGGGAGGCGTCATGGGTGGCAGCAACTCCAACCAGCAGC AACAGATGCGACTGCAGCAACTGCAGATGGAGAAGGAGAGGCTGCGGCTGAAACAGCAAGAACTGCTTCG GCAGGAGTTAGCCCTGCGTAGCCAGTTACCAACACTGGAGCAGGATGGTGGGACTCAAAATCCAGTGTCT TCTCCCGGGATGTCTCAGGAATTGAGAACAATGACGACCAATAGCTCAGATCCTTTCCTTAACAGTGGCA CCTATCACTCTCGAGATGAGAGTACAGACAGTGGACTAAGCATGAGCAGCTACAGTGTCCCTCGAACCCC AGATGACTTCCTGAACAGTGTGGATGAGATGGATACAGGTGATACTATCAACCAAAGCACCCTGCCCTCA CAGCAGAACCGTTTCCCAGACTACCTTGAAGCCATTCCTGGGACAAATGTGGACCTTGGAACACTGGAAG GAGATGGAATGAACATAGAAGGAGAGGAGCTGATGCCAAGTCTGCAGGAAGCTTTGAGTTCTGACATCCT TAATGACATGGAGTCTGTTTTGGCTGCCACCAAGCTAGATAAAGAAAGCTTTCTTACATGGTTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC231269 representing NM_001195044
Red=Cloning site Green=Tags(s) MDPGQQPPPQPAPQGQGQPPSQPPQGQGPPSGPGQPAPAATQAAPQAPPAGHQIVHVRGDSETDLEALFN AVMNPKTANVPQTVPMRLRKLPDSFFKPPEPKSHSRQASTDAGTAGALTPQHVRAHSSPASLQLGAVSPG TLTPTGVVSGPAATPTAQHLRQSSFEIPDDVPLPAGWEMAKTSSGQRYFLNHIDQTTTWQDPRKAMLSQM NVTAPTSPPVQQNMMNSASGPLPDGWEQAMTQDGEIYYINHKNKTTSWLDPRLDPRFAMNQRISQSAPVK QPPPLAPQSPQGGVMGGSNSNQQQQMRLQQLQMEKERLRLKQQELLRQELALRSQLPTLEQDGGTQNPVS SPGMSQELRTMTTNSSDPFLNSGTYHSRDESTDSGLSMSSYSVPRTPDDFLNSVDEMDTGDTINQSTLPS QQNRFPDYLEAIPGTNVDLGTLEGDGMNIEGEELMPSLQEALSSDILNDMESVLAATKLDKESFLTWL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001195044 |
| ORF Size | 1464 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001195044.2 |
| RefSeq ORF | 1467 bp |
| Locus ID | 10413 |
| UniProt ID | P46937 |
| Protein Families | Druggable Genome |
| MW | 53.2 kDa |
| Gene Summary | This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2013] |
Citations (9)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
CARMA3 Promotes Colorectal Cancer Cell Motility and Cancer Stemness via YAP-Mediated NF-κB Activation
,null,
Cancers
,PubMed ID 34885061
[YAP1]
|
|
Remodeling cancer stemness by collagen/fibronectin via the AKT and CDC42 signaling pathway crosstalk in glioma
,Zhong, C;Tao, B;Tang, F;Yang, X;Peng, T;You, J;Xia, K;Xia, X;Chen, L;Peng, L;,
Theranostics
,PubMed ID 33408794
[YAP1]
|
|
RNF181 modulates Hippo signaling and triple negative breast cancer progression
,Zhou, R;Ding, Y;Xue, M;Xiong, B;Zhuang, T;,
Cancer Cell Int.
,PubMed ID 32655323
[YAP1]
|
|
Molecular Mechanisms of Tyrosine Kinase Inhibitor Resistance Induced by Membranous/Cytoplasmic/Nuclear Translocation of Epidermal Growth Factor Receptor
,Rong, X;Liang, Y;Han, Q;Zhao, Y;Jiang, G;Zhang, X;Lin, X;Liu, Y;Zhang, Y;Han, X;Zhang, M;Luo, Y;Li, P;Wei, L;Yan, T;Wang, E;,
J Thorac Oncol
,PubMed ID 31228625
[YAP1]
|
|
Post-translational inhibition of YAP oncogene expression by 4-hydroxynonenal in bladder cancer cells
,Cucci, MA;Compagnone, A;Daga, M;Grattarola, M;Ullio, C;Roetto, A;Palmieri, A;Rosa, AC;Argenziano, M;Cavalli, R;Simile, MM;Pascale, RM;Dianzani, C;Barrera, G;Pizzimenti, S;,
Free Radic. Biol. Med.
,PubMed ID 31207288
[YAP1]
|
|
HMGB1 contributes to adriamycin-induced cardiotoxicity via up-regulating autophagy
,Luo, P;Zhu, Y;Chen, M;Yan, H;Yang, B;Yang, X;He, Q;,
Toxicol. Lett.
,PubMed ID 29715512
[YAP1]
|
|
PD-L1 confers resistance to EGFR mutation-independent tyrosine kinase inhibitors in non-small cell lung cancer via upregulation of YAP1 expression
,Tung, JN;Lin, PL;Wang, YC;Wu, DW;Chen, CY;Lee, H;,
Oncotarget
,PubMed ID 29435131
[YAP1]
|
|
MiR-195 suppresses the metastasis and epithelial-mesenchymal transition of hepatocellular carcinoma by inhibiting YAP
,Yu, S;Jing, L;Yin, XR;Wang, MC;Chen, YM;Guo, Y;Nan, KJ;Han, LL;,
Oncotarget
,PubMed ID 29245938
[YAP1]
|
|
Post-translational deregulation of YAP1 is genetically controlled in rat liver cancer and determines the fate and stem-like behavior of the human disease
,Simile, MM;Latte, G;Demartis, MI;Brozzetti, S;Calvisi, DF;Porcu, A;Feo, CF;Seddaiu, MA;Daino, L;Berasain, C;Tomasi, ML;Avila, MA;Feo, F;Pascale, RM;,
Oncotarget
,PubMed ID 27359056
[YAP1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC231269L1 | Lenti-ORF clone of YAP1 (Myc-DDK-tagged)-Human Yes-associated protein 1 (YAP1), transcript variant 3 |
CNY 7,888.00 |
|
| RC231269L2 | Lenti-ORF clone of YAP1 (mGFP-tagged)-Human Yes-associated protein 1 (YAP1), transcript variant 3 |
CNY 7,888.00 |
|
| RC231269L3 | Lenti-ORF clone of YAP1 (Myc-DDK-tagged)-Human Yes-associated protein 1 (YAP1), transcript variant 3 |
CNY 6,180.00 |
|
| RC231269L4 | Lenti-ORF clone of YAP1 (mGFP-tagged)-Human Yes-associated protein 1 (YAP1), transcript variant 3 |
CNY 7,888.00 |
|
| RG231269 | YAP1 (tGFP-tagged) - Human Yes-associated protein 1 (YAP1), transcript variant 3 |
CNY 4,750.00 |
