Syntenin 2 (SDCBP2) (NM_001199784) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	SITAC; SITAC18; ST-2; ST2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC232388 representing NM_001199784 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCATCCCTGTACCCATCTCTAGAGGACCTAAAAGTGGACCAAGCCATTCAGGCCCAGGTCAGAGCCT CACCCAAGATGCCAGCCCTGCCAGTCCAGGCAACAGCCATTTCCCCACCACCAGTTTTGTACCCAAACTT GGCAGAACTGGAAAATTATATGGGTCTTTCCCTCTCCAGCCAAGAAGTCCAGGAGAGCCTGCTTCAGATT CCAGAGGGTGACAGTACAGCGGTCTCGGGCCCCGGGCCCGGCCAGATGGTGGCACCGGTAACCGGGTACA GCCTGGGCGTGCGGCGAGCTGAGATCAAGCCCGGGGTGCGCGAGATCCACCTGTGCAAGGACGAGCGCGG CAAGACCGGGCTGAGGCTGCGGAAGGTCGACCAGGGGCTCTTTGTGCAGTTGGTCCAGGCCAACACCCCT GCATCCCTTGTGGGGCTGCGCTTTGGGGACCAGCTCCTGCAGATTGACGGGCGTGACTGTGCTGGGTGGA GCTCGCACAAAGCCCATCAGGTGGTGAAGAAGGCATCAGGCGATAAGATTGTCGTGGTGGTTCGGGACAG GCCGTTCCAGCGGACTGTCACCATGCACAAGGACAGCATGGGCCACGTCGGCTTCGTGATCAAGAAGGGG AAGATTGTCTCTCTGGTCAAAGGGAGTTCTGCGGCCCGCAACGGGCTCCTCACCAACCACTACGTGTGTG AGGTGGACGGGCAGAATGTTATCGGGCTGAAGGACAAAAAGATCATGGAGATTCTGGCCACGGCTGGGAA CGTTGTCACCCTGACCATCATCCCCAGTGTGATCTACGAGCACATGGTCAAAAAGTTGCCTCCAGTCCTG CTCCACCACACCATGGACCACTCCATCCCAGATGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC232388 representing NM_001199784 Red=Cloning site Green=Tags(s) MSSLYPSLEDLKVDQAIQAQVRASPKMPALPVQATAISPPPVLYPNLAELENYMGLSLSSQEVQESLLQI PEGDSTAVSGPGPGQMVAPVTGYSLGVRRAEIKPGVREIHLCKDERGKTGLRLRKVDQGLFVQLVQANTP ASLVGLRFGDQLLQIDGRDCAGWSSHKAHQVVKKASGDKIVVVVRDRPFQRTVTMHKDSMGHVGFVIKKG KIVSLVKGSSAARNGLLTNHYVCEVDGQNVIGLKDKKIMEILATAGNVVTLTIIPSVIYEHMVKKLPPVL LHHTMDHSIPDA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001199784
ORF Size	876 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001199784.1, NP_001186713.1
RefSeq Size	1484 bp
RefSeq ORF	879 bp
Locus ID	27111
UniProt ID	Q9H190
MW	31.6 kDa
Gene Summary	The protein encoded by this gene contains two class II PDZ domains. PDZ domains facilitate protein-protein interactions by binding to the cytoplasmic C-terminus of transmembrane proteins, and PDZ-containing proteins mediate cell signaling and the organization of protein complexes. The encoded protein binds to phosphatidylinositol 4, 5-bisphosphate (PIP2) and plays a role in nuclear PIP2 organization and cell division. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Read-through transcription also exists between this gene and the upstream FKBP1A (FK506 binding protein 1A, 12kDa) gene, as represented in GeneID:100528031. [provided by RefSeq, Sep 2011]

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